Microscopy in Camillo Golgi's times

The research by Camillo Golgi in histology and pathology dates from 1865, the year in which he obtained his MD degree, to 1923, when his last scientific article was published. Beginning in the mid 1855s, microscope manufacturers in Europe started producing objectives based on the principle of immersion introduced in 1847 by Giovan Battista Amici. The immersion objectives greatly improved the resolution of microscopic observations at high magnifications. From 1860 to 1872, technological improvements in microscope optics and the practicality of their use provided a larger community of investigators effective tools needed to study the structure of the nervous system. This progress in microscopy was associated with the application of new histological techniques, mastered by the chromoargentic reaction introduced by Golgi in 1873. In 1872, further progress in microscopy stemmed from the application of notions of applied physics to the production of microscope optics. These developments in microscopy will be briefly reviewed here.     

Camillo Golgi's Scientific Biography

Born in Corteno, a tiny village in the province of Brescia, Camillo Golgi studied at the University of Pavia where he graduated in medicine in 1865 under the guidance of the psychiatrist Cesare Lombroso who sparked his vocation to study the brain. Golgi then began to learn histological techniques under the direction of the pathologist Giulio Bizzozero. In 1872 he moved to Abbiategrasso as chief of a hospital for chronic diseases. In a rudimentary laboratory he developed the silver-bichromate staining technique, the ‘black reaction’, which was a breakthrough for nervous tissue structure research. While in Abbiategrasso Golgi demonstrated the branching of the axons, and observed striatal and cortical lesions in a case of chorea. He returned to Pavia as Professor of Histology and General Pathology, and made a series of important discoveries that still bear his name: the Golgi tendon organ, the Golgi-Mazzoni corpuscles, another Golgi method to stain nerve cells based on the use of potassium dichromate and mercuric chloride, the canaliculi of the parietal cells of the gastric glands (Müller-Golgi tubules), the Golgi-Rezzonico myelin's annular apparatus (or Golgi-Rezzonico horny funnels), the cycle of malarian parasites (Golgi cycle), the relationship between recurrent malarian fever bouts and the multiplication of the Plasmodium in the blood (Golgi law), the relationship between the vascular pole of the Malpighian glomerulus and the distal tubule, the Golgi's pericellular nets and finally, and most importantly, the cytoplasmic ‘internal reticular apparatus’ (Golgi apparatus). In 1906 Golgi was awarded the Nobel prize for Medicine or Physiology. He died in Pavia on 21 January 1921.     

Culture and Truth: Nietzsche and Classical Philology

Several recent studies have returned to the famous controversy over the reception of Nietzsche’s Birth of Tragedy from the Spirit of Music (1872). By reinterpreting it within the immediate context of Germany in the early 1870s, James Whitman understands this controversy as a Methodenstreit within Classical Philology and James I. Porter claims that, through this controversy, Nietzsche developed an extensive critique of modern culture. I contend that Nietzsche’s reaction to the scholarly rejection of his first publication resulted in no immediate response on his behalf; rather, it led to three years of intense rethinking and strengthening of the position he took in The Birth of Tragedy. This is evidenced in his early published essays and notebooks of 1872–1875. From the first readers of these early notebooks, Karl Schlechta and Anni Anders, to its most recent interpreters, Richard T. Grey and Alexander Nehamas, these scholars are unanimous in understanding them as Nietzsche’s attempt to work through a number of conventional philosophical problems. I argue that Nietzsche developed in these essays and notebooks a type of criticism that broke away from all traditional philosophical problems and creatively introduced such notions as cultural horizon, background phenomena, and life as a philosophical measure — all of which would be further refined in his mature texts of the 1880s and underpin his innovative concepts of the will to power and eternal recurrence.     

Camillo Golgi's scientific biography

Born in Corteno, a tiny village in the province of Brescia, Camillo Golgi studied at the University of Pavia where he graduated in medicine in 1865 under the guidance of the psychiatrist Cesare Lombroso who sparked his vocation to study the brain. Golgi then began to learn histological techniques under the direction of the pathologist Giulio Bizzozero. In 1872 he moved to Abbiategrasso as chief of a hospital for chronic diseases. In a rudimentary laboratory he developed the silver-bichromate staining technique, the 'black reaction', which was a breakthrough for nervous tissue structure research. While in Abbiategrasso Golgi demonstrated the branching of the axons, and observed striatal and cortical lesions in a case of chorea. He returned to Pavia as Professor of Histology and General Pathology, and made a series of important discoveries that still bear his name: the Golgi tendon organ, the Golgi-Mazzoni corpuscles, another Golgi method to stain nerve cells based on the use of potassium dichromate and mercuric chloride, the canaliculi of the parietal cells of the gastric glands (Muller-Golgi tubules), the Golgi-Rezzonico myelin's annular apparatus (or Golgi-Rezzonico horny funnels), the cycle of malarian parasites (Golgi cycle), the relationship between recurrent malarian fever bouts and the multiplication of the Plasmodium in the blood (Golgi law), the relationship between the vascular pole of the Malpighian glomerulus and the distal tubule, the Golgi's pericellular nets and finally, and most importantly, the cytoplasmic 'internal reticular apparatus' (Golgi apparatus). In 1906 Golgi was awarded the Nobel prize for Medicine or Physiology. He died in Pavia on 21 January 1921.     

