Presence of the genus Cuon in upper Pleistocene and initial Holocene sites of the Iberian Peninsula: new remains identified in archaeological contexts of the Mediterranean region

The dhole (Cuon alpinus) has been documented on the Iberian Peninsula throughout the Middle and Upper Pleistocene in archaeological and palaeontological contexts. However, this is an uncommon species among the Iberian quaternary faunae as a whole. This paper focuses on the remains of the genus Cuon recovered in three different sites of the Iberian Mediterranean region and their morphological and osteometrical study. By comparing these with fourteen complete skeletons of Canis lupus, as well as with other remains of C. alpinus, we intend to highlight some anatomical and metrical differences between Cuon and Canis that, added to those already published, could be a functional base-line for the identification of this genus. The identification of this species is complex, especially in archaeological contexts, and in some cases the dhole has been identified as Canis or Canidae. Furthermore, this paper explores the chronology of the presence of the dhole in the Iberian Peninsula and the different contexts where these animals have been recovered from.     

Reappraisal of ‘chronospecies’ and the use of Arvicola (Rodentia,Mammalia) for biochronology

The water vole, genus Arvicola, is characterised by a broad geographic distribution throughout Europe and is widespread during the late Middle and Upper Pleistocene. This genus is used as a major biostratigraphic tool within the Quaternary. Specific determinations using the Schmelzband-Differenzierung-Quotient or SDQ have identified many chronospecies within the fossil species Arvicola cantiana (Hinton, 1910). As SDQ calculation remains limited, this study reappraises the Arvicola genus in terms of morphodiversity and morphospace using outline analysis which takes into account the tooth as a whole. Outline analysis suggests that one single species of Arvicola, A. cantiana, was present during the Pleistocene. This species shows great variability with no trends or patterns in morphospace. Thus, these results call into question the reliability of SDQ for specific determinations and throw doubt on the biochronological framework based on Arvicola.     

Independent confirmation of a diagnostic sheep/goat peptide sequence through DNA analysis and further exploration of its taxonomic utility within the Bovidae

Buckley et al. (2010) recently identified a Type I collagen (COL1A2) peptide sequence that discriminates between sheep and goat species. The exact location of this peptide sequence on the genome was not reported. We identified the sequence's location and developed a PCR based approach that amplifies the diagnostic sequence from exon 41 of the COL1A2 gene. Using DNA analysis, we confirmed that this COL1A2 peptide discriminates between goat and sheep species reliably, but is limited as a more general phylogenetic marker.     

First dendroarchaeological dates of prehistoric contexts in South America: chullpas in the Central Andes

Despite the existence of long-lived tree species with excellent tree-ring characteristics, the use of dendrochronological techniques for dating archaeological contexts has been very limited in South America. Recent research in the Bolivian Altiplano of the Central Andes has yielded a network of Polylepis tarapacana chronologies that goes back to the 13th century. This species was regularly used by local populations since pre-Columbian times as raw material for beams, lintels, hooks and other architectural features in houses, storage chambers, and sepulchers. The aim of this study is to argue in favor of the potential of P. tarapacana for providing high resolution dates of significant archaeological events during the pre-Columbian era. The chronology of chullpas (burial towers and storage chambers) in the southern Andean Altiplano is used as a case study. Growth rings from P. tarapacana have provided several centennial-length dendrochronological records including a reference regional chronology covering the last 786 years in the Central Andean highlands. Based on this long reference chronology calendar years were assigned to woody pieces from pre-Hispanic chullpas. Dendroarchaeological results are consistent with contextual evidence and most radiocarbon dates from woody samples and related archaeological items. Our results indicate that P. tarapacana has a high potential for providing annually-resolved tree-ring dates for archaeological contexts in the Altiplano since the early 13th century.     

