The antiquity of Olivella shell beads at CA-ORA-64: AMS radiocarbon dated between 9420 and 7780 cal BP

Investigations in the 1960s and 1970s showed the Irvine site (CA-ORA-64) to be among the oldest shell middens known from the Pacific Coast of North America. The site chronology, based on conventional analysis of mixed shell samples collected from heavily bioturbated soils, extended back to ca. 8440 RYBP. Recent work at the site provided an opportunity to refine the CA-ORA-64 chronology via AMS ¹⁴C dating of single shell fragments, including 14 Olivella beads. Long considered to be one of the earliest ornament types made by Pacific Coast peoples, such spire-removed Olivella beads were used through much of the Holocene and generally are not good chronological indicators. AMS analysis of the CA-ORA-64 specimens, however, produced a consistent series of Early Holocene dates that includes some of the oldest securely dated shell beads in North America. Along with obsidian from interior sources, Olivella beads played an important role in early exchange networks between coastal and interior peoples. Methodologically, our research demonstrates the utility of AMS ¹⁴C dating in determining the age of key artifact types found in multicomponent sites with assemblages affected by stratigraphic mixing.     

Sources of Archaeological Obsidian on Sakhalin Island (Russian Far East)

Instrumental neutron activation analysis was performed on 79 obsidian tools and flakes from 35 sites on Sakhalin Island dating from Upper Paleolithic (c. 19,000 bp ) to Early Iron Age (c. 2000–800 bp ). Due to the absence of volcanic glass on Sakhalin Island, raw materials from the nearest obsidian sources on Hokkaido Island, such as Oketo, Shirataki, Tokachi-Mitsumata, and Akaigawa, were also analysed. A strong correlation between the chemical compositions of obsidian artefacts from Sakhalin and volcanic glass sources from Hokkaido was discovered. In particular, the Oketo and Shirataki sources were used for tool manufacturing throughout all of Sakhalin Island's prehistory. The distances between sources and archaeological sites range from 200–1000 km. The intensive exchange of raw materials continued and even intensified after the appearance of the La Pérouse (Soya) Strait between Hokkaido and Sakhalin about 10,000–8000 bp. The Sakhalin Island populations were deeply involved in the obsidian exchange network centered on Hokkaido.     

Carbon isotope fractionation between diet,breath CO2, and bioapatite in different mammals

The carbon isotope fractionation between tooth enamel bioapatite, breath CO₂, and diet was measured for voles, rabbits, pigs, and cattle on controlled diets. The measured fractionation (expressed as isotope enrichment ɛ*) between enamel and diet was 11.5 ± 0.3‰, 12.8 ± 0.7‰, 13.3 ± 0.3‰, and 14.6 ± 0.3‰ for these respective species. There is a 1:1 correlation between ɛ*_{breath–diet} and ɛ*_{enamel–diet} (r² = 0.94, p < 0.01), whereas our data do not resolve significant inter-species differences in ɛ*_{enamel–breath}. These findings are consistent with the hypothesis that inter-species differences in ɛ*_{bioapatite–diet} are primarily a result of differences in digestive physiology, rather than differences in the magnitude of fractionation between mineral and body fluid.     

Radiocarbon dating and the “old shell” problem: direct dating of artifacts and cultural chronologies in coastal and other aquatic regions

Archaeologists have long recognized the problem of the “old wood” effect in radiocarbon dating charcoal and wood samples, the age of which may be hundreds of years older than their use by humans. Such problems have resulted in significant changes in how most researchers select wood and charcoal samples for ¹⁴C dating, with many now using relatively short-lived carbonized materials for dating. Despite the significant strides made in our understanding of the potential biases of the “old wood” effect, little emphasis has been placed on the possible impacts of dating “old shell” in archaeological deposits. The use of marine shell for ¹⁴C dating is widespread in coastal areas around the world, including a growing emphasis on the dating of individual shell artifacts via Accelerator Mass Spectrometry (AMS). In dating shell artifacts, we have obtained several dates older than associated ¹⁴C dates for short-lived subsistence remains from the same deposits, including great disparities (>10,000 years) and more subtle differences (≥100 years). These discrepancies appear to be due to the use of old shells by humans to make beads and other artifacts, including shells collected from fossil deposits, older archaeological sites, and beaches. The problems caused by the use of old shells to make beads and other artifacts are surmountable through careful sample selection, analysis of multiple ¹⁴C dates on a variety of materials, and proper calibration procedures.     

