Wild or domesticated? An ancient DNA approach to canid species identification in South Africa's Western Cape Province

The fragmentary remains typical of archaeological fauna can prove impossible to identify when confronted with closely related sympatric species. In southern Africa domesticated dogs (Canis familiaris) and black-backed jackals (Canis mesomelas) pose just such a problem, rendering difficult an accurate assessment of the timing of arrival of domesticated dogs in the region. Contextual evidence has suggested that canid remains from four Later Stone Age archaeological sites were likely to be domesticated dogs rather than jackals, and were subjected to ancient DNA analyses to determine species. Every tested specimen proved to derive from black-backed jackals. These data provide not only an unexpected window on southern African prehistory, but also highlight the value in applying ancient DNA techniques to archaeological species identification.     

Evidence for variability among squash seeds from the Hoxie site (11CK4), Illinois

Uncarbonized Cucurbita pepo L. (squash) seed coats were recovered from the base of a fortification ditch in water-saturated contexts at the Hoxie Farm site (11CK4) in northeastern Illinois. A direct date on one specimen indicated association with the Huber phase occupation, or about 1600 A.D. Sizes were highly variable, suggesting the presence of two or more squash varieties. To explore that possibility, length and width measurements were obtained for 21 specimens. A simple hierarchical cluster analysis revealed the presence of three distinct seed size groups. The two smaller conform to sizes displayed by modern varieties of subspecies ovifera which is the type domesticated in North America. Sizes for the third group of large seeds fall outside the range of any modern subspecies ovifera and within the range for the Mexican domesticate subspecies pepo. Similar large sized seeds first appear in the archaeological record from the Midwest during the Late Mississippian times. The potential for long-term prehistoric cultivation of two distinct lineages in the Midwest, and problems inherent in the scenario are discussed.     

Estimating meagre (Argyrosomus regius) size from otoliths and vertebrae

This study presents a method for predicting meagre (Argyrosomus regius) body size (total length) from otoliths and vertebrae recovered from archaeological sites. The method involves regression equations calculated from a reference collection of 36 meagre skeletons and 113 meagre otoliths (sagitta) and allows the simultaneous estimation of original body size and minimum number of individuals (MNI) from archaeological bone structures. We selected the following measurements to predict meagre body size: greatest dorso-ventral height, greatest mediolateral breadth, and greatest anteroposterior length of the vertebrae centra; maximum anteroposterior length, medial anteroposterior length, and dorso-ventral height of the sagitta. Our results show that the original body size of meagre can be accurately predicted from many bone measurements (r² range: 0.921–0.992). We exemplify the use of the regressions in the assessment of size variation and MNI of meagre from four Portuguese Mesolithic sites. We show that regression results provide additional insight into the significant role that this fish played in the subsistence of coastal fisher-hunter-gatherers, who targeted medium-sized animals but were also capable of acquiring rather larger specimens.     

关于中国稻作起源证据的讨论与商榷

本文是对傅稻镰等人最近的文章批评中国栽培水稻起源研究缺乏证据、提出长江下游水稻栽培开始于公元前4000年这一观点一文的回应。我们首先考察中国从野生稻采集到水稻栽培过程研究的相关文献．进而从水稻形态学和考古学的角度对早期水稻遗存加以研究。讨论集中在以下三个方面：长江流域最早水稻栽培的时间：长江下游和淮河流域初期栽培水稻的遗存;考古遗存所见水稻粒形态大小变化的意义。我们也考察了傅文用来区分野生水稻和栽培水稻的几个标准：考古纪录中所见的未成熟稻遗存、水稻粒的增大和水稻粒的形态。根据已经发表的资料和我们对水稻的研究,我们认为到全新世早期（距今9000年前后）,南北中国的新石器时代居民就可能已经收获野生稻并开始水稻栽培了。     

Sources of variation in carnivore tooth-mark frequencies in a modern spotted hyena (Crocuta crocuta) den assemblage,Amboseli Park,Kenya

Distributions and frequencies of carnivore tooth-marks on large mammal long-bone fragments are commonly used to infer the timing of hominin and carnivore access to prey resources in archaeofaunal assemblages. The strength of these inferences, however, is limited by a broad and currently inexplicable range of tooth-mark frequencies across experimental and archaeological assemblages. Controlling for this variation first requires that the sources be identified. Several sources of variation are examined here in an analysis of tooth-marked bone recovered from a modern spotted hyena (Crocuta crocuta) den assemblage in Amboseli Park, Kenya. Results indicate that tooth-mark frequencies: (1) depend on fragment size, (2) vary across mammals of different size classes, (3) are highly variable across equivalent portions of different long-bone elements, and (4) on certain long-bone portions are correlated with bone density and can be depressed in archaeological assemblages subjected to density-mediated attrition. Stronger inferences based on tooth-mark frequencies will require that such variation be taken into consideration, and methods for doing so are suggested.     

