A protocol for recording linear enamel hypoplasia on archaeological pig teeth

A method is proposed for recording linear enamel hypoplasia (LEH) present on archaeological pig teeth. The methods described have been developed and tested on material from five archaeological sites of a wide range of periods; Durrington Walls (UK), Wellin, Ename, Sugny and Londerzeel (Belgium). Recommendations are made on what teeth, surface, cusp, and side to record, as well as on details of the visibility, the identification and the measurement of LEH lines. Problems encountered with the application of the recording protocol to the five case studies are detailed, and possible interpretative drawbacks are discussed. © 1998 John Wiley & Sons, Ltd.     

Protocol for Recording Enamel Hypoplasia in Modern and Archaeological Caprine Populations

This paper outlines the first methodology for recording dental enamel hypoplasia in the high‐crowned dentition of modern and archaeological caprine teeth. The method has been developed and trialed on five caprine populations from Orkney (UK); two modern populations (Shetland and North Ronaldsay breeds) and three Neolithic assemblages from the archaeological sites of Knap of Howar, Skara Brae and Holm of Papa Westray. Problems associated with differential tooth wear, as well as the presence of coronal cementum, are discussed, and recommendations are given on the identification and recording of hypoplastic dental defects in caprines. Copyright © 2012 John Wiley & Sons, Ltd.     

Dental wash: a problematic method for extracting microfossils from teeth

A variety of microfossils, originating from plant foods, become trapped in the dental calculus matrix. Processing of dental calculus allows extraction of these microfossils. The resulting data can be used to reconstruct diet at the individual and population levels as the identification of microfossils like starch grains and phytoliths to the generic level, and sometimes to the species level, is possible. However, in some archaeological sites, dental calculus deposits do not preserve well enough to be processed. To prevent the loss of information in such cases, we present a technique, called “dental wash”. It permits extracting microfossils from cryptic dental calculus deposits. In the two experimental archaeological cases presented herein we identified phytoliths, starch grains as well as a diatom fragment with this method, whereas in a control sample no microfossils were found. Moderate damage to the teeth was detected when they were already friable due to poor preservation. Minor damage to the surface of well-preserved teeth was observed. This indicates that the proposed method is efficient in recovering microfossils, but unacceptable because of damage to teeth. If the method can be refined, it will expand the potential of dental calculus analysis to a greater range of archeological sites.     

Incremental banding in dental cementum: Methods of preparation for teeth from archaeological sites and for modern comparative specimens

Analysis of incremental banding in dental cementum is a well-established means of determining the age and season of death of wild mammals. The dental cementum of domesticated mammals likewise can indicate age and season of death. Methods of preparation applicable to archaeological teeth differ from those used for modern specimens, however, and this paper describes two methods that have given excellent imaging on teeth of Bos taurus; one for modern teeth and the other for teeth from archaeological sites.     

Defining an indicator package to allow identification of ‘cesspits’ in the archaeological record

This paper summarises the insect, plant macrofossil and other environmental evidence from a large number of deposits, thought to be cesspits, at a range of archaeological sites. A potential ‘indicator package’ (sensu Kenward and Hall, 1997), consisting of a range of biological materials and archaeological artefacts, is outlined which should allow a more accurate identification of cesspits in the archaeological record enhancing further studies of the rich evidence often preserved in them.     

Information obtained from the microscopic examination of cultural striations in human dentition

The surfaces of teeth from some fossil remains and archaeological samples have been examined by SEM, in order to study different types of cultural striations and to determine the archaeological information that these striations can provide. Different patterns of cultural striations are present in human samples from different sites and periods. Similar cultural striations can be interpreted in different ways, depending on the tooth in which they are present, their location in the tooth and their direction, regularity, number and width. The study of cultural striations can yield valuable information about different aspects of feeding behaviour, oral hygiene, patterns of cultural diffusion and manipulative work with teeth of past human groups. A close integration with other anthropological and archaeological data is needed and specific interpretations should be made in each case.     

X‐RAY SUB‐MICRON TOMOGRAPHY AS A TOOL FOR THE STUDY OF ARCHAEOLOGICAL WOOD PRESERVED THROUGH THE CORROSION OF METAL OBJECTS

Wood preserved in the corrosion layer of two early medieval iron objects was examined using X‐ray tomography. A state‐of‐the art multi‐resolution X‐ray tomography set‐up ( http://www.ugct.ugent.be ) provided virtual cross‐sections of the archaeological wood samples at sub‐micron resolution. These were compared with scans of samples of similar modern wood. These scans demonstrate the power of sub‐micron X‐ray tomography for wood identification, although the process of mineralization pushes this technique to its limits. Furthermore, this technique facilitated appraisal of the mineral content of the archaeological wood, which is useful in selecting the most appropriate strategy for the (preventative) conservation of the archaeological object.     

