Patterns of Resource Use and Isotopic Niche Overlap Among Guanaco (Lama guanicoe), Pampas Deer (Ozotoceros bezoarticus) and Marsh Deer (Blastocerus dichotomus) in the Pampas. Ecological,Paleoenvironmental and Archaeological Implications

ABSTRACT

In this paper, we analyze the isotopic niche and resource use of the guanaco (Lama guanicoe), pampas deer (Ozotoceros bezoarticus) and marsh deer (Blastocerus dichotomus) on the temperate plain of the northern Pampa region in the southeast of South America. The measured stable isotope compositions in bone tissue are δ¹³C, δ¹⁵N and δ¹⁸O. All the analyzed samples were recovered from different archaeological sites from the late Holocene. According to the results, the guanaco was strictly confined to the Pampa plain environment, developing a broad niche based on C₃ plants and a variable contribution of C₄ grasses within a grazing trend. The pampas deer preferentially used the Pampa plain, but also the prairies on the borders of the wetland, showing eurioic characteristics and a narrower niche based on C₃ plants. In turn, the marsh deer was strictly confined to wetland environments, developing a C₃ diet–base, within a narrow isotopic niche and stenoic characteristics. The three mammals showed a wide range of intraspecific variability, which was a key factor in their adaptability to spatial and temporal changes in the vegetation coverage. In fact, the temporal trends of their isotopic values were concurrent with the major climatic variations of the Holocene. Differences in the correlations between the values of both carbon sources in both deer species compared with the guanaco suggest a distinct chemical composition of their diet and/or differences in the allocation of nutrients. The isotopic values of nitrogen and spacing of the carbon sources in guanaco (pseudoruminant) and both deer species (ruminants) show no significant differences between them, thus establishing the values for local large herbivores. Significant correlations between δ¹³C and δ¹⁵N were found in the guanaco and marsh deer. The regional and extra–regional variability in the guanaco's δ¹³C_collagen and δ¹⁵N probably reflect the clinal variations in the vegetation coverage and the amount of rainfall. The collagen isotope values in the guanaco throughout the entire Holocene show that the humid Pampa would have shifted between being a more recurrent mesic and temperate plain with minor phases of dry–mesic conditions like during the Little Ice Age, and a humid and warm one at the peaks of the Holocene Thermal Maximum and the Medieval Climatic Anomaly. The guanaco and the pampas deer adapted to all the climatic changes that happened on the humid Pampa until the biological invasions of large European mammals changed the herbivores’ guild composition of this vast plain, pushing them into peripheral habitats due to competition. The within–species variability in isotopic signals through time and space make it necessary to carry out adequate sampling before reconstructing the diets of local past populations.     

Neolithic subsistence patterns in northern Borneo reconstructed with stable carbon isotopes of enamel

The Neolithic period in island Southeast Asia is characterized by various population movements, technological innovations, and the introduction/adoption of agricultural foodstuffs. Human subsistence trends during this period, however, are poorly understood. Broad spectrum foraging is generally assumed for prehistoric groups utilizing rain forest food resources but the degree to which cultigens were part of the dietary repertoire remains unclear. This paper explores human subsistence patterns at three penecontemporaneous Neolithic sites in Sarawak (East Malaysia) using stable isotope ratios of carbon and oxygen derived from tooth enamel apatite. The sites (Niah Cave, Lubang Angin, and Gua Sireh) differ in local ecology and cultural circumstance but all are situated in C₃-dominant lowland primary rain forest. Significant differences in δ¹³C values between sites likely reflect the canopy effect and variations in foraging pattern. Lower values at Lubang Angin suggest dependence upon closed forest foraging. Higher values at Neolithic Niah Cave and Gua Sireh suggest more open forest horticulture and subsistence, including some form of systematic food production, collection, and/or habitat modification.     

