首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 0 毫秒
1.
    
This journal published the first reported identification of Mycobacterium tuberculosis complex (MTB) DNA in ancient human remains but concerns were raised about the article two years after publication. These were based on methodology which, in the field of ancient DNA, was still developing. Here we present a re‐examination of the 1993 research conducted on three specimens which exhibited palaeopathologies indicative of tuberculosis. The specimens were: an ulna from pre‐European‐contact Borneo, a spine from Byzantine Turkey, and a lumbar‐sacral spine from 17th century Scotland. There was insufficient material to permit re‐examination of all of the original samples. The earlier results were confirmed in two independent laboratories using different methodologies. MTB DNA complex‐specific DNA amplicons were obtained, and sequenced in both laboratories, in a re‐analysis of samples which supported the earlier findings. Copyright © 2002 John Wiley & Sons, Ltd.  相似文献   

2.
The presence of DNA has been demonstrated in the cell nuclei of an ancient Egyptian mummy fragment. When extracted, this DNA proved to be degraded to a considerable extent and chemically modified. However, the preservation of nucleic acids in this specimen suggests that applying recombinant DNA techniques to the study of ancient mummified tissues might prove to be a fruitful future area of research.  相似文献   

3.
The polymerase chain reaction has been used to extract ancient DNA from a wide range of different types of material. We have considered the possibility of finding residual bacterial DNA in bone that may have been infected with Mycobacterium tuberculosis using this technique. We propose a method of collecting samples and testing for the presence of degraded genetic material in ancient bone. The steps of the polymerase chain reaction are detailed and discussed, as are those for the preparation of the bone sample. Results obtained would suggest that this technique could have wide application in osteoarchaeological research by giving us new information on diseases of antiquity. Future avenues for the investigation of bacterial DNA in ancient bone are suggested.  相似文献   

4.
A likely case of tuberculosis in an Iron Age human burial from Dorset, England is described. Osteological examination and biomolecular study support the diagnosis. A radiocarbon determination indicates a date range for the burial of BC 400–230. This case represents the earliest reported case of tuberculosis from Britain, and indicates that the disease was present here prior to the Roman invasion. Copyright © 2003 John Wiley & Sons, Ltd.  相似文献   

5.
    
The origin and evolution of the infectious disease tuberculosis (TB) and its pathogens is still not fully understood. An important effort for a better understanding of the underlying mechanisms of TB evolution lies within the investigation of skeletal and mummified material dating back several thousand of years. In this work, molecular data from mummified and skeletal material from different time periods of the Old World are compared, and the current status of ancient mycobacterial DNA analysis in ancient human remains is discussed, with particular reference to the genetic evolution of human TB. The molecular analysis of material from southern Germany (1400–1800 AD), Hungary (600–1700 AD) and Egypt (3500–500 BC) revealed high frequencies of TB in all time periods. In several individuals from ancient Egypt the mycobacterial DNA could be further characterised by spoligotyping. Thereby, evidence for ancestral M. tuberculosis strains was found in the pre‐ to early dynastic material from Abydos (3500–2650 BC), while typical M. africanum signatures were detected in the Middle Kingdom tomb in Thebes‐West (2050–1650 BC). Samples from the New Kingdom to Late Period tombs (1500–500 BC) were characterised as modern M. tuberculosis strains. In concordance with other studies on ancient skeletal and mummified samples, no evidence for the presence of M. bovis was found. These results contradict the theory that M. tuberculosis evolved from M. bovis during domestication, but supports the new scenario that M. tuberculosis probably derived from an ancestral progenitor strain. Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

6.
古代埃及官吏讲求说话艺术。他们追求的不是以美丽的辞藻说服对方,也不是以高超的辩术驳倒敌手,而是少说话、不到万不得已的时候不说话、说话时找准时候和分寸。对于以国王为代表的统治阶层来说,要求年轻的官吏们遵循这种说话艺术有利于维持由来已久的等级制和防止因言语不当而发生的矛盾和纠纷,对于初入仕途的年轻人来说,掌握说话的技巧和规则有助于他们理顺与上级和下级之间的关系,以免招致上司的厌恶和属下的不满。古代埃及说教文在培养年轻人以符合官场规则说话方面发挥了重要作用,而官吏的自传则揭示了他们身体力行说教文所灌输的劝诫的原因和目的。  相似文献   

7.
诞生于1822年的埃及学,是一门研究古代埃及的语言、文字、历史、文学、艺术、宗教、建筑和科技的综合性学科。埃及学发展至今已经有近200年的历史,但埃及学作为一门世界性的学科,对人类文明做出重要贡献是在20世纪以来逐渐取得的。经过数代埃及学家的共同努力,埃及学已有很大发展,古埃及文明是属于全人类的,日新月异的埃及学研究成果已成为全世界共同的文化财富。  相似文献   

8.
    
