The possible influence of post-harvest objectives on Cucurbita maxima subspecies maxima and subspecies andreana evolution under cultivation at the Argentinean Northwest: an archaeological example

This paper investigates the possible links between postharvest activities and methods of plant husbandry or management of Cucurbita maxima ssp. maxima and C. maxima ssp. andreana in the prehispanic Argentinean Northwest area. Microscopic methods were used to assess the micromorphological characteristics of modern specimens of South American Cucurbita and Lagenaria species to obtain diagnostic anatomical traits. These traits were then used as criteria for identifying archaeological Cucurbitaceae rind remains from domestic to funerary contexts of the Pampa Grande archaeological site (1720 ± 50 bp, cal. 259–433 ad). Following the taxonomic identification of the archaeological plant remains, they were futher assessed for signs of human selection or possible cultivation, including: rind thickness, qualitative characters (lobbing, wartiness and colour) and postharvest traits (artificial shape, charring, staining and decoration of sherds). The results indicate the presence of Lagenaria siceraria together with spontaneous, intermediate and domestic C. maxima morphotypes. Different subspecies maxima morphtypes were recognized: those intended as food, having thin pericarps to facilitate consumption and those intended also as food, but as containers too, as in Lagenaria, in which the rinds are thickened and lignified. The latter morphotype may possibly represent a strategy of postharvest intensification, but not through new processing techniques, but through the development of landraces with a longer fruit shelf life, resulting from changing husbandry criteria to selective pressures over cultivated stands.     

High-resolution sclerochronological analysis of the bivalve mollusk Saxidomus gigantea from Alaska and British Columbia: techniques for revealing environmental archives and archaeological seasonality

The butter clam, Saxidomus gigantea, is one of the most commonly recovered bivalves from archaeological shell middens on the Pacific Coast of North America. This study presents the results of the sclerochronology of modern specimens of S. gigantea, collected monthly from Pender Island (British Columbia), and additional modern specimens from the Dundas Islands (BC) and Mink and Little Takli Islands (Alaska). The methods presented can be used as a template to interpret local environmental conditions and increase the precision of seasonality estimates in shellfish using sclerochronology and oxygen isotope analysis. This method can also identify, with a high degree of accuracy, the date of shell collection to the nearest fortnightly cycle, the time of day the shell was collected and the approximate tidal elevation (i.e., approx. water depth and distance from the shoreline) from which the shell was collected.Life-history traits of S. gigantea were analyzed to understand the timing of growth line formation, the duration of the growing season, the growth rate, and the reliability of annual increments. We also examine the influence of the tidal regime and freshwater mixing in estuarine locations and how these variables can affect both incremental structures and oxygen isotope values. The results of the sclerochronological analysis show that there is a latitudinal trend in shell growth that needs to be considered when using shells for seasonality studies.Oxygen isotope analysis reveals clear annual cycles with the most positive values corresponding to the annual winter growth lines, and the most negative values corresponding to high temperatures during the summer. Intra-annual increment widths demonstrate clear seasonal oscillations with broadest increments in summer and very narrow increments or no growth during the winter months. This study provides new insights into the biology, geochemistry and seasonal growth of S. gigantea, which are crucial for paleoclimate reconstructions and interpreting seasonality patterns of past human collection.     

Enamel Ultrastructure of Cattle from the Quaternary Period in India

Abstract

The present paper examines tooth enamel of three species of cattle, Bos acutifrons, Bos namadicus and Bos indicus, which are supposed to have been phylogenetically related and belong to the Quaternary period. It aims to show whether in a short geological time span of 2myrs, the changing environs that cattle were exposed to during the Early Holocene have caused any microstructural changes in their tooth enamel.

Mammalian teeth exhibit a very complex arrangement of prisms in the enamel. The prisms are bundles of hydroxyapatite crystallites, arrangement of which is genetically determined and subject to evolutionary change. Hypsodonty or high crowned molars are such examples where concomitant masticatory stress factors near the enamel-dentin junction (EDJ) are known to have affected and altered the enamel microstructure in several large mammalian genera. Modified radial enamel in the deep enamel layer in some ungulates, including cattle, is an adaptive response to these stress factors which appeared much earlier (Tertiary) in the evolutionary history of these large mammals.