Early North African Cattle Domestication and Its Ecological Setting: A Reassessment

Nearly four decades have passed since an independent North African centre for cattle domestication was first proposed in 1980, based on the Combined Prehistoric Expedition’s work in the Nabta Playa—Bir Kiseiba region of southern Egypt, and the initial rigorous debates between Andrew B. Smith and Fred Wendorf, Romuald Schild and Achilles Gautier. More recently, geneticists have entered the fray with determinations on the spread of haplotypes, and the timing thereof, that extend the scope and increase the complexity of the debate. Here, a new look at the botanical data and a re-analysis of the geology of Bir Kiseiba–Nabta Playa rejects the ecological foundations of the early African domestication model, while a detailed examination of the published osteological and radiometric data from the same area reveals a more nuanced picture than has been recognised to date. These results are placed into context by a wider review of the genetic and other archaeological evidence from the Western Desert of Northeast Africa, where no other cattle remains designated as domesticated have been found. It is concluded that (a) Bos remains from the early Holocene at Nabta Playa—Bir Kiseiba were those of hunted aurochs; (b) domesticated caprines were likely present in Northeast Africa before domesticated cattle; and (c) the domesticated cattle spreading across Northeast and northern Africa, including Nabta Playa—Bir Kiseiba, from the late seventh millennium BC or early sixth millennium BC onwards were descendants of Bos taurus domesticated in the Middle Euphrates area of the Middle East.     

Disentangling long bones of foxes (Vulpes vulpes and Alopex lagopus) from arctic archaeological sites

To discern the presence of two anatomically close species within an archaeological assemblage is always a problem for the analyst. A particularly interesting case is the distinction between the red fox (Vulpes vulpes) and the arctic fox (Alopex lagopus), which is often determined using non-rigorous criteria and producing results that must be regarded with caution. Classical methods for separating these two species and determining sexual dimorphism, and eventually the sex ratio, such as analyses using uni- and bivariate plots, are based on arbitrarily fixed limits between sexes. In this text, a more robust statistical method termed mixture analysis (MA) is used to determine the species of limb bones from foxes. First, the MA is applied to a sample of each species using metric data taken from a collection of present-day fox skeletons. Afterwards, the MA is applied to archaeological samples dated to the Dorset period and retrieved from the Tayara site (near Salluit, South Hudson Strait, Nunavik). The results clearly demonstrate that the greatest length (GL) of long bones, especially the humerus and tibia, is the best measurement for distinguishing the species, followed by the distal breadth and the proximal breadth; and that GL is better suited for sexing foxes. The results of MA applied to the Tayara site collection lead to the conclusion that the red fox is present along with the arctic fox among the assemblage, and that there is no fox body size change during the last two millennia in the Eastern Arctic.     

Burning damage and small-mammal human consumption in Quebrada del Real 1 (Cordoba, Argentina): an experimental approach

The zooarchaeological study of small-vertebrate consumption requires a taphonomical approach to differentiate animal bones that were incidentally incorporated from those that were intentionally exploited in the past human subsistence. In order to make this distinction, the relationship between archaeological small-rodent burned bones and prehistoric human behavior was explored using an experimental cooking study as a modern analogue. During the cooking experiment the entire carcasses of three guinea pigs (Cavia porcellus) and two yellow-toothed cavies (Galea leucoblephara) were placed in the coals of an open fire that simulate a real campfire, rotating their positions until the meat was completely cooked. Subsequently, the intensity of burning damage and the loss of skeletal elements were analyzed at macroscopical levels. The data was used to identify cooking evidence in the Ctenomyidae and Caviidae rodent bones recovered from Quebrada del Real 1 (ca. 6000-300 BP, Córdoba, Argentina). Remarkable similarities between the archaeological and analogical records were found, including the distinctive burning pattern on the distal extremities of the unmeaty long bones (e.g, radii and tibiae), the high frequency of broken incisor teeth and the loss of autopodium elements. Based on these comparative results, it is suggested that the small-rodent assemblages of QR1 were primary accumulated by humans though butchery, cooking and consumption related activities. Extending this study to other archaeological sites in South America may help to identify the prehistoric bone collectors of these small-animals.     

