A Pilot Study in Odontochronology for the Pig Domestication Question in North‐Central China

In this paper, we report results of the pilot study in which we apply skeletochronological analysis of teeth to a collection of modern Chinese wild boar (Sus scrofa) of known age and date of death. This is the first time that odontochronology has been applied to suids in China. This work allowed us to establish parameters for subsequent analysis of a limited number of Neolithic‐aged Sus (wild boar and possible early domesticated pig) samples from Henan Province. The goal of the research is to contribute to the ongoing reconstruction of Neolithic dietary, health and economic patterns along the Yellow River. Our results are encouraging in that regular seasonal growth patterning is observed in the northern control sample, which is from the same region as our archaeological investigation. Copyright © 2011 John Wiley & Sons, Ltd.     

Ancient olive DNA in pits: preservation,amplification and sequence analysis

The olive tree (Olea europaea) was domesticated by vegetative propagation of selected wild individuals with superior fruit. Later, new cultivars were established repeatedly from feral trees or from crosses between wild, feral, and domesticated trees. Thus the genetic background of many contemporary domesticated lines is a mixture of ancient cultivars and local wild trees. Ancient DNA may illuminate the complicated process of olive domestication because such DNA sequences provide data about ancient genomes that existed closer to the domestication events. Well preserved DNA must be available for such studies, even though in the Mediterranean region, where olive cultivation took place, the climatic conditions are not favorable for DNA preservation. To select for well preserved pits we measured their proportions of lignin by IR spectroscopy, and correlated this with parameters of DNA quality such as template length in an olive-specific repeat array, and template quantity as determined by real-time PCR amplification. Archaeological pits that passed these tests did contain high quality ancient DNA. We present the first ancient olive DNA sequences and compare them to modern wild, feral and domesticated lines.     

Early Neolithic diets at Baijia,Wei River valley,China: stable carbon and nitrogen isotope analysis of human and faunal remains

Stable carbon and nitrogen isotope values are presented for faunal and human bone collagen from Baijia, in the Wei River valley region of Shaanxi Province, China. The remains have a calibrated age range of ca. 5709–5389 BC, and correspond with the early Neolithic Laoguantai Period. Stable isotopic results indicate that human diets included millet and probably aquatic foods such as fish and shellfish. Bovid samples are tentatively identified as water buffalo, and have a mean δ¹³C value of −14.6‰, which reflects some millet consumption. Whether bovids were grazing on wild millet, or had diets directly influenced by humans, is not known. The single Sus sample from Baijia had a diet dominated by C3 plants and is thus unlikely to have been a domesticated animal. Overall, the stable isotope results presented here conform to the current concept that the people of the Laoguantai culture were millet farmers, who had subsistence strategies that included hunted wild foods.     

Early Pig Management in the Zagros Flanks: Reanalysis of the Fauna from Neolithic Jarmo,Northern Iraq

In this paper, we present a reanalysis of pig (Sus scrofa) remains from the Neolithic site of Qalat Jarmo, originally excavated in the 1940s and 1950s. Employing modern zooarchaeological techniques, not available during the initial analyses, we explore the nature of swine exploitation strategies and demonstrate that pigs were most likely managed by the early 7th millennium (Pottery Neolithic) and perhaps earlier. Comparing biometric data with those from other sites in the region, we show that the Jarmo pigs exhibit evidence for size decrease associated with intensive management, but had not yet achieved the degree of dental or post‐cranial size reduction seen in later Neolithic domestic populations. Although samples from the earliest (Pre‐Pottery) occupation of the site are small, there is some evidence to suggest that domestic pigs were present at Jarmo as early as the late 8th millennium cal. bc . In either case, Jarmo likely represents the earliest appearance of pig husbandry along the Zagros flanks, and we discuss the mechanisms by which Neolithic technologies, including domesticated animals, spread to new regions. This project emphasises the value of curated faunal assemblages in shedding new light on the spread of Neolithic economies. Copyright © 2013 John Wiley & Sons, Ltd.     