The cattle enamel analysed here revealed several levels of structural complexity indicating its functional designations. The schmelzmuster in cattle are formed of three enamel types: radial enamel (outer enamel), Hunter-Schreger Bands (mid enamel), and modified radial enamel (inner enamel). There is an increase through time in the percentage of Hunter-Schreger Bands (HSB), which cover up to about 68% of the entire enamel surface among recent cattle. The modified radial enamel and radial enamel do have a receding trend in the Holocene, compared to their Pleistocene ancestors.     

CHARACTERIZATION OF 18TH‐CENTURY MEISSEN PORCELAIN USING SEM–EDS*

Analytical investigations of fragmented Meissen porcelains well dated to between 1725 and 1763 have been carried out using SEM–EDS. The aim of the analysis was to characterize elements in bodies, glazes and overglaze enamels in order to create a baseline data set of materials used in the manufacture of porcelain at the Meissen factory, Saxony, during the 18th century. Analyses indicate body and glaze compositions to be consistent with a post c.1720–30 date, when a change from a lime‐rich flux to a potassium‐rich flux is documented to have occurred. Overglaze enamel compositions are shown to be consistent with contemporary accounts of enamel preparations at Meissen. Overglaze enamel compositions show that mixtures of pigments, in addition to documented enamel 18th‐century preparations, were in use at Meissen between c. 1725 and 1763.     

Reconstructing dietary and environmental history from enamel isotopic analysis: time resolution of intra‐tooth sequential sampling

The carbon and oxygen isotopic composition of the mineral phase of tooth enamel is linked to diet and environment. Enamel is not remodelled once formed. Several studies of intra‐tooth isotopic variability in hypsodont mammal teeth have involved sequential sampling of enamel in order to detect changes in diet and environment during tooth formation. The possibility of exploring individual history opens the door to many applications, particularly in palaeoenvironment and herd management reconstruction. However, previous histological investigations have shown that enamel mineralization is a progressive and discontinuous process. The goal of the present study is to determine if this pattern significantly influences the time resolution of an intra‐tooth sequential sampling. Isotopic analyses (δ¹³C) were performed on tooth enamel from steers (Bos taurus) that had changed from a C₃ plant‐based diet to a C₄ plant‐based diet with very different carbon isotopic compositions. The change of diet was reflected in the mineral phase of enamel. The pattern of intra‐tooth isotopic variation suggests that completion of enamel mineralization required six to seven months. Such a lag in enamel mineralization will decrease the time resolution of enamel sequential sampling. The effects of prolonged mineralization of enamel have to be considered when interpreting patterns of intra‐tooth isotopic variations. Copyright © 2002 John Wiley & Sons, Ltd.     

Addressing data comparability in the creation of combined data sets of bioapatite carbon and oxygen isotopic compositions

Before amalgamating published isotope data, comparability should be demonstrated. This paper compares carbon and oxygen isotopic compositions of 30 enamel samples measured by two laboratories. The aims were to see what, if any, isotopic variation was observed, to determine the causes as needed and to correct if possible. Bioapatite was acidified at 90°C in 2006 and at 26°C in 2017, while δ values were corrected via one‐point normalization in 2006 and by two‐point normalization in 2017. One case (of the 30) produced different δ values between the analysis dates, suggesting contamination. Repeated carbon isotope ratio measurements were not meaningfully different. Repeated oxygen isotope ratio measurements were significantly different, even following correction for acid‐carbonate fractionation at different temperatures and the renormalization of 2017 δ values using one point; however, differences were not meaningful for interpretations. Results were used to calculate real interpretative differences (RIDs) for comparing enamel bioapatite as 0.6‰ for δ¹³C values and as 1.6‰ for δ¹⁸O values.     

