A short note on starch and xylem of Colocasia esculenta (taro) in archaeological deposits from Pitcairn Island,southeast Polynesia

We present results of starch analysis of archaeological deposits from Pitcairn Island. High concentrations of starch grains preserved in cell membranes, and xylem tracheary elements, consistent with introduced Colocasia esculenta (taro) were found. Because of limited age control, we are uncertain if the microfossils are prehistoric. Problems associated with identifying taxa with small starch grains in extractions from weathered deposits are highlighted.     

Ancient DNA analysis of desiccated wheat grains excavated from a Bronze Age cemetery in Xinjiang

Wheat has been one of the most important crop in Eurasia since the Neolithic period. Understanding the spread of wheat cultivation is crucial to understanding the spread of agriculture as a whole and the interactions between prehistoric populations across the Eurasian continent. However, the routes by which wheat cultivation spread eastwards have been poorly understood to date, due to the scarcity of plant remains recovered from archaeological sites. Desiccated wheat grains excavated from the Xiaohe cemetery in Xinjiang, and dated to the early Bronze Age, show excellent DNA preservation. Here we present an ancient DNA (aDNA) analysis of wheat (Triticum sp.) grains excavated from Xiaohe and provide the first definitive evidence for bread wheat in China during the Bronze Age. The nuclear ribosomal DNA internal transcribed spacer regions (ITS1 and ITS2) and the intergenic spacer region (IGS) were amplified. The IGS region within the D genome of wheat has a 71 bp insertion that is absent from corresponding regions in the A and B genomes. The results showed that the Xiaohe wheat showed most sequence similarity to hexaploid bread wheat (Triticum aestivum), including the characteristic insertion into the D genome. The presence of bread wheat at the Xiaohe cemetery is discussed in relation to it having spread into Xinjiang by the Bronze Age, providing new insight into the origins of bread wheat in East Asia.     

Neolithic Hedgehogs (Erinaceus europaeus) from the Island of Gotland show early contacts with the Swedish mainland

Previous research probing early migrations and contacts in the Baltic Sea area is characterized by the analysis of different chronologies and subsistent strategies on all sides of the Sea. Several studies performed on artifact typology, ceramics, grave rituals and physical anthropology ended with varying results. Although the question of human origins remains inconclusive, in this study, we rely on the phylogeography of an animal associated with humans to elucidate findings regarding prehistoric human migration and contacts.Hedgehogs, along with other fauna on Gotland, were brought over to the island by humans. We examined hedgehog mitochondrial DNA from the Pitted Ware Culture (Middle Neolithic). The genetic signatures of the animals on the island were investigated to determine the animal’s origin.From the 23 bones originally examined, twelve bones from all five locations studied yielded reliable results and resembled published extant Erinaceus europaeus sequences from Sweden, Norway and Denmark. We postulate that a western heritage for the Neolithic hedgehogs on Gotland indicates early human contact with the Swedish mainland.     

Comparing seeds/fruits and pollen from a Middle Bronze Age pit in Florence (Italy)

The recovery of a Middle Bronze Age pit in Florence offered the opportunity of studying seeds/fruits and pollen coming from the pit fill layers. Both datasets resulted similar in their mixed composition of cultivated, synanthropic, and wild plants. They represent different traces coming from the plants which were stored and voluntarily or involuntarily introduced into the structure. Foodstuff and fodder storage activities are testified in the pit. Particularly, plant gathering is better represented by seed/fruit remains, while fodder procurement is mainly clarified by pollen. In this study, the use of pollen as evidence of economic activity is validated by the hypothesis that the origin of the pollen in the pit is from the stored plant material more than from the pollen rain. Therefore, comparing these datasets proved to be useful to assess the sources of the plant remains found in the pit, to interpret the origin of the fill layers and make some inferences on the structure and its uses, and to attempt some palaeoethnobotanical considerations.     

