Migration and Viking Dublin: paleomobility and paleodiet through isotopic analyses

During the early medieval period in Ireland, Dublin was established as the largest Viking settlement on the island in the ninth century AD. A previous biodistance study has suggested that the population of the town consisted of a polyethnic amalgam of immigrant and indigenous. In this study, we use biogeochemistry to investigate paleomobility and paleodiet in archeological human remains from the ninth to eleventh century levels at the sites at Fishamble Street II (National Museum of Ireland excavation number E172), Fishamble Street III (E190) and John’s Lane (E173), as well as twelfth-century remains from Wood Quay (E132). Through radiogenic strontium isotope, stable oxygen, carbon, and nitrogen isotope, and elemental concentration analyses, we investigate the origins of the individuals who lived and died in early and late Viking Dublin. Mean archaeological human enamel and bone isotope values from Dublin are ⁸⁷Sr/⁸⁶Sr = 0.70975 ± 0.00139 (2σ, n = 22), δ¹³C_{carbonate(V-PDB)} = −14.8‰ ± 0.8‰ (1σ, n = 12), and δ¹⁸O_{carbonate(V-PDB)} = −7.2‰ ± 1.0‰ (1σ, n = 12). Archaeological human bone samples exhibit mean δ¹³C_{collagen(V-PDB)} = −20.8‰ ± 0.5‰ (1σ, n = 12) and mean δ¹⁵N_{collagen(AIR)} = +10.0‰ ± 1.7‰ (1σ, n = 12). Comparing these data with archaeological faunal data from Dublin and published data from northern Europe, we argue that there are no clear immigrants from other parts of the North Atlantic, although there is one clear outlier in both origins and diet. Overall, the relative homogeneity in both paleomobility and paleodiet may support models of acculturation in Viking Dublin, rather than a high number of first-generation immigrants or continued migration from Scandinavia.     

Diet and death in times of war: isotopic and osteological analysis of mummified human remains from southern Mongolia

This study presents the results of an isotopic analysis of nine naturally mummified individuals—three adults, two adolescents, one juvenile, and three infants—recovered from the Hets Mountain Cave site in southern Mongolia, where they had been secondarily deposited. All of the individuals show evidence of violent perimortem trauma, but no skeletal indicators of nutritional or disease-related stress. Multi-isotopic data (δ¹³C, δ¹⁵N, δ¹⁸O, ⁸⁷Sr/⁸⁶Sr, and ²⁰ⁿPb/²⁰⁴Pb) were characterized in multiple tissues from each individual when possible, in order to reconstruct diet composition and residential origin at different points in life. Specifically, δ¹³C and δ¹⁵N in bone carbonate and collagen (N = 8) and hair keratin (N = 4) were coupled with enamel carbonate δ¹⁸O and δ¹³C (N = 3) and enamel ⁸⁷Sr/⁸⁶Sr, and ²⁰ⁿPb/²⁰⁴Pb (N = 3) to assess diet and residential mobility in relation to skeletal indicators of health and trauma. Results are consistent with a persistence of mixed C₃/C₄ pastoral subsistence and general stability of diet composition over the life course, in contrast to contemporary accounts of widespread famine and a dependence on grains imported from China throughout the region. However, results also suggest that at least some individuals may have migrated to this region of southern Mongolia from elsewhere during life, meaning that their dietary isotopic profiles may not represent local subsistence patterns near the Hets Mountain Cave site. Overall, these results speak to the utility of life course oriented multi-isotopic analysis in complementing more top-down historical analyses in understanding variation in subsistence, nutrition, and migration in regions undergoing significant political and economic turmoil.     

Reconstructing northern Chinese Neolithic subsistence practices by isotopic analysis

Stable isotope signatures of both human and non-human animal bone samples indicate that Neolithic farmers of the Yellow and Wei River basins in China potentially cultivated millet for two reasons: as a staple for human consumption and as fodder for domesticated animals, specifically pigs, dogs, and perhaps chicken. Bone samples were analyzed from four Neolithic sites: Jiangzhai, Shijia, Xipo, and Kangjia, spanning the time period from 7000 to 4000 years ago. A combination of very high carbon isotope ratios (δ¹³C = −7.7 ± 0.4‰) and low nitrogen isotope ratios (δ¹⁵N = 7.5 ± 0.5‰) in samples of Xipo pig and dog bone suggests that these monogastric animals consumed substantial quantities of C₄ plants, almost certainly millets. In fact, the proportion of C₄ plants in animal diets appears to have been even greater than that in human diet. Stable isotope values (δ¹³C = −10.0 ± 0.8‰; δ¹⁵N = 8.3 ± 0.5‰) of human bone collagen recovered at Jiangzhai and Shijia indicate a staple role for millets, as well as the consumption of both wild and other non-C₄ domesticated plant foods. As millet agriculture and animal husbandry apparently depended on one another, a strong mutualism between them was likely established in northern China during the Neolithic. We propose that variable redistribution of agricultural products between humans and animals, depending on the availability of wild resources and annual fluctuations in agricultural output, helped ensure the stability of Neolithic human subsistence in the Yellow and Wei River basins.     