Preservation assessment of Miocene–Pliocene tooth enamel from Tugen Hills (Kenyan Rift Valley) through FTIR,chemical and stable-isotope analyses

We investigated fossil tooth enamel of mammals and crocodiles from two Mio-Pliocene East-African formations (Lukeino and Mabaget Fms) using infrared spectroscopy and chemical and stable-isotope analyses. Infrared spectra indicate that the fossil enamel contains biological apatite (bioapatite), without significant secondary carbonate contaminations. Several empirical infrared indexes were used to analyze the crystal–chemical characteristics of enamel. Fossil enamel has less organic matter, water and structural carbonate of apatite than modern enamel with which it was compared. Fossil apatite has a better crystallinity than bioapatite. The calcium/phosphorus mass ratio and the fluorine content of fossil apatite show intermediate values between bioapatite and geological fluorapatite. The samples also display significant crystal-chemical variations, depending on the vertebrate group (mammals vs. reptiles) and the taphonomic context (Lukeino Fm vs. Mabaget Fm). In spite of these changes, no relationship was observed between the chemical contents (carbonate and fluorine) and the stable-isotope ratios of carbonate (δ¹³C and δ¹⁸O) in fossil enamel. Preservation of the palaeoenvironmental signals is strongly supported by the fact that the stable-isotope composition of the three investigated fossil mammalian taxa (deinotheres, equids and hippos) is consistent with their ecological features. For instance, typical C₃- and C₄-plant isotope compositions are reflected in the deinotheres and equids, respectively, and amphibious hippos display lower δ¹⁸O values than terrestrial herbivores, as expected.     

The craniology and relationships of four species of bos 1. Basic craniology: Bos taurus L. and its absolute size

This paper is the first of a series on the craniology of four species of Bos. Here (and in the next paper) Bos taurus in Britain is used to establish a basic craniology for the genus. In the initial sections notes are made on the nomenclature, material available, history, and age assessment of B. taurus. The subsequent section deals with the effects of sex, age and breed on absolute size.The results show that Bos taurus is very variable, particularly in the length of the horncore. Sex influences certain dimensions very greatly, particularly those of breadth. Although the horns of cows are often longer than those of bulls of the same breed the horncores may be shorter in cows. In studying the effects of age it can be seen that growth rates are initially very high, slowing down at about three years of age. Tooth row length decreases with age and hence it is a particularly unreliable indicator of size. Obviously breed affects size and it has been possible to divide the skulls studied into size groups which correspond well with known body weights.     

GEOGRAPHICAL PATTERNS IN BIOLOGICALLY AVAILABLE STRONTIUM,CARBON AND OXYGEN ISOTOPE SIGNATURES IN PREHISTORIC SW GERMANY*

In order to interpret strontium and oxygen isotope values in Neolithic human skeletons analysed previously, we begin to map the biologically available strontium, carbon and oxygen isotopic signatures of prehistoric southern Germany by analysing tooth enamel of pigs from archaeological sites distributed around the region. The mapping shows a marked upland–lowland difference in biologically available ⁸⁷ Sr/ ⁸⁶ Sr values, ranging between 0.7086 and 0.7103 in the sedimentary lowlands, and from 0.710 to as high as 0.722 in the crystalline uplands of the Odenwald, the Black Forest and the Bavarian Forest. In addition, carbon isotopes in the carbonate fraction of pig enamel were generally about 1–2 more enriched in ¹³ C in the uplands. Despite the expected depletion of ¹⁸ O with altitude, oxygen isotopes in pig enamel showed little correlation with site altitude, although for pig samples not older than the Iron Age there was some geographical correlation withδ¹⁸O patterns in modern precipitation.     

INVESTIGATING SEASONALITY AND SEASON OF BIRTH IN PAST HERDS: A REFERENCE SET OF SHEEP ENAMEL STABLE OXYGEN ISOTOPE RATIOS

Intra‐tooth sequential analysis of enamel δ¹⁸O is currently used to investigate birth seasonality in past animal populations, offering new insights into seasonal availability of animal resources, herd management and seasonality of site occupation. Reference data sets are still required to address two major difficulties: (1) that inter‐individual variability in the record of the seasonal cycle is affected by tooth size; and (2) that the season of birth cannot be directly estimated from the timing of tooth growth, because of a delay in enamel mineralization. We present a data set acquired on the lower second molar of 10 modern sheep from Rousay (Orkney) born within a few weeks of each other in April/May and submitted to the same environmental conditions until death. All sheep have recorded a sinusoidal pattern of δ¹⁸O variation spanning approximately a year. From the difference between the expected and the measured time sequence, the delay of enamel mineralization is estimated to be 5–6 months. The data set is then described using a model mainly based on a cosine function. The period, corresponding to the length of the M2 crown formed over a year, averaged 35.8 mm. A very slight variation of tooth growth rate with time and no attenuation of the isotopic signal towards the cervical margin of the crown could be detected in this data set. The lowest δ¹⁸O values, corresponding to the sheep's first winter, were tracked at a distance from the enamel/root junction that varied between 23.0 and 30.3 mm (x_min mean = 27.6 mm); the highest δ¹⁸O values, corresponding to the sheep's second summer, were between 6.3 and 11.6 mm (x_max mean = 9.9 mm). Most of the variability can be attributed to tooth size. When normalized on the period, x_min and x_max are 0.28 (± 0.05) and 0.78 (± 0.05) on average, meaning that the Rousay sheep have recorded the minimum and maximum δ¹⁸O values on average at 78% and 28%, respectively, of the end of the periodic cycle recorded in the second molar.     

