The periodontal disease status of the historical population of Assos

The science of anthropology obtains data on health, disease and death from ancient populations. Research on the skeletal remains of human teeth and surrounding tissues provides useful information on the evolutionary perspective of dental and periodontal diseases. The aim of this study was to determine the prevalence and severity of periodontal diseases in the skeletal remains of 33 Assos inhabitants of the 4th century BC. Three age groups were constituted for the studied population. Periodontal disease status was determined based on the textural and architectural variations of the interdental septum and the extent of bone loss. It was found that the prevalence of periodontal diseases increased with age. The severity of periodontal bone loss also increased with age and the mean alveolar bone loss between age groups was statistically significant (P = 0.004). This analysis of the dental health of ancient populations provides insights into the aetiology, patterns and distribution of periodontitis, which has a very complex disease pathogenesis. Copyright © 2005 John Wiley & Sons, Ltd.     

Reliability of the Assessment of Periodontal Disease in Historical Populations

Periodontal disease is the destruction of all portions of periodontium. However, only periodontitis, based on whether attachment loss has occurred, can be detected in archaeological material. Therefore, several methods have been used to assess the frequency of periodontitis in past populations. The main scope of this study is to determine whether diagnoses of periodontitis through various methods are consistent with diagnoses obtained using the radiographic method. The samples (35 individuals, 576 teeth) were selected from Radom Cemetery's collection (in use between 1790 and 1811). For each individual, a periodontal status assessment was made using the following parameters: (i) measuring the distance between the cement–enamel junction (CEJ) and the crest of the alveolar bone (AC); (ii) the degree of root exposure; (iii) presence of the inter‐dental septum; (iv) dental calculus; (v) scoring system on the texture of the alveolar bone (Kerr's method) and (vi) radiography. In the studies, a correlation was found between the radiographic images and Kerr's method (r = 0.92) and root furcation (r = 0.96). Increasing degree of change on the alveolar bone is connected with increases in the CEJ–AC distance. A relationship between the frequency of the prevalence of dental calculus and changes on the alveolar bone was found. It means that the radiographic method, which is not always possible to use in archaeological collections, can be replaced by the presented macroscopic methods. Copyright © 2016 John Wiley & Sons, Ltd.     

Dental calculus and other dental disease in a human skeleton of the Okhotsk Culture unearthed at Hamanaka-2 site,Rebun-Island,Hokkaido, Japan

A human skeleton of the Okhotsk Culture from the Hamanaka-2 site of Rebun Island, northern part of Hokkaido, was found with abnormally large deposits of dental calculus, especially on the right upper 2nd and 3rd molars. This may relate to the early loss of the corresponding right lower molars. Root apex cementum hyperplasia and resorption of alveolar bone due to periodontal disease were also observed; these may have been associated with the calculus. The severe dental calculus and other diseases observed appear to have nothing to do with the subsistence pattern and ethnological background of the Okhotsk Culture, but rather with the individual's poor oral hygiene and digestive dysfunction.     

Metastatic carcinoma: palaeopathology and differential diagnosis

A 62 year‐old contemporary white female diagnosed with metastatic carcinoma of the breast was examined after skeletonisation. She never received chemical, hormonal or radiation therapy. Because of the confirmed clinical diagnosis, lack of medicinal intervention, and quality of bone preservation, this specimen provides a comparative standard for metastatic carcinoma and differential diagnosis of idiopathic dry bone pathology. We detail gross and radiographic bone response to this disease and differentially diagnose the skeleton against Langerhans cell histiocytosis and multiple myeloma, conditions capable of modifying bone in characteristic patterns that may mimic metastatic carcinoma. Copyright © 2006 John Wiley & Sons, Ltd.     

Development and Seismic Behavior of Precast Concrete Beam-to-Column Connections

A new precast concrete beam-to-column connection for moment-resisting frames was developed in this study. Both longitudinal bar anchoring and lap splicing were used to achieve beam reinforcement continuity. Three full-scale beam-to-column connections, including a reference monolithic specimen, were investigated under reversal cyclic loading. The difference between the two precast specimens was the consideration of additional lap-splicing bars in the calculation of moment-resisting strength. Seismic performance was evaluated based on hysteretic behavior, strength, ductility, stiffness, and energy dissipation. The plastic hinge length of the specimens is also discussed. The results show that the proposed precast system performs satisfactorily under reversal cyclic loading compared with the monolithic specimen, and the additional lap-splicing bars can be included in the strength calculation using the plane cross-section assumption. Furthermore, the plastic hinge length of the proposed precast beam-to-column connection can be estimated using the models for monolithic specimens.     