Applying new approaches to modeling diet and status: isotopic evidence for commoner resiliency and elite variability in the Classic Maya lowlands

Classic Maya states were characterized by a high degree of socioeconomic stratification. This paper investigates the degree to which status, as defined by grave goods and tomb construction, influenced dietary patterns of elites and commoners throughout the Classic Period (200–900/1000 AD) of the southern lowlands. We compile a database (N = 102) of previously-published stable isotope ratios (δ¹³C collagen, δ¹³C apatite, and δ¹⁵N collagen) from Maya bone mineral and collagen, and interrogate these data through two new isotopic modeling techniques: a simple carbon isotope model ( Kellner and Schoeninger, 2007; Froehle et al., 2010) and a multivariate isotope model ( Froehle et al., 2012). We find that Maya elite diet varied significantly through time in terms of maize consumption and trophic level, while commoner diet remained remarkably stable. These findings provide new information relevant to studies of ancient Maya class structure and to studies of subsistence strategies of the pre-Columbian Americas.     

Exploring subsistence and cultural complexes on the south coast of Papua New Guinea using palaeodietary analyses

We present the results of a palaeodietary study of a skeletal sample (～800–300 BP) from the south coast of Papua New Guinea (Nebira, site ACJ) using multiple stable isotope analysis of bone collagen. The carbon, nitrogen and sulphur stable isotope ratios of 28 individuals (n = 12 males, n = 13 females and n = 3 subadults) suggested the diet at Nebira was based on C₃ plants (likely starchy vegetable staples) and included protein resources from the surrounding forested areas and C₃/C₄ savannah grasslands such as wallaby and other wild animals. Domestic species (e.g. Sus scrofa, Canis familiaris and Gallus gallus) may also have been consumed but could not be differentiated from wild species by stable isotope analysis. There were no significant differences in stable isotope values between males and females, but the δ³⁴S values of the juveniles suggest they may have consumed varied protein resources. The sulphur stable isotope ratios indicate there was no discernable marine component in the diet of any of the individuals from Nebira. The stable isotope results are interpreted within a wider context of Papuan south coast trade and exchange systems in an attempt to understand local interaction in the region.     

Isotopic anthropology of rural German medieval diet: intra- and inter-population variability

This study investigates the diet of an eleventh century CE parish community located in northwestern Germany. We assessed the isotopic compositions of human (n = 24) and faunal (n = 17) bone collagen (δ ¹³C_col, δ ¹⁵N_col) and human structural carbonate (δ ¹³C_sc) using skeletal material recovered from the Dalheim cemetery. Traditional interpretation of the isotopic data indicates that Dalheim residents likely relied on a C₃ plant-based diet and consumed some terrestrial animal products without evidence of marine resource input in the diet. Bivariate and multivariate models used as an additional means to assess diet indicate minor consumption of C₄ plant foods in this community. The multivariate-isotope model identified regional similarities and differences in C₄ plant/marine food consumption and in dietary protein sources by comparing data from Dalheim with those of other medieval sites from the published literature. We did not observe sex differences in this population but differences in δ ¹⁵N_col suggest that juveniles consumed the lowest trophic level protein.     

Historical ecology of late Holocene sea otters (Enhydra lutris) from northern British Columbia: isotopic and zooarchaeological perspectives

