Distinguishing between archaeological sheep and goat bones using a single collagen peptide

We describe a method of isolating and analyzing a single collagen peptide able to distinguish between sheep and goat bone collagen. The 33 amino acid peptide from both sheep and goat collagen was sequenced and shown to differ between the two species at two positions. Analysis of a range of caprines indicated that the sequence changes occurred between the divergence of the Himalayan tahr (Hemitragus jemlahicus) and the ibex (Capra ibex) and that the proposed goat marker is diagnostic of all Capra species and breeds. The survival of these markers in archaeological bones was tested using a set of 26 ovicaprid specimens from Domuztepe, a Neolithic site in south central Turkey. These markers were used to test the osteological determination of 24 of the Domuztepe bones, and determine the species for two immature specimens. The collagen-peptide method has advantages over other non-morphological methods of sheep/goat distinction because of the long-term survival of collagen over other biomolecules such as ancient DNA. The results also highlighted the problems in relying upon one morphological criterion, in this case on the distal radius, to distinguish between sheep and goat bones.     

Sexual dimorphism in limb bones of ibex (Capra ibex L.): mixture analysis applied to modern and fossil data

Estimating sex ratios of fossil bone assemblages is an important step in the determination of demographic profiles, which are essential for understanding the palaeobiology and palaeoethology of any particular species, as well as its exploitation patterns by humans. This is especially true for ibex (Capra ibex), which was a main source of food for hominids during Pleistocene times. Classical methods for determining sexual dimorphism and sex ratio, such as analyses using uni‐ and bivariate plots, are based on an arbitrary fixing of limits between sexes. Here we use a more robust statistical method termed mixture analysis (MA) to determine the sex of postcranial remains (long bones, metapodials and tarsals) from ibex. For the first time, we apply MA to both a modern and a fossil sample of one species, by using metric data taken from (i) a collection of present‐day ibex skeletons and (ii) a Palaeolithic sample of the same species. Our results clearly show that the forelimb (humerus and radius) is more dimorphic than the hindlimb (femur and tibia) and is therefore better suited for sexing ibex. It also appears that metapodials should be used carefully for estimating sex ratios. On the basis of these results, we propose a classification of bone measurements that are more or less reliable for sexing ibex. The results of MA applied to the ibex fossil bones from the Upper Palaeolithic site of the Observatoire (Monaco) lead us to the conclusion that this assemblage consists of a majority of males. The quantitative estimations calculated by the MA make it possible to compare the size of Pleistocene and modern ibex for the whole set of variables used in this study. Copyright © 2006 John Wiley & Sons, Ltd.     

A guide for the distinction of species, sex and body side in bones of sheep and goat

Morphological criteria are described that were found to be the most successful for distinguishing between skeletal fragments of adult sheep (Ovis) and goats (Capra) in the Western European, early medieval sites of Haithabu and Oldenburg in Holstein. It is emphasized that a sufficiently large sample should be observed. In addition, methods are given for distinguishing sex in the pelvis, and body side in distal metacarpus and metatarsus ends.     

Analysis of enamel ultrastructure in archaeology: The identification of Ovis aries and Capra hircus dental remains

Ultrastructural features of deep, intermediate and superficial enamel were quantitatively assessed for the mandibular first permanent molars of 10 specimens each of the domestic sheep (Ovis aries) and goat (Capra hircus). The means of a number of variables differed interspecifically at high levels of statistical significance, especially with regard to the enamel of intermediate depth. A canonical discriminant function analysis employing nine variables at all three enamel depths resulted in the correct taxonomic assignment of 95% of the specimens comprising the present sample. These results, coupled with the simple preparative and analytical methods presented here, may enable archaeozoologists to utilize enamel ultrastructure in order to discriminate between fragmentary dental remains of these caprine species.     

An assessment of morphological criteria for discriminating sheep and goat mandibles on a large prehistoric archaeological assemblage (Kerma,Sudan)