Multi-element soil prospection aiding geophysical and archaeological survey on an archaeological site in suburban Sagalassos (SW-Turkey)

In order to take full advantage of the archaeological information contained within buried archaeological sites, it is important to apply an integrative approach combining complementary prospection methods. In this study, geochemical prospection data are combined with archaeological and geophysical survey results on an unexcavated site in suburban Sagalassos (SW-Turkey), with the aim of obtaining better insights into the structural shapes and past functionalities of the area. Spatial and multivariate statistical analyses of the chemical data reveal anomalies of K, P and Zn on a location where archaeological and geophysical results suggest the presence of ceramic producing kilns. These elemental enrichments are thought to result from burning wood or dung as fuel for the detected kilns. In addition, local anomalies of Co, Cr, Fe, Mg, Mn and Ni were found to reflect the working and storage of ophiolitic clays, employed as a raw material for ceramic production. Radiocarbon dating of charcoal in a 2.5 m deep drill core in this zone provides ages between AD 120 and 350 at depths of 50 and 60 cm. Al, As, Ba, Ca, Na, Sr, Ti and Pb are considered geogenic elements in this study. The present study supports the theory that geochemical prospection holds potential as a surveying technique, as it was found that chemical data facilitate the interpretation of structures detected by geophysical and archaeological methods, thereby creating an extra dimension to the interpretation of survey data. The results further argue in favour of using strong-acid extractions and the consideration of a large suite of elements when applying chemical soil survey as an archaeological prospection technique, and highlight the importance of considering site lithology. Multivariate statistics proved to be invaluable in distinguishing anthropogenic from lithological soil patterns.     

Ancient DNA of the Pacific rat (Rattus exulans) from Rapa Nui (Easter Island)

We report analysis of ancient mitochondrial DNA sequences from nine archaeological specimens (8 femura and 1 incissor) of Rattus exulans excavated from Anakena Beach Dune on Rapa Nui. Sequence of a 239-base-pair fragment of the hypervariable mitochondrial control region reveals a single mitochondrial DNA (mtDNA) sequence of all samples corresponding to the R9 haplotype prevalent in East Polynesia. This suggests a single or very limited introduction of Rattus exulans to the island. Rapa Nui, like other remote islands of Polynesia, remained effectively isolated following colonization.     

Ancient DNA analysis on Clonorchis sinensis eggs remained in samples from medieval Korean mummy

In the present study, Clonorchis sinensis (C. sinensis) ancient DNA (aDNA) was successfully extracted from human remains discovered in a tomb dating to the medieval Joseon dynasty of Korea. The presence of C. sinensis eggs was confirmed by microscopic observation, after which a PCR-based aDNA analysis was performed using primer sets designed for the amplification of nuclear ribosomal internal transcribed spacer 1 (ITS1), 2 (ITS2) and mitochondrial cytochrome c oxidase 1 (CO1) genes. The sequences obtained were 100% homologous to some contemporary C. sinensis gene sequences reported from Korea and other East Asian countries. We believe that the results of our analysis expand the temporal and geographical scopes of research on the history of C. sinensis infection in different human populations.     

Sweet potato (Ipomoea batatas) and banana (Musa sp.) microfossils in deposits from the Kona Field System,Island of Hawaii

Analysis of plant microfossils (pollen, phytoliths, starch grains and xylem cells) was carried out on 12 soil samples from a variety of pre-contact archaeological features in South Kona within a portion of what has been termed the Kona Field System, on the Island of Hawaii. The oldest radiocarbon ages of the sampled deposits are 1300–1625 AD and 1310–1470 AD. The pollen and phytolith evidence suggests a change from more to less trees and shrubs in the area as a result of human activity. We found phytoliths of banana (Musa sp.) leaves in most and starch grains and xylem cells of tuberous roots of c.f. sweet potato (Ipomoea batatas) in all of the samples, suggesting that the sampled features were associated with these crops and that both crops were cultivated intensively within the study area. Higher concentrations (volumetric) of starch and xylem in samples from older deposits suggest that cultivation was more intensive then. The apparent absence of starch and xylem remains of other tuberous crops archaeologically identified elsewhere in Polynesia suggests that tuberous cropping within the study area was mono-specific.     

