Ultrastructure and DNA sequence analysis of single Concentricystis cells from Alta Val Tiberina Holocene sediment

We successfully combined ultrastructure and chloroplast DNA analysis for single specimens of Concentricystis isolated from Holocene sediment by a palynology method based on filtration, progressive sieving and percoll-gradient sedimentation to concentrate fossil cells. We compared our method with the traditional harsh chemical treatment commonly used to isolate fossil pollen and spores. With our method, the cytoplasm of Concentricystis was sufficiently preserved to distinguish several cell structures by light and electron microscopy. DNA analysis of Concentricystis involved PCR amplification using specific primers for a spacer region of the chloroplast genome. Significant homologies were found with the Angiosperm chloroplast genome by BLAST DNA search for PCR product sequences.     

Ancient DNA,a Neolithic legging from the Swiss Alps and the early history of goat

Ancient DNA from a Neolithic legging (1st half of the 3rd millennium BC) found at Lenk, Schnidejoch (2750 m a.sl.) in the Swiss Alps has demonstrated, that modern distribution of genetic variation does not reflect past spatio-temporal signatures. The legging was made from the skin of a domestic goat (Capra hircus), belonging to the caprine haplogroup B1, which is marginal in Europe today, but represents a third highly diverse goat haplogroup entering Europe already in the Neolithic. Population expansion of lineage B therefore happened more than 4500 years ago, but their members were at some point almost completely replaced by goats of today's common A and C haplogroups.     

Variability in the lithic and faunal record through 10 reoccupations of a XIX century Yamana Hut

The excavation of Tunel VII, a Yamana site dating to the indigenous/European contact period was part of a long term research project based on the north coast of the Beagle channel (Tierra del Fuego, Argentina). The aim was to evaluate the theory and methodologies and devise an archaeological method that would enable a complete picture of subsistence strategies to be constructed. At Tunel VII (a site with shell middens), we were able to analyse these strategies through 10 successive occupation events on a single location. Archaeozoological analysis of the faunal remains and use-wear analysis of lithic material were used to examine the management of resources. Production and consumption are two very useful concepts, and together they have been used to create a methodology, which, together with spatial analysis using significant variables, has enabled identification of recurrent or significant tendencies in relation to alteration or continuity in subsistence strategies. In the case of Tunel VII, we know that the people who continually occupied the hut were all from the same group.     

Stable carbon- and nitrogen-isotope ratios and electron spin resonance (ESR) g-values of charred bones: changes with heating and a critical evaluation of the utility of g-values for reconstructing thermal history and original isotope ratios

Changes in the stable carbon and nitrogen isotope ratios of modern bone samples heated to a variety of times and temperatures were used to determine the effect of heating on isotope ratios and the retention of organic matter in charred bones. For organic extracts produced by slow demineralization in weak acid, δ¹³C values were unchanged, while δ¹⁵N values increased by up to 5‰ and were primarily determined by heating temperature. Changes in the electron spin resonance (ESR) g-value of whole bone and organic extracts were also measured. For organic extracts from charred bones, the g-value was well-correlated with δ¹⁵N and temperature, suggesting that g-values could be used to estimate the charring temperature and original δ¹⁵N values of charred bones. Thus, g-values from demineralized extracts could be very useful in forensic investigations where it would important to reconstruct the thermal history of burned bones. Isotope ratios and g-values of demineralized extracts from four prehistoric components at three sites that produced cremated human bone were used to test whether the same approach can be applied to archaeological materials. While carbon isotope ratios of the prehistoric samples were similar to those of uncharred specimens, nitrogen isotope ratios were increased and the g-value corrections for nitrogen isotope ratios were not effective.     

King Stephen,the English church,and a female mystic: Christina of Markyate's Vita as a neglected source for the council of Winchester (August 1139) and its aftermath

One of the central reasons for the disintegration of royal authority (sometimes called ‘the Anarchy’) during the reign of King Stephen of England is generally thought to have been his troubled relationship with the English church. The king was summoned to appear before the legate in England, Henry of Blois, bishop of Winchester (who was also Stephen's brother), at a church council called for Winchester on 29 August 1139, in order to show cause for his conduct in arresting several prominent bishops and in confiscating their property. Several major chroniclers discuss the events leading up to and occurring at the council of Winchester, especially William of Malmesbury in his Historia novella and the anonymous Gesta Stephani. The versions of events contained in these sources are not entirely consistent. The present paper examines yet another recounting of the events of the council, seldom appreciated by historians of twelfth-century England, presented in the Vita of Christina of Markyate (c.1096/98–c.1155/66), composed by an anonymous monk of St Albans between 1140 and 1146. Christina was close to the abbot of St Albans, Geoffrey de Gorham, who was probably the patron of the Vita and who quite likely attended the Winchester council and apparently became involved in its aftermath. These events are recorded in some detail in the Vita, presenting us with a vivid recounting of the council and the immediate consequences thereof. The narrative of the Vita contains a somewhat different picture of the personalities and occurrences surrounding the Winchester council than we encounter in the chronicles. The current essay compares the Vita to the standard accounts. We argue that the Vita may be the earliest and possibly most reliable source for the events of the council. Moreover, if we privilege the report of the Vita, the council becomes an especially significant moment in the breakdown of relations between Stephen and the English church.