Radiocarbon and U-series dating of the endemic deer Praemegaceros cazioti (Depéret) from “Grotta Juntu”, Sardinia

Radiocarbon and U-series methods (²³⁰Th/U and ²³¹Pa/²³⁵U) for absolute age determination have been applied to some fossil samples from Grotta Juntu, in North Eastern Sardinia (Italy). The remains belong to the endemic deer species Praemegaceros cazioti, which is here represented by an almost complete skeleton. The three dating methods lead to concordant ages of about 7500 years BP, indicating that the skeleton was maintained as closed system after burial. Taking into consideration these results, Praemegaceros cazioti from Grotta Juntu is now the youngest representative of this species in Sardinia.     

Tikal timbers and temples: ancient Maya agroforestry and the end of time

Tikal, a major lowland Maya civic-ceremonial center in the heart of the Petén region of Central America, relied heavily on the adjacent lowland rainforest as a resource base for fuel and construction materials. In this study, we analyzed 135 wood samples from timbers used in the construction of all six of the city's major temples as well as two major palaces to determine which tree species were being exploited and to better understand ancient Maya agroforestry practices during the Late Classic period. We found evidence for a change in preference from the large-growing, upland forest species, Manilkara zapota, to a seasonal wetland species Haematoxylon campechianum in A.D. 741 as well as a decrease in lintel beam widths over time. Though M. zapota later returned as the wood species of choice in A.D. 810, beam widths were found to be significantly smaller. These findings concur with models that hypothesize widespread deforestation during the Late Classic period and indicate a declining forest resource base by the 9th century A.D. Because of the many large timbers available for temple construction in the 8th century, some system of forest conservation is indicated for the ancient Maya prior to the Late Classic period.     

Morphological and genetic studies of waterlogged Prunus species from the Roman vicus Tasgetium (Eschenz,Switzerland)

Morphological and genetic studies were performed on waterlogged Prunus fruit stones from the Roman vicus Tasgetium (Eschenz, Switzerland). Some fruit stones could be identified to species level based on morphological and metric criteria. Other fruit stones found were not identifiable to species level. Of the latter, the morphological group Prunus insititia/spinosa represents either native sloe, cultivated primitive plum (damson) or a hybrid of both. In one out of 10 individual fruit stones of this group Prunus specific chloroplast trnL-trnF and nuclear ITS1 markers were verifiably amplified. Sequences of trnL-trnF led to the identification of Prunus spinosa. The presence of authentic DNA was confirmed by phylogenetically meaningful sequences from the archaeobotanical group Prunus avium/cerasus of the same sample using chloroplast rbcL and nuclear ITS1 DNA markers. The results demonstrate the utility of waterlogged plant remains for genetic analysis: as seeds of fruit trees are often preserved waterlogged in the archaeological record. Ancient DNA (aDNA) studies are a promising tool to answer archaeobotanical issues concerning early horticulture, which probably started in the Northern alpine region with the onset of Romanisation.     

Testing flora as bioindicator of buried structures in the archaeological area of Maxentius’s villa (Rome,Italy)

The flora of the archaeological area of Maxentius’s villa (Rome) was tested as bioindicator of buried remains of masonry and pavements. A total of 53 floristic surveys provided the occurrence of each species in sample units, as well as some their phenological features. The data were elaborated through multivariate statistical analysis in order to assess floristic affinities/differences among sample units, and define their ecological characteristics. The fuzzy set methodology was applied to evaluate relation between floristic richness, plant cover and soil depth. Results showed that the buried remains of stonework create a discontinuity in the ground, influencing the flora growing above it. This phenomenon may give rise to differential presence of some species (e.g., Cota tinctoria, Trifolium scabrum ssp. scabrum on thin soil; Ranunculus bulbosus, Trifolium pratense on deeper soil), but will not have such a great effect on floristic richness. The bioindication of this phenomenon also occurs through phenological irregularities in single specimens and changes in plant cover. Nevertheless other environmental factors (soil moisture/aridity, trampling, enrichment of nitrogen, cutting, ground depression) can interfere with the use of plants as bioindicators of buried structures. It is therefore necessary to consider other factors ecological influencing each site when you want to use plants as bioindicators in archaeological prospection.     