On some remains of dog (Canis familiaris) from the Mesolithic shell-middens of Muge,Portugal

The dog has a unique relationship with humans. This is demonstrated by the number of breeds that exist today and the important role that dogs play in human society. The archaeological record also shows that this relationship began long ago when groups of hunter–gatherers domesticated the wolf probably in several parts of the globe.     

Amplification and sequencing of Trichuris trichiura ancient DNA extracted from archaeological sediments

Despite high prevalence of Trichuris trichiura infection, PCR-based analysis on T. trichiura from archaeological samples has not been established so far. In the present study, we sought to perform PCR-based amplification of T. trichiura aDNA using the sediments from medieval tomb of Korea. The presence of Trichuris eggs were first detected by microscopic observation; then confirmed by PCR-based aDNA analysis. Obtained sequence showed 100% homology to that of the small subunit ribosomal RNA (SSUrRNA) gene of T. trichiura but distinct from that of other Trichuris species. PCR-based aDNA analysis in this study can serve as effective method to confirm the presence of T. trichiura eggs in the soils or coprolites collected from archaeological sites.     

Estimating meagre (Argyrosomus regius) size from otoliths and vertebrae

This study presents a method for predicting meagre (Argyrosomus regius) body size (total length) from otoliths and vertebrae recovered from archaeological sites. The method involves regression equations calculated from a reference collection of 36 meagre skeletons and 113 meagre otoliths (sagitta) and allows the simultaneous estimation of original body size and minimum number of individuals (MNI) from archaeological bone structures. We selected the following measurements to predict meagre body size: greatest dorso-ventral height, greatest mediolateral breadth, and greatest anteroposterior length of the vertebrae centra; maximum anteroposterior length, medial anteroposterior length, and dorso-ventral height of the sagitta. Our results show that the original body size of meagre can be accurately predicted from many bone measurements (r² range: 0.921–0.992). We exemplify the use of the regressions in the assessment of size variation and MNI of meagre from four Portuguese Mesolithic sites. We show that regression results provide additional insight into the significant role that this fish played in the subsistence of coastal fisher-hunter-gatherers, who targeted medium-sized animals but were also capable of acquiring rather larger specimens.     

Disentangling long bones of foxes (Vulpes vulpes and Alopex lagopus) from arctic archaeological sites

To discern the presence of two anatomically close species within an archaeological assemblage is always a problem for the analyst. A particularly interesting case is the distinction between the red fox (Vulpes vulpes) and the arctic fox (Alopex lagopus), which is often determined using non-rigorous criteria and producing results that must be regarded with caution. Classical methods for separating these two species and determining sexual dimorphism, and eventually the sex ratio, such as analyses using uni- and bivariate plots, are based on arbitrarily fixed limits between sexes. In this text, a more robust statistical method termed mixture analysis (MA) is used to determine the species of limb bones from foxes. First, the MA is applied to a sample of each species using metric data taken from a collection of present-day fox skeletons. Afterwards, the MA is applied to archaeological samples dated to the Dorset period and retrieved from the Tayara site (near Salluit, South Hudson Strait, Nunavik). The results clearly demonstrate that the greatest length (GL) of long bones, especially the humerus and tibia, is the best measurement for distinguishing the species, followed by the distal breadth and the proximal breadth; and that GL is better suited for sexing foxes. The results of MA applied to the Tayara site collection lead to the conclusion that the red fox is present along with the arctic fox among the assemblage, and that there is no fox body size change during the last two millennia in the Eastern Arctic.     