Manual for the Preparation and Staining of Embedded Natural Dry Bone Tissue Sections for Microscopy

In the last decade, the use of light microscopy has been firmly established for the investigation of exhumated human bone tissue. As a rule, these remains cannot be decalcified; thus, they are most commonly prepared for microscopic analysis as ground thin sections. These ground sections are of great value in diagnosing disease, in estimating age or in assessing taphonomic alteration. As bone is sometimes fragile and can be damaged by the grinding process, the specimen is occasionally supported by an embedding medium. In contrast to the vast amount of research conducted on embedded and unembedded unstained bone material, the use of histological stains on undecalcified dry bone tissue has been long neglected. In this article, a new method for embedding, sawing and grinding dry bone tissue is presented. The produced sections are subsequently stained with haematoxylin. The results show that even ground sections of fragile bone can be made in a quick and easy manner. Staining these sections enhances the envisioning of micro‐architecture and taphonomical processes. In addition, the sections stay open for inspection under polarized light. The results were consistent throughout the used bone material. To keep the method as accessible and comprehensive as possible, a step‐wise manual is provided. An additional troubleshooting paragraph discusses the most often encountered problems and provides solutions. Copyright © 2011 John Wiley & Sons, Ltd.     

Diagnostic morphometric applicability of confocal laser scanning microscopy in Osteoarchaeology

The confocal laser scanning microscope (CLSM) has become an essential tool for a wide range of biological and clinical studies and imaging applications. The major imaging modes of the CLSM include single and serial optical sections from thick specimens (100 µm), multiple wavelength images and three‐dimensional reconstruction. Such images provide measurement of length, surface or volume of objects. Surprisingly, this advanced microscopic method has been very rarely used in anthropological research, although numerous clinical and basic science research projects showed its value to study, bone growth, bone micro‐architecture and 3D bone morphometry. We present the basic principles and advantages of CLSM and outline practical aspects of specimen preparation, image collection and digital image processing specifically appropriate for historical bone and hair material. Copyright © 2009 John Wiley & Sons, Ltd.     

Describing Archaeological Iguana Laurenti, 1768 (Squamata: Iguanidae) Populations: Size and Skeletal Maturity

The goal of this study was to provide tools to estimate size and maturity of the archaeological Iguana specimens on the basis of their skeletal remains. To achieve this, a reference of 40 Iguana osteological specimens from different collections was used to derive size prediction equations relying on bone measurements. The sequence of fusions of skeletal elements was also observed and found to be strictly identical in all observed specimens. These observations enable the size and skeletal maturity of an archaeological specimen to be determined on the basis of an isolated bone. Such data could be valuable in zooarchaeological studies, as is demonstrated using the case of a pre‐Columbian archaeological site in the Lesser Antilles. Copyright © 2015 John Wiley & Sons, Ltd.     

High performance liquid chromatography (HPLC) in the investigation of gout in palaeopathology

Gout is a disease caused by the abnormal accumulation of uric acid in the body, which can result in sodium urate crystals forming tophi at joints, with associated erosion of bone and cartilage. Only two examples of tophi have been reported from archaeological individuals, and the diagnosis of gout based on dry bone manifestations can be difficult. This paper presents preliminary results of a new technique to aid the diagnosis of gout in palaeopathology, namely high performance liquid chromatography (HPLC). Five archaeological skeletons with suspected gout (diagnosed using visual and radiological analysis) and three controls were analysed. Two of the gouty individuals had a white powder in their erosive lesions. HPLC showed the presence of uric acid in bone in four of the five individuals with evidence of gouty arthritis and was negative for uric acid in bone from the three controls. The white powder was also positive for uric acid. With reliance on the presence of articular erosions, cases of gout will be missed in archaeological human bone. HPLC measurement of uric acid could prove useful in the differential diagnosis of erosive arthropathy in archaeology. It may also be useful in identifying individuals with an increased body pool of uric acid, linked to conditions included in the term ‘metabolic syndrome’. As a result, HPLC uric acid measurement also has the potential to provide additional information on health and lifestyle in past communities. Copyright © 2008 John Wiley & Sons, Ltd.     

Experimental Investigation of Circular Reinforced Concrete Columns under Different Loading Histories

Three reinforced concrete (RC) circular column specimens without an effective concrete cover were tested under constant axial compressive as well as cyclic lateral loading. The seismic behavior of the specimens under different loading paths was examined with the objective of understanding the influence of displacement history sequence on the seismic behavior of the columns in near-fault earthquakes. The influence of displacement history sequence upon the hysteretic characteristics, stiffness degradation, lateral capacity, as well as energy dissipation analysis was conducted. The hoop strains of lateral reinforcement at varied column heights under cyclic loading were attained by means of 8–16 strain gauges attached along the hoops. Additionally, the characteristics of strain distribution were investigated in the transverse reinforcement. The results of strain distribution were evaluated with Mander’s confinement stress model and the distribution around the cross section. The length of the plastic hinge at the end of the specimen was evaluated by measurement as well as the inverse analysis. Finally, the deformation of the specimen, which includes the components of shear deformation, bending deformation and bonding-slip deformation, was evaluated and successfully separated.     

