Bone preservation,decay and its related conditions in ancient human bones from Kuwait

By means of scanning electron microscopy, four out of a series of twelve inhumations from the Hellenistic Period were examined for traces of the decay process. The changes represented the result of erosion and biological decomposition of human bones on a small island in the northern part of the Persian Gulf over a period of ca. 2200 years. Special emphasis was given to bone preservation and blood cell survival, and to bone changes due to physicochemical erosion and fungus, bacterium, insect and plant-root activity. Related soil and climatic conditions were taken into consideration. The observations should be evaluated to understand the unusual state of preservation of the bones and to avoid possible misinterpretation of pseudo-pathological bone changes as ante-mortem pathology.     

Bone preservation in human remains from the Terme del Sarno at Pompeii using light microscopy and scanning electron microscopy

Archaeological bone can show marked and complex alterations depending on the environment in which it was buried. In this study, the state of preservation of 27 femurs recovered from the archaeological site of Pompeii was evaluated by light microscopy and scanning electron microscopy. Most of the bone samples, prepared by the grinding method, showed good histological preservation, although they were characterized by microfissures (microcracking). Nine bone samples showed different states of histological preservation, including worst preservation (two femurs), due to diagenetic processes. Cryostat bone sections stained with thionin or 4′,6′-diamidino-phenylindole (DAPI) revealed the persistence of DNA within some osteocyte lacunae. Scanning electron microscopy analysis showed that ultrastructural characters, such as lamellae and collagen fibres, are recognizable only in unaltered bone. Our results reveal that most Pompeian samples are well preserved since they have a bone microstructure virtually indistinguishable from that of fresh bone. In methodological terms, although each of the various morphological methods used contributes information, histological and histochemical analyses are the most informative for studying the preservation state of bone and allow for rapid essential screening of archaeological bone.     

A technological study of ancient faience from Egypt

The chemical compositions and microstructures of some 35 faience objects from Egypt spanning the period from the Middle Kingdom through to the 22nd dynasty are determined using analytical scanning electron microscopy. Replicate faience beads glazed in the laboratory using the efflorescence and cementation methods are similarly investigated. In efflorescence glazing, there appears to be preferential efflorescence of soda over potash, and in cementation glazing, preferential take up of potash over soda into the glaze. These data are then used to try to infer the raw materials and methods of glazing employed in the production of the ancient faience. The glaze/glass phases present in the faience differ significantly in composition from that of New Kingdom glass. This could be due either to the use of different plant ashes or to changes in the composition of the plant ashes during the production of faience and/or glass. Although it is only rarely possible to determine with certainty whether ancient faience was glazed by efflorescence, cementation or application, the observed microstructures provide an indication of the approach adopted to achieve desired performance characteristics such as strength.     

Eggshell and the archaeological record: new insights into turkey husbandry in the American Southwest

Eggshell in the archaeological record has interpretive potential beyond simple species identification. Structural features of eggshell itself indicate the degree of development of the embryo. Using a scanning electron microscope, a reference collection of modern eggshell characteristics has been produced that can be used for species determination and for the assessment of embryo development of prehistoric eggshell. We apply this technique to a sample of prehistoric eggshell from northwestern New Mexico and conclude that purposeful breeding of captive turkey populations is present during the 12th century AD. These preliminary results suggest a behavioral shift in turkey husbandry, concomitant with the well documented phenomenon of intensified turkey utilization after about AD 1100 in the American Southwest.     

The production technology of Egyptian blue and green frits from second millennium BC Egypt and Mesopotamia

The microstructures and chemical compositions of some 55 Egyptian blue and green frit samples from New Kingdom Egypt and 15th century BC Mesopotamia are determined using analytical scanning electron microscopy, the main focus being on frit cakes, powder residues, and frit vessel and bead fragments from one of the “factory areas” at Amarna in Middle Egypt. Replicate Egyptian blue frits produced in the laboratory are similarly investigated. Comparison of the microstructures of the ancient and replicate samples suggest that frit cakes are the primary product, and that these were ground to produce the powder, and then moulded to shape and refired to produce the vessels and beads. Egyptian blue and green frits are shown to be distinct pigments, the pigment produced depending on the relative proportions of copper oxide and lime in the mixtures. The bulk and glass phase compositions are used to try to infer the different sources of the quartz, lime, copper and alkali flux used in the production of frits from Egypt and Mesopotamia. An estimate is made of the scale of production of Egyptian blue frit in New Kingdom Egypt.     