We report on a Mycobacterium leprae genome isolated from the remains of an individual with lepromatous leprosy that were excavated from a seventh‐century Hungarian cemetery. We determined that the genome was from a single nucleotide polymorphism (SNP) type 3K0 M. leprae strain, a lineage that diverged early from other M. leprae lineages. This is one of the earliest 3K0 M. leprae genomes to be sequenced to date. A number of novel SNPs as well as SNPs characteristic of the 3K0 lineage were confirmed by conventional polymerase chain reaction and Sanger sequencing. Recovery of accompanying human DNA from the burial was poor, particularly when compared with that of the pathogen. Modern 3K0 M. leprae strains have only been isolated from East Asia and the Pacific, and so these findings require new scenarios to describe the origins and routes of dissemination of leprosy during antiquity that have resulted in the modern phylogeographical distribution of M. leprae.  相似文献   

9.
The study of the DNA of ancient microorganisms in human remains represents one of the newest and most promising branches of molecular archaeoanthropology. Despite the growing number of papers addressing this subject, however, the analysis of ancient bacterial DNA is still a contentious issue. The indigenous microbiota of the gastrointestinal tract of man represents a community characterized by relative constancy in species composition and proportion. As a model system, we studied the preservation of the intestinal microbiota DNA in two naturally freeze-dried human mummies found on the Alps. This kind of mummy is an ideal subject for ancient DNA investigations. The first is a male body historically dated 1918 A.D. while the second is the famous Tyrolean Iceman (3.350–3.100 B.C.). The screening of bacterial 16S rRNA gene libraries from colon samples of the two mummies (49 clones for the 1918 mummy, 119 clones for the Iceman) showed that the characteristic composition of the intestinal microbiota of man (Alpha-, Beta-, Gammaproteobacteria, Bacteroides, Clostridia) is still kept in the library from the recent mummy while the Iceman's library is almost entirely composed by the DNA of clostridia. Comparison of the intestinal data with those from the literature describing the screening of 16S rRNA gene libraries from other parts of the Iceman's body and from permafrost specimens indicates that the changes in library composition may partly be attributed to the proliferation of clostridia inside the corpses, as described in forensic literature, and partly to the differential persistence of the DNA of gram-negative bacteria and endospore-former low-GC gram-positive bacteria. The present results contribute to the issue of the authentication of claims of pathogen DNA identification in archaeological human remains.  相似文献   

10.
    
Over the course of the last three decades, Ancient Egyptian scarabs have been discovered on Failaka Island off Kuwait, as well as at other sites in the Arabian Gulf. A scarab is the most expressive amulet of the cultural identity of ancient Egypt, revealing its influence and its cultural diffusion in the ancient world. Therefore, this paper discusses the reasons for the presence of such exotic products on the island and also answers the question about their origin. Were Failaka scarabs locally manufactured? What is their connection to the Levant? Are they a testimony to the interconnection between ancient Egypt and Arabia?  相似文献   

11.
    
Studies of the ancient history of infectious diseases have been facilitated greatly by development of a succession of novel analytical methods. In particular, laboratory analytical methods that are based on high‐throughput ancient deoxyribonucleic acid sequencing have received considerable attention in this respect. Even so, significant environmental caveats remain. There are many means by which microbes move through soil, often fairly readily. Thus, the depositional component of the postmortem environment, especially with respect to unshielded animal or human remains, is a fertile arena for many microbes that can contaminate archaeological specimens well after deposition and decay of soft tissues. The huge number of pathogenic and nonpathologic genera and species clearly dictate renewed interest and research into the long‐term biological activities of soil‐covered remains. In a tuberculosis context, we focus on various depositional concerns and limitations, such as contamination prior to archaeological discovery (perhaps many years prior), various means of microorganism movement in soil, the influence of these factors on differential diagnosis, and real hazards for misinterpretation of investigational results.  相似文献   

12.
    
A young pig skull, excavated from an ancient Egyptian village near Tell el‐Amarna, Middle Egypt, presents a number of unusual features which are suggestive of ante‐mortem human intervention with subsequent localised infection of the bone. Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

13.
This paper presents the debate over the presence or absence of keels in the construction of Egyptian ships of the dynastic period. It contains an analysis of ship representations and models dating to the 18th–20th dynasties of the New Kingdom (c.1539–1077 BC) that provide evidence for the introduction of keels in Egyptian shipbuilding, and new archaeological material from excavations at Thonis‐Heracleion that sheds more light on the question.  相似文献   

14.
    
