Animal palaeopathology: Possibilities and problems

A brief account of the range of pathological disorders affecting the skeleton and certain other tissues of animals (mainly mammals) is given to indicate a broad picture of the sort of lesions which might be encountered in archaeological contexts.Though palaeopathological bone specimens have been described since the 18th century, it is only recently that an interest in archaeozoological material has encouraged the tentative interpretation of such specimens. Despite the pessimism of some workers in the field, it has been shown that this material does persist in archaeological samples, and that while diagnosis is not always clear cut, some of the spectrum of bone changes induced by disease processes has been documented.As early as 4000 years ago an awareness of some of these diseases was recorded, with several early societies adopting preventive and therapeutic measures to deal with them. As advanced human communities came into more intimate contact with animals, transmission of infectious diseases was facilitated. Husbandry practices, too, are seen to be directly related to the incidence of certain conditions, the occurrence of which in archaeological material can yield an insight into animal keeping.Diverse data of archaeological significance is, in fact, retrievable not only from osteological remains, but from organic material which may be preserved under certain conditions. The impact of animal disease is far-reaching, with important implications not only for the economy but also for human health.     

A Possible Case of Hydrocephalus in a Medieval Child from Doonbought Fort,Co. Antrim,Northern Ireland

Hydrocephalus is a condition that is rarely found in the palaeopathological record. It has been demonstrated in a modern study of untreated cases of hydrocephalus that 50 per cent of children suffering from the disease die within the first 18 months of life. It is probable that the situation was the same in archaeological populations and that the delicate nature of neonatal and infant crania accounts for the paucity of palaeopathological evidence. As far as the author is aware there are approximately 30 possible cases of hydrocephalus known in the archaeological record throughout the world and only two of these cases originated in the British Isles. The following report presents evidence of a case of possible hydrocephalus in a 6–7-year-old juvenile from Northern Ireland. The skeleton was recovered from a post-thirteenth century context during excavations at the secular medieval fort of Doonbought, Co. Antrim. The diagnosis of hydrocephalus has been made on the basis of the abnormal and enlarged morphology and increased cranial capacity of the cranium. The only other palaeopathological lesion present in the remains was cribra orbitalia.     

Palaeo‐otology of cholesteatoma

A cholesteatoma is a collection of dead epithelial cells in the ear which becomes infected with low‐grade pathogenic microorganisms. The lesion tends to cause resorption of the surrounding bone, which potentially permits its recognition in skeletal remains. Cholesteatoma may occur in the middle or the outer ear. Using two examples of cholesteatoma in skeletal remains from British archaeological sites, the differential diagnosis of cholesteatoma from other aural diseases is demonstrated. Some of the differences in bony lesions caused by middle ear and external ear canal cholesteatoma which may enable the two to be distinguished in palaeopathological specimens are discussed. Attention is drawn towards the importance of making this distinction in ancient skeletal remains; middle ear and external ear canal cholesteatoma have a different array of causes and very different impacts upon health status. Copyright © 2005 John Wiley & Sons, Ltd.     

DNA FROM HUMAN ANCIENT BACTERIA: A NOVEL SOURCE OF GENETIC EVIDENCE FROM ARCHAEOLOGICAL DENTAL CALCULUS

We report a molecular methodology to obtain and analyse ancient bacterial DNA from archaeological dental calculus. Recent and archaeological DNA samples, as old as 4000 bp , were successfully extracted and amplified with species‐specific PCR primers. We propose this approach in order to: detect the presence of specific bacterial species infecting past human populations; compare the composition of ancient oral microbiomes among human populations; and analyse the genetic variability and covariation of bacteria and human host populations. Genomic analysis of bacteria from dental calculus is a promising source of evidence for palaeopathological and micro‐evolutionary studies, focused either on micro‐organisms or their human hosts.     

Limited yet informative: pathological alterations observed on human skeletal remains from third and second millennia bc collective burials in the United Arab Emirates

While syntheses of palaeopathological studies have been undertaken in many parts of the world, until recently little was known about human health in the past in the United Arab Emirates (UAE). Focusing on skeletal pathologies, this paper documents the results of the first synthetic palaeopathologlcal study for this country. The collections of archaeological human skeletal remains come from three sites dating to the third and two sites dating to the second millennia bc and consist predominantly of disarticulated, partially fragmented and often cremated remains. The paper initially discusses potential explanations for the neglected study of human skeletal remains from the UAE. This discussion is followed by an outline of the types of burial practices employed in the past in this region and their effects on preservation. The nature of the collections and the methodology employed in their study are described. Using specific headings, the pathological findings are then documented, followed by a discussion of the results of the skeletal analysis in light of the available archaeological evidence. Finally, it is shown that although limited by preservation, analyses of human skeletal remains provide valuable details about the types of diseases that affected people in the past in the UAE. While less detailed than some palaeopathological syntheses, the results presented in this paper lay the foundations for further work on humans in the past in eastern Arabia. Copyright © 2001 John Wiley & Sons, Ltd.     