This paper presents an assessment of all known dental and mandibular morphological criteria for differentiating sheep (Ovis aries) and goats (Capra hircus) using for the first time an archaeological sample of complete caprine skeletons (90 sheep and 13 goats) from burials at Kerma (Sudan, 3rd and 2nd millennia BC). The species determinations were assessed using cranial and post-cranial morphological criteria. Consequently, the reliability (percentage of correct determination) and efficiency (complementary percentage of intermediate scores i.e. neither sheep nor goat) of 38 individual dental criteria could be fully assessed using a prehistoric homogeneous domesticate population. We demonstrate that, for this sample, individual criteria for lacteal teeth are more reliable for sheep (sheep: 95 ± 3%) than the adult premolars (85 ± 5%) and molars (sheep: 88 ± 2%), whereas for goats premolar criteria were more reliable (83 ± 12%). For efficiency, lacteal dental criteria are better (goat: 97 ± 5%; sheep: 95 ± 3%) than those for premolar (goat: 85 ± 10%; sheep: 79 ± 5%) and molar (goat: 82 ± 6%; sheep: 83 ± 2). We also demonstrated that most isolated teeth can be determined with less than 10% error. However, on average, within specific age classes (0–1 year, 1–4 years and more than 4 years), isolated teeth increased in reliability and decreased in efficiency. The average reliability of the criteria for complete mandibles for each age class for goats and sheep was 100%, when the efficiency was 67, 40 and 50% for goats and over 90% for sheep. This is due to the effect of age on the efficiency of isolated criteria and the poor performance of specific criteria mainly those P3, M1 and M2. We conclude that separate species kill-off profiles are possible. The effect of age on dental criteria would not significantly change the interpretation for specific subsistence strategies focused on one particularly species.     

A radiographic study of the extent of variation in cortical bone thickness in soay sheep

In order to assess the effect of nutrition on bone mass, radiographs of metopodia from skeletal collections of Soay sheep (Ovis aries) were taken of animals subject to seasonal stress (from the island of Hirta, Hebrides) and those living in good nutritional conditions (from the Institute of Animal Physiology, Babraham, Cambridge). A third set of radiographs was taken at 3 month intervals over a single year on living Soay sheep kept under good conditions at the Rowett Research Institute, Aberdeen.Measurements were taken on the radiographs of length, minimum shaft width (SW) and medullary cavity width (MCW), while combined cortical thickness (CCT) was calculated. The ewes from Hirta had significantly lower values for CCT than those from Babraham, whereas the rams showed no significant reduction in CCT. Since no seasonal variation in CCT was detected in the well nourished Rowett ewes, this indicates a differential effect of poor environmental conditions on bone metabolism in ewes. It is proposed here that this may be related to the additional stress imposed by gestation and lactation on female animals living under conditions of seasonally restricted food supply.     

Morphological distinctions between the mandibular teeth of young sheep, Ovis, and goats, Capra

Morphological distinctions between the mandibular teeth of young sheep (Ovis) and goats (Capra) are described, and their value in the study of bone samples from archaeological sites is briefly discussed.     

Mitochondrial DNA Analysis of Ancient Domestic Goats in the Southern Caucasus: A Preliminary Result from Neolithic Settlements at Göytepe and Hacı Elamxanlı Tepe

This study presents preliminary results of mitochondrial DNA analyses of modern and ancient domestic goats in the southern Caucasus in order to examine their phylogenetic relationship with modern and ancient goats. For this purpose, seven ancient samples were obtained from two early agricultural villages in west Azerbaijan (Göytepe and Hacı Elamxanlı tepe, dated to ca. 6000–5500 cal bc , the Pottery Neolithic period), in addition to five modern goat samples in the same region. In the study, mitochondrial DNA segments of the control region (216 bp for the Neolithic samples and 481 bp for the modern samples) were amplified, and phylogenetic analyses were performed using previously published reference DNA sequences. As a result, all the haplotypes found in this study were grouped in the haplogroup A of goats. The finding of the haplogroup A among domestic goats in the southern Caucasus in the early sixth millennium bc can be interpreted as part of the geographic expansion of this lineage from the areas of initial domestication to surrounding areas that include also South and Southeast Europe. In the southern Caucasus, the haplogroup A probably continued to be a major lineage among domestic goats since their emergence in this area to the present. In contrast, this lineage has not been detected among local wild goats including Capra aegagrus , indicating the external origin of domestic goats. This possibility is consistent with archaeological records that indicate sudden appearance of agricultural lifeways in the southern Caucasus and cultural connections with northern Mesopotamia. Copyright © 2016 John Wiley & Sons, Ltd.     