Reconstructing northern Chinese Neolithic subsistence practices by isotopic analysis

Stable isotope signatures of both human and non-human animal bone samples indicate that Neolithic farmers of the Yellow and Wei River basins in China potentially cultivated millet for two reasons: as a staple for human consumption and as fodder for domesticated animals, specifically pigs, dogs, and perhaps chicken. Bone samples were analyzed from four Neolithic sites: Jiangzhai, Shijia, Xipo, and Kangjia, spanning the time period from 7000 to 4000 years ago. A combination of very high carbon isotope ratios (δ¹³C = −7.7 ± 0.4‰) and low nitrogen isotope ratios (δ¹⁵N = 7.5 ± 0.5‰) in samples of Xipo pig and dog bone suggests that these monogastric animals consumed substantial quantities of C₄ plants, almost certainly millets. In fact, the proportion of C₄ plants in animal diets appears to have been even greater than that in human diet. Stable isotope values (δ¹³C = −10.0 ± 0.8‰; δ¹⁵N = 8.3 ± 0.5‰) of human bone collagen recovered at Jiangzhai and Shijia indicate a staple role for millets, as well as the consumption of both wild and other non-C₄ domesticated plant foods. As millet agriculture and animal husbandry apparently depended on one another, a strong mutualism between them was likely established in northern China during the Neolithic. We propose that variable redistribution of agricultural products between humans and animals, depending on the availability of wild resources and annual fluctuations in agricultural output, helped ensure the stability of Neolithic human subsistence in the Yellow and Wei River basins.     

Analyzing the process of domestication: Hagoshrim as a case study

Recent excavations at the Neolithic site of Hagoshrim, northern Israel, have yielded a large assemblage of skeletal fragments, representing mostly caprines, cattle and pigs. The three layers of the site's occupation span approximately 2000 years of a crucial period in the domestication of these taxa in the southern Levant, including Pre-Pottery Neolithic C (Layer 6, 7562 ± 85 BP and 7735 ± 55 BP) and the Pottery Neolithic cultures, Jericho IX (Layer 5, 6725 ± 120 BP) and Wadi Raba (Layer 4, 6505 ± 120 BP). Therefore, this site provides an outstanding opportunity to study the process of domestication in a comparative manner, both across taxa and through time. We used kill-off patterns, size reduction and changes in body proportions, and introduced statistical methods to discern the different stages of the domestication process for each taxon. Pig remains reflect simultaneous changes at the end of the 7th millennium BP: kill-off patterns, size and proportions of cranial and post-cranial elements all change between Layer 5 and 4 with no significant changes between Layer 6 and 5. Gradual changes—both between Layer 6 and 5 and between Layer 5 and 4—were found only for cattle, while caprine remains exhibit no changes throughout the site's occupation. These results can be explained in light of the differences between the taxa in terms of their life history strategies, among other things, that can be viewed as pre-adaptations to domestication.     

A rock magnetic record of Pleistocene rainfall variations at the Palaeolithic site of Attirampakkam,Southeastern India

Attirampakkam, an open-air stratified Palaeolithic site in southeastern India is the focus of ongoing studies to investigate the nature of Lower and Middle Palaeolithic occupation in relation to changing Pleistocene environments. The paucity of faunal or palaeobotanical remains at the site required the use of rock magnetic properties (χ_lf, χ_fd, χ_fd %, χ_ARM, SIRM, χ_ARM/SIRM, SIRM/χ_lf, χ_ARM/χ_lf, χ_ARM/χ_fd, S-ratio, HIRM and HIRM/IRM_300mT) as proxies for deciphering the palaeoenvironments and palaeoclimates of the region. Rock magnetic data from stratified deposits within excavated trenches show that the bulk of the sedimentation was intermittent with no subaerial exposure of sediments for long periods, and points to fluctuating wetter and drier climates associated with the Acheulian to Late Middle Palaeolithic levels. There is a general trend towards a wetter climate from Layer 5 upwards. Layer 7 offers evidence of bacterial magnetite (χ_ARM/χ_lf > 40 and χ_ARM/χ_fd > 1000), suggesting the onset of micro-anaerobic environments as a result of floods. Greigite is indicated in Layer 5 (SIRM/χ_lf > 30 × 10³ A m^?1), which indicates a reducing environment. Layer 2 represents an arid climate (higher proportion of haematite and a coarser magnetic grain size). Layer 1a again is indicative of an arid climate (presence of calcrete, magnetically “hard” minerals and coarser magnetic grain size). Results indicate that the southeast coast of India experienced a mostly dry tropical climate during the Pleistocene. The detected occurrence of a few notably more arid intervals did not disrupt occupation of the site by successive groups of hominins.     