Oxygen isotopic composition of limpet shells from the Beagle Channel: implications for seasonal studies in shell middens of Tierra del Fuego

Shell middens distributed along the coastal areas of the Beagle Channel (Tierra del Fuego) testify the primary role of marine resources in the successful social dynamic of hunter–gatherer–fisher societies (HGF) in extreme high latitude environmental conditions. Intertidal and subtidal limpet species are frequently identified taxa of these archaeological deposits. We investigated whether shell δ¹⁸O of the limpet species Nacella deaurata is a valuable record of local sea surface temperature (SST) and ambient water δ¹⁸O (δ¹⁸Ow) fluctuations, and in turn can be used to estimate the season of its exploitation by Holocene HGF. Sequential shell δ¹⁸O of modern species track seasonal changes of SST and δ¹⁸Ow. The comparison of shell δ¹⁸O profiles of N. deaurata and Nacella magellanica (a coexisting species) suggests the occurrence of distinct growth rate and physiological adaptations between species. Shell δ¹⁸O of zooarchaeological specimens of N. deaurata from Lanashuaia II suggests that animals were exploited predominantly in winter and that experienced similar environmental conditions of present day at ∼1320 years BP. Results also indicate that N. deaurata is a valuable candidate for studying past seasonal changes in SST and δ¹⁸Ow in this southernmost part of South America.     

Starch grain characterization of Prosopis chilensis (Mol.) Stuntz and P. flexuosa DC,and the analysis of their archaeological remains in Andean South America

The fruit (pods) of Prosopis (Fabaceae) are frequently recovered from pre-Hispanic Argentinian archaeological sites, suggesting that this genus was of importance in ancient economies in this region. Yet it is only recently that archaeobotanists have begun to carry out systematic research into this genus. Therefore many questions remain to be addressed concerning the food value of Prosopis fruit, and its potential contribution. This paper examines starch from the pods of two species, Prosopis flexuosa and Prosopis chilensis, for the purposes of describing and classifying their morphological features and biometrical parameters. Pods of both species were gathered from two extremes (northern and southern) of the Hualfín Valley, Catamarca, Argentina. Starch abundance is estimated and compared with that of Zea mays, an economically important plant with high concentrations of starch. This paper reveals that Prosopis pods contain very low amounts of starch compared to starch-rich edible species. Despite this, the recovery of Prosopis starch grains can be successfully used to assess archaeological tool uses. Prosopis starch grains were found to be highly variable in shape. Grain size ranged between 10 and 20 μm. Granule irregularity and the high birefringence under polarized light are two of the most important diagnostic features. No significant statistical differences were found in the structure (morphology, size and hilum) of the starch of the same species from both localities. Finally, the implications for the role of this plant in past societies are discussed.     

DNA analysis of archaeological rabbit remains from the American Southwest

Ancient DNA analysis was carried out on 20 archaeological rabbit remains from an early Pueblo II period site in Colorado (circa 1000 A.D.) to explore the possibility of obtaining accurate rabbit genus and species identifications. The presence of abundant rabbit remains at archaeological sites in the American Southwest indicates the importance of rabbit species in the subsistence economy and ritual activities of early aboriginal populations. The study of these remains is hindered by the difficulty of accurate identification due to the fragmentary nature of the bones and the lack of genus- and species-specific morphological features.A short cytochrome b gene fragment was amplified and sequenced to produce a genetic profile for each bone sample. At the genus level, the DNA identifications were consistent with those based on the analysis of mandible morphology for the majority of specimens. When compared to species-specific reference DNA sequences, Lepus americanus and Lepus californicus samples were easily identified. Identification of an unexpected L. americanus (snowshoe hare) from the remains provided new information concerning hunting ranges or exchange between groups in the region. Sylvilagus nuttallii and Sylvilagus audubonii, however, could not be confidently differentiated at this point due to the difficulty in obtaining accurate species-specific reference sequences.The inability to obtain such reference sequences can be a serious problem for DNA species identification of non-domestic animals that lack population-level genetic data and have few sequences available in GenBank. The lack of the DNA data increases the possibility that inappropriate reference sequences could be applied, resulting in false species identification even when authentic DNA is retrieved and amplified from ancient remains.     