Oxygen isotopic evidence of residence and migration in a Greek colonial population on the Black Sea

Oxygen isotope analysis was performed on the enamel of the first and third permanent molars of 60 individuals excavated from the Kalfata-Budjaka necropolis associated with the ancient Greek colony of Apollonia Pontica (5th–3rd centuries BC) on the Black Sea coast of Bulgaria. The objective of this study was to examine the geographic origins and residential histories of these individuals and to compare the isotopic data with archaeological evidence derived from the burials in an attempt to distinguish ‘locals’ from ‘non-locals’. The analysis revealed that 55 of the 60 individuals sampled were either locally born or came from areas outside of Apollonia with isotopically similar δ¹⁸O values. Five individuals were identified as ‘non-local’, and their isotope values suggest that they originated from areas with higher δ¹⁸O_w of precipitation, most likely the Aegean region. Unfortunately, the archaeological evidence associated with these five skeletons provides no clues as to their place of origin.     

Stable isotope evidence for palaeodiets in southern Turkmenistan during Historical period and Iron Age

The subsistence patterns of Iron Age and Historical period humans from south-western Turkmenistan have been reconstructed using the carbon and nitrogen isotopic compositions of archaeological faunal and human bones. A qualitative comparison of the isotopic signatures points to a small proportion of ruminant meat and dairy in human diet for both periods. The ranges of proportions of dietary items yielded by a quantitative approach based on concentration dependent mixing models confirm the high proportions of plant food in the average diet, and show little change in the reconstructed diet for both periods. A comparison of results from zooarchaeological and isotopic approaches illustrates their complementarity in subsistence patterns reconstruction.     

Isotopic Determination of Seasonality in Diet and Death from Nubian Mummy Hair

Sections of human hair from naturally desiccated Sudanese Nubian mummies representing X-Group (AD 350-550) and Christian (AD 550-1300) periods in the Wadi Halfa area have been analysed for their isotope ratios. Because the carbon in hair represents food intake over the growth period of this tissue, analysis comparing segments taken next to the scalp with those further along the shaft can indicate diet just prior to death as well as any recent shift in food consumption. Modern populations in this area practise seasonal crop scheduling which involves the cultivation of C₃ plants (wheat, barley and most fruits and vegetables) in the winter and the hardier C₄ plants (sorghum and millet) in the summer. Shifting isotopic values along the length of hair strands of the ancient inhabitants of this area demonstrate that this practice has its roots at least as far back as the Initial Phase of Intensive Agriculture. The most common season of death appears to have been summer, the time of greatest climatic, nutritional and physiological stress for both ancient and modern inhabitants.     

Interventions in the political geography of ‘libertarian paternalism’

Contributions from political, health and economic geography, social policy and environmental policy studies are brought together here in order to examine the implications of a ‘libertarian paternalist’ political ‘project’ which seeks to react in pragmatic and ideological terms to the excesses and limitations of neoliberalism in liberal democratic contexts. The authors explore the contemporary relevance and historical antecedents of so-called ‘nudge’ tactics of governing, and draw attention to the political geographies encountered in this popular political programme in specific cases.     

Human subsistence strategy at Liuzhuang site,Henan, China during the proto-Shang culture (∼2000–1600 BC) by stable isotopic analysis

Since the discovery of the proto-Shang culture, created mostly by ancestors of the Shang clan from the late Neolithic Age to the early Shang period (∼2000–1600 BC), the subsistence strategy and lifestyle of humans in China during their movement southwards have been a great focus. Chinese literature and archaeological findings suggest that the proto-Shang societies were composed of different cultural groups and had various subsistence strategies. For example, at the Liuzhuang site, three types of burials, i.e., stone coffin, wooden coffin and earthen shaft-pit, are found. The wooden coffin and earthen shaft-pit burials had been adopted locally in the Central Plains since the Neolithic Age while the stone coffin burials were usually used by people living in Northeast China and had never been found in the Central Plains before. In this study, stable carbon and nitrogen isotopic analyses were performed on human bones from the Liuzhuang site and animal bones from Zhangdeng site in Henan province, China to determine whether different social groups had various accesses to food resources and whether their dietary difference was related to inequality in social status. Humans have mean δ¹³C and δ¹⁵N values of −7.6 ± 0.6‰ and 9.6 ± 1.0‰ (n = 19) respectively, which strongly indicates that humans rely primarily on C₄-based food. The main contribution of C₄-based food in their diet is from millet agriculture or animals that consume millet by-products. The isotopic spacing of carbon and nitrogen isotopic values between pigs and humans, between dogs and humans, and between cattle and humans, all imply that these animals were the main meat resources for humans. Surprisingly, the δ¹³C values and δ¹⁵N values of humans with different types of burials are quite close, indicating that they had equal access to food resources. This result suggests that the proto-Shang humans had adapted to the local subsistence strategy, and the local cultural factors in the Central Plains were very well integrated into proto-Shang culture.     