Palaeogeographical reconstructions of Lake Maliq (Korça Basin,Albania) between 14,000 BP and 2000 BP

Since the early 1990s, excavations of a protohistoric lakeside settlement in the Korça basin carried out by a French–Albanian archaeological team have induced geomorphological and palynological studies about the sedimentary records of Lake Maliq. These studies allow us to distinguish a series of centennial-scale high and low lake level events between 4200 and 4000 cal BP (2899–2637 BC/2843–2416 BC) and 2600 cal BP (822–671 BC), probably due to large-scale climate changes (in the Mediterranean basin). In addition, the sediment sequence also gives evidence of a millennial-scale trend of lake level rise. It appears to be an interplay between lake level rises and falls against tectonic subsidence of the basin allowing accommodation space for sediment deposition.The variations of the lake's level and the lake's surface area influenced the development and the abandonment of the nearby lakeside settlements (like the tell of Sovjan). In order to prepare an archaeological survey around the now dried up lake, we made a 3D model of the Holocene deposit from the lake including these lake level results, geomorphological mapping, excavation data, numerous core logs, AMS ¹⁴C dating and SRTM DEM data. The GIS model allowed us to propose four palaeogeographical reconstructions of the extension of Lake Maliq: around 14,000 BP, during the Mesolithic (around 9000 BP – 8781–8542 BC), the Early/Middle Bronze Age transition (around 3800 BP – 2310–2042 BC) and the Iron Age (2600 BP – 822–671 BC). A map of the thickness of the sediments above potential archaeological layers is also proposed.     

Land Snails from an Ancient Shipwreck: The Need to Detect Wreck-Independent Finds in Excavation Analysis

Land snails recovered from shipwreck excavations can potentially provide information regarding human-based dispersal of the involved species and also contribute to hypotheses regarding a ship's route and geographical origins of some of its cargo. Such faunal material, however, must be subjected to critical study to ascertain whether they represent specimens originally associated with the ship itself or are simply elements introduced to the site after the ship sank. The excavation of a Late Bronze Age shipwreck at Uluburun, in southern Turkey, produced 36 land snails. Of these, 32 specimens are believed to have been on board the vessel in antiquity. Three other specimens represent an endemic Metafruticicola species, which lives exclusively in a 10 km zone in the region of Uluburun. The proximity of the species' habitat to the shipwreck site suggests that these specimens are intrusive elements. The intrusive nature of a single Zonites specimen also recovered from the excavation is amply demonstrated by a detailed comparative study of Zonites specimens collected in the same locality. The last study, which involves the analysis of spatial shell variations of populations collected from 61 separate localities (totalling 367 specimens) within a 50 km area extending from Megisti (Kastelórizo) and Kas to Finike, suggests that: (1) Zonites beydaglariensis is conspecific with Z. caricus, and (2) the shipwreck specimen not only belongs to this species, but that it originates from a population on the rocky Uluburun peninsula 0·8–1·2 km north of the shipwreck site. The specimen was probably blown into the sea by a natural phenomenon and settled on the shipwreck site.     

Strontium isotope analysis to reveal migration in relation to climate change and ritual tooth ablation of Jomon skeletal remains from western Japan

A gradual population increase accompanying climate cooling has been evinced as having occurred in western Japan during the Middle (ca. 5000–4000 years BP) to Late-Final Jomon period (ca. 4000–2300 years BP). We test the hypothesis that this population change paralleled increasing human migration. We also test the archaeological hypothesis that types of ritual tooth ablation can be used to distinguish between locals and immigrants during the Late-Final Jomon period. We measured strontium isotope ratios in human skeletal remains from the Middle Jomon Ota and the Late-Final Jomon Tsukumo sites located in the Sanyo region of western Japan. Tooth enamel and bone were analyzed, and modern plant samples were collected in the areas surrounding the two sites to make a map of environmental strontium isotope ratios. The biosphere strontium isotope ratios correlated well with the underlying geology, enabling us to put forth a hypothesis of immigrants’ origins. There were no migration pattern differences between the Middle and Late-Final Jomon groups, indicating that the gradual population increase was caused by an increase in the indigenous population. All the Tsukumo individuals are locals, and this finding indicates that types of tooth ablation did not distinguish between locals and immigrants. Alternative hypotheses for the presence of different tooth ablation types in the Jomon society should be explored.     

