Preservation of bird bones: erosion versus digestion by owls

Surface damage to bird bones from pellets of three species of owls (S. aluco, A. otus and B. bubo) was compared with that of bird bones exposed to weathering and soil corrosion (Záskogo Cave in the Western Balkan Mountains, Bulgaria and Ciemna Cave, southern Poland). Scanning electron microscopy and light microscope examinations indicate that the effects of weathering of bird bones (extensive pitting and flaking away on whole bones, holes with sharp edges, depressions with rough bottoms and sharp edges of breakage) are distinguishable from those of digestion (rounding of hole edges on articular ends and sometimes on shafts, rounding of breakage). However, soil corrosion may produce similar rounding to that of digestion, which may hamper taphonomic interpretations of fossil assemblages. Two stages of weathering in bird bone are distinguished. Generally, the kind of damage done to bird bones is similar to that done to mammalian remains. Taphonomic studies of fossil bird remains should take into account a combination of features, including surface damage, fragmentation and possible chemical alterations of bone tissues. © 1997 John Wiley & Sons, Ltd.     

Bone diagenesis in the European Holocene II: taphonomic and environmental considerations

We have applied cluster analysis to mercury intrusion porosimetry data from 219 archaeological bones (121 human and 98 animal) and soil chemistry data from 219 accompanying soil samples (1 per bone sample), to investigate the influence of soil chemistry on bone preservation. The samples chosen for the study were obtained from sites ranging in time from the pre-modern to the Mesolithic and were representative of burial environments across Europe (from the Baltic to the Mediterranean). These results represent the single largest database for archaeological bone preservation in the European Holocene to date and demonstrate the potential for large-scale diagenetic studies to help develop long term preservation strategies for our European heritage. Despite the variety of sites and environments, bones could be categorised into only four main diagenetic types. Furthermore, soil chemistry appears to significantly affect only one type of preservation, the pathway characterised by loss of mineral. In neutral to basic soils, taphonomy and in particular the differences between the treatment of human and animal remains, becomes the dominating factor in determining preservation. Using these results, strategies for heritage management of archaeological sites can be suggested; grouping sites into those requiring immediate excavation and those where in situ preservation is viable.     

Bone Diagenesis and Taphonomic History of the Paso Otero 1 Bone Bed, Pampas of Argentina

Samples of guanaco bone from an archaeological site in the Pampas of Argentina have been analysed to understand the diagenetic profile of the bone assemblages that characterized the taphonomic history of the site. Two archaeological occupations of Paso Otero 1 were investigated, encompassing similar landscape settings, climates, and depositional environments. The time span is a c. 2000 year period from c. 4800 to 2800 years . A total of 30 bone samples taken from both occupations were used to provide a preliminary characterization of the diagenetic pathways at the site. The parameters investigated provide a comprehensive account of how both mineral (hydroxyapatite) and bone protein (collagen) have been altered. In order to compare the two bone assemblages in terms of their diagenetic parameters, multivariate analyses were conducted. Results indicate two different diagenetic profiles in the site, % N being one of the variables that accounts for most of the variation in Paso Otero 1. The diagenetic analyses indicate that protein is less preserved in the bone assemblage from the middle stable landscape. Alternative interpretations of the diagenetic profiles are discussed in light of the taphonomic history of the site, and palaeoenvironmental information of the region. One hypothesis stresses the importance of the role of climate in defining the different diagenetic pathways, and the other the continued action of the combined diagenetic factors along time as the main explanation for the variability in the state of preservation of the bones in Paso Otero 1.     

Patterns of Diagenesis in Bone II: Effects of Acetic Acid Treatment and the Removal of Diagenetic CO3

Archaeological bones of varying preservation have been treated with 0·1 M acetic acid in order to investigate the effect on structural and chemical alterations caused by diagenesis. Acetic acid is commonly used as a “cleaning agent” for removing diagenetic carbonate from bone and enamel, in an attempt to recover original, biogenic signals for use in dietary and¹⁴C dating studies.Diagenetic parameters were measured before and after treatment on a range of archaeological bones with good and bad preservation. Histological preservation defined the behaviour of the correlating parameters, where correlation coefficients between carbonate content and crystallinity, microporosity and macroporosity increased significantly after treatment. For histologically well preserved material, acetic acid is effective at returning carbonate content to around that of modern bone. Where bone is extensively damaged by micro-organisms, “loose” diagenetic material can be removed, but a fraction largely composed of hypermineralized bioapatite remains, which, we believe, cannot be reliably used to obtain accurate biological signals.     