Endangered species,archaeology, and stable isotopes: huemul (Hippocamelus bisulcus) isotopic ecology in central-western Patagonia (South America)

The management and conservation of animal species should be based upon a long-term analysis that considers its geographical distribution and feeding patterns. The study of carbon and nitrogen stable isotopes on skeletal remains provides a quantitative approach to the paleodietary reconstructions and constitutes a potent tool for comparing behavioral aspects of the fauna. In this paper we present the first set of isotopic values for modern and archaeological samples of Huemul (Hippocamelus bisulcus) from the forests of Central-western Patagonia, Chile. Contrary to initial expectations, our analysis indicates that there is no evidence of an important incidence of the canopy effect on the δ¹³Ccollagen values, which we suggest is due to an ecological selection that drives the huemul to focus its predation on open sectors within the forest. On the other hand, a wide range of δ¹⁵Ncollagen values for huemul was verified. This could reflect the N impoverishment of the forest soils and provide an interesting ecological indicator. The long-term information offered by the archaeological record provides the necessary context for decision-making conducive towards the preservation of Hippocamelus bisulcus in Patagonia.     

Modern humans in Sarawak,Malaysian Borneo,during Oxygen Isotope Stage 3: palaeoenvironmental evidence from the Great Cave of Niah

During recent reinvestigations in the Great Cave of Niah in Borneo, the ‘Hell Trench’ sedimentary sequence seen by earlier excavators was re-exposed. Early excavations here yielded the earliest anatomically-modern human remains in island Southeast Asia. Calibrated radiocarbon dates, pollen, algal microfossils, palynofacies, granulometry and geochemistry of the ‘Hell Trench’ sequence provide information about environmental and vegetational changes, elements of geomorphic history and information about human activity. The ‘Hell’ sediments were laid down episodically in an ephemeral stream or pool. The pollen suggests cyclically changing vegetation with forest habitats alternating with more open environments; indicating that phases with both temperatures and precipitation reduced compared with the present. These events can be correlated with global climate change sequences to produce a provisional dating framework. During some forest phases, high counts of Justicia, a plant which today colonises recently burnt forest areas, point to fire in the landscape. This may be evidence for biomass burning by humans, presumably to maintain forest-edge habitats. There is evidence from palynofacies for fire on the cave floor in the ‘Hell’ area. Since the area sampled is beyond the limit of plant growth, this is evidence for human activity. The first such evidence is during an episode with significant grassland indicators, suggesting that people may have reached the site during a climatic phase characterised by relatively open habitats ∼50 ka. Thereafter, people were able to maintain a relatively consistent presence at Niah. The human use of the ‘Hell’ area seems to have intensified through time, probably because changes in the local hydrological regime made the area dryer and more suitable for human use.     

Guanaco (Lama guanicoe) isotopic ecology in southern South America: spatial and temporal tendencies, and archaeological implications

We synthesize and discuss available isotopic data on Holocene guanaco samples from southern South America, extending from Tierra del Fuego Island to northern Patagonia and the Pampean region. We evaluate temporal and spatial tendencies on the basis of 91 samples (mainly based on δ¹³C_collagen values), presenting implications for paleodietary research in archaeology. We conclude that there are no strong correlations with latitude in the macro-spatial scale, while there is a set of interesting patterns at smaller regional scales. These patterns lead us to evaluate the role of ecologic and topographic variables (e.g. canopy effect, variations in altitude, ecotones) in structuring isotopic variability. Finally, on the basis of the present analysis we suggest a number of hypotheses and perspectives for the use of stable isotopes as geographic tracers of guanaco distribution in the past, and specific implications for the paleodietary study of human samples.     