We examined the isotopic composition (δ¹³C and δ¹⁵N) of sea otter (Enhydra lutris) bone collagen from ten late Holocene (ca. 5200 years BP–AD 1900) archaeological sites in northern British Columbia (BC), Canada. Because sea otters are now extinct from much of this region and have not fully recolonized their former range (e.g. Haida Gwaii and most of northern BC) these data represent an important first step towards better understanding sea otter foraging ecology in BC. The isotopic data suggest a diet composed primarily of benthic invertebrates, with a very low reliance on epibenthic fish. There is very low isotopic and thus inter-individual dietary variability in Holocene BC sea otters during the late Holocene. Furthermore, zooarchaeological abundance data suggest that otters represented a widespread and significant focus of aboriginal hunting practices on the northern BC coast during the mid- to late-Holocene. The consistent reliance on a small number of low-trophic level prey and limited dietary variability in Holocene BC sea otters may reflect top-down impacts on otter populations by aboriginal peoples. As part of our assessment of sea otter diet, we review trophic discrimination factors (Δ¹³C and Δ¹⁵N) for bone collagen from published literature and find marked variability, with mean values of +3.7 ± 1.6 for Δ¹³C (n = 21) and +3.6 ± 1.3 for Δ¹⁵N (n = 15).     

Paleodietary analysis of the prehistoric population of the Canary Islands inferred from stable isotopes (carbon,nitrogen and hydrogen) in bone collagen

Nitrogen and carbon isotope compositions were measured in the bone collagen from a total of 86 prehispanic samples of the Canary Islands, and hydrogen in 70, all of them with enough amount of bone collagen, and adequate N and C content. These samples belong to prehistoric population of El Hierro (n = 27), Tenerife (n = 18), and Gran Canaria (n = 41). Isotope compositions were also obtained for prehistoric and modern food resources that were likely consumed by these people. Marked differences were observed among the three islands regarding the three isotopes analyzed: the δ¹⁵N values were highest among the population of Gran Canaria (10.8‰ ± 0.9‰), who also showed the highest δD values (7 ± 8‰). The population of El Hierro showed the highest δ¹³C values (−18.6‰ ± 0.7‰). These data suggest a high consumption of marine products by the population from El Hierro, and also an important consumption of terrestrial meat or marine, piscivore fish, by the population from Gran Canaria, together with domesticated C₃ plants (barley and/or wheat), fruits of Ficus carica and other wild species, and goat products. Additionally, marked differences were observed between men and women, which suggest that women consumed a more vegetal-based diet, a finding which is in agreement with the higher proportion of teeth with carious lesions among women.     

Modeling skeletal traits and functions of the upper body: Comparing archaeological and anthropological material

The aim of this study is to look at upper body functional modifications caused by mechanical loading. We look at 4th lumbar vertebra as well as fibrous humeral musculoskeletal stress markers (MSMs). This study uses information provided by magnetic resonance images of living individuals from the University of Oulu Hospital data banks (N = 91), archaeological skeletons from Sweden (N = 54) and England (N = 61), and autopsied skeletal collection of early 20th century Finns in Natural History Museum, University of Helsinki (N = 48). The lumbar vertebrae and MSM are subjected to mechanical loading caused by the upper body weight and loads lifted and/or carried. We hypothesized that the vertebral size reflect body size, habitual mechanical loading and the overall skeletal robusticity as mechanical competence to withstand mechanical loading standardized to body size, which has decreased over millennia. For Helsinki material occupation, age and sex is known and the material was used in Niinimäki (2011). In the study by Niinimäki (2011) MSM were found to be affected by the intensity of muscular action as well as body size and age. This study is reviewed here in light of re-analysis of the data to follow the current anatomical understanding of the entheses as well as viewing MSMs as a part of upper body functional complex. Only fibrous entheses were included in the re-analysis. Furthermore, due to small number of females where activity intensity could be assessed, females were dropped from the re-analysis.     

Combined archaeomagnetic and thermoluminescence study of a brick kiln excavated at Fontanetto Po (Vercelli,Northern Italy)

A combined archaeomagnetic and thermoluminescence study was carried out as part of a rescue archaeological excavation on a kiln discovered during the installation of methane gas pipelines beneath a rice field, along the southern border of Fontanetto Po village (Vercelli province, Italy). A total of 23 independent brick samples have been collected, oriented in situ with an inclinometer; the use of magnetic and sun compass was not possible due to the existence of metallic tubes beneath the kiln and a plastic cover above it. Standard archaeomagnetic procedures have been used for the determination of the archaeomagnetic inclination and absolute geomagnetic intensity. Stepwise thermal demagnetization shows a very stable characteristic remanent magnetization and the calculated mean inclination of the 23 samples is I = 65.3° with α₉₅ = 2.4° and k = 156. Archaeointensity experiments have been performed using the classical Thellier method as modified by Coe, with regular partial thermoremanent magnetization (pTRM) checks. The cooling rate and remanence anisotropy effects upon thermoremanent magnetization (TRM) have been investigated in all the specimens. A total of 19 archaeointensity determinations (at specimen level) that correspond to linear NRM–TRM plots were used for the calculation of the site mean archaeointensity that is 46.4 ± 2.9 μT. Archaeomagnetic dating results show two possible dating intervals for the last 1000 years, calculated at 95% confidence interval: a first one from 1511 to 1614 AD, and a second one from 1768 to 1872 AD. Thermoluminescence (TL) study has been also performed on two brick samples from the kiln's internal wall, using conventional laboratory procedures. According to the thermoluminescence results the kiln's last usage lies between 1796 and 1914 AD. This age is in good agreement with the second dating interval obtained by the archaeomagnetic analysis. The combined archaeomagnetic and thermoluminescence results suggest that the last firing of the kiln could have occurred between the end of the 18th century and the beginning of 20th century.     

