Hair surface and mechanical properties of Copt mummies from Antinopolis

In the late nineteenth century, excavations were conducted in the necropolis of the site of Antinopolis, a city founded in 130 CE. Over sixty Copt mummies, out of the thousands of graves excavated, were brought to France in the early twentieth century. Unlike mummies of the Pharaonic era, no specific study has been conducted on the mummies of the Christian era. To identify these techniques of mummification and their state of preservation, the Louvre museum has conducted a multidisciplinary study on thirty-nine mummies located throughout France.In this work, we studied the surface topography and the mechanical properties of the hair from eleven mummies.To qualify the hair wear, 3D topography of their surface has been measured with an interferometer of vertical resolution 2 nm and an analysed surface of 20 × 84 μm. The results show that, for most of the hair samples, the cuticle has completely disappeared and the cortex appears very porous. However, for a few of the hair samples, the scales are perfectly regular like those of healthy hair, which is probably due to the mummification techniques used.The mechanical properties of the hair were determined using a three-point bending mechanical test.The results show that the mechanical hair properties are not correlated with the surface morphology of the cuticle.     

Embalming methods and plants in Renaissance Italy: two artificial mummies from Siena (central Italy)

Knowledge of the embalming methods used in Renaissance Italy comes not only from the literary texts of physicians and surgeons of that period, but also from artificial mummies which have been found. In 1999 two mummified bodies, dated back to the end of 15^th and beginning of the 16^th century, were recovered in a crypt of the hospital chapel of Santa Maria della Scala in Siena (central Italy). The individuals were identified as Salimbene Capacci (1433–1497), Rector of the hospital, and his wife, Margherita Sozzini (?-1511). The mummies were submitted to autopsy and paleopathological examination, which included Computed Tomography. Palaeobotanical and palynological analyses were performed on the vegetable materials and plants used to fill the body cavities. This study was carried out with a multidisciplinary perspective, that allowed us to reconstruct the embalming techniques and substances used for mummification. The results are compared with the evidences provided by other mummies and embalming handbooks of that time.     

CT imaging of human mummies: a critical review of the literature (1979–2005)

The number of computerised tomography (CT) investigations of mummies has increased since the first published study in 1979. However, this approach has never been validated. We present a critical analysis of the literature (1979 to 2005). Relevant articles were selected via a MedLine search and analysed according to CT technique, methodology, and author's speciality. Thirty‐one original articles matched our selection criteria. Of these studies, 42% were authored by radiologists, while 26% had no contribution from radiologists. Hypothesis‐driven papers comprised only 9.7% of the total. While 84% of the studies had a stated purpose for conducting the CT study, only 67% of studies defined their CT protocol clearly. CT was used to study mummification techniques in 74% of instances, and/or to detect disease in 58%. Conclusions based on CT analysis were derived in 84% of studies, but only 32% of these answered specific questions. Furthermore, only 36% of these conclusions were related to the stated purpose of the study. Using the criteria of the grading system we developed, we found that 61% of studies were supported only by weak evidence. We conclude that evidence‐based research with better design should be encouraged in future palaeoradiological studies. Copyright © 2008 John Wiley & Sons, Ltd.     

Sources of mummy bitumen in ancient Egypt and Palestine

Bitumen used as a preservative in ancient Egyptian mummies was previously thought to come only from the Dead Sea in Palestine. Other, closer sources of bitumen were investigated at Abu Durba and Gebel Zeit on the shores of Egypt’s Gulf of Suez. Bitumen from these localities and from five mummies was analysed using molecular biomarkers derived from gas chromatography/mass spectrometry. It was found that four of the mummies contained Dead Sea bitumen, and the fifth and oldest (900 bc) had bitumen from Gebel Zeit, thus providing the first evidence for the use of an indigenous source of bitumen in ancient Egypt.     

