Chronologies in wood and resin: AMS C dating of pre-Hispanic Caribbean wood sculpture

This paper establishes a chronological framework for selected pieces of Caribbean (Taíno/Lucayan) wooden sculpture, enabling previously ahistoric artefacts to fit back into the wider corpus of pre-colonial material culture. Seventy-two ¹⁴C AMS determinations from 56 artefacts held in museum collections are reported, including 32 ceremonial duhos, or seats. Far from being constrained to the last few centuries prior to contact, the dates for these objects extend back to ca. AD 250, and include the artistic legacies of various cultures. Duhos in both low and high back styles are present from about AD 600, if not earlier, in a distribution that spans the Antillean island chain from Trinidad to Cuba. Complex, drug-related paraphernalia and elaborate ancestral reliquaries are in evidence by AD 1000, as are some distinctive regional styles – such as the unique iconography from the Bahamas and Turks and Caicos islands. This paper explores relevant methodological issues – from the challenges of working with museum pieces (e.g., uncertain provenance, discrete sampling techniques, impact of previous conservation treatments on dating results), to dealing with potential ‘in-built’ age in tropical hardwoods.     

Birdmen, cemís and duhos: material studies and AMS C dating of Pre-Hispanic Caribbean wood sculptures in the British Museum

This paper presents 19 AMS radiocarbon dates from nine pre-Hispanic Caribbean (Taíno/Lucayan) wooden sculptures in the British Museum collections, provenanced to Jamaica, Hispaniola and the Bahamas. Together with strontium isotope results and wood and resin identifications, these data build a material and chronological context for some of the most recognised examples of Taíno art – from duhos (ceremonial seats) and cemís (free standing depictions of deities, ancestors and spirits) to canopied stands used to hold hallucinogenic drugs during the cohoba ceremony. Each sculpture widens our understanding of Caribbean carving traditions, stylistic variation, chronologies and material resource utilisation. A group of three sculptures recovered from Carpenters Mountains, Jamaica, carved by AD 1300 and brought together as a ceremonial ‘set’, each appear to have had their inlays renewed over a century later, suggesting long-term use. Three key examples of the main Caribbean duho categories (high-back, low-back and extended), provide insights into the diversity of styles present in the region post-AD 1100. The British Museum's corpus enables an exploration of regional styles, and potentially the work of individual artists.     

Metals,microanalysis and meaning: a study of metal objects excavated from the indigenous cemetery of El Chorro de Maíta,Cuba

This is the first publication reporting systematic analytical research conducted on archaeological metals from Cuba. The main focus of the study consists of beads and small metal objects excavated at the cemetery of El Chorro de Maíta, which comprises some of the richest funerary deposits so far recovered on the island. Some comparative samples from the nearby site of Alcalá were also investigated, with an emphasis made on the manufacture, composition and origins of the different alloys. The resulting picture is that members of the social elite of the indigenous Taíno peoples were buried with beads made of placer gold exploited locally, gold–copper–silver pendants brought from continental South America and, above all, brass lacetags from European clothing that were perceived as sacred metals. The archaeometallurgical approach offers fresh insight into the relationships between Europeans and Taínos, and the impact of colonization on the indigenous customs, values and social structures.     

Two cases of leprosy from Žatec (Bohemia), dated to the turn of the 12th century and confirmed by DNA analysis for Mycobacterium leprae

The leprosy known today primarily from tropical areas was a relatively common disease in European Middle Ages. This article describes two skeletons bearing palaeopathological indications of leprosy from a cemetery at ?atec in North-West Bohemia (Czech Republic). The archaeological context clearly shows that these individuals were buried prior to the second decade of the 12th century, and most probably in the second half of 11th century. This rules out the possibility that these individuals might have contracted the disease in connection with the Crusades, in which a Bohemian contingent under Prince Vladislav II participated from 1141 to 1142. Molecular genetic methods were applied to detect specific DNA fragments of the causative agent of leprosy, Mycobacterium leprae. The nasal concha of one individual yielded DNA that could be directly sequenced after isolation and amplification. The vertebral body of the second individual, on the other hand, did not provide DNA of sufficient quality for direct sequencing and only weak amplification was detectable. The morphological and genetic analyses both indicate that leprosy existed prior to the Crusades in medieval Bohemia, albeit that its prevalence was probably not as great as in northern or western Europe.     

