Genetic analysis of archaeological wood remains: first results and prospects

A total of 51 ancient oak wood samples originating from various European archaeological sites, dating from the Neolithic period to the 18th century, were assayed for the presence of reproducible chloroplast (cp) DNA sequences. Five polymorphic chloroplast fragments were targeted. Only five of the samples could be fully genetically characterised, revealing four different oak cpDNA haplotypes. In all cases, the haplotypes detected on ancient woods and the haplotypes characterised from fresh samples from the same localities matched. Overall, this congruence is consistent with a genetic continuity between ancient and modern European oaks, confirming the hypothesis that the mapped genetic patterns largely reflect the original structure that established during the post-glacial. This stability of the genetic structure implies that, in the future, the technique could be used to infer or confirm the transport of wood by man, providing interesting perspectives for the genetic analysis of ancient woods.     

The wood in the pits of terracotta figures and its architectural application

Ever since the pits of terracotta warriors and horses in China were discovered and opened to the public, a number of questions remained unsolved: what kinds of wood were selected for building the pits, how the wood was used in the pits' architecture and where was the giant volume of wood collected from? In this study, twenty pieces of charcoal specimens from Pit 1 and Pit 2 were examined with the Scanning Electron Microscope and identified as Abies, Tusga, Picea (all three genera in Pinaceae) and Phoebe (Lauraceae) woods. With the former works, it is concluded that the woods in the pits were mainly from the group of gymnosperms (95%) and few from that of angiosperms (5%). It is deduced that the wood in the pits was probably collected from the areas nearby.     

Cainozoic euphorbiacean wood from the Canning Basin,Western Australia

A new species of fossil wood is described, Bridelioxylon canningense Bamford & McLoughlin, belonging to the Phyllanthoideae group of the Euphorbiaceae. The woods occur in indurated sandstones and conglomerates preserved in palaeochannels incised into the Upper Permian Condren Sandstone in the Landrigan Cliffs, northern Canning Basin, Western Australia. Based on the presence of the dicotyledonous woods and the geomorphological setting of the palaeochannel, a Palaeogene age is suggested for the host rocks. This is the first record of fossil euphorbiacean wood in Australia; pollen has been previously recorded. The fossil wood is most closely comparable to extant members of Bridelia that currently occupy the warmer and wetter parts of Australia.     

Study of interactions between polyethylene glycol and archaeological wood components by C high-resolution solid-state CP-MAS NMR

While use of polyethylene glycols, PEG, polymers for archaeological wood conservation has been well established, there is almost no study of such composite materials with carbon thirteen high-resolution solid-sate NMR. We demonstrate that NMR is an useful analytical tool to characterize rapidly samples of conserved wooden objects. By choosing an appropriate value of the contact time (from 3 to 5 ms) for which the PEG component gives only a small residual NMR signal in the CP-MAS experiment, it is possible to edit selectively the spectra of the wood components. It allows one to visualize the degradation extent of the archaeological wood even when the conservation processes have been already applied. Moreover, by studying the kinetics of CP-MAS experiments, the time constants, T_1ρH and T_CH values for PEG moiety were computed. From these data, it is shown that either in the crude commercial product or in incorporated in archaeological woods, two components are present and they are the signatures of crystalline (or ordered) and amorphous (or disordered) molecular domains. Moreover, it was shown in the archaeological woods that almost 30% of PEG was in close molecular interactions with lignins. This study is the first evidence that the PEG in conserved wood can interact at a molecular level with wood components. It also demonstrates that the PEG diffuses inside the residual cell walls of archaeological wood. This data are important for scientists in charge of process development for the conservation of wooden artifacts.     