Persistence and decay of the intestinal microbiota's DNA in glacier mummies from the Alps

The study of the DNA of ancient microorganisms in human remains represents one of the newest and most promising branches of molecular archaeoanthropology. Despite the growing number of papers addressing this subject, however, the analysis of ancient bacterial DNA is still a contentious issue. The indigenous microbiota of the gastrointestinal tract of man represents a community characterized by relative constancy in species composition and proportion. As a model system, we studied the preservation of the intestinal microbiota DNA in two naturally freeze-dried human mummies found on the Alps. This kind of mummy is an ideal subject for ancient DNA investigations. The first is a male body historically dated 1918 A.D. while the second is the famous Tyrolean Iceman (3.350–3.100 B.C.). The screening of bacterial 16S rRNA gene libraries from colon samples of the two mummies (49 clones for the 1918 mummy, 119 clones for the Iceman) showed that the characteristic composition of the intestinal microbiota of man (Alpha-, Beta-, Gammaproteobacteria, Bacteroides, Clostridia) is still kept in the library from the recent mummy while the Iceman's library is almost entirely composed by the DNA of clostridia. Comparison of the intestinal data with those from the literature describing the screening of 16S rRNA gene libraries from other parts of the Iceman's body and from permafrost specimens indicates that the changes in library composition may partly be attributed to the proliferation of clostridia inside the corpses, as described in forensic literature, and partly to the differential persistence of the DNA of gram-negative bacteria and endospore-former low-GC gram-positive bacteria. The present results contribute to the issue of the authentication of claims of pathogen DNA identification in archaeological human remains.     

Ultramicroscopic investigation of the preservation status of hair collected from a full‐term,intrauterine baby mummy of the Joseon Dynasty,Korea

Our previous studies on the ultramicroscopic structure of mummy hair from Joseon Dynasty (1392–1910) tombs sealed with a lime‐soil mixture barrier (LSMB) have provided initial, basic information on their preservation status. Numerous additional cases of various conditions are required in order to provide data sufficient for establishing Korean mummy preservation patterns on a firm basis, however, and so we performed electron microscopic studies on hair taken from a full‐term, intrauterine baby mummy found in Paju, Korea. The baby mummy was found within the uterus of a 16th‐century mummified woman aged 20–30 years old. Since the labour and delivery stage for this case was 2, and the uterus was found to be ruptured, the cause of death of both the mother and the baby was likely to have been hypovolemic shock occurring during labour. In scanning electron microscopic (SEM) and transmission electron microscopic (TEM) studies, we found that the surface of the hair of the baby mummy was coated with crystalline substances, mainly on the side facing the vaginal orifice. We also observed well‐preserved cuticle, cortex and medullar layers, completely preserved cuticle‐layer scales, as well as macrofibrils and melanin granules evenly spread within the cuticle and cortex layers. Because studies on infant or subadult mummies are very few, and since they have focused mainly on the palaeopathological aspects of those mummies, the preservation pattern of the hair of a full‐term baby mummy could contribute significantly to our knowledge of mummies from around the world. Copyright © 2008 John Wiley & Sons, Ltd.     

ROMANO‐EGYPTIAN RED LEAD PIGMENT: A SUBSIDIARY COMMODITY OF SPANISH SILVER MINING AND REFINEMENT*

Samples of red pigment from a group of seven Roman‐period Egyptian mummies, known as red‐shroud mummies, are investigated. Elemental analysis by inductively coupled plasma time‐of‐flight mass spectrometry (ICP–TOFMS) shows that the samples contain mostly Pb (83–92% by weight), along with 0.2–2.0% Sn. All of the samples are found to have similar trace element distributions when normalized to the continental crust, suggesting that they share a common geological origin. Lead isotope ratios are found to match the mixed lead sources typically associated with Rio Tinto, Spain – a site extensively mined for silver during the first century ad . Raman microspectroscopy identifies the major phase of each sample to be red lead (Pb₃O₄) with a minor phase of lead tin oxide (Pb₂SnO₄). Lead tin oxide does not occur naturally, and its incidental occurrence within the sample indicates that the material was heated under oxidative conditions at temperatures in excess of 650°C. In archaeological contexts, the high‐temperature oxidative treatment of lead is typically associated with metallurgical refinement processes such as cupellation. Based on this evidence, it is argued that the pigment was produced out of litharge associated with silver cupellation at the Rio Tinto site.     