Diversity in foddering strategy and herd management in late Bronze Age Britain: An isotopic investigation of pigs and other fauna from two midden sites

Abstract

Middens of the southern British late Bronze and Iron Age are vast accumulations of cultural debris that can be explained as refuse dumps linked with large periodic feasting events. A distinctive feature of these sites is that their faunal assemblages invariably comprise a considerably higher proportion of pig remains than contemporaneous settlement sites. This paper presents results from a programme of stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope analysis of fauna from two major midden sites, Llanmaes in South Wales and Potterne in Wiltshire. The research aim is to reconstruct husbandry strategies and foddering regimes, particularly concerning pigs, to better understand how the challenges of raising large herds were met. Analysis produced exceptionally wide-ranging results for pigs and other domesticates at both sites, particularly in terms of δ¹⁵N values, demonstrating that diverse foddering strategies were employed. Diversity in the late Bronze Age pig foddering regimes indicates that the Neolithic husbandry practices (focusing on woodland fodder) had not been abandoned, but that new husbandry methods (consumption of household waste) were also being practised, which subsequently became more widely established in the Iron Age. The heterogeneity of signatures suggests that animals may have been husbanded in a piecemeal fashion at a local, household level. This in turn hints that fauna may have been brought to these sites from households across the surrounding landscape, rather than being husbanded by specialist producers in the vicinity of the middens.     

Application of lipid biomarker analysis to evaluate the function of ‘slab-lined pits’ in Arctic Norway

Gas chromatography–mass spectrometry (GC–MS) and bulk carbon isotope determinations have been performed on samples (‘cemented organic residues’, charcoal, sediment and fire-cracked rock) excavated from 12 slab-lined pits from various locations in Arctic Norway to test the premise that these archaeological features were used for the extraction of oil from the blubber of marine mammals, such as seal, whale and walrus. A wide range of lipid compound classes were detected especially in the cemented organic residues and in the charcoal samples. The presence of long-chain unsaturated and isoprenoid fatty acids together with oxidation and thermal alteration products of unsaturated acids such as dicarboxylic acids, dihydroxyfatty acids and ω-(o-alkylphenyl)alkanoic acids suggests that these features were used for marine oil extraction at elevated temperatures. Notably the location of the hydroxyl groups in the dihydroxyfatty acids provides a record of the positional isomer of the precursor fatty acid and allows confirmation that 11-docosenoic (cetoleic) acid, the most abundant C_22:1 isomer in marine oil, was a major component of the original lipid. Further information was provided by the presence of long-chain fatty acyl moieties in surviving triacylglycerols and the presence of cholesterol. A fungal metabolite, mycose (trehalose), was found in all samples apart from a fire-cracked rock and points to microbiological activity in the pits. Bulk isotope analysis conducted on the ‘cemented organic residues’ is consistent with modern reference samples of blubber and oil from seal and whale. These data provide clear analytical evidence of the function of slab-lined pits in the archaeological record and suggest widespread exploitation of marine mammals for producing oil for heating, lighting and myriad other uses in the past.     

Isotopic analysis of archaeobotanicals to reconstruct past climates: Effects of activities associated with food preparation on carbon,hydrogen and oxygen isotope ratios of plant cellulose

The cellulose fraction of botanicals recovered from archaeologically defined strata may be suitable for stable isotope-based palaeoclimatology in much the same manner as cellulose from tree rings has been utilized. However, manipulation by humans is a distinct possibility with such specimens and constitutes a potential source of isotopic alteration early in the post-mortem history of the samples. We studied the susceptibility of plant cellulose to isotopic alteration caused by manipulations related to food processing to determine if discarded plant foods recovered from archaeological contexts are suitable substrates for isotopic palaeoclimatic studies. The carbon and hydrogen isotope ratios of cellulose nitrate and the oxygen isotope ratios of cellulose were determined for Zea mays cobs, Helianthus annus seeds, Agave americana leaves, Pachyrrhizus erosus tubers and a cellulose standard before and after a variety of treatments, including boiling, roasting, fermentation, liming, molding and carbonization. The oxygen and hydrogen isotope ratios (which are climatically sensitive in all plants) and the carbon isotope ratios (which reflect climate only in certain plants) of all samples, except for Agave, were conserved during various food-processing steps. Thus, it appears that the isotope ratios of cellulose and cellulose nitrate prepared from many archaeological food plants, whether processed or uncooked, will reflect in vivo values and can be used to reconstruct aspects of palaeoclimate. Such isotopic palaeoclimatic studies will extend the utility and value of existing collections of ecofacts by opening a unique window for the study of the relationships among climate, plants and man.     