Morphological distinctions between the mandibular teeth of young sheep, Ovis, and goats, Capra

Morphological distinctions between the mandibular teeth of young sheep (Ovis) and goats (Capra) are described, and their value in the study of bone samples from archaeological sites is briefly discussed.     

Morphometric analysis of limpets from an Iron-Age shell midden found in northwest Portugal

The present work reports an analysis of a shell midden found in Terroso hillfort, an important Iron-Age oppidum located in northwest Portugal. The midden was dated from the latest phase of occupation of the hillfort, between the Ist century BC and the Ist century AD (Roman period), and contained 684 well-preserved Patella shells. The identification of archaeological shells was carried out comparing them with modern specimens collected in two shores in the neighborhood of the hillfort. The identification of modern shells was based on radula pluricuspid teeth and shell morphology. Relative abundance of Patella species in the Terroso midden was different from modern populations. Archaeological shells were dominated by Patella vulgata, but Patella intermedia was the most abundant species in modern populations. Dimensions of archaeological shells were very different from modern populations. Shell length range and variability in archaeological shells were lower than in the modern populations. Log height vs. length plots for archaeological shells were different from modern populations. Patella shells from Terroso midden was significantly taller then modern specimens. These differences between archaeological shells and modern populations could be due to environmental changes, namely an increase in wave action and intensity. These harsher costal conditions could have resulted from an alteration in the morphology of the coast, caused by a rise in the mean sea level from 2000 years BP to the present. Alternatively or additionally, the observed differences can be related to gathering strategies. Archaeological shells could have been selected by size, and collected preferentially at high shore and in sheltered sites.     

Identification of wood from archaeological sites in the American Southwest. I. Keys for gymnosperms

Keys for the identification of gymnosperm wood remains from archaeological sites in the American Southwest are presented. Wood identification and analysis are briefly discussed.     

Recording dental caries in archaeological human remains

Dental caries is an important condition to record in archaeological collections, but the way in which recording is carried out has a large effect on the way in which the results can be interpreted. In living populations, dental caries is a disease that shows a strong relationship with age. Both the nature of carious lesions and their frequency change with successive age groups from childhood to elderly adulthood. There is also a progression in the particular teeth in the dentition which are most commonly affected and, in general, the molars and premolars are involved much more frequently than the canines and incisors. Lower teeth are usually affected more than upper, although the condition usually involves the right and left sides fairly equally. In the high tooth wear rate populations represented by many archaeological and museum collections, there is a complex relationship between the form of lesions and the state of wear, which adds yet another range of factors to the changing pattern of caries with increasing age. In the same populations, chipping, fracture and anomalous abrasion of teeth are also common, and these contribute similarly to the distribution and forms of carious lesion observed. Amongst the living, the pattern of ante‐mortem tooth loss is important in understanding caries and, in archaeological material, there is also the complicating factor of post‐mortem tooth loss. Finally, there is the question of diagnosis. There are diagnostic problems even in epidemiological studies of living patients and, for archaeological specimens, diagenetic change and the variable preservation of different parts of the dentition add further complications. For all these reasons, it is difficult to define any one general index of dental caries to represent the complete dentition of each individual, which would be universally suitable for studying a full range of collections from archaeological sites or museums. Variation in the nature of collections, their preservation, tooth wear, and ante‐mortem and post‐mortem tooth loss mean that when such a general index appears to differ between sites, there could be many other reasons for this, in addition to any genuine differences in caries incidence and pattern that might have been present. It is suggested here that the best approach is instead to make comparisons separately for each tooth type, age group, sex, lesion type and potential lesion site on the tooth. Copyright © 2001 John Wiley & Sons, Ltd.     