Cats of the Pharaohs: Genetic Comparison of Egyptian Cat Mummies to their Feline Contemporaries

The ancient Egyptians mummified an abundance of cats during the Late Period (664 - 332 BC). The overlapping morphology and sizes of developing wildcats and domestic cats confounds the identity of mummified cat species. Genetic analyses should support mummy identification and was conducted on two long bones and a mandible of three cats that were mummified by the ancient Egyptians. The mummy DNA was extracted in a dedicated ancient DNA laboratory at the University of California - Davis, then directly sequencing between 246 and 402 bp of the mtDNA control region from each bone. When compared to a dataset of wildcats (Felis silvestris silvestris, F. s. tristrami, and F. chaus) as well as a previously published worldwide dataset of modern domestic cat samples, including Egypt, the DNA evidence suggests the three mummies represent common contemporary domestic cat mitotypes prevalent in modern Egypt and the Middle East. Divergence estimates date the origin of the mummies' mitotypes to between two and 7.5 thousand years prior to their mummification, likely prior to or during Egyptian Predyanstic and Early Dynastic Periods. These data are the first genetic evidence supporting that the ancient Egyptians used domesticated cats, F. s. catus, for votive mummies, and likely implies cats were domesticated prior to extensive mummification of cats.     

Fjord fishing in Mesolithic Western Norway

Recent excavations at two rockshelters (Olsteinhelleren and Sævarhelleren) on the Hardanger fjord in Western Norway have provided an unparalleled opportunity to examine the Mesolithic subsistence economy of this region. Thousands of fish remains (as well as numerous mammal and bird bones) have been analysed from these assemblages. Results show that the fishery was dominated by gadids, but labrids and salmonids were also important. Many other fish were present in small quantities, including the first specimen of sturgeon from the Stone Age of Norway. The transition to a more specialised fishery at the younger site, Olsteinhelleren, may be a reflection of a switch to the use of this locality as a logistic camp for the targeting of gadid fish.     

CHARACTERIZING THE MICROMORPHOLOGY OF SEDIMENTS ASSOCIATED WITH CHINCHORRO MUMMIFICATION IN ARICA,CHILE USING SEM AND EDS

We use scanning electron microscopy (SEM) and energy‐dispersive spectroscopy (EDS) to analyse clay sediments used during the mummification process of Chinchorro mummies from northern Chile. The significance of this study is that no prior work has characterized the sediment used by the Chinchorro during mortuary preparation and this approach offers a necessary preliminary step towards identifying modern equivalents for the materials used by the Chinchorro. Understanding the unique characteristics of this material may also help understand why they are so well preserved and aid in the future curation of Chinchorro mummies.     

Nicotine in the hair of mummies from San Pedro de Atacama (Northern Chile)

The consumption of plant-derived hallucinogenic substances through smoking and snuffing is a long-standing tradition in the south-central Andes. Chemical and archaeobotanical evidence point to the consumption of nicotine and tryptamine alkaloids in Northwestern Argentina and of tryptamine alkaloids in San Pedro de Atacama (SPA), in prehispanic times. In this paper, results are reported of gas chromatography–mass spectrometry (GC/MS) analyses aimed at identifying nicotine and tryptamine alkaloids in the hair of mummies from different cultural periods of SPA. Fifty-six samples were examined. While tryptamines were not found in any of the samples, nicotine was found in 35 samples, assigned to the Late Formative (1 of 1 sample from this period), Late Formative or Middle (1 of 2 samples from either of these periods), Middle (4 of 6 samples from this period) and Late Intermediate periods (8 of 12 samples from this period), or without assignment to period due to lack of contextual information (21 of 35 samples unassigned to a period). These results show a continuous consumption of nicotine from the Late Formative to the Late Intermediate periods of SPA (ca. 100 B.C.–1450 A.D.). No associations were found between presence of nicotine in the hair of mummies and presence of snuffing trays or of other snuffing paraphernalia in the corresponding tomb; furthermore, neither the diversity of the funerary context, measured in terms of the number of types of objects, nor the presence of gemstone necklaces differed between tombs with mummies with or without nicotine in their hair. Overall, these results suggest that consumption of nicotine was performed by members of the society at large, irrespective of their social and wealth status.     