Congenital Syphilis in the Archaeological Record: Diagnostic Insensitivity of Osseous Lesions

The paucity of convincing evidence for congenital bone lesions of syphilis in the archaeological record led to study of the human remains from the Buffalo site in West Virginia, dated at 550—650 years BP. The diagnosis of syphilis (venereal) in adults was based on previously validated population criteria for the recognition of syphilis and its distinction from among the other treponemal diseases. Among the 151 juveniles (23.3 per cent of the total series), only one had macroscopic evidence of periosteal disease. The low frequency of recognizable osseous stigmata characteristic of congenital syphilis, combined with the conspicuous absence of pathognomonic dental lesions, make such periosteal lesions insufficiently sensitive criteria for the identification of syphilis in the archaeological record. © 1997 by John Wiley & Sons, Ltd.     

Staining ground sections of natural dry bone tissue for microscopy

The histological staining of bone tissue is of great value in identifying structural changes in human bone tissue when examined microscopically, and is therefore routinely used by clinical pathologists. However, for archaeological and other types of undecalcified dry bone tissue, histochemical staining is currently not widely used. The purpose of this paper is to describe a new method developed for the staining of undecalcified archaeological bone which may be a valuable addition to the palaeopathological toolkit. Sections were ground by hand and stained with haematoxylin and eosin. The microstructure and architecture of the stained sections become clearly visible without compromising total tissue envisioning. In addition, staining enhances differentiation between taphonomically affected and non‐affected bone tissue. This paper accordingly describes a reproducible stepwise method for the production of ground stained sections. An additional troubleshooting paragraph discusses the most often encountered problems and provides solutions. Copyright © 2010 John Wiley & Sons, Ltd.     

A possible case of coccidioidomycosis from the Los Muertos site,Tempe, Arizona

Coccidioidomycosis is a fungal disease endemic to southwestern North America and parts of Central and South America. Coccidioidomycosis frequently disseminates to the human skeleton and produces mostly lytic skeletal lesions. However, this disease is infrequently described within archaeological populations. As a result, it is important to report potential cases in order to improve current understanding of the appearance and distribution of lesions resulting from coccidioidomycosis in archaeological specimens. This study describes skeletal lesions in an adult male recovered from the Los Muertos site, Tempe, Arizona (AD 500–1450). These lesions are present on the inferior border of the left scapular spine, the medial portion of the left first metacarpal head, and the medial portion of the right first metatarsal. The lesions are predominantly lytic with sclerotic and, in some cases, healed cortical bone distributed around their margins. Evidence of skeletal healing is recorded within the destructive focus of one lesion. Geographical information on pathogen endemism, overall lesion distribution, and agricultural‐era Hohokom behaviour suggest that coccidioidomycosis is the most likely diagnostic option for these lesions. Mounting evidence for possible infections in the palaeopathological literature, combined with high frequencies of nutritional stress levels in endemic regions, suggests that coccidioidomycosis contributed at least moderately to morbidity in the American Southwest. Copyright © 2006 John Wiley & Sons, Ltd.     

Avulsion injuries of vertebral endplates

Bone changes resulting from avulsions involving vertebral endplates have had little, if any, systematic attention in palaeopathological literature. To gain insight into their occurrence and into their variety, two archaeological skeletal collections covering the period AD 1455–1824 were examined. Additional skeletal material was used to illustrate typical examples. A quarter of the 44 adults who had adequate numbers of vertebrae at all spinal levels showed minor to major vertebral endplate changes as a result of avulsion injuries. The male/female ratio was 7:4. The ratio of individuals with injuries contracted during their youth and during their adulthood was 3:8 (n=11). Approximately half of the affected individuals showed such changes in multiple vertebrae. In about one third of the cases, concomitant vertebral fractures, which did not involve the endplates, were recorded. Since the existing classifications of endplate changes from avulsion injuries were developed on the basis of clinical diagnoses made by means of X‐ray or by autopsy, an adapted and extended outline for palaeopathological use is proposed. A series of differential diagnoses is discussed. Although, in general, the poor preservation of archaeological spines hampers epidemiology, the diagnosis of spinal avulsion injuries offers interesting information at the individual level. Copyright © 2000 John Wiley & Sons, Ltd.     