Man and woolly mammoth at the Kraków Spadzista Street (B) – taphonomy of the site

Excavations at the Kraków Spadzista Street (B) site, Poland were conducted over 16 years in which approximately 150 m² of the site was studied. The radiocarbon dates clustered around 23–24 ky BP, placing the site in the Gravettian cultural complex. In this paper, results from a detailed taphonomic study of faunal remains are discussed in conjunction with previous analyses of stone artefacts and site structure in order to address questions regarding site formation and Gravettian occupation. Up to 1994, ∼9000 bone remains were recovered and it was possible to identify the species and skeletal element for 5860 remains. The site contained bones of seven animal species associated with Pleistocene steppe–tundra habitats. At Kraków Spadzista Street (B), 99% of the faunal remains belong to the woolly mammoth, with other large mammal taxa represented by only isolated bones and teeth. This site yielded the largest number of mammoth bones and most individuals (MNI = 86) ever found in one place in Poland. Kraków Spadzista Street (B) represents a mammoth butchering locality and probably a mammoth hunting site as well. However, it is not yet possible to distinguish the mammoths killed by Gravettian hunters from those that died naturally and then were scavenged by people.     

Luminescence Dating in Less Than Ideal Conditions: Case Studies from Klasies River Main Site and Duinefontein,South Africa

Luminescence dating results are reported for two South African archaeological sites where changes in the dose rate through time are apparent. A subtraction procedure involving analysis of both quartz and potassium-feldspar extracts is applied to detect and correct for these changes. At the Middle Stone Age site of Klasies River, the dose rate changes inferred from geologic evidence for shell dissolution turn out not to be significant, having occurred rapidly after deposition. The dates here range from 50–115 ka, in broad agreement with other evidence. The important Howiesons Poort component here appears to date somewhat later (about 55–60 ka) than at several other sites in South Africa. At the Acheulian site of Duinefontein, the dose rate changes, also thought to be caused by shell dissolution, are, on the other hand, very significant. The subtraction ages here (125–300 ka) agree with independent chronological assessments.     

Assessment of material characteristics of ancient concretes,Grande Aula,Markets of Trajan,Rome

The Grande Aula, or Great Hall, of the Markets of Trajan (AD 96 to 115) is an intact example of the domed, concrete architecture of imperial Rome. Petrographic, x-ray diffraction, chemical, and SEM analyses demonstrate that wall mortars contain Pozzolane Rosse volcanic ash aggregate (harenae fossiciae) and strätlingite, a complex calcium aluminate cement hydrate (C₂ ASH₈) that gives modern cements good durability and compressive strength. Specific gravity tests and a new petrographic method for assessing bulk densities indicate unit weights of about 1750 kg/m³ for the wall mortars and 1430–1640 kg/m³ for the pumice bearing, vaulted ceiling mortars. Innovative point load source tests record the tensile strengths (f_t) of the aggregate and interfacial elements of the conglomeratic concrete fabric. These suggest f_t of about 2.7 MPa for brick, 1.2 MPa for Tufo Lionato tuff, and 0.9 MPa for Tufo Giallo della Via Tiberina tuff coarse aggregate (caementa), based on a tentative, approximate correlation with splitting (Brazilian) tests. The pozzolanic mortar and interfacial zones have lower f_t in the range of 0.8 MPa to 0.5 MPa. The relatively low mortar strength and its somewhat tenuous adhesion to the coarse aggregate suggests that the caementa may have arrested the propagation of tensile microcracks that formed in the mortar, thereby increasing the composite tensile strength of the concrete. Roman builders selected the complex aggregate mixes to optimize the performance of the wall and vault concretes.     

Seasonal stability in Late Holocene shellfish harvesting on the central California coast

Oxygen isotope determinations from 92 California mussel (Mytilus californianus) shells from ten archaeological sites in central coastal California show relatively stable seasonal harvesting patterns between 3600 CAL BP and historic contact (AD 1769). Coastal occupants harvested mussels nearly year-round and seem to have occupied individual residential bases throughout the seasonal cycle. Interior groups returned with mussels from the coast mostly in the spring and early summer, but almost never in the late summer/early fall when nut crops were harvested. These findings suggest two inter-dependent groups with distinct seasonal settlement strategies: inland people, reliant on acorns and other nut crops harvested in the fall, and coastal inhabitants who were less involved with acorns. This pattern is supported by accounts recorded by the first Spanish explorers in AD 1769. While some interior groups may have been seasonally migrating “collectors,” coastal populations were less mobile, inhabiting individual residential sites throughout the year, albeit not necessarily on a permanent basis. These findings highlight the strong influence of coastal environments and resources on hunter-gatherer mobility.     