Evidence for long-term averaging of strontium in bovine enamel using TIMS and LA-MC-ICP-MS strontium isotope intra-molar profiles

Abstract

High spatial resolution micro-sampling of tooth enamel offers the possibility of high temporal resolution isotope data to reconstruct climate, environment, diet and mobility. Questions remain about the duration and pattern of the maturation phase of enamel and the existence and direction of chronological 'time-lines'. LA-MC-ICP-MS measurements of c. 400 μm craters and TIMS analyses of transverse enamel sections of an archaeological bovine third molar were undertaken to investigate the long-term averaging of incorporated strontium. The same gradually increasing isotope profile was obtained from both approaches, indicating that the large increase in spatial resolution did not change the response profile obtained. The results suggest that even at the microscopic scale, strontium is incorporated over a period in excess of 12 months. Averaging of the input signal may result from both long-term retention of strontium in the skeleton and recirculation in the body pool, or long-term maturation of enamel on a microscopic scale. Whichever mechanism is responsible, it may not be possible to recover strontium isotope ratios with a high time resolution from cattle molar enamel unless there is a large imbalance in the amount of strontium supplied by different sources. Consequently, strontium isotope profiles may not be synchronous with those of lighter isotope systems.     

Problems and pitfalls in the diagnosis of linear enamel hypoplasia in cheek teeth of cattle

Prevalence and intensity of enamel hypoplasia have been used as markers of generalized physiological stress during dental development in a wide range of mammalian taxa. We studied cattle (Bos taurus) cheek teeth exhibiting morphological characteristics that are of relevance to the diagnosis of enamel hypoplasia in this and other bovid species. These characteristics were multiple, more or less horizontally arranged (waveform) lines or grooves in the cementum of the tooth crown and the adjacent root area, leading to an imbricated appearance of the cementum. On macroscopic examination of tooth surfaces, these lines resembled linear enamel hypoplasia (LEH). Microscopic analysis of tooth sections, however, revealed that the lines occurred in the cementum only, and that the underlying enamel did not exhibit morphological irregularities. In cheek teeth of older cattle, a thick cementum layer is regularly found in the cervical crown portion and the adjacent root area. Apposition of this cementum is related to the uplifting of the teeth from their alveoli, a process that compensates for the shortening of the tooth crowns due to occlusal wear. In the studied specimens, a pronounced periodic nature of tooth uplifting and the related deposition of cementum is the likely cause for the observed imbricated appearance of the cementum. While this phenomenon may be misinterpreted as representing a case of LEH, presence of enamel hypoplasia in bovid teeth may be overlooked when the defects become filled with coronal cementum and are therefore not apparent on external inspection. This was the case in one of the cattle teeth analyzed by us, in which the hypoplastic enamel defects were, however, clearly discernible in ground sections. Microscopic analysis of tooth sections is recommended for recording of LEH in bovid teeth in cases where macroscopic examination of tooth surfaces alone does not produce unequivocal results.     