Kazimierz Kelles-Krauz (1872–1905): A Pioneering Scholar of Modern Nationalism

Abstract. This essay attempts to place the contributions of the sociologist Kazimierz Kelles-Krauz (1872–1905) within the history of the scholarship of nationalism. Though absent from present scholarly literature on the question, Kelles-Krauz's contribution to the study of nationalism was outstanding. The article advances three arguments: that Kelles-Krauz was among the first to treat modem nationalism as an object of serious study, that his approach was more typical of our day than his, and that his arguments anticipated many of the major positions of today's scholars. In order to defend the first claim, the article briefly considers the contributions of scholars who preceded Kelles-Krauz. In order to defend the second and third claims, the article briefly characterises Kelles-Krauz's sociological method and presents his answers to some of the central questions of today's scholarly literature on nationalism.     

Luis Simarro Lacabra [1851–1921]: From Golgi to Cajal Through Simarro,via Ranvier

Knowledge of cerebral structure and function in its modern form can be traced to the neurone doctrine based largely on the work of Santiago Ramón y Cajal [1852–1934] and his lifelong exploitation of the Golgi method. Cajal openly acknowledged his debt to the neuropsychiatrist Luis Simarro Lacabra [1851–1921] who introduced him to the method in 1887, and recalled that the sight of the silver-impregnated nerve cells was the turning point which led him to abandon general anatomy and concentrate on neurohistology. Simarro, who dissipated his free time in trying to improve not only the scientific but also the political world around him, was able to produce exciting Golgi preparations of the cerebral cortex after he returned from voluntary exile in Paris from 1880 to 1885. Certainly it was there that he learned the methods of experimental histology from Louis-Antoine Ranvier [1835–1922] whose laboratory exercises, in the guise of lectures, he attended assiduously.     

The Golgi Stain: Invention,Diffusion and Impact on Neurosciences

The black reaction, invented in 1873 by Camillo Golgi (1843-1926), was the first technique to reveal neurons in their entirety, i.e. with all their processes. This important development passed unnoticed at first and only received wide international attention after a long delay. The Golgi stain was widely employed for almost thirty years and was directly responsible for major advances in our knowledge of the microscopic anatomy of the nervous system, as well as in other fields of study. In the hands of other researchers, the black reaction provided vital evidence that helped to establish the neuron theory. The Golgi stain was almost forgotten in the period between the two World Wars, but the introduction of the electron microscope to neurocytological research revived its use around the middle of the twentieth century. Today, the black reaction is still used extensively not only in combination with electron microscopy, but also as an autonomous technique in studies on the evolution, ontogeny, and organization of the nervous system.     

The Golgi Stain: invention, diffusion and impact on neurosciences

The black reaction, invented in 1873 by Camillo Golgi (1843-1926, was the first technique to reveal neurons in their entirety, i.e. with all their processes. This important development passed unnoticed at first and only received wide international attention after a long delay. The Golgi stain was widely employed for almost thirty years and was directly responsible for major advances in our knowledge of the microscopic anatomy of the nervous system, as well as in other fields of study. In the hands of other researchers, the black reaction provided vital evidence that helped to establish the neuron theory. The Golgi stain was almost forgotten in the period between the two World Wars, but the introduction of the electron microscope to neurocytological resarch revived its use around the middle of the twentieth century. Today, the black reaction is still used extensively not only in combination with electron microscopy, but also as an autonomous technique in studies on the evolution, ontogeny, and organization of the nervous system.     

Machiavelli’s passions

In his years as Florentine Secretary, Machiavelli repeatedly faced the problem of interpreting the gestures and words of statesmen that were masters of deception. The strategy he developed to uncover these statesmen’s intentions was based on the analysis of human passions, on uncovering the fundamental trait that defined a man’s character. I apply Machiavelli’s strategy of interpretation to Machiavelli himself and uncover the irreconcilable disconnect which shaped his personal tragedy. On one hand, he related to his objects of desire by entirely abandoning himself to them, regardless of how unachievable they were. On the other, to obtain these objects of desire his analytical mind developed strategies which took pride in their adherence to what he called the “effectual truth of the matter.” His incapacity to reconsider his objectives in light of the means at his disposal and his tendency to transfer all of himself into his objectives determined Machiavelli’s successes and failures. He repeatedly failed when he had to set his own goals, or when he approached a problem with empathy. He succeeded when he was given precise and limited objectives and when he understood that he did not share the goals of those he was studying.     

