Dirty teeth and ancient trade: Evidence of cotton fibres in human dental calculus from Late Woodland,Ohio

Analysis of ancient human dental calculus for the presence of inclusions related to diet and dental health has been overlooked in anthropological literature. Small particles of archaeobotanical debris, which would otherwise not be preserved in the archaeological record, can become incorporated into unmineralised plaque on teeth during mastication and oral manipulation. When plaque mineralises into calculus, debris is preserved in situ. Samples of dental calculus (n = 18) were collected from the Danbury site (33OT16) in Ottawa County, Ohio and viewed under a scanning electron microscope for inclusions. Analysis yielded a variety of noticeable inclusions, including mineralised bacteria, calcium‐phosphate crystalline structures and numerous phytoliths. Here we report the first evidence of fibres consistent with cotton (Gossypium spp.) embedded in the dental calculus from the Late Woodland component (900–1100 AD ) of the Danbury site. Prehistoric cotton has not been previously documented in Ohio. The distinct morphology of the Danbury cotton and its presence in the Late Woodland component at Danbury suggests long‐distance interaction at a time in Ohio when movement of exotic goods appeared to have diminished. These microscopic remains provide insight into paleoethnobotanical history of ancient Ohioans and attest to how analysis of dental calculus could be used to supplement other paleodietary and archaeological analyses. Copyright © 2010 John Wiley & Sons, Ltd.     

基于人牙结石的淀粉粒证据探讨裴李岗遗址先民植物性食物来源

人牙结石淀粉粒分析是开展古代居民食物来源研究的有效途径之一。用此方法对河南新郑裴李岗遗址仅存的两具裴李岗文化人骨牙齿上的结石开展了淀粉粒分析,成功提取出38颗淀粉粒,其中可鉴定种属的淀粉粒主要来自于栎属、豆科和块茎类等非农作物,少量有可能来自于粟黍类作物。虽然分析样品较少,提取出的淀粉粒数量不多,但这一结果或许表明采集植物有可能构成了裴李岗遗址居民植物性食物的主体。结合贾湖、莪沟、石固和寨根等裴李岗文化遗址的植物考古学研究,裴李岗文化居民的食物结构具有广谱性特征。本研究为全面认识中原地区裴李岗文化居民食物构成和聚落生业经济,深入探讨中原地区早期农业形成过程等提供了新思路和新证据。     

Ancient bacterial DNA (aDNA) in dental calculus from archaeological human remains

The purpose of this study was to identify reactive bacterial aDNA in archaeological human dental calculus. Dental calculus was collected from a middle/late Neolithic human skull from Hulbjerg passage grave, Langeland, Denmark and prepared for transmission electron microscopy (TEM) or gold-labeled antibody TEM. TEM showed calcified, as well as non-calcified bacteria. Immunogold labeling occurred over the cytoplasmic portions of the sectioned bacteria. The result demonstrated that it is possible to identify aDNA sequences from bacteria in archaeological material of considerable age by this technique.     

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Fifty-eight dental calculus samples from medieval and post-medieval skeletons from Vitoria, Spain, and a single sample from an Alaskan Inuit were tested for stable carbon and nitrogen isotope compositions. There was sufficient carbon and nitrogen concentrations to obtain δ¹³C and δ¹⁵N values, and the samples from Spain produced results that were replicable and comparable to European isotope values based on bone collagen collected from literature sources. The Alaskan Inuit calculus sample yielded a δ¹⁵N value of +17.5‰, well beyond the range of the Spanish samples, but consistent with literature data for modern Greenlandic Inuit consuming a diet rich in marine food. There are several potential sources for carbon and nitrogen in calculus. The results of this study yield stable isotope values consistent with those obtained from other biomaterials used as isotope proxies for paleodietary research, including bone collagen, hair, and fingernails, although further work is necessary to verify the fidelity of calculus as an isotope proxy. Many studies in bioarchaeology are precluded by curatorial concerns regarding the destructive analysis of primary biomaterials. However, calculus is an “add-on”, or secondary biomaterial, that is not an integral part of the dental or skeletal system. Hence, its consumption during analysis is technically not destructive. Therefore, isotope analysis of dental calculus may provide a potential new avenue for paleodietary analysis where the use of other primary biomaterials is precluded.     