Some archaeoherpetological remains from the Netherlands

A reference collection of amphibian and reptile bones of the species currently found in the Netherlands is used for the identification of herpetological material from three excavations in the province of North Holland. The presence of the following species could be demonstrated: Rana temporaria L., R. arvalis Nilsson, Bufo bufo L., B. calamita Laur., Natrix natrix L. and Emys orbicularis (L.). These findings imply new records for R. arvalis and E. orbicularis, compared with their present distribution. Ecological implications of these results are discussed.     

Millet microremains—an alternative approach to understand cultivation and use of critical crops in Prehistory

The term “millets” is used to identify several genera of grasses (Poaceae), most of which belong to the subfamily Panicoideae. Millets are one of the major food sources in arid and semi-arid areas of the world and they have been important crops in the prehistory of Africa and Eurasia. In this paper, we discuss phytoliths and starch grains from two of the less studied major millets (Pennisetum glaucum and Sorghum bicolor) as well as from some small millet species that are not normally considered of much importance (so-called forgotten millets: Digitaria ciliaris, Echinochloa colona, Echinochloa frumentacea, Brachiaria ramosa, Setaria pumila and Setaria verticillata). The preliminary results of this study on phytolith morphology, both at single and joined (silica skeletons) morphotypes, and starch grains show great potentials for the identification of different genus or species on the basis of microremains.     

The impact of traditional land use on woodlands: a case study in the Spanish Central System

We analyse the consequences of traditional use on forest characteristics during the last millennium in a mountain area of Spanish Central System, using information contained in documents, as well as cartography and toponymy. In the Middle Ages the local forests were transformed into municipal dehesas (communal fenced woods), communal wood pastures and arable lands. Pinewoods located in areas above 1500 m decreased because of the high grazing pressure. In the fifteenth century two manors were created (Buitrago and Montesclaros). Livestock became an important factor during this period. As a consequence an open wood was formed and oak coppices were also abundant (the felling of this species was at that time permitted by the Ordinances). At the end of the period of traditional management, pinewoods were disappearing and the main forest areas were located only in municipal dehesas. The area of beech was reduced in favour of melojo oak and scrub, due to the lower sprouting capacity of the beeches and their lower drought tolerance. Open wood pastures remain only in the eastern part of the study area, where there has been less intense human intervention. After the abolition of manors in the nineteenth century, arable land increased in the western part of the area, which had a higher population density. In the twentieth century, both human and livestock pressure decreased substantially and pine species were reintroduced by reforestation. The primitive savannah was transformed into ‘infilled savannah’, while temperate species spread around their refuges in municipal woods.     

Use of vegetative remains in the identification of dyeplants from waterlogged 9th–10th century AD deposits at York

Vegetative remains of four dyeplants (Rubia tinctorum L., Genista tinctoria L., Diphasium complanatum (L.) Rothm. and Isatis tinctoria L.) found in 9th–10th century deposits at York, are described in detail. A fifth species (Reseda luteola L.), so far only found as seeds, is also described.     

Disentangling long bones of foxes (Vulpes vulpes and Alopex lagopus) from arctic archaeological sites

To discern the presence of two anatomically close species within an archaeological assemblage is always a problem for the analyst. A particularly interesting case is the distinction between the red fox (Vulpes vulpes) and the arctic fox (Alopex lagopus), which is often determined using non-rigorous criteria and producing results that must be regarded with caution. Classical methods for separating these two species and determining sexual dimorphism, and eventually the sex ratio, such as analyses using uni- and bivariate plots, are based on arbitrarily fixed limits between sexes. In this text, a more robust statistical method termed mixture analysis (MA) is used to determine the species of limb bones from foxes. First, the MA is applied to a sample of each species using metric data taken from a collection of present-day fox skeletons. Afterwards, the MA is applied to archaeological samples dated to the Dorset period and retrieved from the Tayara site (near Salluit, South Hudson Strait, Nunavik). The results clearly demonstrate that the greatest length (GL) of long bones, especially the humerus and tibia, is the best measurement for distinguishing the species, followed by the distal breadth and the proximal breadth; and that GL is better suited for sexing foxes. The results of MA applied to the Tayara site collection lead to the conclusion that the red fox is present along with the arctic fox among the assemblage, and that there is no fox body size change during the last two millennia in the Eastern Arctic.     