On some remains of dog (Canis familiaris) from the Mesolithic shell-middens of Muge,Portugal

The dog has a unique relationship with humans. This is demonstrated by the number of breeds that exist today and the important role that dogs play in human society. The archaeological record also shows that this relationship began long ago when groups of hunter–gatherers domesticated the wolf probably in several parts of the globe.     

Back from burn out: are experimentally charred grapevine pips too distorted to be characterized using morphometrics?

This article examines the impact of charring on the possibility to characterize grape pips, at compartment (wild versus domesticated) and cultivar level, using morphometrics. Two morphometric methods have been used, one based on linear measurements (traditional morphometrics) and one on elliptic Fourier transforms (EFT; morphogeometrics). Charring experiments were performed using a laboratory muffle furnace and various charring conditions. Despite the strong impact of heating, results showed that wild and domesticated Vitis seeds can be reliably discriminated using both traditional morphometrics and morphogeometrics, even when charring has been done at high temperature (450 °C). The characterization of charred pips at cultivar level using EFT is very powerful when the seeds are charred at 250 °C, but the risk of misclassification is, as expected, higher at 450 °C. Results suggest that the characterization at cultivar level should only be attempted with large assemblages of well preserved archaeological pips, and only after a first classification at compartment level. Our approach was applied on a case study of two assemblages of waterlogged and well preserved charred pips from the archaeological site of Lattara. The results are consistent both between the two morphometric methods and between waterlogged and charred remains.     

Transition From Wild to Domesticated Pearl Millet (Pennisetum glaucum) Revealed in Ceramic Temper at Three Middle Holocene Sites in Northern Mali

Imprints of domesticated pearl millet (Pennisetum glaucum (L.) R. Br.) spikelets, observed as temper in ceramics dating to the third millennium BC, provide the earliest evidence for the cultivation and domestication process of this crop in northern Mali. Additional sherds from the same region dating to the fifth and fourth millennium BC were examined and found to have pearl millet chaff with wild morphologies. In addition to studying sherds by stereomicroscopy and subjecting surface casts to scanning electron microscopy (SEM), we also deployed X-ray microcomputed tomography (microCT) on eleven sherds. This significantly augmented the total dataset of archaeological pearl millet chaff remains from which to document the use of the wild pearl millet as ceramic temper and the evolution of its morphology over time. Grain sizes were also estimated from spikelets preserved in the ceramics. Altogether, we are now able to chart the evolution of domesticated pearl millet in western Africa using three characteristics: the evolution of nonshattering stalked involucres; the appearance of multiple spikelet involucres, usually paired spikelets; and the increase in grain size. By the fourth millennium BC, average grain breadth had increased by 28%, although spikelet features otherwise resemble the wild type. In the third millennium BC, the average width of seeds is 38% greater than that of wild seeds, while other qualitative features of domestication are indicated by the presence of paired spikelets and the appearance of nondehiscent, stalked involucres. Nonshattering spikelets had probably become fixed by around 2000 BC, while increases in average grain size continued into the second millennium BC. These data now provide a robust sequence for the morphological evolution of domesticated pearl millet, the first indigenous crop domesticated in western Africa.

     

Shape Change and Variation in the Cranial Morphology of Wild Canids (Canis lupus,Canis latrans,Canis rufus) Compared to Domestic Dogs (Canis familiaris) Using Geometric Morphometrics

Wild canid populations exhibit different anatomical morphologies compared to domesticated dogs in North America. This is particularly important concerning archaeological sites, which may contain early domesticated species, for the proper identification of osteological remains. Previous studies have indicated domestic dogs exhibit a shorter rostrum accompanied by a crowded tooth row; however, none describe the overall complexity of these changes. Consequently, using a landmark‐based geometric morphometric analysis, cranial morphological characteristics were examined in North American wild canids: the gray wolf (Canis lupus), coyote (Canis latrans), red wolf (Canis rufus), and the domestic dog (Canis familiaris). The shape and size of the cranium in lateral and ventral views were compared between the three wild species to the group of domesticated dogs. Wild canids clustered separately from the domestic group in all statistical analyses. Results indicate an expansion of the orbital region, a compression of the rostrum, and an overall warping in the shape and orientation of the skull. In domestic species, there is also a downward shift in the frontal portion of the skull accompanied by the braincase assuming a more upward position. This technique successfully depicted how slight changes in isolated areas of the cranium can have an impact on the overall shape and morphology of the skull. We presume these changes in cranial anatomy reflect the recent selective pressures domestic dogs have undergone since diverging from their wild ancestors. Copyright © 2012 John Wiley & Sons, Ltd.     