Bone Modifications Resulting from Captive Chimpanzee Mastication: Implications for the Interpretation of Pliocene Archaeological Faunas

In order to obtain chimpanzee (Pan troglodytes) mastication damage on bones, cleaned, disarticulated ribs and long bones of bovids and cervids were coated with food substances found palatable by captive chimpanzees. The bones were then presented to four groups of mixed sex and mixed age chimpanzees from the Tulsa Zoo (Oklahoma) and the Southwest Foundation for Biomedical Research (San Antonio, Texas). In the process of feeding on the coated bones, the chimpanzees inflicted discernible mastication damage on 73·61% of the total recovered bone specimens. Analysis of these chimpanzee-induced bone modifications reveals that non-technological hominoids of the same approximate size and with comparable dentition and bite force to the gracile australopithecines and earliestHomoare very capable of inflicting the same range and degree of damage to bones as are feeding carnivores. This finding implies that zooarchaeologists must take a contextual (configurational) approach when analysing mastication damage on arch-aeological bones, rather than automatically attributing all such damage to carnivores.     

Dry-bone manifestations of rickets in infancy and early childhood

Rickets and osteomalacia are the subadult and adult expressions of a disease in which the underlying problem is a failure to mineralize bone protein (osteoid). The most common cause of this disease is a physiological deficiency in vitamin D. The associated problems include deformed bones and this condition is well known in pre-modern medical texts and documents as a fairly common cause of morbidity. Given these facts, it is surprising that the literature on palaeopathology provides very little evidence of this disease in archaeological human skeletal samples. The medieval sample (N=687) of human remains from Wharram Percy, North Yorkshire, England contains a remarkable subsample of eight burials in which a spectrum of pathological features is expressed. The subsample includes infants ranging in age from 3 to 18 months at the time of death. Ten abnormal bone features were identified in the subsample, including: (i) cranial vault porosity; (ii) orbital roof porosity; (iii) deformation of the mandibular ramus; (iv) deformation of arm bones; (v) deformation of leg bones; (vi) flared costo-chondral ends of ribs; (vii) irregular and porous cortex of the costo-chondral ends of the ribs; (viii) abnormality of the growth plates of long bones; (ix) irregular and porous surfaces of the metaphyseal cortex; (x) thickening of the long bones, particularly in the metaphyseal areas. Not all of these features were found in all of the cases. Nevertheless, the overall pattern of skeletal abnormality fits well with the anatomical and radiological conditions associated with rickets. © 1998 John Wiley & Sons, Ltd.     

Micro-sampling of human bones for mobility studies: diagenetic impacts and potentials for elemental and isotopic research

The nature of long bone formation and the pathways of interaction between bone samples and the burial environment suggest that portions of the bones disconnected from the arterial system are resistant to diagenetic alteration. Preliminary work on femurs from Early Bronze Age hunter-gatherers in Cis-Baikal, Siberia shows that the nature and progression of chemical changes in the bone matrix due to microbial attack can be analyzed using laser ablation inductively coupled plasma mass spectrometry. Intra-osteon variability in elemental concentrations and strontium isotope ratios (⁸⁷Sr/⁸⁶Sr) indicate the presence of unaltered portions of bone within diagenetically modified bone and suggest that useful data remain accessible. These biogenic signals can potentially be useful for mobility research in broad terms and the smaller timescales within an individual's lifetime (months, years), accessible therein. Laser ablation micro-sampling of femur specimens showed that intra-osteon elemental composition of Ba, Re, and Cs varied within and was correlated between multiple osteons of a single bone. Portions of chemically unaffected bone were identified within, and effectively discriminated from diagenetically altered bone tissue. Areas showing visual alterations and erratic or uncorrelated Ca and Sr elemental results also had anomalous Sr isotope ratios, suggesting diagenetic alteration in those places. Compositional and isotopic analysis of intact portions of bone supports the hypothesis that hunter-gatherer groups in Cis-Baikal made numerous major movements during their lives. Microscopic analysis of long bones clarifies aspects of biodeterioration and correlations between trace elemental results and diagenetic alteration. Micro-sampling of intact portions of bone expands the scope of available materials for research on mobility and other aspects of human past behavior.     