Methods for inferring paleohabitats from the functional morphology of bovid phalanges

The functional morphology of postcranial remains can be used to infer habitat preference. This approach is typically considered an “ecomorphological” method, and has frequently been applied to bovid postcranial remains. Methods for predicting habitat preference from bovid postcrania currently exist for femora, metapodials, and astragali. Here we describe three methods for predicting paleohabitats using measurements of African bovid phalanges (proximal, intermediate, and distal). The proximal phalanx method correctly predicted the habitat preference for 130 of 183 modern bovid specimens (71.0%, 2.8 times better than chance, p < 0.0001). The intermediate phalanx method correctly predicted the habitat preference for 115 of 163 modern bovid specimens (70.6%, 2.8 times better than chance, p < 0.0001). The distal phalanx method correctly predicted the habitat preference for 87 of 122 modern bovid specimens (71.3%, 2.8 times better than chance, p < 0.0001). These accuracies compare well with those of existing such methods (1.8–3.4 times better than chance). Analysis of the probabilities associated with the habitat predictions allows confidence thresholds to be established that identify specific predictions which have <5% chance of being in error. This raises the effective accuracy of the methods to 95%. Extensive exploration and manipulation of the underlying data demonstrate that the habitat predictions are generally robust, and are relatively independent of body weight, taxonomy, and sample composition. These methods are broadly applicable, relatively accurate, and can be used to generate independent predictions of habitat from different elements, and thus constitute a useful approach to inferring past environments.     

Evaluation of radiocarbon dates from the Middle Holocene hunter-gatherer cemetery Khuzhir-Nuge XIV,Lake Baikal,Siberia

A data set of 87 radiocarbon determinations obtained for the Bronze Age Khuzhir-Nuge XIV cemetery in the Cis-Baikal region of Siberia is analyzed from the perspective of data quality and within the local archaeological context. Bone preservation, expressed in terms of collagen yields, is a very important factor affecting both the accuracy and precision of ¹⁴C dates and, therefore, publication of this information should be adopted as a required standard. According to the calibrated high-collagen dates, after a single Serovo interment the cemetery was used continuously by Glazkovo peoples for up to 700 years (∼2700–2000 BC), and 70% of all burials were interred within a relatively short peak period between approximately 2500 and 2300 BC. The extensive radiocarbon data from KN XIV allow for the re-evaluation of existing models and perspectives on the place of the Glazkovo culture within the Cis-Baikal Neolithic and Bronze Age.     

Recovery of Protein and DNA Trapped in Stone Tool Microcracks

Microcracks produced during the manufacture of stone tools may harbour ancient biological residues. Our studies test this hypothesis using light, scanning electron, fluorescence, and confocal microscopy to characterize microcracks produced in obsidian microblades by pressure flaking. Cell-sized fluorescent latex beads penetrated microcracks to depths exceeding 50 μm below the microblade surface. Fluorescently labelled blood protein and DNA were trapped in microcracks within 5 min of exposure. Washing artifacts with water did not remove blood residues from microcracks. However, 60–80% of trapped blood protein and DNA were released by an 18-hour incubation in 4M guanidine hydrochloride or by sonication in 5% ammonium hydroxide. These experiments suggest that residues trapped in artifact microcracks represent an important and often overlooked source of ancient DNA and protein.     

Selective hunting of pine marten,Martes martes,in Late Mesolithic Denmark

Pine marten, Martes martes, is the dominating fur prey at three contemporaneous Danish Late Atlantic sites, Agernæs (EMNI = 34), Ringkloster (EMNI = 41) and Tybrind Vig (EMNI = 26). All skeletal elements are represented and the only marks present on the skeleton are those consistent with skinning. Burned teeth in the three populations suggest either new hunting methods or a ritual act. Ringkloster is on the mainland, while Tybrind Vig and Agernæs are island localities. Morphometric data indicate that the subfossil population of larger individuals was maintained on the mainland due to free migration, while the animals in the subfossil island populations had decreased in size in the Late Atlantic.     

Infra-red Photoacoustic and Secondary Ion Mass Spectrometry Measurements of Obsidian Hydration Rims

A procedure has been developed for measuring obsidian hydration rim thicknesses on archaeological artefacts using infra-red photoacoustic spectroscopy (IR-PAS). Calibration of the IR-PAS values with depth was completed using secondary ion mass spectrometry (SIMS) and optical microscopy to relate the quantity of diffused water to the measured thickness of the hydration layer. By monitoring the absorbance intensity at 1630 cm⁻¹wavenumbers, hydration rims between approximately 1 μm and 12 μm may be accurately measured and used for chronometric age estimates. SIMS depth profiling has the ability to measure layers less than 1 μm thick, extending the age calibration to samples of less than 100 years in age.