Correlating archaeological and palaeoenvironmental records using a Bayesian approach: a case study from Sutton Common,South Yorkshire,England

This paper presents a case study aimed at correlating archaeological ‘events’ (obtained from radiocarbon measurements and dendrochronology) from the site of Sutton Common with a radiocarbon-dated pollen sequence obtained from a palaeochannel deposit adjacent to the area of the main archaeological activity. It demonstrates the use of a Bayesian approach to quantifying whether the timing of palynological ‘events’ interpreted as reflecting anthropogenic impacts are likely to be associated with archaeological ‘events’. The results suggest that Bronze Age activity in the form of a mortuary enclosure and associated cremation burials are probably not contemporary with the palynological evidence for disturbance to the oak–hazel woodland in this period. Subsequent evidence for local woodland clearance and agriculture is estimated to precede the construction of the large Iron Age enclosure in 372 BC, with increases in ‘anthropogenic indicators’ following this ‘event’. The construction of the site does not appear to have had a pronounced impact on the local vegetation, with hazel the only woody taxon to show clear reductions. Despite the use of a substantial number of oak timbers in the enclosure palisade, percentages of oak remain remarkably stable. Later farming activity on the site probably post-dates the end of activity in the enclosures. The value of the methodology is discussed in relation to quantifiable and robust correlations of archaeological and palaeoenvironmental narratives of landscape and human activity.     

An investigation of diet in early Anglo-Saxon England using carbon and nitrogen stable isotope analysis of human bone collagen

The principal aim of this work is to investigate whether protein sources in human diets in early Anglo-Saxon (5th-7th century AD) England varied with geographic location, or with respect to age or sex. The methodology used was analysis of δ¹⁵N and δ¹³C in human bone collagen from 76 adult skeletons from a total of 18 different cemeteries located in inland, riverine and coastal environments. In order to control for the possible effects of socio-economic status on diet, only high status individuals, as identified by grave goods, were studied. Data were analysed using statistical manipulation of the raw results and using IsoSource (Phillips and Gregg, 2003), a mathematical treatment which models the composition of the protein part of human diets using bone stable isotope ratios when the isotopic values for potential protein sources are known or can reasonably be inferred. The results suggest that protein sources varied little according to geographic location and that terrestrial foods dominated at all locations. However there were some subtle patterns. Slightly elevated δ¹³C in skeletons from coastal locations, and δ¹⁵N in those from riverine sites may indicate that communities in these locations made greater use of marine and freshwater resources respectively. If this is correct then it demonstrates that 5th-7th century communities made some use of wild resources even within a predominantly agrarian economy. In general the analysis of the raw data and the IsoSource results were mutually supportive, and this work demonstrates the value of this twin-pronged approach to analysis of human bone stable isotope data.     

Sr/Sr variability in Puerto Rico: geological complexity and the study of paleomobility

The temptation to use biogeochemical techniques to resolve issues of paleomigration is evident and well intentioned. Knowledge of radiogenic strontium isotope baselines in a region of interest is a sine qua non of such archaeological studies of paleomobility. Here, we present the first detailed study of baseline ⁸⁷Sr/⁸⁶Sr values for the island of Puerto Rico. The high degree of ⁸⁷Sr/⁸⁶Sr variability present in this corpus of modern Puerto Rican bedrock and terrestrial malacological samples (0.70406–0.70909) is a testament to the complex geology of that island. This diversity of ⁸⁷Sr/⁸⁶Sr values makes parsing issues of origin a difficult and highly contingent task. Given these complexities, regional studies seeking to assess paleomigration by such isotopic means should proceed with a great deal of caution.     