New evidence of prehistoric arrow wounds in the Iberian Peninsula

Recent excavations carried out in the Iberian Peninsula have revealed some skeletons with arrow wounds at sites dating from the neolithic to the Bronze Age. The discovery of burials in which the human remains have not been moved and the methodical nature of the excavation have made an in situ examination possible, and have confirmed this evidence as testimony of violent action, of which there are many other examples all over Europe. Four cases of silex arrowheads found in different human bones from the excavation of the hypogeum of Longar (Navarra, Spain), carried out between 1991 and 1993, are described.     

Arsenic in Danish and Swedish Mesolithic and Neolithic human bones – diet or diagenesis?

Arsenic has previously been measured in a bone sample of a c. 5-year-old Mesolithic child from Nivaagaard in Denmark yielding an anomalously high value of 13 μg/g. In the present work we show that other individuals at a nearby site also exhibit elevated arsenic levels, whereas individuals from other Mesolithic sites in Denmark are found to have quite low As-concentrations. Several attempts to reveal the source of the arsenic are reported: A railway track near the site, where the railway sleepers were suspected to have been impregnated with As leading to transport or diffusion of As into the adjacent soil; Several food items, the remains of which were found at the site, and one food item not preserved in the excavation, i.e. seaweed. Finally, it is shown that the most likely explanation of the elevated arsenic levels in human bones at Nivaa is diagenesis.     

Diameter of the human internal acoustic meatus and sex determination

We report the results of testing the diameter of the internal opening of the acoustic canal in the petrous part of the temporal bone for sex determination of skeletal remains. The method involves measuring the diameter using a suite of ordinary drills. The method is very simple and has the great advantage of utilising one of the sturdiest bone elements of the human skeleton. The method may be especially useful for the analyses of very fragmented skeletal remains or cremated bones, where the petrous bone may still be readily recognisable. The method was tested using a forensic sample of 113 left petrous bones with known sex. Intra‐ and inter‐observer testing was also performed. We found a statistically significant difference in diameter between males and females (means: males: 3.7 mm; females: 3.4 mm; P < 0.009). However, the low predictive value (70%) for correct sexing using two sectioning points ( < 3.0 mm = female; >3.5 mm = male) was disappointing. No additional accuracy was gained by employing both left and right petrous bones (a bilateral sample of 60 petrous bones was also tested), although left and right side diameter is highly correlated (R = 0.778; P = 0.0001). Copyright © 2005 John Wiley & Sons, Ltd.     

成都市新都区新石器时代晚期土坑墓提取与保护

2008年1月,成都文物考古研究所与成都市新都区文物管理所在对一新石器时代晚期聚落遗址进行的考古发掘中,清理出土了二座竖穴土坑墓,其中M1墓葬人骨基本完整,具有较高的研究价值,但因年代久远和有害环境的影响,骨质已粉化,不便于直接提取人骨。为了有效地保护这一新石器时期人类骨骸,为后期的科学研究保存下这一珍贵的实物资料。决定采取套箱法对整座土坑墓进行提取的方式保护人骨,经过多方试验,确定采用A305黏结剂修复骨架裂纹,用GSA-102保护剂,B365作加固进行保护。经观察表明,本次土坑提取与保护取得了良好效果,并制定了一套科学的保护方案。该土坑墓的成功提取和科学保护,为今后在潮湿环境中的古遗址保护提供了可供借鉴的经验。     

Skates and Prickers from the Circular Fortress of Oost-Souburg,The Netherlands (AD 900–975)

Abstract

During the excavation of the circular fortress of Oost-Souburg, the Netherlands, objects of bone and antler were found: combs, needles, spindles, three-pronged objects, bone skates and points. The finds are dated between AD 900 and 975. In this contribution the objects which belong to the winter season, the bone skates and the points, are discussed. The skates occur in various types and are made of a variety of bones. Certain types of skate were used without bindings, others were either designed to be fastened to the feet or possibly were tied only at the heels. The roughly-made points that were found may be the points of the prickers used to propel the skater forward on his skates.     