An Analysis of the Gut Contents of Five Well-Preserved Human Bodies from Tarapacá, Northern Chile

The detailed microscopic analysis of the gut contents from five well-preserved desiccated human bodies, dated between 540 BC and AD 600, from the Tarapacá quebrada, Northern Chile, has been undertaken. This analysis indicated that local wild resources such as the seeds of Chenopodium sp., pods of Prosopis sp. and the underground organs of Schoenoplectus/Scirpus sp. made up a substantial part of the diet even after the presence of domesticated species can be demonstrated for the area. Evidence for the consumption of fish was also recovered but the identification of larger mammals such as the Andean camelids in the diet is only tentative. This stands in contrast to the data recovered from other classes of archaeological remains and highlights the need to correlate as many different classes of complementary data as possible if a more complete picture of ancient diet is to be forthcoming.     

A systematic approach to the recovery and identification of starches from carbonised deposits on ceramic vessels

Starch granules are being successfully recovered from an increasing range of artefacts. Here we present the recovery of starches from carbonised ceramic ‘foodcrusts’ from late Mesolithic–early Neolithic residues at the site of Neustadt in northern Germany. A method for investigating background loading of residues with contaminant starches is proposed by comparing interior ‘foodcrusts’ versus exterior ‘sooting’, for the purposes of eliminating samples with insignificant quantities of grains from subsequent identification procedures. The classification of starches to plant taxon is traditionally achieved by manual observations and measurement of nominal and ratio morphological variables. Here, we present a method for the automated classification of granules, using software developed in-house. The results show that when multiple granules are considered, the species selected as modern reference examples can be classified to high levels of specificity. When applied to the archaeological samples we show that wild plant resources persist in importance across the transition to agriculture, with high proportions of granule forms consistent with acorn (Quercus sp.) occurring in all samples. Hazelnut (Corylus avellana) types are less well-represented suggesting it was not an important food in the context of pottery, and may have been over-represented in the repertoire of hunter–gatherer resources. Cereals are not represented in any of the samples, supporting the notion that their adoption may have been a slow process, occurring more gradually than for other domesticated foods, or that they were not initially processed in ceramic vessels.     

First Approach to Study the Presence of Domesticated Camelids (Lama glama) in the Chaco‐Santiago Region,a Marginal Zone of the South Central Andes

We analysed bone remains of camelids from the Maquijata site, assigned to the late pottery‐making and farming stage (ca. 800–400 BP), in order to differentiate between domestic and wild species in the Chaco‐Santiago archaeological region. This region is considered marginal to the Argentine Northwest regarding its cultural development and has received less attention than other regions. South American camelids have been one of the major resources in this continent, with domestication recorded in the South Central Andes at around 4400–3000 BP. We applied osteometric techniques and multivariate statistical analyses to proximal phalanges; the results showed interspecific differentiation of archaeological specimens, though with some methodological application issues. These results are the first contribution regarding the presence of domesticated animals in the study area. Copyright © 2012 John Wiley & Sons, Ltd.     

Examining dispersal mechanisms for the translocation of chicken (Gallus gallus) from Polynesia to South America