Dental calculus and other dental disease in a human skeleton of the Okhotsk Culture unearthed at Hamanaka-2 site,Rebun-Island,Hokkaido, Japan

A human skeleton of the Okhotsk Culture from the Hamanaka-2 site of Rebun Island, northern part of Hokkaido, was found with abnormally large deposits of dental calculus, especially on the right upper 2nd and 3rd molars. This may relate to the early loss of the corresponding right lower molars. Root apex cementum hyperplasia and resorption of alveolar bone due to periodontal disease were also observed; these may have been associated with the calculus. The severe dental calculus and other diseases observed appear to have nothing to do with the subsistence pattern and ethnological background of the Okhotsk Culture, but rather with the individual's poor oral hygiene and digestive dysfunction.     

New insights into the consumption of maize and other food plants in the pre-Columbian Caribbean from starch grains trapped in human dental calculus

In a first region wide study, starch grains from human dental calculus from the pre-Columbian insular Caribbean (dating to ca. 350 B.C.–A.D. 1600) are used to identify important plant foods in the diet and to assess potential dietary differences related to age or sex. Results give important insights into pre-Columbian maize (Zea mays) consumption throughout the region, confirming recent studies that indicate that maize was more commonly consumed in the insular Caribbean than originally thought. No age or sex based differences in maize consumption were found. Furthermore, based on the results of new experiments regarding grinding and pressure damage to starch grains, it is clear that maize in the Caribbean was ground, baked and consumed as bread as was the case in large parts of the mainland. Based on our results we tentatively suggest maize consumption in the Caribbean was at least in some cases associated with feasting and ceremonial activities. The variety in other plant foods identified (mostly tuberous root crops) shows that the pre-Columbian inhabitants of the region consumed a broad spectrum, but locally variable diet in which a variety of root crops functioned as staple crops, including marunguey (Zamia sp.) and sweet potato (Ipomoea batatas). We found no indications for the traditionally assumed heavy reliance on manioc (Manihot esculenta) cultivation in the region.     

Diet,Geography and Drinking Water in Polynesia: Microfossil Research from Archaeological Human Dental Calculus,Rapa Nui (Easter Island)

Microfossil analysis of human dental calculus provides consumption‐specific and archaeologically relevant data for evaluating diet and subsistence in past populations. Calculus was extracted from 114 teeth representing 104 unique individuals from a late 16^th to early 18^th century skeletal series on Rapa Nui (Easter Island) to address questions of human–environment interactions and possible dietary preference. Scanning electron microscopy was used in lieu of optical microscopy for its superior depth of field and resolution of surface detail. The calculus microfossil recovery produced 16,377 total biogenic silica microfossils: 4733 phytoliths and 11,644 diatoms. The majority of phytoliths correspond with the Arecaceae or palm family (n = 4,456) and the minority corresponds to the Poaceae or grass family (n = 277). Because of the relatively large sample size, we were able to test hypotheses related to age cohort, sex, dental element and geographic region. Results indicate no significant difference in phytolith or diatom recovery based on age cohort or sex. The high frequency and proportion of Arecaceae phytoliths found in calculus extracted from the anterior dentition suggests consumption of soft or cooked foods containing palm phytoliths and the high frequency of diatoms recovered from the southern part of the island argue for different sources of drinking water. Copyright © 2012 John Wiley & Sons, Ltd.     

Periapical lesions and dental wear in the early Maori

Dental wear and intrabony lesions were evaluated in a sample of 225 skulls (136 male) of pre‐contact New Zealand Maoris. The degree and direction of surface wear was scored according to the method of Molnar ( Molnar 1971 . Human tooth wear, tooth function and cultural variability. American Journal of Physical Anthropology34: 175–190) and revealed severe surface loss in both males and females with horizontal wear being the dominant pattern (62.4% male, 57.5% female). The width of coronal tissue above the pulp chamber, as well as the maximum depth and width of periapical lesions, was measured from both standard radiographs and digital images. The high prevalence of periapical pathology in the Maori underlined the extreme nature of dental wear in these people. It is postulated that this degree of tooth loss may be attributable to a change in diet from large birds to marine‐dependence, the introduction of the kumara to New Zealand, dental erosion and finally, to the excessive masticatory forces exerted by a robust facial complex on normally sized teeth. Fenestrated lesions were highly prevalent (83% of skulls) and were centered mostly on the maxilla, with an even distribution among tooth classes. The finding of periapical lesions in teeth with minimal observable wear was attributed to traumatic occlusion. Copyright © 2001 John Wiley & Sons, Ltd.     