NON‐DESTRUCTIVE ANALYTIC METHOD USING XRF FOR DETERMINATION OF PROVENANCE OF ARCHAEOLOGICAL OBSIDIANS FROM THE MEDITERRANEAN AREA: A COMPARISON WITH TRADITIONAL XRF METHODS*

A non‐destructive analytical method using wavelength‐dispersive X‐ray fluorescence (WDXRF) that allows the establishment of the provenance of archaeological obsidians was developed and a comparison with the classical XRF method on powders is discussed. Representative obsidian samples of all the geological outcrops of archaeological interest of the Mediterranean area (Lipari, Pantelleria, Sardinia, Palmarola and the Greek islands of Melos and Gyali), were analysed with the normal procedures used in rock analysis by XRF (crushing, powdering and pelletizing). The non‐destructive XRF analysis was instead conducted on splinters taken from the original geological pieces, with the shape deliberately worked to be similar to the refuse usually found at archaeological sites. Since the analysis was conducted on the raw geological fragment, intensity ratios of the suitably selected chemical elements were used, instead of their absolute concentrations, to avoid surface effects due to the irregular shape. The comparison between concentration ratios (obtained by traditional XRF methods) and the intensity ratios of the selected trace elements (obtained from the non‐destructive methodology) show that the different domains of the chemical composition, corresponding to the geological obsidians of the source areas, are perfectly equivalent. In the same way, together with the geological splinters, complete archaeological obsidians, from Neolithic sites, may be analysed and their provenance may be determined. The proposed non‐destructive method uses the XRF method. Due to its sensitivity, low cost and high speed, it is surely an extremely valid instrument for the attribution of the provenance of the archaeological obsidian from Neolithic sites.     

Avian egg-shell from archaeological sites

Avian egg-shell occurs fairly commonly on archaeological sites, particularly in alkaline deposits. It is rarely studied in detail. Egg-shell structure is described briefly below and features which may be of value for identification purposes are discussed. Egg-shells from 14 archaeological sites are tentatively identified as goose, domestic fowl, duck and guinea fowl. It seems likely that confident identification should ultimately be possible using a combination of features. This should yield information complementary to osteological evidence for the history of the utilization of birds and their eggs.     

Identifying archaeological wood stack charcoal production sites using geophysical prospection: magnetic characteristics from a modern wood stack charcoal burn site

Charcoal making was a common process in the woodlands of Britain for many centuries. However, historic ‘wood stack’ production sites are difficult to identify. This paper tests the hypothesis that geophysical survey is an appropriate method for the discovery and identification of archaeological charcoal making sites. A traditional wood stack charcoal kiln was constructed in Low Staindale, Dalby Forest, near Pickering, North Yorkshire, to investigate the charcoaling process and the effect of low-temperature carbonisation on the magnetic properties of the soil underlying the kiln. The results from temperature monitoring (within and beneath the charcoal stack) throughout the charcoaling process, geophysical surveys across the charcoal kiln platform, and laboratory analysis of soil and ash samples, are presented. The degree to which the magnetic properties of the ground beneath the charcoal kiln had been enhanced as a consequence of this low-temperature process is discussed. Whilst some magnetic enhancement to the soil beneath the kiln platform was recorded, enhancement appears to be the result of contamination from the wood stack sealing material rather than the effect of heat transfer resulting from the charcoaling process. The results suggest that whilst historic wood stack charcoal production sites are likely to remain an enigmatic and under-reported feature in the archaeological landscape, geophysical prospection does have the potential for identification of these sites.     

ZooMS: making eggshell visible in the archaeological record

Avian eggshell is a common component of many archaeological deposits, but its archaeological potential remains largely unexplored. The most obvious reasons are two-fold. Firstly, despite its abundance on many sites, eggshell is often overlooked during excavation. Even when it is recovered, small fragmented remains are difficult to identify taxonomically. Here we introduce a minimally destructive qualitative analytical technique for taxonomic identification of eggshell fragments based on highly sensitive mass spectrometry and peptide mass fingerprinting (ZooMS), and illustrate its application to eggshell recovered from the Viking Age urban site at Hungate, York. We adopt a more extreme version of the method of bleach treating used to prepare ancient eggshell for DNA analysis, followed by conventional peptide mass fingerprinting using MALDI-ToF mass spectrometry. The development of this technique will allow future research to make better use of eggshell fragments recovered from archaeological sites.     

The molecular palaeoecology of geese: identification of archaeological goose remains using ancient DNA analysis

The remains of six species of geese are commonly recovered from archaeological sites in Britain dating from the Saxon and later periods. However, identification of this material to species level is hampered by a lack of morphological variation and a large overlap in size. To address this issue we obtained DNA sequence data for a section of the mitochondrial cytochrome b gene from modern samples of each species, and successfully identified several DNA markers for Branta species. No markers were found within the cytochrome b gene for the genus Anser. Ancient DNA techniques were then used to recover DNA from goose bones excavated from two archaeological sites. The DNA sequences enabled identification of Barnacle goose (Branta leucopsis) from one site and confirmed the presence of Anser species at another. © 1998 John Wiley & Sons, Ltd.     