‘Rid grasse of bones’: A taphonomic study of the bones from midden deposits at the Neolithic and Bronze age site of Runnymede,Surrey, England

Runnymede has large samples of Neolithic and Late Bronze Age animal bones, with contrasting preservation conditions in both periods. The bone evidence has been used to interpret the formation of the site deposits. There are few articulated bones, and no joins were found in butchered bone, indicating that the area studied did not contain primary refuse. Various aspects of bone alteration have been analysed: (i) the proportion of bones with very good surface preservation was high in the in situ Neolithic excavation units and the basal Bronze Age midden, but bones in the upper units were mostly eroded. These units are reworked flood deposits. The greater degree of fragmentation of the bone in the reworked units has been quantified, using a system of recording the ‘zones’ present on each bone, which allows calculation of the fraction present. It is also demonstrated that the reworked units contain a lower proportion of identified bones and a higher proportion of teeth and iaws than the units with well-preserved bone, (ii) Quantification of canid gnawing shows, unexpectedly, that more was recorded on well-preserved bone. Thus recognition of gnawing depends on bone condition. This also confirms that most of the erosion of the bone surface is a post-depositional phenomenon. The sequence of activities is therefore interpreted as follows: meat was cooked and consumed, and the bones discarded for the dogs. At a later stage, larger bones were picked up and thrown away in the river or midden. Some ethnographic examples of periodic cleaning of farming settlements are cited.     

‘Old wood’ effect in radiocarbon dating of prehistoric cremated bones?

Numerous reports of successful radiocarbon dating of cremated bones have emerged during the last decade. The success of radiocarbon dating cremated bones depends on the temperature during burning and the degree of recrystallisation of the inorganic bone matrix. During cremation bones undergo major morphological and mineralogical changes which have raised some interesting questions and discussion on the origin of the carbon source in archaeologically cremated bones. Recent laboratory experiments reveal that the properties of the combustion atmosphere play a significant role regarding the source carbon in cremated bones. Thus radiocarbon dating cremated bones is potentially dating the wood used for the cremation fire. Here we compare a high precision radiocarbon dated human bone with an associated dendrochronological age from an oak coffin. We find that the age discrepancy between the dendrochronological age and the cremated bone of 73 ± 26 ¹⁴C yr is best accounted for by the so called ‘old wood’ effect.     

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones.     

Pick the Right Pocket. Sub‐sampling of Bone Sections to Investigate Diagenesis and DNA Preservation

Many archaeological bones display a heterogeneous degradation pattern. Highly degraded bones could contain pockets of well‐preserved bone, harbouring good quality DNA. This dichotomy may explain why the relationships between global bone preservation parameters such as histological integrity, bone mineral crystallinity or collagen yield, and bulk DNA preservation/amplification success rate have been found to be at best, weak to moderate. In this pilot study, we explore whether or not a more localised approach will highlight a stronger relationship between diagenetic parameters and DNA preservation. This study includes a detailed histological characterisation of bone diagenesis in sub‐areas of three bone samples. Regions of the same bone, which displayed differential degrees of preservation or type of diagenesis were sampled for further analysis and both genetic (small scale Illumina MiSeq sequencing) and chemical (Fourier‐transform infrared spectrometric analysis) analyses were performed. The aim was to investigate how bone diagenetic processes relate to DNA preservation at a higher resolution than in previous studies. This is key in order to improve DNA analytical success rates. The expected relationship between bone and DNA preservation (retrieved endogenous DNA) was observed and the results corroborate previous work that DNA preservation is linked to the integrity of bone collagen and mineral. The results further suggest that non‐biological diagenetic alterations such as etching and the presence of mineral infiltrations and inclusions have a negative effect on DNA preservation/extraction. Copyright © 2016 John Wiley & Sons, Ltd.     