High-throughput mass spectrometric analysis of 1400-year-old mycolic acids as biomarkers for ancient tuberculosis infection

The cell wall of mycobacteria includes an unusual outer membrane of extremely low permeability. This cell envelope consists of a characteristic cell wall skeleton, a mycoloyl arabinogalactan peptidoglycan complex, and related hydrophobic components that contribute to the cell surface properties. In this study 1400-year-old mycolic acids as unique tuberculosis biomarkers have been extracted and identified for the first time by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) and Fourier transform infrared spectroscopy (FT-IR). The data suggest that the MALDI TOF MS has potential as a rapid and reproducible technique for the detection and identification of ancient mycobacterial infections.     

Essentials in the use of mycolic acid biomarkers for tuberculosis detection: response to “High-throughput mass spectrometric analysis of 1400-year-old mycolic acids as biomarkers for ancient tuberculosis infection” by

High molecular weight long-chain mycolic acids are key structural components of the cell envelope of Mycobacterium tuberculosis and they are established as biomarkers for the identification of both ancient and modern tuberculosis. Mycolic acids from M. tuberculosis have a characteristic profile, reflecting contributions from five major distinct homologous series of mycolate structural types. Diagnosis of tuberculosis in archaeological material, using mycolic acid biomarkers, depends on objective recognition of the key characteristic mycolic acid components. A recent article in this journal claimed that tuberculosis could be confirmed in ancient bones by high throughput mass spectrometric analysis of mycolic acids. Scrutiny of the data presented reveals no convincing evidence for the presence of mycolic acids, characteristic of the M. tuberculosis complex, in the skeletal remains examined. This communication reviews the essential criteria necessary for positive tuberculosis diagnosis, using mycolic acids.     

Palaeolithic dog skulls at the Gravettian P?edmostí site, the Czech Republic

Whether or not the wolf was domesticated during the early Upper Palaeolithic remains a controversial issue. We carried out detailed analyses of the skull material from the Gravettian P?edmostí site, Czech Republic, to investigate the issue. Three complete skulls from P?edmostí were identified as Palaeolithic dogs, characterized by short skull lengths, short snouts, and wide palates and braincases relative to wolves. One complete skull could be assigned to the group of Pleistocene wolves. Three other skulls could not be assigned to a reference group; these might be remains from hybrids or captive wolves. Modifications by humans of the skull and canine remains from the large canids of P?edmostí indicate a specific relationship between humans and large canids.     

What has Iberia to do with Jerusalem? Crusade and the Spanish route to the Holy Land in the twelfth century

In spite of repeated papal injunctions forbidding them to abandon their homeland, Iberian Christians, like their co-religionists throughout Europe, were energised by a desire to participate in the Holy Land crusades. The most significant and creative attempt in the first half of the twelfth century to respond to this Spanish desire was the development of the idea of the iter per Hispaniam, which was fostered by Iberian archbishops and monarchs such as Diego Gelmírez of Santiago de Compostela and Alfonso I of Aragon. This Spanish route was intended to unite the conflicts in the peninsula and the Holy Land, forming a single Mediterranean-wide crusading theatre and thereby granting the Iberian conflict a deeper connection with the struggle for control of the holiest sites in Christendom. This article explores the development of crusading ideology in Spain and the difficulties of doing so in a venue outside of the Holy Land. Additionally, the development of the Spanish route idea provides a unique opportunity to contextualise recent historiographical discussions about the essence of the crusades and to highlight the way in which different perspectives helpfully offer insight into various aspects of peninsular crusading.     