Pollen-wood analysis at the Neapolis harbour site (1st–3rd century AD,southern Italy) and its archaeobotanical implications

Three Roman shipwrecks (Napoli A and Napoli C – 1st cent. AD; Napoli B – 2nd century AD) were recovered in the sandy-silt sediments representing the infilling of a protected inlet of Neapolis harbour (Naples, southern Italy). Extensive wood analysis suggests that a very attentive selection of species was made in shipbuilding, the choice of timber being related to wood technological properties and to the structural uses of the construction elements. Pollen data obtained from the coeval sedimentary layers revealed that all the timber taxa (apart from Picea/Larix) were present in the surroundings of the study area. The identified forest taxa are very common in the Mediterranean basin and thus the pollen-wood comparison was not able to define the location of the shipyards. Broad comparison with western Mediterranean wrecks evidenced the peculiarity of the Neapolis ships where the systematic use of both Juglans regia and Cupressus sempervirens was highlighted. Archaeological, biogeographical and archaeobotanical considerations suggest the local provenance of the ship C and constrain the possible origin area of both the ship Napoli A and Napoli B to central-southern Tyrrhenian coasts.     

First record of fossil woods from the Mariño Formation (Miocene), Mendoza,Argentina and their palaeobiogeographical implications

The first Miocene records of silicified fossil woods from the Mariño Formation, Potrerillos area, Andes Precordillera, Mendoza province, Argentina are described. Rhaphithamnoxylon artabeae gen. et sp. nov. is described as the first fossil wood referable to Verbenaceae from Argentina. This new fossil species is related to extant Rhaphithamnus Miers, sharing the following anatomical features: diffuse porosity, distinct growth ring boundaries, numerous small to very small vessels, commonly in radial multiples, 1–3 seriate rays, and heterocellular and scarce paratracheal axial parenchyma. Rhaphithamnus contains only two extant species: R. spinosus (A.L. Juss.) Moldenke, which occurs in the Valdivian forests of Chile and Argentina, and R. venustus (Philippi) Robinson, which is endemic to the Juan Fernández Islands. Representatives of Verbenaceae are distributed predominantly in the Americas from Patagonia (Argentina) to Canada, and they are inferred to have originated in South America. The fossil wood described herein provides new age and geographical constraints on the raphithanoid lineage within Verbenaceae. Other fossil woods recorded from the Mariño level are retained under open nomenclature, as they possess a combination of mostly solitary broad vessels, and smaller vessels in radial multiples or in clusters, with numerous, vasicentric to confluent axial parenchyma, and heterocellular, high rays. Thus, they have features akin to dicotyledonous lianas or vine-like or small shrub species.     

Oaks,tree-rings and wooden cultural heritage: a review of the main characteristics and applications of oak dendrochronology in Europe

We overview the recent development of oak dendrochronology in Europe related to archaeology and art-history. Tree-ring series of European oaks (Quercus robur and Q. petraea) have provided a reliable framework for chronometric dating and reconstruction of past climate and environment. To date, long oak chronologies cover almost the entire Holocene, up to 8480 BC and the network over the entire area in which the two oaks grow is being improved. We present the main characteristics of oak ring series and discuss the latest methodological advances in defining the calendar year in which the tree-rings were formed and in interpreting such dating in terms of the age of a wooden object. Dendrochronology has established itself as a standard dating tool and has been applied in a wide variety of (pre-)historical studies. Archaeological wood, historical buildings, works of art (such as panel paintings and sculptures) have been successfully investigated. Recent advances in dendro-provenancing have helped to obtain more information on the timber trade in the past. Information on past forest structures, silviculture and timber use have become available through scrutinizing historical and contemporary ring-width patterns.     