Distinguishing sheep and goats using dental morphology and stable carbon isotopes in C4 grassland environments

Distinguishing between sheep and goats in C₄ grass environments using new dental morphology criteria and enamel bioapatite stable carbon isotope ratios (δ¹³C) was tested on 35 modern individuals from the Central Rift Valley of Kenya. Two morphological criteria on the second and third lower molars, one of which had been previously partially described by Halstead et al. [Journal of Archaeological Science 29 (2002) 545], were found to be highly reliable in this population. Identification of species using carbon isotope ratios is made possible in some circumstances by differences in the feeding behavior of sheep, which are mainly grazers, and goats, which are mainly browsers. In environments where C₄ grasses predominate, sheep include a higher proportion of C₄ plants in their diet, and thus have higher δ¹³C values than goats. In the present study, the annual range and seasonal variation of carbon isotope ratio of diet of sheep and goats was measured from intra-tooth sequential analysis. Although the ranges of goat and sheep δ¹³C values overlap, those higher than −3.4‰ all belong to sheep; values lower than −5.2‰ all belong to goats. There is no overlap of the mean δ¹³C values by tooth, which range from −11.8‰ to −4.2‰ for goats, and from −3.1‰ to −1.3‰ for sheep. These results suggest that carbon isotope analysis of bone collagen and/or apatite will also distinguish sheep from goats in tropical C₄ grasslands. Application of the δ¹³C criteria to archaeological material must be restricted to C₄-dominated environments, and where potential access to C₃ plants (mobility, foddering) can be assessed. The utility of these morphological and isotopic criteria for differentiating sheep and goat breeds in other regions remains to be evaluated.     

Absolute age and tooth eruption and wear sequences in sheep and goat: determining age-at-death in zooarchaeology using a modern control sample

This paper seeks to improve our understanding of the relationship between mandibular tooth eruption and wear sequences and absolute age in modern sheep and goat. While the relationship between tooth eruption and wear and absolute age has been investigated for primitive breeds in parts of the Old World, our pilot study extends the applicability of this research to modern breeds and to another geographic region (North America). A modern control sample of two species of domestic caprines – Ovis aries (sheep) and Capra hircus (goats) from Manitoba, Canada – are used to evaluate the two major systems for analyzing mandibular tooth wear and eruption (Payne and Grant) and for assigning an absolute age-at-death. In order to compare the two systems, an absolute age for Grant's Mandibular Wear Stages was established for the first time, previously demonstrated only for Payne's system. The analysis confirmed that there is a very high correlation between the known age and estimated age based on tooth eruption and wear. However, Payne's system generated far coarser age-at-death profiles, which lowers its utility in constructing harvest profiles. Grant's system is recommended for ageing be utilized since it can yield more sensitive age estimations. In conclusion, it is possible to utilize the modern caprine breeds to establish the relationship between tooth eruption and wear and absolute age to reconstruct age and season of death of archaeological samples.     

Sorting the Sheep from the Goats: Morphological Distinctions between the Mandibles and Mandibular Teeth of AdultOvis and Capra

Diagnostic criteria are described for the taxonomic discrimination of adult mandibles of sheep (Ovis aries) and goat (Capra hircus). These criteria, based on morphological distinctions in the mandible, permanent premolars (P3–P4) and molars (M1–M3), have been successfully tested on a large and diverse body of modern reference material. In conjunction with published work on deciduous premolars, the new criteria enable complete and fragmentary mandibles of young, adult and elderly individuals to be identified to sheep or goat with acceptable levels of accuracy.     

Upper paleolithic ibex hunting in southwest Europe

Beginning in the Solutrean period (c. 20,000 bp) and extending through the Azilian period (c. 10,000 bp), ibex (Capra pyrenaica) hunting was a significant part of intensive terminal Paleolithic subsistence strategies in Cantabrian Spain and along the French Pyrenees. There are several totally specialized ibex-hunting sites, as well as ibex-dominated layers at sites used for other purposes at other times. Some of these sites (i.e. La Riera, Rascaño, Ekain, Erralla, Les Eglises, Balma Margineda) have been excavated recently, and the faunas have been analyzed by J. Altuna, F. Delpech, and D. Geddes. The results, in combination with data from older excavations, are used to explore the tactics and seasonality of prehistoric ibex hunting and to suggest differences in terms of abandoned anatomical elements between sites located very close to kill spots in the ibex habitat (i.e. on steep, rocky slopes) and those which were located at some distance from the probable hunting locations. This analysis is based on the relative utility of different body parts proposed by L. R. Binford, while taking into account other factors such as biased bone preservation and the “schlepp effect”. It helps to refine our understanding of the logistical ibex procurement station as a fairly distinctive kind of prehistoric site in southwestern Europe.     