Intensifying collection and size increase of the tessellated nerite snail (Nerita tessellata) at the Coconut Walk site,Nevis, northern Lesser Antilles,AD 890–1440

Recent archaeological investigation at the Coconut Walk site on the Caribbean island of Nevis revealed dense 40 cm deep midden deposits that accumulated between cal AD 890–1440. Analysis of invertebrate faunal remains reveals an assemblage dominated by nerite snails. We measured the length and width of more than 2700 tessellated nerite (Nerita tessellata) shells to investigate evidence for changing mean size that might be indicative of intensifying human predation pressure or other cultural and natural processes. Contrary to similar archaeomalacological studies in which size decline is detected, we observed a statistically significant size increase for N. tessellata over time. This size increase is coupled with increasing levels of tessellated nerite exploitation at the Coconut Walk site. Results suggest that tessellated nerite use was sustainable over several centuries of site occupation. Our findings have important implications for investigations of anthropogenic impacts on prehistoric mollusc populations. In addition, the findings reported here provide important insight into human subsistence patterns during the Late Ceramic Age in the Caribbean and a framework for comparison with observations from other Pre-Columbian sites in the Caribbean.     

Animal DNA in PCR reagents plagues ancient DNA research

Molecular archaeology brings the tools of molecular biology to bear on fundamental questions in archaeology, anthropology, evolution, and ecology. Ancient DNA research is becoming widespread as evolutionary biologists and archaeologists discover the power of the polymerase chain reaction (PCR) to amplify DNA from ancient plant and animal remains. However, the extraordinary susceptibility of PCR to contamination by extraneous DNA is not widely appreciated. We report the independent observation of DNA from domestic animals in PCR reagents and ancient samples in four separate laboratories. Since PCR conditions used in ancient DNA analyses are extremely sensitive, very low concentrations of contaminating DNA can cause false positives. Previously unidentified animal DNA in reagents can confound ancient DNA research on certain domestic animals, especially cows, pigs, and chickens.     

Plant microfossil analysis reveals disturbed forest and a mixed-crop,dryland production system at Te Niu,Easter Island

Plant microfossil analysis was carried out on 12 soil samples from a variety of landscape features at three locations (100–250 m a.s.l.) along a 950 m transect at Te Niu, Easter Island. Pollen and phytolith assemblages were dominated by palms and ground ferns, and suggested disturbed forest. We identified pollen of bottle gourd (Lagenaria siceraria) and starch grains of the common yam (Dioscorea alata), sweet potato (Ipomoea batatas) and taro (Colocasia esculenta). The data suggest a mixed-crop, dryland production system at Te Niu dominated by yam and sweet potato, and supplemented by taro and bottle gourd. The data provide direct evidence of crop type and range, supporting the indirect evidence (topographic and landscape features, field and historical research, comparisons with elsewhere in the Pacific) that much of the rock covered landscape of Easter Island was used for intensive horticulture.     

Provenance of marble sculptures and artifacts from the so-called Canopus and other buildings of “Villa Adriana” (Hadrian's villa – Tivoli,Italy)

The results of a minero-petrographic and isotopic study carried out on the marbles of statues and architectural elements belonging to several buildings on the “Hadrian's Villa” site (the so-called Canopus, the Peschiera, the Sala dei Pilastri Dorici and the Serapeum) are presented here as part of an ongoing archaeometric project which considers the determination of the provenance of all the marble decorations of the Villa complex. Analytical data indicate the prevailing presence of marmor lunense and the use of Pentelic and Thasian marbles for architectural elements in a few parts of the Sala dei Pilastri Dorici, the Peschiera and the Serapeum; no significant use is made of Proconnesian marble, probably the most inexpensive of the time. Analysis of sculptures from the Canopus showed that those of the Tiber and Nile gods were made of Parian marble, the Caryatids and Telamons of Pentelic marble and the Amazons of the precious white Dokymaean marble.     