The extinct large cranes of the North-West palaearctic

Bones of a large crane, comparable in size with the Sarus Crane, Grus antigone, have been found in the Late Pleistocene of Britain and France, neolithic of Germany, and Bronze and Iron Age sites in Britain. They occur together with bones of the Common Crane, G. grus. On zoogeographical grounds these are unlikely to be referable to G. antigone, and the name G. primigenia Milne-Edwards 1896 is available for the species.G. melitensis, a crane of similar large size from the Eemian interglacial of Malta differs in having a narrower tendinal bridge on the distal tibiotarsus, and a small slender coracoid that suggests that the powers of flight may have been reduced. On present evidence it has no link with the previous species other than that of size.     

Evaluating prehistoric finds of Arrhenatherum elatius var. bulbosum in north-western and central Europe with an emphasis on the first Neolithic finds in Northern Germany

The swollen basal internodes of the grass species Arrhenatherum elatius var. bulbosum (tuber oat grass) are recorded here for the first time for Neolithic Germany. These charred bulbs occurred in the Late Neolithic soil mantle of the megalithic tomb of Albersdorf-Brutkamp LA 5. They are interpreted as most probably originating from the natural vegetation on and around the grave mound. The bulbs were possibly charred in the course of a ritual fire. However, their use as gathered plants and their intentional deposition in a secondary burial ritual during the Late Neolithic cannot be excluded with any certainty. Identification criteria for Arrhenatherum bulbs as well as the ecological requirements of the species are introduced here. Furthermore, prehistoric bulb finds from north-western and central Europe, and different interpretations concerning the occurrence of Arrhenatherum in different archaeological contexts, are discussed. The compilation of finds from literature and excavation reports shows that bulbs of Arrhenatherum were found rather infrequently in the Neolithic. Most commonly, charred bulbs of A. elatius var. bulbosum are detected in Bronze Age cremation graves. In the Iron Age, however, they mainly occur in domestic sites. This shows that the interpretation of the plant remains is dependent on their archaeological context. A ritual meaning of the bulbs has to be considered in the interpretation, but they may also have contributed to people’s daily diet. This evaluation of bulb finds in prehistoric and historic contexts contributes to the debate on the relevance of plant gathering in early economies and in ritual activities.     

Isotopic analysis of the ecology of herbivores and carnivores from the Middle Pleistocene deposits of the Sierra De Atapuerca,northern Spain

Carbon and oxygen isotope values reveal resource partitioning among the large mammal fauna from three contemporaneous Middle Pleistocene hominid-bearing localities within the Sierra de Atapuerca (northern Spain). Carbon isotope values sampled from the tooth enamel of fauna present during Atapuerca Faunal Unit 6 show that a C₃-dominated ecosystem surrounded the area where fossils were preserved during this time. For the herbivores, Fallow deer isotope values are significantly different from Red deer and horses and show that this species did not forage in open environments at this locality. Red deer and horses show similar feeding strategies with less negative carbon values implying use of more open environments for these taxa. For the carnivores, carbon isotope values for Ursus deningeri are significantly different from either lions (Panthera leo) or foxes (Vulpes vulpes) and support the contention that this species is herbivorous. Special metabolic mechanisms involved in hibernation in U. deningeri might also have influenced its isotope values. The carbon isotope values of remaining carnivores were similar and suggest that each was typically a generalist carnivore, eating a wide variety of prey items. While the isotopic results generally correspond to ecology indicated by previous techniques, this study shows that isotope analyses can provide further insights that alternate techniques do not provide. Isotope analyses can help elucidate the ancient ecology of taxa present in the Sierra de Atapuerca during the Middle Pleistocene allowing for an accurate portrayal of the setting in which humans lived.