Evaluating microfossil content of dental calculus from Brazilian sambaquis

To date, limited numbers of dental calculus samples have been analyzed by researchers in diverse parts of the world. The combined analyses of these have provided some general guidelines for the analysis of calculus that is non-destructive to archaeological teeth. There is still a need for a quantitative study of large numbers of calculus samples to establish protocols, assess the level of contamination, evaluate the quantity of microfossils in dental calculus, and to compare analysis results with the literature concerning the biology of calculus formation. We analyzed dental calculus from 53 teeth from four Brazilian sambaquis. Sambaquis are the shell-mounds that were established prehistorically along the Brazilian coast. The analysis of sambaqui dental calculi shows that there are relatively high concentrations of microfossils (phytoliths and starch), mineral fragments, and charcoal in dental calculus. Mineral fragments and charcoal are possibly contaminants. The largest dental calculi have the lowest concentrations of microfossils. Biologically, this is explained by individual variation in calculus formation between people. Importantly, starch is ubiquitous in dental calculus. The starch and phytoliths show that certainly Dioscorea (yam) and Araucaria angustifolia (Paraná pine) were eaten by sambaqui people. Araceae (arum family), Ipomoea batatas (sweet potato) and Zea mays (maize) were probably in their diet.     

The Diet of Three Medieval Individuals from Caravate (Varese,Italy). Combined Results of ICP‐MS Analysis of Trace Elements and Phytolith Analysis Conducted on Their Dental Calculus

Teeth are in close contact with foodstuffs: phenomena such as caries and wear of the crowns are precious keys to gather information on diet and cooking techniques. Phytoliths are granules of hydrated amorphous silica, which are deposited in the oral cavity while chewing fruit and vegetables and incorporated into dental calculus during its formation. In diet investigation, different plants may therefore be related to specific phytolith shapes. Analysis of chemical trace elements, incorporated in dental calculus from ingested food and replacing small percentages of calcium in normal processes of resorption and redeposition, can further enrich the research on ancient diet. The aim of our work was to set up an analytical protocol, focalised on phytoliths and the inorganic elemental composition of dental calculus, on samples coming from medieval skeletons excavated in Caravate (Varese, Italy). The inductively coupled plasma mass spectrometry analytical technique proved to be particularly suitable for such studies. The results suggested a diet based both on the consumption of carbohydrates (dicotyledons and monocotyledons such as the Poaceae, also known as Gramineae; and Coniferae) and on proteins, mostly derived from fish. Copyright © 2015 John Wiley & Sons, Ltd.     

The dental arcade and human morphology

Cranio-dental fossils are relatively common in the fossil record and this paper considers the question of whether the dental arcade can act as a predictor of hominid morphology. A method of measuring the catenary number of an arcade is also described and defined. © 1998 John Wiley & Sons, Ltd.     