Clarifying prehistoric parasitism from a complementary morphological and molecular approach

This paper reports an approach to the identification of prehistoric parasitic infection, which integrates traditional morphological methods with molecular methods. The approach includes the strengths of each method while mitigating the limitations. Demonstrating the efficacy of this approach, we provide a case study from a 1400 year old desiccated fecal sample from La Cueva de los Muertos Chiquitos, archaeological site, near Rio Zape, Durango, Mexico. Traditionally prepared microscope slides were processed via microscopy and tentative ascarids were identified. Information regarding the parasites' developmental stage was recorded. DNA was then extracted directly from the slide material. From this DNA extract, a small segment of the 18S ribosomal RNA gene variant that is specific to Ascaris, and its phylogenetically close relatives, was targeted for PCR amplification and sequencing. Phylogenetic analysis of the DNA sequence best matched a member of physalopterids, rather than ascarids, with a single exception of a match to Contracaecum spiculigerum. Subsequent extractions, amplifications and sequencing of the original rehydrated coprolite material confirmed these results. The C. spiculigerum sequence represented a phylogenetic anomaly and subsequent analysis determined the sequence was an error in the BLAST database, likely attributable to misidentification of juvenile specimens prior to sequencing and submission. Physaloptera are a difficult genus to identify morphologically and can carry major health burdens. They may be underreported in humans, in part, because of morphological similarities to the more common human parasites belonging to ascarids. We conclude that integrating traditional morphological methods with molecular methods can help resolve this issue, in both contemporary and prehistoric populations.     

Identifying donkey domestication through changes in cross-sectional geometry of long bones

The initial phases of donkey domestication are difficult to identify in the archaeological record due to late and inconsistent changes in morphology and body size in the earliest domestics. Use of donkeys for load carrying and the management of captive herds resulted in a distinctive behavioral shift away from the free-ranging speed and mobility characteristic of wild asses toward slower, more steady pacing. Given the ability of bone to adapt to its mechanical environment, bone remodeling in the limbs of wild asses and donkeys are evaluated using cross-sectional geometry to determine whether weight bearing or locomotor differences between the wild and domestic forms may be used to recognize early domestication. Cross-sectional data were collected on the humeri, radii, metacarpals and metatarsals of eight wild ass and six donkey skeletons. Wild ass forelimbs have greater overall strength and more cylindrical humeral diaphyses, indicating better resistance to a varied locomotor repertoire. These results demonstrate that analyses of shaft geometry can provide information on changes in locomotor behavior during domestication. Our finding that shifts in gait had greater effects on the morphology of early domestic donkeys than did load-carrying places a new emphasis on understanding selection for gait changes in domestication dynamics.     

New insights into the consumption of maize and other food plants in the pre-Columbian Caribbean from starch grains trapped in human dental calculus

In a first region wide study, starch grains from human dental calculus from the pre-Columbian insular Caribbean (dating to ca. 350 B.C.–A.D. 1600) are used to identify important plant foods in the diet and to assess potential dietary differences related to age or sex. Results give important insights into pre-Columbian maize (Zea mays) consumption throughout the region, confirming recent studies that indicate that maize was more commonly consumed in the insular Caribbean than originally thought. No age or sex based differences in maize consumption were found. Furthermore, based on the results of new experiments regarding grinding and pressure damage to starch grains, it is clear that maize in the Caribbean was ground, baked and consumed as bread as was the case in large parts of the mainland. Based on our results we tentatively suggest maize consumption in the Caribbean was at least in some cases associated with feasting and ceremonial activities. The variety in other plant foods identified (mostly tuberous root crops) shows that the pre-Columbian inhabitants of the region consumed a broad spectrum, but locally variable diet in which a variety of root crops functioned as staple crops, including marunguey (Zamia sp.) and sweet potato (Ipomoea batatas). We found no indications for the traditionally assumed heavy reliance on manioc (Manihot esculenta) cultivation in the region.     