The use of forest resources in central Iberia during the Late Iron Age. Insights from the wood charcoal analysis of Pintia,a Vaccaean oppidum

Charred woods may be used to effectively reconstruct past wood acquisition strategies. We used anthracological data from the pre-Roman settlement of Pintia (Padilla de Duero, Valladolid) to examine the use of forest resources at the local scale. Palaeoecological data revealed heterogeneous landscapes in the inland northern meseta with environments that offered diverse sources of wood for the inhabitants of Pintia, one of the first cities of inland Iberia. Pines (Pinus pinea/pinaster and Pinus sylvestris/nigra) and both evergreen and deciduous Quercus L. and Juniperus L. were the main taxa identified as both fuelwood and construction elements, but the assemblages and frequencies of these taxa differed depending on their use. We also examined the potential of models from human ecology by considering the frequency, handling time and the relative technological value of each taxon to model how people gathered wood resources. The results suggest that although local availability affected the forest resources that were used by the Vaccaei people, specific taxa were positively selected for specific uses.     

Tikal timbers and temples: ancient Maya agroforestry and the end of time

Tikal, a major lowland Maya civic-ceremonial center in the heart of the Petén region of Central America, relied heavily on the adjacent lowland rainforest as a resource base for fuel and construction materials. In this study, we analyzed 135 wood samples from timbers used in the construction of all six of the city's major temples as well as two major palaces to determine which tree species were being exploited and to better understand ancient Maya agroforestry practices during the Late Classic period. We found evidence for a change in preference from the large-growing, upland forest species, Manilkara zapota, to a seasonal wetland species Haematoxylon campechianum in A.D. 741 as well as a decrease in lintel beam widths over time. Though M. zapota later returned as the wood species of choice in A.D. 810, beam widths were found to be significantly smaller. These findings concur with models that hypothesize widespread deforestation during the Late Classic period and indicate a declining forest resource base by the 9th century A.D. Because of the many large timbers available for temple construction in the 8th century, some system of forest conservation is indicated for the ancient Maya prior to the Late Classic period.     

Miocene wood from the LaTrobe Valley coal measures,Victoria, Australia

An initial study of a collection of fossil conifer wood is reported from the late early Miocene Yallourn Clays, an interseam unit intergrading into the base of the early to middle Miocene Yallourn seam of the LaTrobe Valley, Victoria in southeastern Australia. The fossil wood shares characteristics with the modem genera Dacrycarpus and Dacrydium. On the basis of contiguous, uniseriate tracheid pitting and 1–2 podocarpoid cross field pits, it is placed in the form genus Podocarpoxylon, and the new species P. latrobensis. The wood is compared with extant Podocarpaceae and other Australian fossil woods. Its ring anatomy is consistent with low temperature or rainfall seasonality in the early Miocene.     

DETERIORATION ASSESSMENT OF WATERLOGGED ARCHAEOLOGICAL LIGNOCELLULOSIC MATERIAL VIA 13C CP/MAS NMR*

This study investigates the residual chemical composition of waterlogged archaeological lignocellulosic material found in Greece. Hazelnut pericarps and oak wood found in a 16th‐century wreck, along with endocarps of olives dated at 300 bc , were examined. ¹³C CP/MAS NMR was applied to compare fresh and waterlogged archaeological materials. Results show qualitative alterations in their composition. The virtual absence of peaks at ~21 ppm and ~173 ppm, corresponding to hemicelluloses in all archaeological materials, indicates that these constituents can be an important indicator in assessing the type and degree of deterioration.     

考古木材降解评价的物理指标

为寻找一种能较为科学、方便地反映木材降解程度的指标,共收集不同地方出土的13种木材,8个树种,对这些树种的考古木材与新鲜木材的含水率、干缩性、基本密度、气干密度、综纤维素等指标进行对比分析。实验表明,基本密度和最大含水率(饱水含水率)基本上能够反映古木的降解程度,可以作为反映古木降解程度的物理评价指标。     