Mitochondrial DNA Analysis of Ancient Domestic Goats in the Southern Caucasus: A Preliminary Result from Neolithic Settlements at Göytepe and Hacı Elamxanlı Tepe

This study presents preliminary results of mitochondrial DNA analyses of modern and ancient domestic goats in the southern Caucasus in order to examine their phylogenetic relationship with modern and ancient goats. For this purpose, seven ancient samples were obtained from two early agricultural villages in west Azerbaijan (Göytepe and Hacı Elamxanlı tepe, dated to ca. 6000–5500 cal bc , the Pottery Neolithic period), in addition to five modern goat samples in the same region. In the study, mitochondrial DNA segments of the control region (216 bp for the Neolithic samples and 481 bp for the modern samples) were amplified, and phylogenetic analyses were performed using previously published reference DNA sequences. As a result, all the haplotypes found in this study were grouped in the haplogroup A of goats. The finding of the haplogroup A among domestic goats in the southern Caucasus in the early sixth millennium bc can be interpreted as part of the geographic expansion of this lineage from the areas of initial domestication to surrounding areas that include also South and Southeast Europe. In the southern Caucasus, the haplogroup A probably continued to be a major lineage among domestic goats since their emergence in this area to the present. In contrast, this lineage has not been detected among local wild goats including Capra aegagrus , indicating the external origin of domestic goats. This possibility is consistent with archaeological records that indicate sudden appearance of agricultural lifeways in the southern Caucasus and cultural connections with northern Mesopotamia. Copyright © 2016 John Wiley & Sons, Ltd.     

DNA analysis of archaeological sheep remains from China

Recent research has thrown considerable light on the history of the domestic sheep, but has not extended to ancient sheep specimens. In the present study, ancient DNA analysis was carried out on eight archaeological sheep remains recovered from Erlitou archaeological site in Henan Province (ca. 2100–1800 B.C.) to explore the genetic structure of ancient sheep and the phylogenetic relationship between ancient and modern sheep. We analyzed the control region sequences and coding regions of mitochondrial DNA from the remains by direct sequencing and restriction fragment length polymorphism analysis, respectively. Our results reveal that all ancient sheep belong to lineage A defined by modern sheep sequences. Phylogenetic analysis shows that neither argali (Ovis ammon) nor urial (Ovis vignei) mtDNA is closely related to Erlitou ancient sheep. In addition, our results suggest that ancient DNA analysis can serve as a powerful tool in tracing prehistoric population movement.     

Ancient DNA supports lineage replacement in European dog gene pool: insight into Neolithic southeast France

We report palaeogenetic analysis of domesticated dog (Canis familiaris) remains excavated from three archaeological sites from southeast France and dating from Middle Neolithic. Ancient DNA analysis was attempted on teeth and bone samples taken from 11 dogs. Three 266-base-pair fragments of the mitochondrial genome Hypervariable Region I (HVR-I) could be retrieved and revealed two haplotypes belonging to HVR-I lineage C. These three sequences were compared to the sequences of Swedish and Italian Neolithic dogs and permitted to confirm that clade C was largely represented all over Western Europe during this period. One haplotype defined in Neolithic French dog was observed for the first time in Canis mtDNA, underlining the loss of mitochondrial diversity in Europe since the Neolithic. Finally, these results point out mitochondrial lineage replacement in Europe, since lineage C represents only 5% of extant European dogs. Altogether, these results support the proposition that palaeogenetic studies are essential for the reconstruction of the past demographic history and the domestication process of dogs.     

The first discovery of Neolithic rice remains in eastern Taiwan: phytolith evidence from the Chaolaiqiao site

Located in the key junction between mainland China and Island Southeast Asia, Taiwan is of great significance for our understanding of the southeastward dispersal of rice agriculture in the prehistoric period. Until now, quite limited archaeobotanical work has been done in this region. In eastern Taiwan, no archaeological evidence of rice agriculture has been obtained, probably owing to the poor preservation conditions for plant macroremains. Here, we report a new discovery of 4200-year-old domesticated rice remains at the Chaolaiqiao site, which for the first time in detail demonstrates the ancient practice of rice agriculture in this area. Based on a combination of factors that include a rice-based plant subsistence strategy, the mid-Holocene limits to available farmland and the fast-growing Taiwan Neolithic population from settlement pattern data, we infer that this contradiction in eastern Taiwan between land-dependent agriculture and limited suitable farmland encouraged a population movement out of Taiwan during the Middle Neolithic period.     