Carbon isotope fractionation between diet,breath CO2, and bioapatite in different mammals

The carbon isotope fractionation between tooth enamel bioapatite, breath CO₂, and diet was measured for voles, rabbits, pigs, and cattle on controlled diets. The measured fractionation (expressed as isotope enrichment ɛ*) between enamel and diet was 11.5 ± 0.3‰, 12.8 ± 0.7‰, 13.3 ± 0.3‰, and 14.6 ± 0.3‰ for these respective species. There is a 1:1 correlation between ɛ*_{breath–diet} and ɛ*_{enamel–diet} (r² = 0.94, p < 0.01), whereas our data do not resolve significant inter-species differences in ɛ*_{enamel–breath}. These findings are consistent with the hypothesis that inter-species differences in ɛ*_{bioapatite–diet} are primarily a result of differences in digestive physiology, rather than differences in the magnitude of fractionation between mineral and body fluid.     

Cereal grinding technology in ancient Mesopotamia: evidence from dental microwear

Dental microwear features in a sample of 10 human teeth from Tell Ashara and Tell Masaikh, two archaeological sites in the Middle Euphrates valley, Syria, were compared for possible evidence of a shift in grinding technology in Mesopotamia—parallel to the well-documented introduction of large rotary querns and watermills in the Graeco-Roman world. Two chronological subsets (Bronze Age, n = 4 and Late Roman/Islamic period, n = 6) differred substantially and features related to a more abrasive diet (broad lines, pits and punctures visible on SE micrographs) were significantly less frequent in the later subsample which may indicate that the shift in cereal grinding technology occured in Mesopotamia before the Late Roman period.     

The value of sieving of grave soil in the recovery of human remains: an experimental study of poorly preserved archaeological inhumations

The aim of this work is to assess the effects of sieving basal grave fills, following lifting of visible skeletal remains on the recovery of human bones and loose teeth in poorly preserved inhumation burials. The experiment was carried out during excavations at the 7th–9th century AD cemetery site at Whitby, England. Burials generally consisted of degraded bone fragments and/or soil stains. In each grave (N = 70 adult burials), all visible human remains were hand-collected on site; following this, the soil remaining in the grave was recovered and sieved successively through 8 mm, 4 mm and 2 mm meshes. No bone or dental fragments in the 2–4 mm fraction were identifiable to skeletal element. Approximately one third of the total weight of bone, and one third of the total numbers of teeth were recovered in the 4–8 mm and >8 mm sieved fractions. It is concluded that sieving down to 4 mm is adequate for recovery of adult skeletal remains at the study site. It would be useful to conduct similar sieving experiments at other archaeological cemetery sites to provide data on recovery when bone survival is good, and in subadult burials.     

Residential mobility and social identity in the periphery: strontium isotope analysis of archaeological tooth enamel from southeastern Arabia