Wood deterioration in Chacoan great houses of the southwestern United States

Abstract

‘Great houses’ built by the Ancestral Puebloans (Anasazi) (900–1200AD) still contain thousands of pieces of wood used as beams, secondary roof supports and lintels. The wood is an integral part of the surviving Chacoan architecture and has served as a valuable resource for determining the exact age of the structures and for obtaining information about raw material production, procurement and harvesting methods. An assessment of wood deterioration at the great houses in Aztec Ruins National Monument and Pueblo Bonito, Pueblo del Arroyo and Chetro Ked located in Chaco Culture National Historic Park in New Mexico have revealed that both microbial and non-biological deterioration was taking place. The major type of decay found throughout the structures was caused by soft-rot fungi. Cavity formation in secondary walls of conifer woods and an erosion of cell walls in aspen woods was prevalent. A brown-rot type of wood degradation was also found, primarily associated with areas of subterranean termite damage. Another form of deterioration present in some of the woods was a chemical de fibration of wood. This corrosive attack, caused by high concentrations of salts, destroyed the middle lamella between cells, resulting in a stringy mass of loosely attached fibres. Reburial of great house structures as an effective conservation strategy will require an environment that is not conducive to decay, since active wood-destroying fungi are present in the prehistoric woods. If moisture and other conditions suitable for decay exist, the wood will be destroyed and this important historical resource lost.     

Charcoal in alluvium of mountain streams in the Bieszczady Mountains (Polish Carpathians) as a carrier of information on the local palaeoenvironment

Fragments of charcoal are present in floodplain alluvium of the San and Strwi?? rivers in the Polish part of the Bieszczady Mountains, Polish East Carpathians. They occur as single clasts or in lenses in the basal part of fine-grained alluvium, together with unburnt wood debris, or in the middle part of the vertical sequence of the floodplain alluvium. 14 samples of charcoal from the upper courses of the San (ca. 50 km) and the Strwi?? (ca. 10 km) were dated with radiocarbon. The obtained dates fall mainly in the 15^th through 19^th century interval; only one sample is markedly older (9^th–10^th century). Taxonomic composition of the charcoal source wood was also studied and compared with that of coeval forests. Correlation of the charcoal age with the history of economic development of the studied region indicates that charcoal is of anthropogenic origin: older charcoal from intense slash-and-burn deforestation, while younger charcoal was produced by local industries.     

Reburial of shipwrecks in marine sediments: a long-term study on wood degradation

Historical shipwrecks in marine environments are continuously decomposed by wood-degrading organisms, adapted to this specific environment. To protect the wrecks from degradation and to preserve the unique remains for future generations, reburial of wrecks using various covering materials has been suggested as a long-term preservation method. The following experiment was carried out to study the bio-protective effect of sediment. Sound oak, pine, and birch samples were buried above and within marine sediment in Marstrand harbour, and retrieved for analysis after 6, 12, 24, and 36 months. Macroscopic as well as light-microscopic examinations were carried out on each occasion. Marine borers (shipworm, Limnoria), soft rot and tunnelling bacteria were wood degraders immediately above the seabed; soft rot, tunnelling bacteria, and erosion bacteria were active 10 cm below the seabed; erosion bacteria were the only degraders at a depth of 43 cm below the seabed. The wood species had different durability towards the decay agents. After 3 years, wood samples above the seabed were totally decomposed, whereas wood was highly protected at 43 cm depth in sediment. In the sediment, decay decreased significantly with depth of burial. The results suggest that reburial of shipwrecks in marine sediment can be recommended as a simple and efficient method for long-term preservation of the wooden cultural heritage.     

Differentiating Aspen and Cottonwood in Prehistoric Wood from Chacoan Great House Ruins

Accurate taxonomic identification is an essential part of archaeological wood analysis. However, making identifications more precise than the genus level is usually not possible since species within the same genus typically possess very similar cellular morphology. This paper describes a method for distinguishing aspen (Populus tremuloides) from cottonwood (Populus fremontii, Populus angustifolia, Populus acuminata) in samples of wood collected from the San Juan Basin in northwestern New Mexico and southwestern Colorado. This method is then applied to archaeological wood samples from the Anasazi great house at Aztec Ruins National Monument in Aztec, New Mexico. The results of this study demonstrate that quantifiable differences do exist between aspen and cottonwood species and that the technique can be used to separate archaeological specimens of Populus wood.     