Hesitant hunters: a review of the introduction of agriculture in western Norway

Abstract

Evidence for the introduction of agriculture in western Norway is presented, using three categories of data: (1) palaeobotanical data, including pollen diagrams from lakes, bogs and archaeological sites, focusing on the presence of cereals, Plantago lanceolata L. and anthropogenic pollen indicators, and charred macro remains of cereals from archaeological sites; (2) osteological data, focusing on the occurrences of bones of cattle, sheep and goats in three rock-shelters, and the bone material from one open-air Neolithic site; (3) archaeological data, including artefacts indicating agricultural practices, distribution of residential settlement sites, and stray finds. The evidence for agricultural activity at the beginning of the fourth millennium BC (Early Neolithic, EN) is low, whereas the presence of both cereals and animal husbandry is indicated in the palaeobotanical material from the Middle Neolithic A (MNA, 3400–2600 cal. BC). The earliest record of domesticated animal bones is dated to the Middle Neolithic B (MNB, 2600–2200 cal. BC), while palynological and archaeological data also indicate an expansion in the area cultivated by early farmers. All data confirm the establishment of an agrarian society and animal husbandry in the Late Neolithic (LN, 2200–1700 cal. BC). It is concluded that agriculture was introduced into western Norway by the indigenous hunter-fisher populations. During this process, social and ideological factors played principal roles.     

Ancient DNA Analysis of Anatolian Goat Remains Excavated from a Urartian Castle in Eastern Turkey

In recent years, mitochondrial diversity of goats has been extensively studied, in order to shed light on domestication processes. There are limited studies on genetic diversity and demographic history of Anatolian goat breeds, and these studies have focused only on modern goats. Until today, no research has been conducted on DNA analysis of ancient Anatolian goats. In the present study, seven ancient DNA samples of goat remains from Van‐Yoncatepe archaeological site in Eastern Anatolia, Turkey, dating 1000 BC, were successfully extracted and amplified for the first time. A 230‐bp fragment of mitochondrial DNA Hypervariable Region 1 of the samples was analyzed. All of the ancient sequences belonged to haplogroup A, which is the most widely distributed and frequently encountered haplogroup in modern goats. The results also pointed out that Anatolian ancient goats might have contributed to the genetic structure of modern goats in the Near East, the Mediterranean Region and South‐Southeastern Asia. Copyright © 2014 John Wiley & Sons, Ltd.     

The origins of agriculture in North-West Africa: macro-botanical remains from Epipalaeolithic and Early Neolithic levels of Ifri Oudadane (Morocco)

This research aims to shed light on the early stages of agricultural development in Northern Africa through the analysis of the rich macro-botanical assemblages obtained from Ifri Oudadane, an Epipalaeolithic–Early Neolithic site from North-East Morocco. Results indicate the presence of domesticated plants, cereals (Hordeum vulgare, Triticum monococcum/dicoccum, Triticum durum and Triticum aestivum/durum) and pulses (Lens culinaris and Pisum sativum) in the Early Neolithic. One lentil has been dated to 7611 ± 37 cal BP representing the oldest direct date of a domesticated plant seed in Morocco and, by extension, in North Africa. Similarities in both radiocarbon dates and crop assemblages from Early Neolithic sites in Northern Morocco and the Iberian Peninsula suggest a simultaneous East to West maritime spread of agriculture along the shores of the Western Mediterranean. Wild plants were abundantly collected in both the Epipalaeolithic and the Early Neolithic periods pointing to the important role of these resources during the two periods. In addition to fruits and seeds that could have been consumed by both humans and domesticated animals, fragments of esparto grass (Stipa tenacissima) rhizomes have been identified. This is a western Mediterranean native plant that may have been used as a source of fibres for basketry.     

Wild or domesticated? An ancient DNA approach to canid species identification in South Africa's Western Cape Province

The fragmentary remains typical of archaeological fauna can prove impossible to identify when confronted with closely related sympatric species. In southern Africa domesticated dogs (Canis familiaris) and black-backed jackals (Canis mesomelas) pose just such a problem, rendering difficult an accurate assessment of the timing of arrival of domesticated dogs in the region. Contextual evidence has suggested that canid remains from four Later Stone Age archaeological sites were likely to be domesticated dogs rather than jackals, and were subjected to ancient DNA analyses to determine species. Every tested specimen proved to derive from black-backed jackals. These data provide not only an unexpected window on southern African prehistory, but also highlight the value in applying ancient DNA techniques to archaeological species identification.     

The Status Problem of Iberian Holocene Equids: New Data from Cueva de El Mirador (Sierra de Atapuerca,Spain)

Equid remains are scarce and very fragmented in Iberian Neolithic and Bronze Age sites. Evidence suggesting that horses were domesticated does exist, but it is often inconclusive, thus leaving the question unanswered. Today, DNA analyses have provided information about a, most likely Iberian, nucleus of horse domestication, making it crucially important to expand the database. The Holocene sequence (6^th and 3^rd millennium cal bc ) of the Cueva de El Mirador (Sierra de Atapuerca, Spain) has yielded 92 equid remains distributed in 12 Neolithic and Bronze Age levels. In this paper, we present the study of the equid remains from the Cueva de El Mirador from an archaeozoological perspective, giving indirect evidence of horse domestication. Our results vary widely and lead to open interpretations about horse domestication and how humans used these animals. This study of horse remains focuses on species identification and on determining how humans economically used these animals. Copyright © 2014 John Wiley & Sons, Ltd.     