Seasonality of human site occupation based on stable oxygen isotope ratios of cod otoliths

Oxygen isotope ratios (δ¹⁸O) were measured in micromilled samples of cod otoliths recovered from two Stone Age sites, one from Skoklefald, southeastern Norway and five from Skipshelleren, southwestern Norway. Discrete samples of otolith material were milled from the edge of the otolith towards the centre, each sample representing 3–4 weeks of growth. The δ¹⁸O values showed seasonal signals, covering a period 1–2 years prior to the time of capture. Isotope ratios at the edge of the otoliths, which is the material deposited at the time of fish capture, corresponded to the coldest seasonal water temperatures, in late winter or early spring. These data provide independent evidence for the season of use of the Skoklefald and Skipshelleren sites.     

Rats cannot have been intermediate hosts for Yersinia pestis during medieval plague epidemics in Northern Europe

The commonly accepted understanding of modern human plague epidemics has been that plague is a disease of rodents that is transmitted to humans from black rats, with rat fleas as vectors. Historians have assumed that this transmission model is also valid for the Black Death and later medieval plague epidemics in Europe. Here we examine information on the geographical distribution and population density of the black rat (Rattus rattus) in Norway and other Nordic countries in medieval times. The study is based on older zoological literature and on bone samples from archaeological excavations. Only a few of the archaeological finds from medieval harbour towns in Norway contain rat bones. There are no finds of black rats from the many archaeological excavations in rural areas or from the inland town of Hamar. These results show that it is extremely unlikely that rats accounted for the spread of plague to rural areas in Norway. Archaeological evidence from other Nordic countries indicates that rats were uncommon there too, and were therefore unlikely to be responsible for the dissemination of human plague. We hypothesize that the mode of transmission during the historical plague epidemics was from human to human via an insect ectoparasite vector.     

Evidence for introduced taro (Colocasia esculenta) and lesser yam (Dioscorea esculenta) in Lapita-era (c. 3050–2500 cal. yr BP) deposits from Bourewa,southwest Viti Levu Island,Fiji

Starch residue, pollen and phytolith analysis was carried out on coralline deposits from a c. 3050–2500 cal. yr BP Lapita site at Bourewa, Viti Levu, Fiji. Starch grains, calcium oxalate crystals and xylem cells of introduced Colocasia esculenta and Dioscorea esculenta were identified, involving a process of elimination of possible taxa by cross-correlation of microfossil types. The data provide an eastward extension of direct evidence of Lapita horticulture in Remote Oceania previously identified in Vanuatu.     

Stable isotope analysis of dog,fox, and human diets at a Late Holocene Chumash village (CA-SRI-2) on Santa Rosa Island,California

Stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope analyses of dog (Canis familiaris), island fox (Urocyon littoralis), and human bone collagen from CA-SRI-2 (AD 130–1830) on Santa Rosa Island, California provide a proxy of diet and the relationships between humans and these animals. Carbon isotopic signatures indicate that Native Americans and their dogs at CA-SRI-2 subsisted almost exclusively on marine resources, while the island fox ate primarily terrestrial foods. Nitrogen isotopes and archaeofaunal remains indicate that humans and dogs also ate higher trophic level foods, including finfishes, marine mammals, and seabirds with smaller amounts of shellfish. The CA-SRI-2 island foxes appear to have eaten higher amounts of terrestrial foods, similar to the diets observed in modern fox populations. These data generally confirm the commensal relationship assumed to exist between domesticated dogs and people, but the carbon isotopic composition of dogs is enriched ∼2‰ compared to humans. We hypothesize that the difference in carbon isotopes between dogs and humans may have resulted from a higher consumption of C3 plants with lower δ¹³C values by humans, or less likely from the ingestion by dogs of significant amounts of bone collagen, which is enriched by ∼4‰ over associated muscle.