Persistence and decay of the intestinal microbiota's DNA in glacier mummies from the Alps

The study of the DNA of ancient microorganisms in human remains represents one of the newest and most promising branches of molecular archaeoanthropology. Despite the growing number of papers addressing this subject, however, the analysis of ancient bacterial DNA is still a contentious issue. The indigenous microbiota of the gastrointestinal tract of man represents a community characterized by relative constancy in species composition and proportion. As a model system, we studied the preservation of the intestinal microbiota DNA in two naturally freeze-dried human mummies found on the Alps. This kind of mummy is an ideal subject for ancient DNA investigations. The first is a male body historically dated 1918 A.D. while the second is the famous Tyrolean Iceman (3.350–3.100 B.C.). The screening of bacterial 16S rRNA gene libraries from colon samples of the two mummies (49 clones for the 1918 mummy, 119 clones for the Iceman) showed that the characteristic composition of the intestinal microbiota of man (Alpha-, Beta-, Gammaproteobacteria, Bacteroides, Clostridia) is still kept in the library from the recent mummy while the Iceman's library is almost entirely composed by the DNA of clostridia. Comparison of the intestinal data with those from the literature describing the screening of 16S rRNA gene libraries from other parts of the Iceman's body and from permafrost specimens indicates that the changes in library composition may partly be attributed to the proliferation of clostridia inside the corpses, as described in forensic literature, and partly to the differential persistence of the DNA of gram-negative bacteria and endospore-former low-GC gram-positive bacteria. The present results contribute to the issue of the authentication of claims of pathogen DNA identification in archaeological human remains.     

Foodstuff placement in ibis mummies and the role of viscera in embalming

Recent excavation of a mummified ibis, in whose bill were found numerous snails, and the 2010 radiological study of ibis mummies from Yale's Peabody Museum drew attention to the presence of bird foodstuffs intentionally placed inside mummified ibises following evisceration. The foodstuff packing in the Peabody's ibises was likely contained within the birds' own viscera. Radiographs of a hatchling ibis mummy at McGill's Redpath Museum demonstrated similar placement of foodstuffs within the eviscerated body cavity. This pattern of ibis evisceration, with the previously unreported practice of foodstuff packing, suggests the provision of an afterlife food source to the bird. These findings lend support to the idea that the viscera of ibises, and humans alike, were meant to continue their living function in the afterlife. Given that organs of digestion and respiration were specifically preserved and retained within, or in conjunction with, human mummies, the implication is that animals were also treated with similar respect and care, and the crucial role of the viscera was recognised.     

Preservation of DNA in ancient Egyptian mummies

The presence of DNA has been demonstrated in the cell nuclei of an ancient Egyptian mummy fragment. When extracted, this DNA proved to be degraded to a considerable extent and chemically modified. However, the preservation of nucleic acids in this specimen suggests that applying recombinant DNA techniques to the study of ancient mummified tissues might prove to be a fruitful future area of research.     

Nicotine in the hair of mummies from San Pedro de Atacama (Northern Chile)

The consumption of plant-derived hallucinogenic substances through smoking and snuffing is a long-standing tradition in the south-central Andes. Chemical and archaeobotanical evidence point to the consumption of nicotine and tryptamine alkaloids in Northwestern Argentina and of tryptamine alkaloids in San Pedro de Atacama (SPA), in prehispanic times. In this paper, results are reported of gas chromatography–mass spectrometry (GC/MS) analyses aimed at identifying nicotine and tryptamine alkaloids in the hair of mummies from different cultural periods of SPA. Fifty-six samples were examined. While tryptamines were not found in any of the samples, nicotine was found in 35 samples, assigned to the Late Formative (1 of 1 sample from this period), Late Formative or Middle (1 of 2 samples from either of these periods), Middle (4 of 6 samples from this period) and Late Intermediate periods (8 of 12 samples from this period), or without assignment to period due to lack of contextual information (21 of 35 samples unassigned to a period). These results show a continuous consumption of nicotine from the Late Formative to the Late Intermediate periods of SPA (ca. 100 B.C.–1450 A.D.). No associations were found between presence of nicotine in the hair of mummies and presence of snuffing trays or of other snuffing paraphernalia in the corresponding tomb; furthermore, neither the diversity of the funerary context, measured in terms of the number of types of objects, nor the presence of gemstone necklaces differed between tombs with mummies with or without nicotine in their hair. Overall, these results suggest that consumption of nicotine was performed by members of the society at large, irrespective of their social and wealth status.     

Dating Danish textiles and skins from bog finds by means of C AMS

This study presents the results of 44 new ¹⁴C analyses of Danish Early Iron Age textiles and skins. Of 52 Danish bog finds containing skin and textile items, 30 are associated with bog bodies. Until now, only 18 of these have been dated. In this paper we add dates to the remaining finds. The results demonstrate that the Danish custom of depositing clothed bodies in a bog is centred to the centuries immediately before and at the beginning of the Common Era. Most of these bodies are carefully placed in the bog – wrapped or dressed in various textile and/or skin garments. The care with which these people were placed in the bog indicates that they represent a hitherto unrecognised burial custom supplementing the more common burial pratice for this period.     

Three-dimensional image of seismic refraction tomography and electrical resistivity tomography survey in the castle of Occhiolà (Sicily,Italy)

A geophysical survey was carried out at the ruins of Occhiolà Castle (Sicily, Italy), a medieval village located at the north-western part of a hill named “Terravecchia” at 491 m asl. In order to map the archaeological structures (such as walls and burrows), Electrical resistivity tomography (ERT) and seismic-refraction tomography methods were used. The resistivity variations are known to correlate quite well with the lithology, thus providing important information for identifying the buried archaeological remains. On the other hand, seismic velocity variations provide information about the geometrical features of the remains. The two geophysical methods are used in an enjoined way to better aid the interpretation and evaluate the significance and reliability of the results obtained with each single method. The electrical and seismic data are displayed in three dimensions by using the iso-resistivity and iso-velocity surfaces, respectively. This allows us to effectively define the location of the anomalies present in the single electrical and seismic sections. The results obtained in the survey highlight the presence of structures of regular shape, probably due to features of archaeological interest.