Butchering and cooking of birds in the palaeolithic site of Grotta Romanelli (Italy)

Grotta Romanelli is one of the most important sites of the Italian palaeolithic. It contains a lithic industry from the Final Epigravettian, examples of rock and mobiliary art and numerous bone remains, among which were abundant remains of birds. Approximately 32 000 bird bones from over 3650 individuals and 109 species were identified. The most common species were bustards, Otis tetrax and O. tarda, and three species of goose, Anser fabalis, A. albifrons and Branta bernicla. Traces of butchering and burning were evident on numerous bones. The nature and location of the cut marks and burning is described here. The analysis has involved mostly the hind limbs and the shoulder girdle where traces are most numerous. The cut marks reflect a codified sequence of actions of disarticulation and dismemberment. © 1997 John Wiley & Sons, Ltd.     

Site conservation during excavation: treatment of masonry,wall plaster and floor mosaic remains of a Byzantine church in Petra,Jordan

Abstract

The conservation treatments carried out between June 1992 and August 1993 on the architectural remains of a Byzantine basilica in Petra, Jordan, during and immediately after the excavation are described. The treatments completed during excavation included: stabilization and consolidation of certain sections of the basilica's sandstone ashlar masonry; cleaning and consolidation of the unpainted lime wall plaster decorating the basilica interior; cleaning and consolidation in situ of the extensive remains of a figurative mosaic pavement in the two side aisles, and the fragmentary remains of the opus sectile pavement in the central aisle. The consolidation treatments included injection grouting using hydraulic mortar mixes produced in Italy, and mortar repairs using slaked lime and hydraulic lime. The existence of the mosaic required preventive conservation measures during the excavation, such as the construction of a temporary shelter over the site during the winter, the use of temporary protective facings over damaged areas of the mosaic, as well as the temporary backfilling of the mosaic with soil over a layer of geotextile in order to protect the mosaic between the initial phase of conservation work and the construction of a permanent shelter for the site in the near future. The importance of having a conservator present on site during the critical phase of excavation, when all materials are most susceptible to damage, is emphasized. The Petra Church Project will hopefully serve as a model for future excavations in that it employed two conservators from the beginning of the excavation: one for small finds, the other responsible for the site and the architectural remains removed from the site during the excavation.     

汉晋时期河西走廊张掖地区的开发研究——以黑水国汉代墓葬为例

黑水国汉代墓葬出土的农具、动物骨骼、农作物遗存和人骨为人们了解河西走廊张掖地区汉晋时期生业经济和农业发展等问题提供了重要证据。通过动物骨骼、农作物遗存鉴定结果和人骨碳氮同位素检测结果,可以揭示汉晋时期黑水国人群的食物结构和生业模式特点。墓葬出土的农作物有粟、黍、稻,随葬动物有鸡、猪、绵羊、黄牛、狗、马,人骨骨胶原碳稳定同位素主要呈现C₃和C₄混合信号,部分呈现C₃信号和C₄信号,氮稳定同位素值相较牛羊等家养食草动物较高。综合研究说明汉晋时期河西走廊形成麦类、粟黍和豆类的混合农业模式,所以黑水国汉代先民摄取的粮食作物来源呈多样化。同时,河西走廊畜牧业发达,使当时人群摄取了大量的肉食资源,导致蛋白质摄入量较高。     

Taphonomic analysis of remains of mammals eaten by pumas (Puma concolor Carnivora, Felidae) in central Argentina

Prey remains recovered from puma (Puma concolor) scat were analyzed in order to identify taphonomic features produced on them by this predator. Mammal remains are abundant in puma scat, but recognition of bone remains decreases as prey size increases. Modifications produced by digestion are strong. However, micromammal bones show a gradation in preservation that includes well preserved specimens. Data presented here and their interpretation may possibly be extrapolated to zooarchaeological or paleontological assemblage.