The identification of chronic ear disease in the dried skull

Ear disease, and in particular chronic ear infection, has received scant attention in the palaeopathological literature. This paper describes the chronic infection known as cholesteatoma and how it may be identified in the archaeological specimen. A survey is made of the incidence of the condition in a series of pre-dynastic Egyptian skulls.     

Flatfoot: the palaeopathological diagnosis

Bipedal human motion is related to the original shape of the foot. Distortion and degenerative changes may be caused by failure in the complex chain of movements. There are few references to valgus flatfoot in either the palaeopathological or medical literature. The study of three French medieval series (Macon, Larina and Cutry) in the osteological collections of the CEPAM (UMR 6130 – CNRS/UNSA) at Valbonne (France) enabled us to define several significant osseous signs which provide evidence of abnormal biomechanical constraints following structural change in the foot. The consequences of the change of axis of the talar pulley, abnormal osseous contacts, and evolutionary modifications noticed in some synostoses of the tarsus, are particularly useful for study. Analysis of the location of arthritic change in the joint may produce an additional coherent argument to the diagnosis. The recognition of flatfootedness in an archaeological context provides a marker of health and of distress, which is all the more interesting since bones required for this diagnosis are often present and relatively well preserved in the excavations of burials. Copyright © 2006 John Wiley & Sons, Ltd.     

Severe vertebral collapse in a juvenile from the graveyard (13th/14th–19th centuries) of the São Miguel church (Castelo Branco,Portugal): differential palaeopathological diagnosis

Diseases that culminate into vertebral collapse are of intricate diagnosis both in palaeopathology and modern clinical practice. When analysing human skeletal remains from the archaeological record this difficulty is amplified due to the absence of complementary medical diagnostic information. This is especially evident when the distinction between tuberculous and pyogenic spondylitis is intended. Taking into consideration this challenging task and based on the macroscopic and radiological study of the skeleton number 8, a specimen exhumed from the East necropolis (13th/14th to 19th centuries) of the São Miguel church, at the Portuguese city of Castelo Branco, the aims of the work here presented are to discuss the range of possible aetiological factors, especially infectious ones, ascribable to the striking pathological changes noticed on this 12‐year‐old individual. These included alterations on the axial skeleton, namely extensive vertebral destruction, presenting as a gibbus deformity, and correlated thoracic deformities. Consubstantiated on palaeopathological and clinical research, tuberculous spondylitis seems to be the most probable cause for the reported lesions. However, the scrutiny between this condition and other pyogenic spinal infections is of extreme complexity when analysing ancient human remains and deserves in‐depth future investigations. Within the framework of the Portuguese archaeological record, the specimen here presented is of major relevance since the pattern and severity of the spinal osseous changes observed were not previously reported. Further, if tuberculous spondylitis is assumed as the most probable diagnosis, the case here presented represents one of the earliest skeletal evidence of this condition in Portugal. Copyright © 2009 John Wiley & Sons, Ltd.     

A Critique of Faunal Analysis; Inconsistency among Experts in Blind Tests

Fish faunal specialists with doctoral degrees independently evaluated samples of fish bones, teeth, and scales from a coastal archaeological site in San Luis Obispo County, California (CA-SLO-165). The considerable variation in interpretation raises serious questions regarding the accuracy of reporting and the usefulness of the data generated. Little agreement at the species level, wide differences in the total numbers of specimens in a given taxon, apparently inaccurate identifications, placements of specimens in excessively specific or overly general categories were typical inconsistencies. Recommendations for minimizing problems include having faunal work done by a specialist with biological knowledge of the specific animal group, and geographic region, identifying the reference collection used in the evaluation, stipulation of the criteria used for distinguishing similar species, and the location of the storage facility for the sample. Second opinions should be solicited and unreviewed data appearing in the “grey literature” should not be cited. A vigorous review process, rather than the reader, should select for credible information.     

Age estimation by tooth/pulp ratio in canines by peri-apical X-rays: reliability in age determination of Spanish and Italian medieval skeletal remains

Estimation of age at death is an unavoidable step in the process of human identification, both in forensic practice and in the anthropological and palaeopathological study of skeletal remains. In several cases, in which medical or demographic records are completely lacking, a reliable estimation of the age at death becomes very important. Skeletal remains from archaeological contexts suffer from several biasing factors such as post-mortem changes, taphonomy and various burial practices depending on age, sex and social status of the deceased persons.     