Recording dental caries in archaeological human remains

Dental caries is an important condition to record in archaeological collections, but the way in which recording is carried out has a large effect on the way in which the results can be interpreted. In living populations, dental caries is a disease that shows a strong relationship with age. Both the nature of carious lesions and their frequency change with successive age groups from childhood to elderly adulthood. There is also a progression in the particular teeth in the dentition which are most commonly affected and, in general, the molars and premolars are involved much more frequently than the canines and incisors. Lower teeth are usually affected more than upper, although the condition usually involves the right and left sides fairly equally. In the high tooth wear rate populations represented by many archaeological and museum collections, there is a complex relationship between the form of lesions and the state of wear, which adds yet another range of factors to the changing pattern of caries with increasing age. In the same populations, chipping, fracture and anomalous abrasion of teeth are also common, and these contribute similarly to the distribution and forms of carious lesion observed. Amongst the living, the pattern of ante‐mortem tooth loss is important in understanding caries and, in archaeological material, there is also the complicating factor of post‐mortem tooth loss. Finally, there is the question of diagnosis. There are diagnostic problems even in epidemiological studies of living patients and, for archaeological specimens, diagenetic change and the variable preservation of different parts of the dentition add further complications. For all these reasons, it is difficult to define any one general index of dental caries to represent the complete dentition of each individual, which would be universally suitable for studying a full range of collections from archaeological sites or museums. Variation in the nature of collections, their preservation, tooth wear, and ante‐mortem and post‐mortem tooth loss mean that when such a general index appears to differ between sites, there could be many other reasons for this, in addition to any genuine differences in caries incidence and pattern that might have been present. It is suggested here that the best approach is instead to make comparisons separately for each tooth type, age group, sex, lesion type and potential lesion site on the tooth. Copyright © 2001 John Wiley & Sons, Ltd.     

Starch granules,dental calculus and new perspectives on ancient diet

Recent work in various parts of the world has suggested the possibility of ancient starch granules surviving and adhering to archaeological artefacts. Often this information is used to infer aspects of diet. One additional source for recovery of archaeological starch granules is dental calculus. The presence of plant food debris in dental calculus is well known but has not been not widely investigated using archaeological material. The extraction of starch granules from dental calculus represents a direct link to the consumption of starchy food by humans or animals. Using dental calculus also sidesteps many other questions still inherent in using starch granules to reconstruct aspects of ancient diet, such as the effects of diagenesis on their morphology; as the starches are trapped inside a concreted matrix they are less likely to alter over time. We used amylase digestion by a starch-specific enzyme to confirm the material as starch.     

Dental health at the transition from the Late Antique to the early Medieval period on Croatia's eastern Adriatic coast

Dento‐alveolar pathologies: caries, ante mortem tooth loss, abscesses, calculus, alveolar resorption and tooth wear were analysed in two composite skeletal series from Croatia's eastern Adriatic coast (Dalmatia). The first consists of 103 skeletons from seven Late Antique (3rd–6th century AD) sites, the second of 151 skeletons from three Early Medieval (7th–11th centuries AD) sites. As recent bioarhaeological studies (Šlaus, 2008 ) showed a significant increase of disease loads and trauma frequencies in Dalmatia during the Early Medieval period, the aim of this study was to investigate whether dental health was equally adversely affected by the Late Antique/Early Medieval transition. The results of our analyses show that the frequencies of carious lesions, ante mortem tooth loss, abscesses and alveolar resorption increased significantly during the Early Medieval period, as did the degree of heavy occlusal wear on posterior teeth. These data suggest a change in alimentary habits, with a significantly higher dependence on carbohydrates and a greater reliance on hard, fibrous foods requiring vigorous mastication in the Early Medieval diet. The combination of higher calculus and lower caries rates in the Late Antique series similarly suggests more protein in the Late Antique diet and is, therefore, also consistent with the hypothesised change in alimentary habits. In general (the two exceptions are male caries and female alveolar resorption frequencies) lesion frequencies increased uniformly in both sexes suggesting that the deterioration of dental health during the Early Medieval period equally affected males and females. Cumulatively, the collected data suggest that the political, social, economic and religious changes that characterised the Late Antique/Early Medieval transition in Dalmatia resulted in a clear discontinuity, not only from the cultural, but also from the biological point of view with an evident deterioration of oral health during the Early Medieval period. Copyright © 2010 John Wiley & Sons, Ltd.     