Investigation of chemical changes in bone material from South African fossil hominid deposits

The bone fragments of the Australopithecus Africanus from the dolomitic cave in the Cradle of Humankind in South Africa have been studied by the use of several spectral techniques. The aim was to establish their degree of preservation and possibilities of inferring the life conditions from them. X-ray diffraction studies revealed the transformation of the mineral components partially into fluoroapatite form with addition of goethite, birnessite and quartz phases and with surprisingly well preserved collagen remains. Several important chemical elements were detected by using the electron (EPMA) and synchrotron-based X-ray fluorescence (XRF) microprobes. Among them, Sr and Zn were distributed in a way following the distribution of Ca, the main element of the bones. We suspect the immanent crystallographic substitution of Ca in this case in native bone. Iron followed the distribution of Sr but while Sr is distributed in a continuous way in more rigid locations, iron occurs mainly in the edge zones of the bones and in spot-like inclusions inside. Some part of the spots located in a very edge of bone is also filled with Mn and Cu. It suggests that the porosity and fracture of bones plays a more significant role in the localization of Fe, Mn and Cu. They also form a rigid thin layer (100–250 μm) avoiding further penetration. As (as AsO₄³⁻?) distribution pattern is unique and seems to occur in those locations where the concentrations of Sr are small. The conditions for bone penetration as determined from Eh-pH diagrams suggest that is rather impossible to preserve the organic matter in locations of Mn and also that Mn and Fe hardly can meet in the same spots.     

Identification of fossil hairs in Parahyaena brunnea coprolites from Middle Pleistocene deposits at Gladysvale cave,South Africa

This research focuses on scale pattern and cross sectional morphology of hair to identify an expanded sample of fossil hairs from Parahyaena brunnea coprolites from Gladysvale cave in the Sterkfontein Valley, South Africa. The coprolites are part of a brown hyaena latrine preserved in calcified cave sediment dated to the Middle Pleistocene (257–195 ka). Forty-eight fossil hairs were extracted from 12 coprolites using fine tweezers and a binocular microscope, and examined using a scanning electron microscope. Hair identification was based on consultation of standard guides to hair identification and comparison with our own collection of samples of guard hairs from 15 previously undocumented taxa of indigenous southern African mammals. Samples were taken from the back of pelts curated at the Johannesburg Zoo and Ditsong National Museum of Natural History (formerly Transvaal Museum, Pretoria). Based on the fossil hairs identified here, this research has established that brown hyaenas shared the Sterkfontein Valley with hominins, warthog, impala, zebra and kudu. Apart from humans, these animals are associated with savanna grasslands, much like the Highveld environment of today. These findings support the previous tentative identification of fossil human hair in the coprolites, provide a new source of information on the local Middle Pleistocene fossil mammal community, and insight into the environment in which archaic and emerging modern humans in the interior of the African subcontinent lived.     

Ancient bacterial DNA (aDNA) in dental calculus from archaeological human remains

The purpose of this study was to identify reactive bacterial aDNA in archaeological human dental calculus. Dental calculus was collected from a middle/late Neolithic human skull from Hulbjerg passage grave, Langeland, Denmark and prepared for transmission electron microscopy (TEM) or gold-labeled antibody TEM. TEM showed calcified, as well as non-calcified bacteria. Immunogold labeling occurred over the cytoplasmic portions of the sectioned bacteria. The result demonstrated that it is possible to identify aDNA sequences from bacteria in archaeological material of considerable age by this technique.     

Wild at heart: Approaching Pitted Ware identity,economy and cosmology through stable isotopes in skeletal material from the Neolithic site Korsnäs in Eastern Central Sweden

The Middle Neolithic Pitted Ware Culture on the Baltic Sea islands comprised a common identity distinguished, in part, by an almost exclusively marine diet. Based on evidence from the first stable isotope analysis on Pitted Ware skeletal material from the Eastern Central Swedish mainland, we suggest that this identity was shared by PWC groups in the archipelago of the west side of the Baltic. Fifty-six faunal and 26 human bone and dentine samples originating from the Pitted Ware site Korsnäs in Södermanland, Sweden were analysed, and the data clearly shows that the diet of the Korsnäs people was marine, predominantly based on seal. The isotope data further indicate that the pig bones found in large quantities on the site emanate from wild boar rather than domestic pigs. The large representation of pig on several Pitted Ware sites, which cannot be explained in terms of economy, is interpreted as the results of occasional hunting of and ritual feasting on wild boar, indicating that the animal held a prominent position, alongside seal, in the hunting identity and cosmology of the Pitted Ware people. Further, eleven new radiocarbon dates are presented, placing the Korsnäs site, with a large probability, within Middle Neolithic A.