The emergence of rice agriculture in Korea: archaeobotanical perspectives

This paper reviews archaeobotanical records on the beginning and spread of rice agriculture in the Korean peninsula. Argument for the earliest evidence of domesticated rice at the Sorori site, 15,000 years ago, is invalid. The evidence for rice cultivation in the Neolithic (Chulmun) is still insufficient although rice remains have been reported from a few late Neolithic sites in central-western Korea which dated to about 3000 BC. The existence of rice agriculture in the Bronze Age (Early and Middle Mumun: c.1300 ∼ 300 BC), on the other hand, is demonstrated by the high percentage and/or frequency of rice remains among crops recovered from various sites, as well as through the numerous findings of paddy fields. Rice appears to have been introduced from the Liaodong region, China, while so called 'southern diffusion route' that the beginning of rice cultivation was first stimulated by influences from Southeast Asia or South China is no more valid. Charred rice remains recovered from the Bronze Age dwellings consist of dehusked clean grains and weedy seeds are very rare among samples containing rice grains, which could be related with the harvesting and processing methods of rice. Measurements of charred rice grains also will be reported in this paper. Agricultural villages disappear from the archaeological records from the third century BC, which corresponds to the beginning of the Early Iron Age (Late Mumun), and reappear from the late first century with the emergence of urban societies.     

Ancient Iberian horses: a method to recover DNA from archaeological samples buried under sub-optimal conditions for preservation

Existing methods to extract, amplify, and sequence ancient DNA (aDNA) from horse bone and teeth were optimized to recover DNA from a depositional environment of highly permeable acidic soil. DNA was successfully retrieved using 0.10g of bone powder from horse (Equus sp.) remains dating to 25 K years utilizing the methods optimized for this archaeological material. The genetic analyses were performed in a facility that is dedicated to ancient DNA research (Paleo-DNA Laboratory, Thunder Bay, ON, Canada) and has not been previously used to analyse modern or ancient horse DNA. Research was replicated to obtain reliable sequencing results for six samples from the Iberian Peninsula that were consistent with published sequences of Equus caballus. The archaeological sequence data obtained support hypotheses that promote the significance that the Iberian Peninsula has had to the multi-focal centres of origin for horse domestication and distribution of modern horse breeds. The data presented may provide evidence of the existence of an Iberian refugium for Equus during the last glacial period, 10 K years BP. Further molecular data analyses will enhance the ideas presented by this data and our understanding of horse domestication and phylogeny. The optimization of molecular techniques to successfully obtain DNA using minimally destructive, cosmetically sensitive techniques from archaeological remains endeavours to foster further cooperation between museums and researchers.     

Recent Developments in the Archaeology of Southwestern China

Archaeology of ancient China’s periphery has traditionally been examined through the historiographic lens of Chinese textual sources. Social developments in the periphery are often explained in relation to accounts of migration from “core” regions of China. Setting conventional paradigms and textual sources aside, this article examines prehistoric developments in southwestern China in conjunction with broader trends in Southeast Asia. This comparative approach reveals that the development of bronze metallurgy in southwestern China parallels trends observed among Neolithic communities in Southeast Asia. Using recent data and a reassessment of radiocarbon dates for the Bronze Age, I propose that sociopolitical complexity emerged in southwestern China as part of a multiregional phenomenon that had its beginning with the formalization of trade networks during the Neolithic period.     

Marking Identity through Cultural Cranial Modification within the First Sedentary Communities (Ninth to Eighth Millennium BCE) in the Near East: Tepe Abdul Hosein,Iran