Genetic relationships based on discrete dental traits: Basketmaker II and Mimbres

Investigations of the relationships between culture and biology among prehistoric populations of the southwestern United States can enhance our reconstruction of social interactions. The present study analyses the permanent dentition of Basketmaker II and Mimbres individuals from multiple sites using the Arizona State University dental anthropology system, and compares them with a regional sample. Discrete dental trait analysis provides a useful means for assessing the degree of genetic relatedness between populations when large comparative samples are available. Both in situ cultural change and population replacement have been posited as the dominant mechanism acting on Basketmaker and early Puebloan groups. We examine the relationship of these populations to probable Uto‐Aztecan speakers in light of the Uto‐Aztecan farmer migration hypothesis. The Basketmaker II complex, traditionally recognised as a distinct cultural unit, exhibits considerable population heterogeneity. Western Basketmakers appear to share biological ties with Uto‐Aztecan speakers, while Eastern Basketmakers do not, as predicted by the model. The relationship of the Mimbres to probable Uto‐Aztecan speakers is less clear because the Mimbres show only weak affinities to northern Mexican populations. Copyright © 2007 John Wiley & Sons, Ltd.     

Patterns of dental wear in the early Maori dentition

The levels of tooth wear were surveyed in 50 pre‐contact Maori skulls (23 of them female) from New Zealand. In addition to a generalized tooth wear index, we evaluated occlusal wear for erosion and abrasion. Occlusal microwear was also investigated using scanning electron microscopy (SEM) images and, finally, the occlusal slope was determined at the level of the first molar teeth. The general pattern of wear was one of severe occlusal reduction with no significant differences between males and females. Both silicone impressions and SEM views confirmed a large component (30%) of erosion. While most individuals had flat wear planes, 33% of males and 44% of females showed reversed Monson's curves. Accentuated Monson's curves were found in 23.8% of males. While these results confirm early studies of the excessive wear experienced by pre‐contact Maori, our study shows for the first time that erosion played a significant role in the dental wear of these people. Copyright © 2001 John Wiley & Sons, Ltd.     

Nonmetric dental characteristics of the early modern population of Okinawa Island in Nansei Islands,Japan

Dental morphological characteristics of the early modern population (c. 17–19th century AD) of Okinawa Island, Nansei Islands, were investigated to clarify their genealogical characteristics. We observed and classified 25 nonmetric traits of tooth crowns and roots from human remains (106 individuals) excavated from tombs of the early modern period in Okuma, Ginowan City, Okinawa. The incidences of these traits were compared with the incidences in the populations of the other Nansei Islands, of Japan, and of Asia overall. Univariate analysis of each trait and multivariate biological distance analysis based on the frequencies of the traits showed that the Okinawa population in the early modern period more closely resembled the migrant Yayoi populations than it did the native Jomon populations. It is difficult to support the “Ainu‐Ryukyu common origin theory” with regard to the early modern population in Okinawa without some modification of the theory, as well as the modern populations in Tanegashima and Okinawa Island. The geographical cline in the modern period from the northern Kyushu to the Okinawa Island via Tanegashima was confirmed in this analysis. Considering the major temporal changes in northern Kyushu and Tanegashima in addition to the geographical cline, the southward gene flow of the migrant Yayoi elements from northern Kyushu to the central Nansei Islands via the northeast end of the Nansei Islands is suggested. In addition, this study detected some temporal changes from the early modern to the modern period in Okinawa Island. Although the minor temporal change may be attributed to some genetic drift, gene flow from the Japanese main islands or China might be considered one of the causes of the change. Copyright © 2010 John Wiley & Sons, Ltd.     

Efficient but destructive: a test of the dental wash technique using secondary electron microscopy

The Boyadjian et al dental wash technique provides, in certain contexts, the only chance to analyze and quantify the use of plants by past populations and is therefore an important milestone for the reconstruction of paleodiet. With this paper we present recent investigations and results upon the influence of this method on teeth. A series of six teeth from a three thousand years old Brazilian shellmound (Jabuticabeira II) was examined before and after dental wash. The main focus was documenting the alteration of the surfaces and microstructures. The status of all teeth were documented using macrophotography, optical light microscopy, and atmospheric Secondary Electron Microscopy (aSEM) prior and after applying the dental wash technique. The comparison of pictures taken before and after dental wash showed the different degrees of variation and damage done to the teeth but, also, provided additional information about microstructures, which have not been visible before. Consequently we suggest that dental wash should only be carried out, if absolutely necessary, after dental pathology, dental morphology and microwear studies have been accomplished.