Evidence for glass ‘recycling’ using Pb and Sr isotopic ratios and Sr-mixing lines: the case of early Byzantine Sagalassos

The secondary production (working) of glass from imperial to early Byzantine times has been proven at the ancient city of Sagalassos (SW Turkey) by the existence of glass chunks, fuel ash slag and kiln fragments related to glass processing. It had been previously suggested that local green glass might have been recycled from two other locally found glass types (blue glass vessels and chunks and HIMT glass chunks). This paper provides analytical evidence for the recycling of glass next to the use of imported raw glass. The heterogeneous lead isotopic composition of the green and HIMT vessel glass at Sagalassos, with as end members on the one hand the isotopic composition of local blue glass vessels and chunks and on the other hand that of the HIMT glass chunks, could indicate the production of ‘recycled’ glass, although heterogeneous raw materials could have been used. However, the use of Sr-mixing lines confirms local recycling. It is clear that the Sr in the green and HIMT vessels is a mixture of the Sr in the aforementioned end members. It cannot be proved whether the green ‘recycled’ glass was produced from a mixture of chunks alone, or from a mixture of cullet and chunks. Suggestions are made towards the possible origin of the raw materials for the blue and HIMT glass on the basis of Sr isotopic signatures and absolute Sr contents in the glass.     

The ‘walking’ megalithic statues (moai) of Easter Island

Explaining how the monumental statues (moai) of Easter Island were transported has remained open to debate and speculation, including their resource expenditures and role in deforestation. Archaeological evidence including analysis of moai variability, particularly those abandoned along ancient roads, indicates transport was achieved in a vertical position. To test this proposition we constructed a precise three-dimensional 4.35 metric ton replica of an actual statue and demonstrate how positioning the center of mass allowed it to fall forward and rock from side to side causing it to ‘walk.’ Our experiments reveal how the statue form was engineered for efficient transport by a small number of individuals.     

Isotopic analysis of the ecology of herbivores and carnivores from the Middle Pleistocene deposits of the Sierra De Atapuerca,northern Spain

Carbon and oxygen isotope values reveal resource partitioning among the large mammal fauna from three contemporaneous Middle Pleistocene hominid-bearing localities within the Sierra de Atapuerca (northern Spain). Carbon isotope values sampled from the tooth enamel of fauna present during Atapuerca Faunal Unit 6 show that a C₃-dominated ecosystem surrounded the area where fossils were preserved during this time. For the herbivores, Fallow deer isotope values are significantly different from Red deer and horses and show that this species did not forage in open environments at this locality. Red deer and horses show similar feeding strategies with less negative carbon values implying use of more open environments for these taxa. For the carnivores, carbon isotope values for Ursus deningeri are significantly different from either lions (Panthera leo) or foxes (Vulpes vulpes) and support the contention that this species is herbivorous. Special metabolic mechanisms involved in hibernation in U. deningeri might also have influenced its isotope values. The carbon isotope values of remaining carnivores were similar and suggest that each was typically a generalist carnivore, eating a wide variety of prey items. While the isotopic results generally correspond to ecology indicated by previous techniques, this study shows that isotope analyses can provide further insights that alternate techniques do not provide. Isotope analyses can help elucidate the ancient ecology of taxa present in the Sierra de Atapuerca during the Middle Pleistocene allowing for an accurate portrayal of the setting in which humans lived.     

Latitudinal variation in a photoperiod response gene in European barley: insight into the dynamics of agricultural spread from ‘historic’ specimens

Between ca. 6000 BC and ca. 500 BC, barley cultivation spread across the continent of Europe from the extreme south to the extreme north. Carbon-dating would suggest that this spread, and indeed the spread of crop cultivation generally, varied in its pace, with ‘delays’ at certain points along its route. Such delays in the spread of agriculture have been explained as resulting from the slow assimilation of agricultural practices by existing indigenous human populations or as the time taken for the crops to adapt to novel climatic conditions, such as altered temperature regimes and day-lengths. A mutant form of the photoperiod response gene, Ppd-H1, causes barley to be non-responsive to long days, while the wild-type responsive form allows plants to flower in response to long days. We sequenced this gene in 65 ‘historic’ barley accessions, from the late 19th and early 20th centuries, in order to explore the potential role of environmental adaptation in the spread of agriculture. We chose to use ‘historic’ material, to complement the richer patterns in extant genetic lines, by spreading the data range in both time and space. Our ‘historic’ barley data shows a latitudinal divide in the Ppd-H1 gene similar to that found in extant lines, but with clearer geographical resolution, and extending northwards into the Arctic Circle. We discuss the implications of our results in relation to the dynamics of agricultural spread across Europe.