Sex estimation using cervical dental measurements in an archaeological population from Iran

Sex estimation of skeletal remains is one of the major components of forensic identification of unknown individuals. Teeth are a potential source of information on sex and are often recovered in archaeological or forensic contexts due to their post-mortem longevity. Currently, there is limited data on dental sexual dimorphism of archaeological populations from Iran. This paper represents the first study to provide a dental sex estimation method for Iron Age populations. The current study was conducted on the skeletal remains of 143 adults from two Iron Age populations in close temporal and geographic proximity in the Solduz Valley (West Azerbaijan Province of Iran). Mesiodistal and buccolingual cervical measurements of 1334 maxillary and mandibular teeth were used to investigate the degree of sexual dimorphism in permanent dentition and to assess their applicability in sex estimation. Data was analysed using discriminant function analysis (SPSS 23), and posterior probabilities were calculated for all produced formulae. The results showed that incisors and canines were the most sexually dimorphic teeth, providing percentages of correct sex classification between 86.4 and 100 % depending on the measurement used. The combination of canines and other teeth improved significantly the level of correct sex classification. The highest percentages of sex classification were obtained by the combination of canines and incisors (100 %) and canines and molars (92.3 %). The present study provided the first reference standards for sex estimation using odontometric data in an Iranian archaeological population. Cervical measurements were found to be of value for sex assessment, and the method presented here can be a useful tool for establishing accurate demographic data from skeletal remains of the Iron Age from Iran.     

An Investigation of Possible Fluorosis in Human Dentition Using Synchrotron Radiation

Macroscopic analysis of collections of archaeological human dentition from sites in the United Arab Emirates varying in chronological age and geographical location revealed the presence of enamel defects on the buccal, lingual and occlusal surfaces of some of the teeth. The morphology of these enamel defects suggested a possible diagnoses of fluorosis. Because not all teeth in the samples studied exhibited the defect, a conclusive diagnosis could not, however, be offered. Given that fluoride can enter the crystal lattice of hydroxyapatite (the major mineral component of calcified tissue including bones and teeth), and that the uptake of higher than optimal levels of fluoride can alter the molecular structure of dentition, it was thought that the application of X-ray diffraction to this problem may aid a more conclusive diagnoses. This paper details the application of synchrotron radiation to this problem. Initially, details of the archaeological problem are outlined. This is followed by a discussion of synchrotron radiation and the advantages it has over conventional X-rays. The materials and methods used in the analysis are provided, followed by a discussion of the results. While further work is required to fully attribute the enamel defects to the effects of fluoride, this paper illustrates that synchrotron radiation, in particular X-ray diffraction, can be applied to archaeological materials as a means of quickly and effectively obtaining a structural analysis.     

The use of dental criteria for estimating postnatal survival in skeletal remains of infants

Separate cemeteries and/or burial loci for infants have been variously interpreted as reflecting segregation by age, infanticide or even child sacrifice. Attempts to distinguish between these factors rely primarily on the age distribution found. Currently long bone length is the most commonly used method for fetuses and infants in the perinatal period, but its accuracy is affected by the inherent variation in size for age. We show here how to distinguish between death in the perinatal period and that occurring later in infancy through identification of the neonatal line in ground sections of deciduous teeth. The methodology is reviewed and applied to validate estimations of postnatal survival for infant remains recovered from two archaeological sites in Israel.     

Immigration to Tikal,Guatemala: Evidence from stable strontium and oxygen isotopes

This paper presents strontium and oxygen isotopic measurements on archaeological human teeth from the ancient Maya city of Tikal, Guatemala, that illuminate the role that migration played in the history of the state. Stable strontium isotope ratios of human teeth parallel the bedrock geology of the location where foods were grown, while stable oxygen isotope ratios reflect the sources of water imbibed, and track geographic variation in the isotopic composition of rain water. Because tooth enamel forms during childhood and is not remodeled during life, we can identify foreign-born individuals at Tikal by their outlying strontium and oxygen isotope ratios. These data indicate that approximately 11–16% of the sampled Tikal skeletons spent their childhood at distant sites. Most of the migrant burials date from the Early Classic period and are high status contexts. Several royal burials demonstrate long distance movement of both males and females, and shed light on the identification of epigraphically-known individuals. Yet, both Early and Late Classic migrants are found in lower status domestic burials. Interaction with distant peers was important in the rise of the Tikal polity, however, immigration from all social tiers contributed to the city’s rapid population growth.