Strontium isotope analysis represents an effective means of assessing mobility and reconstructing geographic residence patterns in archaeological populations. This biogeochemical technique was utilized to test the hypothesis that burgeoning interregional exchange networks and the occurrence of exotic grave goods in local tombs would correspond with a highly mobile population and a considerable immigrant presence during the Umm an-Nar (2700–2000 BC) period in the UAE. This region has been considered peripheral relative to larger civilizations in Mesopotamia and the Indus Valley, but played an important role as a major supplier of copper for the Persian Gulf. Individuals (n = 100) from six monumental Umm an-Nar tombs (Mowaihat; Tell Abraq; Umm an-Nar Island I, II, V; Unar 1) were selected to evaluate the geographic origins of tomb members. Mean ⁸⁷Sr/⁸⁶Sr ratios from local individuals interred at Mowaihat (0.708863 ± 0.000014; 1σ, n = 12), Tell Abraq (0.708873 ± 0.000020; 1σ, n = 27), Umm an-Nar Island (0.708902 ± 0.000079; 1σ, n = 33), and Unar 1 (0.708805 ± 0.000065; 1σ, n = 25) all display little isotopic variability, indicative of a population that was not highly mobile. However, coupled with archaeological evidence, three immigrants from Tell Abraq (n = 2) and Mowaihat (n = 1) identified by deviant strontium values suggest that this region was actively engaged in interregional interaction. Despite claims that these tombs acted as visible markers of territoriality legitimized by local ancestors buried within them, the presence of non-locals suggests that as commerce became increasingly important, definitions of kinship and social identity may have become more flexible to better meet the needs of the local community and those with whom they interacted.     

Provisioning Inka feasts at Tiwanaku, Bolivia: the geographic origins of camelids in the Pumapunku complex

While political integration can be achieved by many means, here we focus on the use of feasting and statecraft in the Inka Empire of the Andean Late Horizon (c. AD 1400-1532) in South America. In order to examine Inka political integration of the Lake Titicaca Basin of Bolivia, we examine paleomobility and paleodiet through radiogenic strontium and stable oxygen and carbon isotope data in archaeological camelid remains from the site of Tiwanaku. Mean radiogenic strontium isotope values from all archaeological camelid enamel and bone samples is ⁸⁷Sr/⁸⁶Sr = 0.70998 ± 0.00179 (1σ, n = 48), mean stable oxygen isotope values from a sub-set of archaeological camelid enamel and bone samples is δ¹⁸O_{carbonate (VPDB)} = −10.0‰ ± 2.6‰ (1σ, n = 18) and mean stable carbon isotope values from a sub-set of archaeological camelid enamel and bone samples is δ¹³C_{carbonate (VPDB)} = −9.0‰ ± 1.7‰ (1σ, n = 18). While many camelids consumed in these feasting events were likely local to the Lake Titicaca Basin, others came from a variety of different geologic zones, elucidating our understanding of Inka statecraft and the role of feasting in political integration in empires in the past.     

Evidence of early medieval trade and migration between Wales and the Mediterranean Sea region

Imported Mediterranean pottery recovered from 5th–7th century settlement sites along the south Wales coast indicates that trade and contact between Wales and Byzantium continued following the collapse of the Roman Empire in the early-5th century. It is hypothesised that people as well as pottery continued to travel to Wales from Byzantium, some of whom subsequently settled amongst the local communities. Strontium and oxygen isotope analysis was undertaken on human remains (n = 33 individuals) from four early medieval cemeteries from south Wales. The study identified individuals who may not have been local to the British Isles, thus demonstrating that the isotopic analysis of human remains from Wales can further our understanding of migration to Britain during the early medieval period.     

Spatial variation of biologically available strontium isotopes (Sr/Sr) in an archipelagic setting: a case study from the Caribbean

This paper presents the results of strontium (Sr) isotope analysis of modern and archaeological animal remains and modern plant samples from the Caribbean region. The goal is to assess if patterns of human mobility can be determined from the archaeological record in an archipelagic setting. The range of variability and spatial distribution of biologically available strontium isotope signatures is reported and data evaluated to determine if sufficient heterogeneity exists to permit the identification of mobility despite the presence of potentially large contributions of marine strontium in island and coastal ecosystems. The (is)landscape is divided into several sub-regions based on the age and lithology of underlying geology and the variability of ⁸⁷Sr/⁸⁶Sr ratios is reported as; mean ⁸⁷Sr/⁸⁶Sr ± 2 standard deviations, number of samples: 1) Volcanic and Intrusive rocks (0.7077 ± 0.0019; n = 162); 2) Cretaceous–Miocene Limestone (0.7085 ± 0.0009; n = 50); 3) Pliocene–Quaternary Limestone (0.7091 ± 0.0004; n = 54); 4) Sedimentary Deposits (0.7094 ± 0.0015; n = 16); 5) Metamorphic Deposits (0.7104 ± 0.0014; n = 6). There are substantial differences between expected ⁸⁷Sr/⁸⁶Sr values based on associated geology and measured ⁸⁷Sr/⁸⁶Sr in flora and fauna samples. These differences emphasize the importance of focusing on bioavailable strontium, as opposed to geological proxies, for estimations of Sr isotope ranges, especially in archipelagic environments. While some overlap exists between the ⁸⁷Sr/⁸⁶Sr ranges of certain sub-regions, other sub-regions possessed limited variation and could be distinguished based on strontium isotope data. This spatial patterning of biologically available strontium indicates that strontium isotope analyses can be successfully applied to the archaeological study of human mobility within the Caribbean region.     