Linear Enamel Hypoplasia and Age‐at‐Death at Medieval (11th–16th Centuries) St. Gregory's Priory and Cemetery,Canterbury, UK

Linear enamel hypoplasia (LEH) is a macroscopically detectable band‐like dental defect, which represents localized decrease in enamel thickness caused by some form of disruption to a child's health. Such dental deformations are utilized in osteoarchaeological research as permanent markers of childhood physiological stress and have been extensively studied in numerous ancient human populations. However, currently there is no such data for medieval populations from Canterbury, UK. Here, LEH is examined in the context of age‐at‐death in human burials from the medieval St. Gregory's Priory and adjacent cemetery (11^th–16^th centuries), Canterbury, UK. The cemetery and Priory burials represented lower (n = 30) and higher status (n = 19) social groups, respectively. Linear enamel hypoplastic defects were counted on mandibular and maxillary anterior permanent teeth (n = 374). The age and sex of each skeleton were estimated using standard methods. Differences in LEH counts, age‐at‐death, and LEH formation ages were sought between the two social groups. Results indicate significantly greater frequencies of LEH in the Cemetery (mean = 17.6) compared to the Priory (mean = 7.9; t = −3.03, df = 46, p = 0.002). Adult age‐at‐death was also significantly lower in the Cemetery (mean = 39.8 years) compared to the Priory burials (mean = 44.1 years; t = 2.275, df = 47, p = 0.013). Hypoplasia formation ages differed significantly between the Priory (mean = 2.49 years) and Cemetery (mean = 3.22 years; t = 2.076; df = 47; p = 0.034) individuals. Results indicate that childhood stress may reflect adult mortality in this sample, and that the wellbeing of individuals from diverse social backgrounds can be successfully assessed using LEH analyses. Results are discussed in terms of the multifactorial etiology of LEH, as well as weaning‐related LEH formation. Copyright © 2012 John Wiley & Sons, Ltd.     

Taphonomic analysis of remains of mammals eaten by pumas (Puma concolor Carnivora, Felidae) in central Argentina

Prey remains recovered from puma (Puma concolor) scat were analyzed in order to identify taphonomic features produced on them by this predator. Mammal remains are abundant in puma scat, but recognition of bone remains decreases as prey size increases. Modifications produced by digestion are strong. However, micromammal bones show a gradation in preservation that includes well preserved specimens. Data presented here and their interpretation may possibly be extrapolated to zooarchaeological or paleontological assemblage.     

Hyaline tissue of thermally unaltered conodont elements and the enamel of vertebrates

Comparison of the ultrastructure of the hyaline tissue of conodont elements and the enamel of vertebrates provides little support for a close phylogenetic relationship between conodonts and vertebrates. Transmission and scanning electron microscopy shows that the mineralised component of the hyaline tissue of Panderodus and of Cordylodus elements consists of large, flat, oblong crystals, arranged in layers that run parallel to the long axis of the conodont. Enamel in the dentition of a living vertebrate, the lungfish Neoceratodus forsteri, has crystals of calcium hydroxyapatite, arranged in layers, and extending in groups from the dentine-enamel junction; the crystals are slender, elongate spicules perpendicular to the surface of the tooth plate. Similar crystal arrangements to those of lungfish are found in other vertebrates, but none resembles the organisation of the hyaline tissue of conodont elements. The crystals of hydroxyapatite in conodont hyaline tissue are exceptionally large, perpendicular or parallel to the surface of the element, with no trace of prisms, unlike the protoprismatic radial crystallite enamel of fish teeth and scales, or the highly organised prismatic enamel of mammals.     

新石器时代广西地区橄榄属植物研究现状

新石器时代先民对植物资源利用一直是学界研究的热点问题。橄榄属植物是广西地区重要植物资源,然而有关橄榄属的系统研究十分缺乏。本文系统综述了国内外所发现的橄榄属遗存及其研究方法,进一步强调了拓宽识别方法对橄榄属遗存研究的重要性。本文对橄榄属遗存及其研究方法的梳理,不仅有助于理解新石器时代广西地区先民对橄榄属植物的利用情况,也为解读橄榄属植物在这一地区的驯化史、环境史提供重要信息。     

IN‐SITU ELEMENTAL AND Sr ISOTOPE INVESTIGATION OF HUMAN TOOTH ENAMEL BY LASER ABLATION‐(MC)‐ICP‐MS: SUCCESSES AND PITFALLS*

Trace element and Sr isotope data were obtained by laser ablation‐ and solution mode‐(MC)‐ICP‐MS analysis for tooth enamel from remains excavated at the New Kingdom period Egyptian colonial and Nubian cemetery site of Tombos (Sudan). Elemental abundances determined by both methods of ICP‐MS analysis yielded comparable values; however, ⁸⁷Sr/⁸⁶Sr values obtained by laser ablation were higher compared to their solution mode counterparts. This discrepancy is related to the production of a molecular interference—Ca + P + O (overlaps ⁸⁷Sr); hence the higher ⁸⁷Sr/⁸⁶Sr values recorded during ablation analyses. Laser ablation studies of enamel may provide relatively precise ⁸⁷Sr/⁸⁶Sr values rather quickly but cannot be used for accurately deciphering historical population migrations.     