Skeletal evidence for hyperparathyroidism in a 19th century child with rickets

The skeletal remains of a child aged 2.5–3.5 years, recovered during archaeological excavations at the churchyard of St Martin's Church, Birmingham, UK, were examined using gross observation, radiography and scanning electron microscopy. Lesions suggestive of the presence of rickets and of secondary hyperparathyroidism were found. This appears to be a first report of secondary hyperparathyroidism in response to rickets in a palaeopathological specimen. The potential of microscopic examination of bone for interpreting disease processes is emphasised. Copyright © 2006 John Wiley & Sons, Ltd.     

A Comparison of Radiological and Palaeopathological Diagnostic Criteria for Hyperostosis Frontalis Interna

Hyperostosis frontalis interna (HFI) is a common clinical finding in post-menopausal women, less often in men. The characteristic billowing symmetrical new bone formation is found on the endocranial surface of the skull and rarely causes any significant symptoms. In clinical medicine HFI is diagnosed from its X-ray appearance, whereas in palaeopathology it is diagnosed by direct observation of the skull. There are no standard palaeopathological criteria to diagnose HFI. In past populations HFI appears to be much less common than today, with modern prevalences of up to 70 per cent of women over 40 affected, compared with archaeological prevalences of between 1 and 4 per cent. This discrepancy has been attributed to a younger mean age at death in ancient populations. This study aims to test the hypothesis that the difference in prevalence may be due in part to the differential nature of diagnosis between the two disciplines. A sample of 85 skulls was X-rayed, and the presence of HFI was recorded using radiological criteria. Using this method the results showed a marked increase in the number of cases of HFI to 31 per cent in females in the ancient population. © 1997 by John Wiley & Sons, Ltd. Int. J. Osteoarchaeol., 7 : 157–164 (1997) No. of Figures: 4. No. of Tables: 3. No. of References: 13.     

Thalassaemia: Its diagnosis and interpretation in past skeletal populations

The diagnosis of thalassaemia in archaeological populations has long been hindered by a lack of pathogonomic features, and the non‐specific nature of cribra orbitalia and porotic hyperostosis. In fact, clinical research has highlighted more specific diagnostic criteria for thalassaemia major and intermedia based on changes to the thorax (‘rib‐within‐a‐rib’ and costal osteomas). A recent re‐examination of 364 child skeletons from Romano‐British Poundbury Camp, Dorset revealed children with general ‘wasting’ of the bones and three children who demonstrated a variety of severe lesions (e.g. zygomatic bone and rib hypertrophy, porotic hyperostosis, rib lesions, osteopenia and pitted diaphyseal shafts) that are inconsistent with dietary deficiency alone, and more consistent with a diagnosis of genetic anaemia. Two of these children displayed rib lesions typical of those seen in modern cases of thalassaemia. The children of Poundbury Camp represent the first cases of genetic anaemia identified in a British archaeological population. As thalassaemia is a condition strongly linked to Mediterranean communities, the presence of this condition in a child from England, found within a mausoleum, suggests that they were born to wealthy immigrant parents living in this small Roman settlement in Dorset. This paper explores the diagnostic criteria for genetic anaemia in the archaeological literature and what its presence in ancient populations can contribute to our knowledge of past human migration. Copyright © 2010 John Wiley & Sons, Ltd.     

Tuberculosis among Iron Age individuals from Tyva,South Siberia: palaeopathological and biomolecular findings

The study focuses on the evidence for tuberculosis apparent in an Iron Age population recovered from the cemetery of Aymyrlyg, Tyva (Tuva), South Siberia. A recent wholly molecular study of five of the cases confirmed the presence of Mycobacterium tuberculosis (MTB) complex DNA in four of the individuals. In all cases the disease was caused by strains of Mycobacterium bovis rather than Mycobacterium tuberculosis and represents the first positive identification of the bovine form of the disease in archaeological human remains. Details of the palaeopathological characteristics of the cases are provided in the current paper, while the molecular observations are extended to include a quantitative evaluation of the surviving mycobacterial DNA using real-time PCR. The observation that bovine tuberculosis was the pathogen responsible is discussed in terms of current understanding of the evolution of the MTB complex as well as the implications for future ancient DNA studies in this area.