Nineteenth-Century London in William Godwin's Diary

The Leverhulme-funded editing of William Godwin's diary aims to ‘to construct a picture of London's literary and extra-parliamentary political life’, following the diary's ‘remarkably detailed map of radical intellectual and political life in the turbulent period of the 1790s’ <http://www.politics.ox.ac.uk/research/projects/godwin_diary/>. However, this map also extends far beyond the 1790s, with the diary's total span reaching from 1788 until Godwin's death in 1836. Critics and biographers have long assumed that Godwin's radical phase was over by 1800, and London to him was only ever a meeting point for free-floating, alienated intellectuals. By contrast, this paper presents new evidence showing his immersion in the material conditions and popular politics of nineteenth-century London. Following Godwin's movements in 1810 from his home and shop in Skinner Street, his perambulations around the city, and visits to dine with fellow radicals and publishers the article examines his immersion in the material conditions and popular politics of nineteenth-century London. It sees him campaigning against the abominable conditions in nearby Smithfield market; joining street protests to demand the release of Burdett from the Tower; and meeting Cobbett in Newgate. Godwin's circulations, recorded in his diary, bring to our attention the cross-fertilization between philosophic and popular radicalism and compel us to re-think the relationship between the conversations at private dinners and the protests in the streets in order to locate and better understand the nineteenth-century metropolitan critical public sphere.     

The impossible interview with the man of the hidden biological structures. Interview by Paolo Mazzarello

This paper presents an "impossible interview" to Professor Camillo Golgi, placed in time in December 1906. The Italian Professor Golgi from Pavia has been awarded the Nobel Prize for Physiology or Medicine ex aequo with the Spanish anatomist Santiago Ramón y Cajal. Both scientists have obtained the award for their work on the anatomy of the nervous system. However, they have opposite views on the mechanisms underlying nervous functions. Golgi believes that the axons stained by his "black reaction" form a continuous anatomical or functional network along which nervous impulses propagate. Ramón y Cajal is the paladin of the neuron theory, a hypothesis questioned by Golgi in his Nobel lecture of Tuesday, December 11. After the ceremony, an independent journalist has interviewed Professor Golgi in the Grand Hotel in Stockholm. Excerpts about his education, his main scientific discoveries, and his personal life are here given (reconstructing the "impossible interview" on the basis of Golgi's original writings).     

Notes on contributors

Abstract

There are at least two options or approaches available to those who seek to evaluate Garibaldi's life in its entirety. The first option envisages Garibaldi as a revolutionary figure firmly devoted to the cause of the people and the advancement of human rights. The second sees him as putting his popularity in the service of a sovereign monarch, but managing nevertheless to salvage something of the ideals of his youth. There are indeed double aspects to Garibaldi, who was both republican and monarchist, simultaneously a rebel and a man of order. As a rebel he fought against kings, popes and emperors; as a man of order he relied on the effectiveness of temporary dictatorship (his own in Rome in 1849 and the king's dictatorship in 1860). He broke with Mazzini when he chose to pursue national unification in collaboration with the monarchy. That choice limited his freedom of action, and he felt betrayed when he became aware of the consequences in the last years of his life. Paradoxically, it is Mazzini's death in 1872 that released Garibaldi from his subjection to King Victor Emmanuel II, and allowed him to live out the last years of his life more or less at peace with himself as a socialist who put the well being of the people ahead of everything else.     

Book Review

Gustaf Retzius (1842–1919), the Swedish anatomist and anthropologist, and Camillo Golgi were contemporaries. They met on several occasions and came in closer contact when Golgi, together with Ramón y Cajal, was awarded the Nobel Prize in Physiology or Medicine in Stockholm in 1906. Retzius came from an illustrious family. His father was professor of anatomy at Karolinska Institutet and Gustaf himself made a fast career. At 35, he was appointed to a professorship in histology, especially created for him at Karolinska Institutet, and later he became professor of anatomy in the same institution. Retzius was exceedingly productive, and published more than 300 scientific papers, most of which dealt with the nervous system and sensory organs. The majority of these were included in his magnificent volumes Biologische Untersuchungen, Neue Folge (Biological Investigations, New Series), which appeared from 1890 to 1921, and in Das Gehörorgan der Wirbelthiere (“The Acoustic Organ of Vertebrates”, 1881 and 1884), which may be his internationally better known contribution. Much of his work, especially on invertebrates, was based on Ehrlich's methylene blue method, but he also used the Golgi method early on. Particularly his studies of the innervation of the sensory organs became of great importance for the support of the neuron doctrine. His standing internationally was reflected in his membership in many of the most prominent academies abroad, as well as in invitations to him to give a “Croonian Lecture” in 1908 and “The Huxley Lecture” in 1909.     