DNA sequencing and radiocarbon dates from the archaeological site of El Arenal-1 (Arauco Peninsula, south central Chile) suggest that Polynesians may have introduced chicken (Gallus gallus) to South America prehistorically (A.D. 1300–1420). The Chilean specimen identified has the exact same amplified mtDNA pattern as samples recovered from archaeological sites in Samoa and Tonga, but is only one base different from prehistoric chicken samples from Tonga (n = 1), Niue (n = 1), Easter Island (n = 1 of 6), and Hawai'i (n = 1). To examine the possible dispersal mechanisms of chicken between Polynesian islands and the Americas, we conducted computer simulations of seafaring from these six island groups and the Tuamotus, as well as return voyages. Simulation results from sailed vessels suggest that in certain months, landfall on the Pacific Coast of South America from Samoa and Tonga was fairly successful at around 40% (particularly in March for the former and January/February for the latter). For Niue, sailed vessels were 21% successful in January with only 2% success in December. Sailed vessels from the Tuamotus (a proxy for closer island groups to the west with evidence for chicken such as the Cook Islands) were successful in January and December, with 15% and 23% of the vessels making landfall in South America. Vessels sailing from all simulations heading east from Easter Island resulted in landfall. Hawai'i had only a 1% success rate if sailing in January, but high numbers of vessels reached southern Mexico and Central America in August and September. Most landfalls occurred much farther north than Chile, in Ecuador and Peru. Unsuccessful voyages from all of the islands other than Easter Island and Hawai'i often made landfall on islands to the east. Return voyages from Chile were not possible or extremely rare during most times of the year. Overall, seafaring simulations suggest that Polynesians may not have initially landed in close proximity to El Arenal-1—contemporaneous or even earlier archaeological evidence of translocated chicken remains may indeed be found further north. In addition, if Polynesians found it difficult or impossible to return to their homeland, the possibility exists that co-mingling between Polynesians and South American groups resulted in offspring that carried an admixture of genetic traits.     

Integrated seismic tomography and ground-penetrating radar (GPR) for the high-resolution study of burial mounds (tumuli)

The study primarily aims at providing adequate imaging resolution of large and prominent targets of archaeological interest, such as pyramids and tumuli, at all depth levels. We implemented an integrated seismic tomography and georadar (STG) technique to perform high-resolution imaging and characterization of tumuli (burial mounds). We tested the proposed technique on a preserved late Bronze Age burial mound in northern Italy, for which STG succeeded in performing an accurate 3-D reconstruction of the structure and stratigraphy as proved by later archaeological excavations. We completed two transmission seismic tomography measurements, at present ground level and at 1.5 m elevation, with a 24-channel seismograph and 15° angular separation between geophones. The ground-penetrating radar (GPR) dataset encompasses 12 250 MHz radial profiles and 12 common mid point gathers for velocity analysis. Shallow layers of the mound are successfully imaged by GPR, whilst the structure of the deep central part of the tomb is reconstructed from seismic traveltime inversion. In particular, GPR images lenses and layers of sediments forming the external part of the tumulus, evidences of a looting attempt, peripheral structures associated with later exploitation of the mound (furnaces) and, in the external sector of the tumulus, the top of the deep layer of silty sediments covering the funeral chamber. Tomographic results reveal seismic velocity anomalies of potential archaeological interest at ground level, which were successively validated by archaeological excavations. The integration of GPR and tomographic datasets is an effective strategy to overcome the imaging and interpretation problems related to the structure of such peculiar funeral monuments. STG can be applied to a virtually unlimited dimensional range and requires a limited data acquisition, processing and inversion effort. The results of the study allowed the identification of the funeral chamber and a detailed imaging of layering and structural details.     

圣水牛的家养/野生属性初步研究

水牛是现在中国南方稻作区的重要家畜之一。从更新世时期到全新世时期,中国南、北地区的考古遗址皆有水牛遗骸出土。本文从这些出土的水牛遗骸入手,综合其他学者的意见,对广泛出土于全新世时期遗址的圣水牛的家养/野生属性进行探讨,并提出了自己的观点。     

Starch grain analysis for groundstone tools from Neolithic Baiyinchanghan site: implications for their function in Northeast China

Large numbers of groundstone tools have been uncovered from archaeological sites in the Early Neolithic period in China. Traditionally they are often regarded as agricultural tools for processing cereals. In this paper we report preliminary starch grain analysis of groundstone tools from Baiyinchanghan site to gain a better understanding of use of these tools in the Northeast China. We found that starch grains on these groundstone tools are most likely from Quercus sp. The result of this study indicates that many groundstone tools for the Early Neolithic period were used to process wild and domesticated plants alike.     

A taphonomic study of the African wild dog (Lycaon pictus)

Carnivore taphonomy has been traditionally used for the interpretation of archaeological sites in order to discriminate human-generated or modified from non-anthropic bone assemblages. In most of this actualistic research, the focus has mainly been placed on hyenas and felids, neglecting other carnivores. This paper analyzes the taphonomic impact of the African wild dog (Lycaon pictus) on equid bones and compares it with the bone modification patterns produced by other canids, such as wolves (Canis lupus) in order to compare medium-/large-sized canid variability on bone modification patterns and elaborate a referential framework which could be feasibly applied to the zooarchaeological record to detect canid intervention on archaeological assemblages in the past.