New methods for the identification of evidence for bitting on horse remains from archaeological sites

This article describes alterations to equid lower second premolars and diastemata from a series of known life history equids and a number of archaeological horse specimens from the British Iron Age. Two new methods for recording bit wear are proposed involving the analysis of the extent and morphology of enamel/dentine exposure on the anterior edge of LP2s and analysis of the extent of new bone formation and bone loss to the diastema of the mandible. It is suggested that when a bit is used on a horse it acts more frequently on the anterior margin of the LP2 than has previously been thought and that repeated contact between the bit and the LP2s and diastemata results in recognisable damage to these areas of the mouth.     

NISP, Bone Fragmentation, and the Measurement of Taxonomic Abundance

Zooarchaeologists have long recognized that the number of identified specimens (NISP) is dependent on the degree to which bones are fragmented, but attempts are rarely made to control for the effects of fragmentation on NISP. This paper provides insight into those effects by presenting both a formal model of the relationship between NISP and fragmentation and experimental data on that relationship. The experimental data have practical implications regarding the effectiveness of potential measures of bone fragmentation, suggesting that specimen size—which can be determined easily through digital image analysis—is more useful than other variables that have been or might be used as fragmentation measures.     

A juvenile human frontal bone from the French Upper Palaeolithic site of Lacave: significance and problems of interpretation

In 1902, a portion of a juvenile human frontal bone was excavated by Armand Viré from the cave of Lacave in the Dordogne valley of southwestern France. The specimen is clearly from an Upper Palaeolithic context, although problems in interpreting the stratigraphy and postdepositional history in the cave render it essentially impossible to determine whether it is from a Magdalenian or Solutrean context. The morphology of the specimen is modern, as would be expected considering its relatively late Upper Palaeolithic context. Examination of aspects of brow ridge and frontal sinus development, as well as metopic suture obliteration, allows estimation of a developmental age of approximately 7–8 years for the specimen. Sex is indeterminate, and there is no pathology present. Apart from serving as an example of the problems of interpreting fragmentary skeletal remains from early 20th century excavations, the Lacave frontal represents one of the relatively few subadult cranial specimens known from the European Upper Palaeolithic. As such it is a valuable addition to the fossil record of early modern humans from Europe. Copyright © 1999 John Wiley & Sons, Ltd.     

Different Preparation Techniques – Similar Results? On the Quality of Thin‐Ground Sections of Archaeological Bone

Palaeohistology as a valuable diagnostic instrument is dependent on the production of high‐quality thin‐ground sections from dry bone. The objective of this study was to consider technical differences and assess the qualitative outcomes of five techniques for preparing thin‐ground sections from dry archaeological bone. Established techniques with long follow‐up times and excellently documented results were compared with simpler and cheaper time‐saving techniques. Evaluations were made of the quality of thin sections obtained by one classical machine‐based embedding technique, two revised versions of the same technique, one manual moulding technique based on Frost's rapid technique and one manual hybrid technique. Five osteological specimens of differing quality were prepared following the manuals for these five techniques and examined microscopically with respect to a list of standardised histological and diagenetic parameters. Alterations in the specimens attributable to preparation effects were recorded, and observations were scored with reference to three criteria: section quality, technical quality and staining. The results show that embedding techniques are to prefer. Superglue should not be used as a mounting or embedding medium. Manual grinding comes with several limitations, and machine cutting and grinding are preferred. Haematoxylin staining can be successfully applied to embedded specimens, giving more information on microscopic diagenetic processes. A stepwise manual for a revision of the classical embedding technique is presented. The time required for producing sections using classical embedding techniques is shortened from 6 weeks to 3.7 days by refining the preparation/polymerization processes involved with no loss of osteological data. Copyright © 2014 John Wiley & Sons, Ltd.     