Expanding ecomorphological methods: geometric morphometric analysis of Cervidae post-crania

This study presents ecomorphological methods for reconstructing paleohabitats using three-dimensional geometric morphometric analyses of Cervidae (deer and relatives) post-cranial elements. Cervids are often the most abundant taxon in Eurasian Plio-Pleistocene sites, yet their post-cranial remains are rarely utilized in paleoecological reconstructions. Cervids are found in a wide variety of habitats, and thus their ecomorphology spans an appropriate range to serve as a proxy for paleohabitat. Four morphological features are examined in this study; the calcaneus as a whole (n = 122), the medial margin of the patellar surface of the femur (n = 133), the lateral margin of the tibial plateau (n = 136), and the plantar margin of the third phalanx (n = 62). These features were chosen because they represent various aspects of cervid locomotion important for power generation, stability, and substrate interaction. For each feature, canonical variates analyses with cross-validations were used to assess how well landmark configurations distinguish among specimens from different habitat types. Cross-validations returned correct reclassifications rates ranging from 38.9% to 66.3% in a four-habitat system, with resubstitution rates of 55.4% to 79.1% correct. Most habitat groups were found to be significantly different at p < 0.0001 using permutations tests. Variation in these ecomorphological adaptations are explored via visualizations depicting “open” and “closed” habitat types, and hypotheses are presented for cervid functional morphology.     

The ‘Headless Romans’: multi-isotope investigations of an unusual burial ground from Roman Britain

Recent excavations at Driffield Terrace in York (Northern England) revealed an extremely unusual Romano-British cemetery of probably all-male composition, more than half of the individuals decapitated and with high incidence of other peri- and antemortem trauma. This paper presents the results of multi- (carbon, nitrogen, oxygen and strontium) isotopic analyses of bone and dentine collagen (n = 68) and tooth enamel (n = 18) which were conducted to obtain further information on the identity of these individuals and, more generally, in order to investigate the relationship between burial rite and geographical origin in a Roman provincial capital. The results show that the childhood origins of the “Headless Romans” were significantly more diverse than those of humans from other cemeteries in Roman York, but they demonstrate also that similar, unusual burial rites do not necessarily indicate a common geographical origin. Of particular interest were two individuals whose diet contained a significant proportion of C₄ plant (probably millet)-based protein. These are the first such isotope values observed in Britain from any archaeological time-period. Millet was not cultivated in the British Isles in antiquity and the results therefore demonstrate the value of palaeodietary data for assisting in isotopic mobility studies.     

Trace element concentrations in teeth – a modern Idaho baseline with implications for archeometry,forensics, and palaeontology

Concentrations of 69 minor and trace elements were measured in situ using laser ablation inductively-coupled plasma mass spectrometry in multiple teeth of modern herbivores (Cervus elaphus, Odocoileus hemionus, Odocoileus virginianus; n = 52), omnivores (Ursus americanus; n = 56), and carnivores (Felis concolor; n = 33; Canis lupus; n = 4) from Idaho, USA. On each tooth, two to five analyses were collected on enamel, primary dentine, and secondary dentine with spot diameters of 30–100 μm. Continuous 30-μm-wide line traverses were collected across deer, elk, and wolf teeth. Results confirm trace element concentrations below 1 ppm for many elements, including Li, Be, transition metals (excepting Mn, Fe and Zn), heavy metals (excepting Hg and Pb), the rare earths, and actinides. Strontium and Ba generally biopurify with increasing trophic level, but Ba shows decreasing discrimination with tissue type in the order secondary dentine – primary dentine – enamel. Most elements show strong zoning within tissues and variable trophic level behavior, so concentrations in omnivores rarely fall between herbivores and carnivores. Trace element mass balance suggests that soil and dust, not food, commonly deliver the largest trace element load in terrestrial carnivores and omnivores, even for elements that biopurify, like Ba. Use of trace elements for inferring past diets are likely compromised by unknown rates of dust and soil ingestion rather than indicating the degree of herbivory and carnivory. The low concentrations of many elements provide multiple sensitive markers of post-burial overprinting that can be used to discriminate altered vs. original biogenic compositions. REE and actinide trends do not directly follow bedrock compositions.