Intensive hunting during the Iron Age of Southern Africa

Domestic faunal samples from farming sites from southern Africa dating from the Early (～AD 200–900) and Middle (～AD 900–1300) Iron Ages with large faunal samples are typically dominated by sheep/goats (both number of identified specimens and minimum number of individuals for large samples). However, four exceptions to this general pattern from these time periods are Bosutswe, Nqoma (both in Botswana), KwaGandaganda and Mamba (both in KwaZulu-Natal). At these sites, cattle outnumber sheep/goats, which have previously been measured using a Cattle Index. Intensive hunting is investigated at one of these sites, Bosutswe. Using various lines of evidence, including measuring high- vs. low-ranked prey, economic activities, as well as grease extraction and ageing from the most common taxon, plains zebra (Equus quagga), it is suggested that resource depression of wild game likely occurred. This would fit the expectation, based on human behavioural ecology, that as high-ranked game resource diminished over time, more emphasis was placed on cattle herding. The greater emphasis could have influenced descent patterns of people at Bosutswe. By the Late Iron Age (～AD 1300–1820s), cattle dominate most faunal assemblages in southern Africa with large sample sizes, and ethnographic and historical information confirm the central role these animals played in the social, political and economic lives of these farmers.     

Separating Sheep (Ovis aries L.) and Goats (Capra hircus L.) Using Geometric Morphometric Methods: An Investigation of Astragalus Morphology from Late and Final Bronze Age Central Asian Contexts

Many qualitative and quantitative methods for the separation of sheep and goat bones are based upon Middle Eastern, Mediterranean and European specimens. However, these methods may not be as applicable in other geographical contexts due to regional morphological variation. In order to address this, both traditional and geometric morphometric methods were applied to sheep, (Ovis aries L.) and goat (Capra hircus L.) astragali from archaeological sites from Kazakhstan dating to the Late and Final Bronze Age (1900–900 bce ). This exploratory research confirmed that qualitative features remained useful for distinguishing between species, while traditional morphometric methods were unable to conclusively support the field identification of species. Geometric morphometric methods found significant morphological differences between species and confirmed group membership while exploring specific qualitative features that were effective in distinguishing between sheep and goat in Central Asian contexts. Copyright © 2016 John Wiley & Sons, Ltd.     

IRON AGE, HAIRED, ANIMAL SKINS FROM HALLSTATT, AUSTRIA

Summary: A study was made of 229 skins bearing hair or wool from bags and clothing in the Iron Age salt mines at Hallstatt; 87% were from livestock and of these 8% were from cattle, 22% from goats and 70% from sheep. of the sheepskins most were of hairy-medium type (61%) or coarser, while all the textiles examined earlier were of this type or finer; 5% were of hairy type, which first appeared in the Iron Age; 32% were Neolithic survivals with a wild-type coat (no fleece) and two-thirds of these had a Mouflon colour pattern, while the remainder had a range of colours. the cattle were brown and two thirds of the goats were black. All livestock had the small size expected of the breeds of the period. the other skins included possibly chamois and ibex as well as dog, and small fur-bearers.     

Possible Wild Boar Management during the Ertebølle Period. A Carbon and Nitrogen Isotope Analysis of Mesolithic Wild Boar from Fannerup F,Denmark

This paper presents a stable isotope and radiocarbon study on a total of 85 samples of wild boar (Sus scrofa), humans (Homo sapiens), dogs (Canis familiaris), red deer (Cervus elaphus) and roe deer (Capreolus capreolus) from four Late Mesolithic sites in Jutland, Denmark. Four of the eight Sus scrofa samples from one site, the shell midden of Fannerup F, show markedly enriched carbon and nitrogen isotope values, indicating a dietary intake of a substantial amount of marine food. In contrast to standard interpretations of Late Mesolithic animal economy, we suggest that the enriched values of Sus scrofa may be an indication of management by Ertebølle groups in the area that facilitated access to substantial amounts of marine foods for these wild boars compared to contemporaneous conspecifics. The ¹⁴C dates of the Sus scrofa range from 5290 to 4335 cal BC, suggesting that the management of Sus scrofa developed independently of contact with Neolithic societies. Although the sample size remains small, the interpretation of Late Mesolithic animal management adds to the growing evidence for political and economic complexity in the Ertebølle culture.     