Petrographic and geochemical analysis on the provenance of the Middle Bronze and Late Bronze/Early Iron Age ceramics from Didi Gora and Udabno I, Eastern Georgia

We report thin section petrographic and geochemical analysis of a total of 20 Middle Bronze, Late Bronze/Early Iron Age ceramics excavated from Didi Gora and Udabno I located in the Eastern part of the Republic of Georgia and 31 clay samples from eight different regions in the surrounding areas of the sites. The major and trace element compositions of the ceramics and clays were determined using a wavelength dispersive X-ray fluorescence technique. The results indicate that the ceramics were manufactured from local clays in Eastern Georgia, mainly from two local clays without any preference of one of the sources during the Middle Bronze, Late Bronze/Early Iron Age.     

Roman agriculture in the conventus Bracaraugustanus (NW Iberia)

A review of archaeobotanical data, together with new, still unpublished data, from Roman sites in conventus Bracaraugustanus suggests the Romanization of the northwest Iberia brought little changes to the agricultural strategies of local communities in indigenous-type settlements. The main crops remain the same as in the Iron Age: Triticum aestivum/durum, Panicum miliaceum, Hordeum vulgare subsp. vulgare, Triticum turgidum subsp. dicoccum and T. aestivum subsp. spelta, Avena and Vicia faba. The first secure reference for the cultivation of Secale cereale in the region comes from a Roman context, suggesting that it was introduced in this period, although it probably remained a minor crop.     

Geoarchaeological insights from a Roman age incineration feature (ustrinum) at Enconsta de Sant'Ana (Lisbon,Portugal)

Ustrina are incineration funerary structures that are relatively common in Roman age cemeteries. Salvage excavations at Encosta de Sant'Ana (Lisbon, Portugal) in 2002 brought to light a part of the necropolis of the Roman town of Olisipo, including some ustrina. One of them, designated Burial 1 during fieldwork, is analyzed here from a geoarchaeological viewpoint to understand the formation processes of such an archaeological feature, namely its construction technique, use and function. The study of site context and subsurface pedofeatures, and the application of archaeological soil micromorphology, revealed that the ustrinum was constructed digging a hollow in bedrock and building a mud-brick ridge around it, with raw material taken from the local bedrock. The structure was used at various times and remodelled at least once, and its base was not always thoroughly cleaned – according to the custom called “pars pro toto” by Roman authors – and probably left open when not in use.     

Middle Stone Age pièces esquillées from Sibudu Cave,South Africa: an initial micro-residue study

In this paper I present the results of a micro-residue study conducted on ten pièces esquillées (scaled pieces) from Sibudu Cave, South Africa. These artefacts are associated with the Howiesons Poort Industry (∼61.7 and ∼64.7 ka years ago at Sibudu), representing part of the later phase of the Middle Stone Age. Until now, it was unclear on what these pieces were used, and whether they were functional. Previous experimental use-wear work tentatively pointed towards bone processing. However, replication work on stone tool production technology suggests that pièces esquillées are merely the by/end-product of bipolar knapping. I used residue analysis on the Sibudu artefacts because this alternative method has the potential to identify if they were used, and if so, illuminate the specific materials the pieces were used on. Although the sample is small, all the pièces esquillées reveal a clear animal processing signal. There are some bone deposits on the utilised edges that may substantiate bone processing, or perhaps a bone hammer was used with them, but additional study, including Later Stone Age artefacts, is needed to assess the feasibility of these observations. It remains possible that the artefacts are core reduced pieces that were subsequently used as tools or simply knapped with a bone hammer.     

The first archaeometric data on polychrome Iron Age glass from sites located in northern Italy

A large sample set of transparent and opaque glass artefacts recovered from Etruscan contexts in northern Italy (Bologna and Spina (FE) necropoleis) and dated to a period between the 6th and 4th century BC are analysed in this paper. Samples of highly decorated beads, spindle whorls and vessels of the ‘Mediterranean Group I’ (Alabastron, Oinochoes, Amphoriskos) were selected in order to determine whether these different glass artefacts were produced at the same manufacturing site. While the vessels almost certainly originate from Greece, the beads could derive from a more ancient local production ascertained at the site of Frattesina (Rovigo, Italy) and dated to the Bronze Age.     