This paper explores bioarchaeological evidence for cultural cranial modification (CCM; a.k.a. headshaping) in early Neolithic Near East and at Tepe Abdul Hosein (TAH), Iran, in particular. Skeletal remains from 12 Neolithic individuals (n  = 12) recovered from eight mortuary contexts were analysed for evidence of intentional CCM. The TAH remains are among the earliest Neolithic human skeletal remains in the world, dated to around 10 000 years ago. Five adult individuals have sufficient cranial remains to allow assessment of CCM. Of the remaining seven individuals, three are foetuses or perinates, for which no in vivo CCM was expected to be observed, two are subadults with no assessible cranial remains, and two adults have no associated cranial remains. Of the five adult individuals, which could be assessed for CCM, four display circumferential CCM, instigated by the use of two bands. Three are male, while the sex of the fourth cannot be estimated. The fifth individual displays no modification and is female. While all the modified individuals that can be sexed at TAH are male, the sample size is too small to draw extensive inferences as to the potential connection of CCM with any gendered sociocultural practices. Other possible motivations for CCM at TAH include elaboration of status differentiation or group affinity, the former of which can most likely be ruled out based on current understandings of early Neolithic societies in the region. The extent of the two‐band circumferential modification at TAH is significantly more pronounced than at Ganj Dareh, a contemporaneous site located within the same watershed. The discovery of different extents of headshaping within the same watershed, in relatively close proximity to each other, has a bearing on the understanding of the first sedentary Neolithic communities in southwest Asia and the elaboration of bodily difference and identity. Copyright © 2017 John Wiley & Sons, Ltd.     

Ancient DNA analysis of desiccated wheat grains excavated from a Bronze Age cemetery in Xinjiang

Wheat has been one of the most important crop in Eurasia since the Neolithic period. Understanding the spread of wheat cultivation is crucial to understanding the spread of agriculture as a whole and the interactions between prehistoric populations across the Eurasian continent. However, the routes by which wheat cultivation spread eastwards have been poorly understood to date, due to the scarcity of plant remains recovered from archaeological sites. Desiccated wheat grains excavated from the Xiaohe cemetery in Xinjiang, and dated to the early Bronze Age, show excellent DNA preservation. Here we present an ancient DNA (aDNA) analysis of wheat (Triticum sp.) grains excavated from Xiaohe and provide the first definitive evidence for bread wheat in China during the Bronze Age. The nuclear ribosomal DNA internal transcribed spacer regions (ITS1 and ITS2) and the intergenic spacer region (IGS) were amplified. The IGS region within the D genome of wheat has a 71 bp insertion that is absent from corresponding regions in the A and B genomes. The results showed that the Xiaohe wheat showed most sequence similarity to hexaploid bread wheat (Triticum aestivum), including the characteristic insertion into the D genome. The presence of bread wheat at the Xiaohe cemetery is discussed in relation to it having spread into Xinjiang by the Bronze Age, providing new insight into the origins of bread wheat in East Asia.     

Feeding Management Strategies among the Early Neolithic Pigs in the NE of the Iberian Peninsula

The socio‐economic relevance of domesticated animals during the Early Neolithic in the Iberian Peninsula is indisputable, yet we essentially know little about the way they were managed. Among domesticated animals, pig (Sus domesticus ) was a common food source, and previous studies have shown the potential of stable isotopes for assessing variability in pig diet in relation to husbandry practices. Nevertheless, this approach has never been applied to the earliest pigs in the Iberian Peninsula. We analysed the carbon and nitrogen stable isotope composition of pig bone collagen from several Early Neolithic sites in the NE Iberian Peninsula. While pig δ¹³C values were similar across different populations, there were significant differences in δ¹⁵N values between sites. These are attributed to different pig husbandry systems, which may reflect distinct social and spatial organisation and interaction with environmental conditions during the Early Neolithic in this region. Copyright © 2017 John Wiley & Sons, Ltd.     

Chenopodium Seeds in Open-Air Archaeological Sites – How to Not Throw the Baby Out with the Bathwater

ABSTRACT

Differentiating between charred and uncharred plant remains may appear straightforward but for some taxa (here fat-hen, Chenopodium album type) can be very problematic. Recognition of the preservation state is obviously crucial for archaeobotanical data derived from dry, open-air sites. Fat-hen as a common weed, is also one of the most important components of a persistent soil seed bank. It is also a well-known food plant, gathered or cultivated. Numerous findings of fat-hen seeds in unclear states of preservation were noted in the Early Neolithic sites of the Linear Pottery culture in Kuyavia (N Europe). In previous studies such specimens were omitted as probably uncharred. Re-examination of Neolithic finds of fat-hen from that region showed the link of their abundancy with the earliest phases of the Neolithic occupation. The plant probably played an important role in the diet of the early Neolithic settlers there. It may indicate intensive use of local, open, fertile, probably alluvial areas. Distinguishing between ancient and modern specimens of that common weedy plant, producing large amounts of small, black, resistant seeds is thus very important, holding a great potential to shed new light on the origins of agricultural societies in this part of northern Europe.