Stamped bricks from the ager cosanus (Orbetello,Grosseto): integrating archaeometry,archaeology, epigraphy and prosopography

This paper documents a comprehensive study of thirty-two stamped bricks found in territory of the ancient town of Cosa (Ansedonia, Grosseto). The geographical distribution of these materials distinguished ‘municipal’ productions, i.e. those found within the territory of Cosa (ager cosanus) only, and ‘urban’ productions, i.e. those found in the ager cosanus, as well as in the territory comprising Rome and its suburbs and/or the entire Tiber Valley. Main aim of the study was to verify the provenance of ‘municipal’ productions, by a combination of archaeological, epigraphical, prosopographical and archaeometrical (OM, SEM–EDS, ICP-OES, ICP-MS, INAA) research instruments and techniques. Data integration revealed that six ‘municipal’ productions (over a total of nine) were produced in the territory surrounding the Cosa's promontory, using marine and fluvial clayey deposits. Conversely, three ‘municipal’ productions were assigned to the ‘urban’ area, and possibly assigned to the Tiber Valley. An important distinction was made among the ‘urban’ productions, proving the feasibility of their discrimination.     

Timber economy in the Roman Age: charcoal data from the key site of Herculaneum (Naples,Italy)

The city of Herculaneum (Naples, southern Italy), buried by the volcanic eruption of Mount Somma-Vesuvius in 79 ad, is a key site for understanding the timber economy during the Roman period. In this paper, the results of charcoal analysis of different building element types are presented. Beams, joists, poles, planks and door and window frames were investigated allowing us a view of which timber the Romans preferred for building in this area. We also fit the taxonomic results into the reconstruction of the ancient Campanian landscape, and finally, we discuss the knowledge that the Romans had about the technological properties of the wood that they used for building and the possible selection criteria that they followed in choosing them. Coniferous timber is the preferred material for building purposes. Abies alba is especially used, this fact confirming its stronger presence in southern Italian woods during the past and suggesting that its decline is mainly due to human overexploitation. The large presence of Cupressus sempervirens, selectively used for the production of poles, confirms that this tree was cultivated in plantations for timber production in the Vesuvius area. Furthermore, it might indicate that cypress could have been present as a natural tree in the local vegetation, suggesting a forest type that nowadays almost completely disappeared from this area and from the entire Italy. The findings of Juglans regia, Pinus pinea and Olea europaea, typical elements of the Mediterranean cultural landscape, show that their use was not limited to fruit production and that Romans also appreciated their timber. Beside these local resources, the presence of Picea abies and Picea/Larix indicates the importation of timber from northerly regions, probably the northern Apennines and the Alps.     

The prehistoric pile-dwelling settlement of Stagno (Leghorn,Italy): wood and food resource exploitation

A pile-dwelling settlement, dated to the final Bronze–early Iron Age (XII–XI century BC), was excavated at Stagno, near Leghorn (Tuscany, Italy). The site presented a well-preserved portion of the wooden foundation structure buried by sediments rich in plant remains: both wood and plant remains were subjected to archaeobotanical investigation. The ultimate goal was to improve our knowledge on agricultural economy and wood usage in Tuscany during the prehistoric ages. The results pointed to a farming system based on Triticum spp. (wheat), Hordeum spp. (barley) and Leguminosae cultivation in addition to the gathering of wild fruits, such as Corylus avellana, Cornus mas, Prunus spp., Vitis spp. Many of these plant remains are associated to a wetland context. Mesohygrophilous trees, such as deciduous Quercus, Ulmus minor, Fraxinus cf. excelsior, and Sorbus were used for the construction of the pile-dwelling structures; the choice of these plants indicates a good knowledge of the technological characteristics of timber.