An enormous earthquake in L'Aquila in 2009 brought to light human bodies buried in the underground rooms of the medieval St. John the Evangelist church (Casentino, Central Italy). Among the remains, we discovered a human fetus, whose post‐cranial bones were wrapped in bandages and cranial bones were reallocated inside a sort of hood. Anthropometrical investigation revealed an age at death of 29 ± 2 weeks of pregnancy for the little mummy. Radiograph analysis of the fetus showed that the skull was dissected and disconnected from the vertebral column, and the post‐cranial bones were completely disarticulated from the axial skeleton. The body was reassembled in a way of anatomic connection at a later stage. This mummified fetus dated to ad 1840 showed paleopathological evidence of a possible embryotomy and could be a rare and unquestionable case of embryotomy in archaeological context. Copyright © 2014 John Wiley & Sons, Ltd.  相似文献   

15.
    
The study of ancient DNA (aDNA) using molecular methods is an increasingly valuable tactic in bioarchaeology. While this method must be carefully undertaken to ensure that the molecules detected are representative of the ancient sample and not modern contaminates, there is a danger that a ‘one size fits all’ approach to validation will lead to misinterpretation and/or missed opportunities of valuable findings. When comparing human and pathogen aDNA, there are many shared technical means that can ensure best practice. However, there are a number of assumptions that should not be used for both scenarios. We discuss these aspects in reference to a recent article published by this journal and highlight some of the latest advances in molecular detection of ancient pathogen DNA that can further improve this endeavour. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

16.
The relief from the temple of Ramesses III (1184–1153 BC) in Medinet Habu is often considered to be the most ancient representation of a brailed sail. According to many researchers, the loose‐footed sail and the system of brails were invented in the ancient countries of the Syro‐Canaanite coast and were later adopted by the Ancient Egyptians. However, iconographic material from Ancient Egypt testifies to the indigenous origin of a loose‐footed sail. The article presents an unknown relief from the Amarna period (1352–1336 BC) that further supports this hypothesis.  相似文献   

17.
    
Systematic excavation of collective burial sites makes possible the recovery of skeletal series which may show bony evidence of infectious pathological conditions. This paper presents the first evidence of the existence of tuberculosis in prehistoric populations of NW Argentina with a subsistence economy based on agriculture and pastoralism. The study was carried out on individuals from Rincón Chico 21 cemetery, a burial site located in the Santa María Valley, Catamarca, used between the Late Ceramic Period and the onset of the Inca empire expansion (AD 1000–1400). Six individuals out of the 70 so far excavated showed destructive lesions in the vertebral bodies and periosteal reactions in other bones. The morphology and distribution of bone lesions led us to rule out several diseases from a broad spectrum of possible diseases that could have affected the skeletal system. Thus, the lesions were interpreted as caused by mycobacterial infections (Mycobacterium tuberculosis Complex). Considering previous studies on the dynamics of biocultural interactions which take into account information related from contextual associations and chronology, we can conclude that a tuberculosis‐like disease was present in prehistoric populations from NW Argentina. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献   

18.
    
We respond to issues raised in the recent Forum on “Ancient DNA and its contribution to understanding the human history of the Pacific Islands” in Archaeology in Oceania by Bedford et al. We first present an emerging model for the early peopling of Vanuatu combining the genetic and archaeological evidence. Second, we respond specifically to the criticisms of two contributors: Matisoo‐Smith and Sand. We discuss various misconceptions about the Teouma Lapita cemetery and about sampling issues in DNA research.  相似文献   

19.
20.
    
Microbial biofilms have developed on the surfaces and within the painted and gilded layers of mummy cartonnage at the Saqqara museum storeroom in Giza, Egypt. SEM–EDX, XRD and FT–IR–ATR techniques were applied to analyse the coloured and gilded materials, ground layer, textile support and binder used for the cartonnage. Aspergillus niger (24.8%), Penicillium chrysogenum (21.5%) and a novel cartonnage‐biodegrading bacterium, Bacillus sonorensis (23.7%), were the most abundant microbes growing over the cartonnage surface. In addition, Aspergillus tamari (15.4%), A. fumigates (8.1%) and Fusarium solani (6.5%) were identified. The pigments comprised Egyptian blue (cuprorivaite), cinnabar (red), orpiment (yellow) and green pigment made from a mixture of cuprorivaite and orpiment. Gold leaf was used for the gilded layer, calcium carbonate and gypsum comprised the ground layer, gum arabic was the binding medium and the fibre base was a fine linen textile. Microbial colonization tests were performed on aged cartonnage replica samples made from linen and pigments of similar composition to ancient pigments found in the cartonnage. Each sample was inoculated separately with A. niger, P. chrysogenum and B. sonorensis. Yellow orpiment samples were the exception, as no colour change was detected after colonization by the examined micro‐organisms.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号