Comparing the Chronological Distribution of Enamel Hypoplasia in Rogowo,Poland (2nd century ad) Using Two Methods of Defect Timing Estimation

The frequency and chronological distribution of enamel hypoplasias were assessed in a Roman Period population of the Wielbark culture from Rogowo, northern Poland, dated to the 2nd century ad . Hypoplasias were recorded on permanent incisors, canines, and premolars of 52 skeletons. The position of linear defects on the crown surface was measured and then converted to the age of occurrence using two methods: a conventional method that employs the chart of enamel development for the permanent teeth, constructed by Massler et al. (1941) in Swärdstedt's (1966) modification, and the method by Goodman & Song (1999), which corrects for hidden cuspal enamel. Hypoplastic defects were found in 48.1% of examined cases. Linear defects [linear enamel hypoplasias (LEH)] were observed in 38.5% of individuals and in 22% of investigated teeth. The chronological distribution of LEH according to the conventional method revealed two peaks of defects: one at 2.6–3.0 years of age and the other at 4.1–4.5 years of age. The method that accounts for hidden cuspal enamel also provided two peaks, but they occurred at later ages: 3.0–4.0 and 4.6–5.0 years of age. The prevalence of hypoplasia in the Rogowo population in comparison with other European populations of the Roman Period seems to be rather low for both individuals and teeth affected. This may indicate advantageous living conditions, which are supported by archaeological data that suggest general well‐being of the Wielbark people. Copyright © 2011 John Wiley & Sons, Ltd.     

MARINE RESOURCES IN THE MESOLITHIC AND NEOLITHIC AT THE GROTTA DELL’UZZO (SICILY): EVIDENCE FROM ISOTOPE ANALYSES OF MARINE SHELLS*

Oxygen isotopes in shell carbonate samples from the marine rocky‐shore intertidal gastropod Monodonta turbinata (Born) are investigated in both modern analogue specimens and in archaeological specimens from the Grotta dell’Uzzo (Sicily). Variations in shell edge values of δ¹⁸O in living specimens collected monthly over two years are closely correlated with monthly seawater temperatures measured at the time of collection, showing that the species can be used for palaeoseasonality studies. Analyses of shell edge δ¹⁸O values in archaeological specimens, from Mesolithic through to early Neolithic phases at the Grotta dell’Uzzo, enabled the inference of various seasons of collection of shellfish and how such seasonality varies between the different phases of occupation. Interesting similarities and differences exist between the seasons of marine shellfish exploitation and the seasons inferred from the vertebrate zooarchaeological assemblages. A major inference drawn from the analyses and discussion is that the exploitation of all marine resources (fish and shellfish) increased in the later Mesolithic and early Neolithic periods.     

Identifying food provenience using Sr/Ca ratio in human enamel

The strontium (Sr) concentration in bioapatite of human bone and teeth reflects the Sr concentration in food that is differentiated by two major factors: an abundance of Sr in local soils and the biopurification effect along the trophic levels. Eastern Syria is an area with a relatively high concentration of Sr within the Euphrates valley and a low concentration of Sr beyond the valley. For this reason, the strontium/calcium (Sr/Ca) ratio in human enamel retrieved from skeletons buried in cemeteries within the Euphrates valley may be used as a proxy for food provenience in a local population. A sample of human teeth (N = 72) from Tell Ashara and Tell Masaikh was divided into seven temporal subsets covering periods between c.2200 bce and the early 20th century ce . A significant difference in Sr/Ca values between temporal subsets is observed. The Sr/Ca values in later subsets are negatively correlated with δ¹³C values, indicating the wider exploitation of steppe areas outside the valley with more wild C4 grasses and a low Sr concentration. For periods with lower Sr/Ca values (c.2200–1900 bce and 19th–20th centuries ce ) there is additional textual and isotopic evidence for food transportation from outside the river valley, supporting the interpretation of Sr/Ca values as a proxy for food provenience in this specific area.