Adult scurvy in skeletal remains of late 19th century mineworkers in Kimberley,South Africa

Throughout history, scurvy has been a well‐known disease which develops due to restricted resources of fresh fruit and vegetables. The condition results from an extended limited intake of vitamin C. Although skeletal lesions associated with infantile scurvy have been well described by many authors, very little literature is available on adult scurvy and the resulting skeletal lesions. The purpose of this study was to investigate the skeletal remains of a 19th century mining population from Kimberley, South Africa, for any skeletal lesions that may be indicative of adult scurvy. Scurvy was well documented as being extremely prevalent in this population. The skeletal remains of 107 individuals, presumed to have died around 1898, were studied. The majority of these individuals were males between 19 and 45 years of age. It is likely that most individuals were migrant workers at the diamond mines. All bones were visually assessed for macroscopic indications of pathological bone alterations associated with healed scurvy. Bone samples were also taken from ambiguous lesions in order to perform histological investigations. Lesions indicative of possible healed adult scurvy were observed in 16 individuals. These lesions included bilateral ossified haematomas, osteoperiostitic bone changes and periodontal disease. Histological investigation confirmed the presence of ossified haematomas on the anterior tibiae of some individuals. Hospital records and historical documents describing the incidence of scurvy in the local hospitals and the daily diet of the black mine workers supported these findings. Copyright © 2009 John Wiley & Sons, Ltd.     

Schomburgk’s Chook: the entangled South Australian collections of a German naturalist

Focusing on the politics of museums, collections and the untold stories of the scientific ‘specimens’ that travelled between Germany and Australia, this article reconstructs the historical, interpersonal and geopolitical contexts that made it possible for the stuffed skin of an Australian malleefowl to become part of the collections of Berlin’s Museum für Naturkunde. The author enquires into the kinds of contexts that are habitually considered irrelevant when a specimen of natural history is treated as an object of taxonomic information only. In case of this particular specimen human and non-human history become entangled in ways that link the fate of this one small Australian bird to the German revolutionary generation of 1848, to Germany’s nineteenth-century colonial aspirations, to settler–Indigenous relations, to the cruel realities that underpinned the production of scientific knowledge in colonial Australia, and to a present-day interest in reconstructing Indigenous knowledges.     

The angle of femoral torsion: An impossible measurement?

Torsion of the femur (ante- or retroversion) is a normal feature of the bone. It is related to the angular difference in orientation between the proximal and distal articulations. Torsion has been studied, both in vivo and in dry bone, using a variety of techniques, from a traditional goniometer to radiographs and tomography. Problems with the accurate measurement of the angle of femoral torsion have been encountered by many workers, resulting in a wide range of reported values. A pilot study was undertaken to attempt to demonstrate the difficulties in measuring the angle in dry bone. It was demonstrated that, using standard equipment, measurement of this angle has very poor reproducibility. Is it possible, therefore, to measure the angle of femoral torsion accurately?     

A PRACTICAL MODEL FOR SEISMIC ANALYSIS OF REINFORCED CONCRETE SHEAR WALL BUILDINGS

A simple macro-model for reinforced concrete shear walls is proposed, which consists of spring elements representing flexure and shear behaviour. The model for flexural behaviour is based on section analysis, while the model for shear behaviour is based on key parameters of the flexural behaviour. Four wall test specimens are selected to evaluate the reliability of the model. Modelling parameters for the backbone curves and the hysteretic rules are examined by conducting static and time history analyses, with the hysteretic response of a test specimen compared to that calculated using the proposed model. Results show some differences between measured and calculated shear force versus shear distortion relationships, but the model is acceptable because the differences do not significantly affect calculated global response. Parametric studies are also conducted to examine the influence of modelling parameters on seismic demand and capacity, which are the major design parameters for structural performance evaluation. Differences due to variation in modelling parameters are not significant, further indicating that the proposed model is reasonable.     

Fluvial transport of bovid long bones fragmented by the feeding activities of hominins and carnivores

This study explores the hydraulic transportability of bovid long bone fragments created through hominin and carnivore carcass consumption in order to determine the effect of fluvial transport on the incidences of hominin- and carnivore-induced bone surface modifications. Transportability was determined using an oval race track flume and 311 long bone fragments from modern control collections of hominin- and carnivore-modified bone. Results show that the fluvial transport of long bone fragments is predicted by animal size class and bone specimen size, as measured by maximum cortical thickness, maximum length, and maximum width. All of these variables can be measured on fossil specimens. Long bone portion (midshaft, near-epiphysis, and epiphysis) does not affect transport and hydraulic transport does not substantially modify the incidences of tooth, percussion, and cut marking in transported or lag assemblages in low energy fluvial environments. Implications of this study are: 1) animal size classes, and cortical thickness, length, and width of long bone fragments can be used to identify fluvial winnowing in fossil assemblages; 2) analyses concerning the relative timing of hominin and carnivore carcass consumption based on the proportions of long bone fragments bearing tooth, percussion, or cut marks can be meaningfully applied to fossil assemblages deposited in low-energy fluvial environments.