Foodstuff placement in ibis mummies and the role of viscera in embalming

Recent excavation of a mummified ibis, in whose bill were found numerous snails, and the 2010 radiological study of ibis mummies from Yale's Peabody Museum drew attention to the presence of bird foodstuffs intentionally placed inside mummified ibises following evisceration. The foodstuff packing in the Peabody's ibises was likely contained within the birds' own viscera. Radiographs of a hatchling ibis mummy at McGill's Redpath Museum demonstrated similar placement of foodstuffs within the eviscerated body cavity. This pattern of ibis evisceration, with the previously unreported practice of foodstuff packing, suggests the provision of an afterlife food source to the bird. These findings lend support to the idea that the viscera of ibises, and humans alike, were meant to continue their living function in the afterlife. Given that organs of digestion and respiration were specifically preserved and retained within, or in conjunction with, human mummies, the implication is that animals were also treated with similar respect and care, and the crucial role of the viscera was recognised.     

The origin of the Juch'uypampa Cave mummies: strontium isotope analysis of archaeological human remains from Bolivia

Previous analyses of strontium isotopes from human bone and teeth have identified diverse patterns of residential mobility in the South Central Andes during the Middle Horizon (AD 500–1100). During this time, the large polity of Tiwanaku exerted great influence over what are now southern Peru, northern Chile, northwestern Argentina and Bolivia. Recently, five naturally-mummified individuals were discovered in the cave of Juch'uypampa in the Pulacayo region of southern Bolivia. Although these individuals were buried with a number of fine Tiwanaku-style grave goods as well as non-Tiwanaku items, the burial site is isolated and does not conform to the pattern of large Tiwanaku-affiliated cemeteries and residential sites outside of the Lake Titicaca Basin. Strontium isotope analysis was performed on enamel from two adult men and bone from a third adult male in order to test the hypotheses that one or more of the males was from either the Tiwanaku heartland in the Lake Titicaca Basin, the Chilean oasis of San Pedro de Atacama, which contains a series of cemeteries with Tiwanaku-style grave goods, or the local area in which they were buried. Results show that two individuals likely spent their childhood in the local area where they were interred, while the third man probably spent at least the last twenty years of his life in that region before being buried there. This raises interesting questions regarding the nature of Tiwanaku influence in southern Bolivia and the relationship between the Juch'uypampa mummies and the Tiwanaku polity.     

A NEW DIRECT RADIOCARBON AMS DATE FOR AN UPPER PALAEOLITHIC HUMAN BONE FROM SIBERIA

A new AMS radiocarbon date has been obtained from a Siberian human bone sample taken directly from a cranial fragment. The specimen comes from the north‐east Siberian site of Pokrovka 2 (55⁰19′46.30″N, 92⁰26′48.80″E). The remains consist of the anterior portion of the cranium or the superior part of the face; that is, the forehead and the roof of the eye sockets. Sadly, indifferent features of the frontal bone do not allow a confident diagnosis of the sex of the individual. The evidence suggests, however, that the remains are probably those of a young adult (teenager) and an anatomically modern human, rather than a Neanderthal. Radiocarbon dating was undertaken at the Oxford Radiocarbon Accelerator Unit (ORAU). The radiocarbon age determination of the bone fragment, 27 740 ± 150 bp (OxA‐19850), is one of the earliest direct dates for a modern human from Siberia. The Pokrovka cranial fragment dates to the middle Upper Palaeolithic of Eurasia and is broadly contemporary with the human remains from the famous eastern European site of Sunghir. When the radiocarbon age is compared with the Cariaco Basin ¹⁴C data set and the Greenland oxygen isotope record of NGRIP, it corresponds with Greenland Interstadial 5, a warmer phase of the Last Glacial period, although this is a preliminary conclusion, and is based on climate records that may or may not be wholly synchronous between the two locations.     