A Metrical Analysis of a Collection of Modern Goats (Capra hircus aegargus and C. h. hircus) from Iran and Iraq: Implications for the Study of Caprine Domestication

A critical comparison of two commonly used markers of initial animal domestication, size reduction and demographic profiling, is presented. A metrical analysis of a large collection of modern wild and domestic goats (Capra hircus aegargus and C. h. hircus) from Iran and Iraq clearly demonstrates that sex is by far the most significant factor affecting size in goats. In all post-cranial bones examined, those of males, even the unfused bones of males over 1 year of age, are absolutely larger than the bones of females. Region also plays a significant role in the size of these animals, but domestic status seems to have little impact. Similarly, a re-analysis of archaeological assemblages from Upland Sites the eastern fertile crescent shows no evidence of population-wide size reduction over a period that encompasses the transition from hunting to herding goats in the region, from the Middle Palaeolithic to the Early Neolithic. The apparent rapid reduction in the size of caprines during the early phases of domestication noted in earlier studies is likely attributable to a shift in the sex ratio of the adult breeding population of managed herds towards female domination, plus the differential destruction and loss of the bones of male animals killed at younger ages, as well as biases introduced by standard methodological practices. Building on the earlier work of Hesse, and using the empirical understanding of the factors influencing size in modern goats gained in this study, a new approach is proposed that combines both size and long-bone fusion data to construct high resolution, sex-specific age profiles of male and female goats that can be used to detect the hunting to herd management transition which marks the initial stages of the domestication process.     

Three statistical methods for sex determination in extant and fossil caprines: assessment of the Rupicapra long bones

Sex determination of fossil remains in archeopaleontological contexts is a necessary step in order to gain insights in archaeological and paleobiological parameters such as prey selection and sexual dimorphism. Species with higher sexual dimorphism are more reliably sexed while the sex determination of less dimorphic animals is not attempted. In this study, we compared the accuracy of three different methods in the sex determination of a modern known-sex sample of chamois (Rupicapra pyrenaica parva) from the Cantabrian range (Northern Iberian Peninsula), which is a small-sized ungulate with low sexual dimorphism when compared to other animals in which sex determination has been attempted (i.e., Ursus spelaeus, Capra pyrenaica). The three methods applied were mixture analysis (MA), cluster analysis (CA) and principal component analysis (PCA). Within CA we applied three different analysis: one agglomerative hierarchical method (AH) and two optimal partitioning methods (k-means and robust k-means); and within PCA we applied normal and robust variants of the analysis.     

Well preserved non‐collagenous extracellular matrix proteins in ancient human bone and teeth

Ancient bones in a good preservation state, ascertained by microscopic techniques, conserve extracellular matrix proteins over thousands of years. With new techniques, intact extracellular matrix proteins from ancient bones and teeth are extracted and separated by one‐dimensional and two‐dimensional electrophoresis. Proteins were identified in Western blots by special antibodies against different human extracellular matrix (ECM) molecules of bone. We have confirmed different types of ECM human bone molecules such as osteonectin, osteopontin, and alkaline phosphatase with specific antibodies in human bone samples from different age groups. Additionally, we selected bone samples from different cultural time periods, such as the Middle Ages, the Bronze Age and the Late Pre‐Pottery Neolithic Phase (PPNB), and teeth from individuals from the Early Middle Ages and from the Late PPNB. The survival of intact extracellular matrix proteins in ancient bones and teeth dating from recent times to the Late PPNB, and reliable techniques to identify these proteins, present a great challenge to further research. A Match Set with PD‐Quest 7.2 shows that only 16% of protein spots in the teeth are also found in the bone of the same individual. In combination with the results of macro‐ and microscopic investigation, biochemical techniques will help us in obtaining a better understanding of bone and teeth in health and disease. Copyright © 2006 John Wiley & Sons, Ltd.     

Selection and Exploitation of Macro‐Vertebrate Resources During the Upper Palaeolithic in Northern Spain. New Evidence from Coímbre Cave (Peñamellera Alta,Asturias)

The exploitation of ungulates in the Cantabrian region during the Upper Palaeolithic is characterized by the appearance of progressively specialized hunting strategies, especially during the Magdalenian. This specialization focused on either Iberian ibex or red deer, depending on environmental or topographic features. Red deer, for instance, was hunted mostly on the plains while ibex and/or chamois was hunted in rocky and mountainous areas. Here we present new zooarchaeological and taphonomic evidence from Coímbre cave (northern Spain), a site located in the rugged region between the Picos de Europa and Sierra del Cuera (Asturias) which has evidence for specialized ibex hunting. We discuss the possible reasons for such a selective hunting pattern. While the predominance of mountain species such as Iberian ibex or chamois in the Magdalenian levels suggests prey selection based on topographic or environmental criteria, the predominance of large bovids in the Gravettian level could imply that other alternatives were available. We also provide evidence of a pattern of rabbit exploitation which is unusual by comparison with other Upper Palaeolithic sites of northern Spain, and which taphonomic evidence suggests was due to human activity.