‘The Epitheatrical Cartoonist’: Matthew Somerville Morgan and the World of Theatre,Art and Journalism in Victorian London

This article examines the vibrant cultural milieu inhabited by one of Victorian Britain's most famous cartoonists, Matthew Somerville Morgan. Morgan is well-known as the cartoonist who attacked Queen Victoria's withdrawal from public life (and her associations with John Brown), and the lifestyle of Albert, Prince of Wales, in the short-lived rival to Punch: the Tomahawk. Likewise, his post-1870 career in New York as cartoonist of the ‘Caricature War’ over the 1872 Presidential elections, and involvement with ‘Buffalo’ Bill Cody have been well-studied. However, his involvement with the world of the 1860s Victorian stage – and the social circles in which he moved – have not been given close attention. This broader social, cultural, and economic context is essential to understanding Morgan's role as a cartoonist-critic of politics, class, gender and art in Victorian Britain. Special attention is given to the ways in which Morgan's work as a theatrical scene-painter informed his other pursuits, including his political cartoons for Fun, the Comic News and the Tomahawk. So central was the theatre to Morgan's life story that he may be appropriately described as an ‘epitheatrical’ figure. Indeed he is one of the most spectacular exemplars of the interconnected worlds of journalism, high art and theatre in Victorian London. The theatre provided him with the artistic and journalistic connections needed to raise himself above his lower-class origins; to move in ‘clubland’ and fashionable bohemian society; and to win an influential place in the key political and cultural debates of his age.     

The discovery of the Golgi apparatus

The existence of the cell organelle which is now known as Golgi apparatus or Golgi complex, or simply as 'the Golgi", was first reported by Camillo Golgi in 1898, when he described in nerve cells an 'internal reticular apparatus' impregnated by a variant of his chromoargentic staining. It soon became clear that the newly-identified cytoplasmic structure occurred in a variety of cell types. However, the reality of the organelle was questioned for decades, until it was finally ascertained with electron microscopy. The Golgi apparatus was destined to become a protagonist of the research in cytology and cell biology pursued in the second half of the twentieth century.     

From the Mind to the Brain: An Unusual Pathway

One of the most unrecognized aspects of Golgi's life was his deep interest in neuropsychiatry. From 1865 to 1868 he attended the Clinica per le Malattie Nervose e Mentali in Pavia directed by Cesare Lombroso, the founder of modern criminology. Golgi was involved in research on the etiology of psychiatric ailments. During this short period of time he produced significant theoretic advances in clinical psychiatry. However, very soon he started to criticize the conceptual approach as well as the nosological system proposed by his academic mentor. In July 1868 he left Lombroso's school in search for a more rational method of studying brain functions and diseases. In spite of his anatomical approach to the central nervous system, he always maintained curiosity in the phenomenology of functional and organic mental disorders. This predisposition is witnessed by his capability to relate clinical observations to neuropathological findings.     

Gustaf Retzius and Camillo Golgi

Gustaf Retzius (1842-1919), the Swedish anatomist and anthropologist, and Camillo Golgi were contemporaries. They met on several occasions and came in closer contact when Golgi, together with Ramon y Cajal, was awarded the Nobel Prize in Physiology or Medicine in Stockholm in 1906. Retzius came from an illustrious family. His father was professor of anatomy at Karolinska Institutet and Gustaf himself made a fast career. At 35, he was appointed to a professorship in histology, especially created for him at Karolinska Institutet, and later he became professor of anatomy in the same institution. Retzius was exceedingly productive, and published more than 300 scientific papers, most of which dealt with the nervous system and sensory organs. The majority of these were included in his magnificent volumes Biologische Untersuchungen, Neue Folge (Biological Investigation, New Series), which appeared from 1890 to 1921, and in Das Gehororgan der Wirbelthiere ("The Acoustic Organ of Vertebrates", 1881 and 1884), which may be his internationally better know contribution. Much of his work, especially on invertebrates, was based on Ehrlich's methylene blue method, but he also used the Golgi method early on. Particularly his studies of the innervation of the sensory organs became of great importance for the support of the neuron doctrine. His standing internationally was reflected in his membership in many of the most prominent academies abroad, as well as in invitations to him to give a "Croonian Lecture" in 1908 and "The Huxley Lecture" in 1909.