The Terramare and the surrounding hydraulic structures: a geophysical survey of the Santa Rosa site at Poviglio (Bronze Age,northern Italy)

The Terramara Santa Rosa is a Middle and Late Bronze Age archaeological site located in the Po alluvial plain, northern Italy. It is constituted of two moated villages delimited by earthen ramparts. The peripheral structures of the site are sealed by fine-textured flood plain deposits and they have not been fully explored through excavation due to their large extent. Because the shape of the villages and their relation to moats and the fluvial network are of paramount importance to understanding the landscape management and the use of water resources in the Terramare civilisation, a geophysical survey was planned to extend the results of the existing archaeological excavations to the site scale. A frequency-domain electro-magnetic sounding (FDEM) and electrical resistivity imaging (ERI) covered an area of approximately 26 ha; ERI was conducted for a total length >9000 m. Despite the predominance of electrically conductive fine-textured sediments, electrical resistivity anomalies were observed and they were attributed to subtle lithological differences in the sedimentary context of the alluvial plain. The geophysical interpretation, after the calibration with the excavation data, revealed the structures of the Terramara and of the surrounding hydraulic network, which are not visible at the surface due to flood plain deposits. The Santa Rosa site was founded in a favourable geomorphological position, on the top of a crevasse splay lobe of the adjoining Po palaeo-channel, rising above the surrounding alluvial plain. The Terramara and their surroundings were delineated through an artificial modification of this pre-existing crevasse splay lobe and a well-targeted urban design, with the objective of diverting water, most likely from a palaeo-channel of the Po River, through the digging of peripheral moats used to collect water around the site and to distribute it to the surrounding fields for irrigation. The water management documented by this study in the Terramara Santa Rosa can be considered as paradigmatic for the whole Terramare civilisation, which is therefore responsible of the introduction of the irrigated agriculture into western Europe for the first time.     

Palaeochannels of the Balkh river (northern Afghanistan) and human occupation since the Bronze Age period

In 2002, the unexpected discovery of Hellenistic architectural blocks, buried in 8 m of alluvium, found displaced in a former bed of the river of Balkh, brought about a programme of surveys and excavations under the aegis of the French Archaeological Mission in Bactria. The geoarchaeological side of the programme focused on the drawing of a regional geomorphological map then on the identification, mapping and dating of the palaeochannels of the river of Balkh. We established the chronology of several fluvial channel generations by association with archaeological settlements of different eras (Bronze Age, Bactro-Achaemenid period, Kushan period, Islamic period) and with optically stimulated luminescence dating carried out on alluvium deposits. The present riverbed leading up to Aqcha was formed during the Islamic period. We suspect the primary agent of this change in the river channel to, be tectonic activity and the activation of a blind fault crossing the floodplain. We identified a northern Bactro-Achaemenid aqueduct, currently located in the desert area separating the plain of Balkh from the Amu Darya.     

Reappraisal of ‘chronospecies’ and the use of Arvicola (Rodentia,Mammalia) for biochronology

The water vole, genus Arvicola, is characterised by a broad geographic distribution throughout Europe and is widespread during the late Middle and Upper Pleistocene. This genus is used as a major biostratigraphic tool within the Quaternary. Specific determinations using the Schmelzband-Differenzierung-Quotient or SDQ have identified many chronospecies within the fossil species Arvicola cantiana (Hinton, 1910). As SDQ calculation remains limited, this study reappraises the Arvicola genus in terms of morphodiversity and morphospace using outline analysis which takes into account the tooth as a whole. Outline analysis suggests that one single species of Arvicola, A. cantiana, was present during the Pleistocene. This species shows great variability with no trends or patterns in morphospace. Thus, these results call into question the reliability of SDQ for specific determinations and throw doubt on the biochronological framework based on Arvicola.