New radiological approach for analysis and identification of foreign objects in ancient and historic mummies

Traditional x-ray images of mummies may reveal foreign objects lodged within the body or its wrappings, but can only give a vague idea of the material of which these objects consist. More precise information may be obtained by means of computed tomography which delivers not only a three-dimensional reconstruction of the object's shape, but also a measurement of radiodensity and a representation of the radiological structure.While the density values and structures of body tissues and substances are well documented in radiological publications, little data exist of material not usually found in the human body. The aim of this study is to analyse the radiological density and structure of a series of test objects, so that these may serve as a reference for comparison with potential foreign objects found in mummified remains. Value and limitations of this method are discussed and the practical application is demonstrated through four examples.     

黑龙江省海林市杨林南山旧石器遗址石器研究

本文以杨林南山遗址石制品为主要研究对象,通过对其岩性的鉴定、标本数据的测量、石制品的类型学分析,和与周边地区同时期旧石器考古学文化的比较,以此加深对该遗址文化内涵及特征的认识,并对张广才岭地区的旧石器文化面貌有一初步的了解,同时为探讨东北亚旧石器文化间的交流与传播提供了一批宝贵的资料。     

Occipitalization of the Atlas in Two Female Skeletons from Apollonia Pontica,Bulgaria

Occipitalization of the atlas was observed in two adult female skeletons from the Greek colonial site of Apollonia Pontica (5^th to 3^rd centuries BC), located on the Bulgarian Black Sea coast. Representing a rare congenital anomaly of the atlanto‐occipital junction, this condition has been documented in very few skeletal remains from Classical antiquity. Postmortem damage to one of the specimens prevented an evaluation of its clinical significance. The dimensions of the second specimen, however, suggest that the affected individual may have experienced some neurological symptoms associated with her condition. The burial of the two females in close proximity to one another raises the possibility that they may have been biologically related. Copyright © 2012 John Wiley & Sons, Ltd.     

A Review of the Evidence for Melanoma in Nine Inca Mummies

An often cited statement in the medical literature is that nine Inca mummies (c.400 bc ) had skin and osseous lesions from melanoma. However, a review of the pattern and type of osseous lesions exhibited by these individuals and the age of at least one of them indicate the data are inconsistent with a diagnosis of melanoma. Copyright © 2017 John Wiley & Sons, Ltd.     

Analysis and interpretation of flint toolmarks found on bones from West Tump long barrow,Gloucestershire

West Tump is a Neolithic chambered tomb originally excavated in 1880. The mound was found to contain a mixed skeletal assemblage, the majority of which was disarticulated. Between 2000 and 2001 material from the site including human bone was re‐examined with a portion of this project focused on taphonomic evidence observed on the human skeletal material. Amongst the factors selected for investigation were deliberate toolmarks. Three specimens were identified as exhibiting toolmarks. Analysis demonstrated that the observed features were cutmarks, consistent with the use of flint tools. However those on two of the specimens were found to be both post‐mortem and recent. The cutmarks observed on the remaining specimen, a right clavicle, were identified as having been inflicted during the peri‐mortem period and consistent with decapitation. British Neolithic mortuary practice has been the subject of ongoing debate since the earliest excavations of relevant sites, but until very recently few assemblages from such contexts have been re‐examined and much of the data in use has derived from sources of considerable age and questionable reliability. The selective removal of specific skeletal elements (particularly skulls) has repeatedly been described in the literature regarding the collective funerary monuments of this period. It has often been assumed that such practices took place after the soft tissues had decayed. One of the examples discussed, suggests this may not always have been the case and the potential significance of this finding is discussed in addition to a possible interpretation of the cutmarks seen on the remaining specimens. Copyright © 2004 John Wiley & Sons, Ltd.