Information obtained from the microscopic examination of cultural striations in human dentition

The surfaces of teeth from some fossil remains and archaeological samples have been examined by SEM, in order to study different types of cultural striations and to determine the archaeological information that these striations can provide. Different patterns of cultural striations are present in human samples from different sites and periods. Similar cultural striations can be interpreted in different ways, depending on the tooth in which they are present, their location in the tooth and their direction, regularity, number and width. The study of cultural striations can yield valuable information about different aspects of feeding behaviour, oral hygiene, patterns of cultural diffusion and manipulative work with teeth of past human groups. A close integration with other anthropological and archaeological data is needed and specific interpretations should be made in each case.     

Ancient bacterial DNA (aDNA) in dental calculus from archaeological human remains

The purpose of this study was to identify reactive bacterial aDNA in archaeological human dental calculus. Dental calculus was collected from a middle/late Neolithic human skull from Hulbjerg passage grave, Langeland, Denmark and prepared for transmission electron microscopy (TEM) or gold-labeled antibody TEM. TEM showed calcified, as well as non-calcified bacteria. Immunogold labeling occurred over the cytoplasmic portions of the sectioned bacteria. The result demonstrated that it is possible to identify aDNA sequences from bacteria in archaeological material of considerable age by this technique.     

Metamorphosis of root dentine and age

Transparency in the location of the tooth root apex is a normal phenomenon in human ageing and provides important information for determining a person's age. The illumination of root dentine may prove useful when trying to obtain an approximate estimation of a person's age, particularly when no other data can be obtained. Regression analysis could provide a reasonably reliable means of determining an approximate age of human remains and could be used in archaeological investigations. © 1997 John Wiley & Sons, Ltd.     

The Relationship Between Molar Wear and Age in an Early 19th Century AD Archaeological Human Skeletal Series of Documented Age at Death

Crown height of molar teeth was measured in a 19th century Dutch skeletal collection of known age at death. Results indicate an approximately linear relationship between crown height and age. A closer relationship between age and crown height exists for the mandibular than the maxillary molars. The mandibular molars wear at a faster rate than the maxillary molars. There is no evidence for any difference in wear rates between first, second and third molars. No effect on wear rate of ante-mortem loss of occlusal partner nor of dental caries could be detected. The rate of wear in the study material was markedly slower than that which characterizes most archaeological groups and the burials are drawn from a rather heterogeneous urban population; these factors mean that dental wear ageing would be expected to perform less well in this material than for most archaeological groups. Despite this, strong correlations were observed between molar wear and adult age at death; that this was the case despite circumstances unfavourable for its use supports the idea that dental wear is a reliable ageing technique for most archaeological skeletal material.     

Recording dental caries in archaeological human remains

Dental caries is an important condition to record in archaeological collections, but the way in which recording is carried out has a large effect on the way in which the results can be interpreted. In living populations, dental caries is a disease that shows a strong relationship with age. Both the nature of carious lesions and their frequency change with successive age groups from childhood to elderly adulthood. There is also a progression in the particular teeth in the dentition which are most commonly affected and, in general, the molars and premolars are involved much more frequently than the canines and incisors. Lower teeth are usually affected more than upper, although the condition usually involves the right and left sides fairly equally. In the high tooth wear rate populations represented by many archaeological and museum collections, there is a complex relationship between the form of lesions and the state of wear, which adds yet another range of factors to the changing pattern of caries with increasing age. In the same populations, chipping, fracture and anomalous abrasion of teeth are also common, and these contribute similarly to the distribution and forms of carious lesion observed. Amongst the living, the pattern of ante‐mortem tooth loss is important in understanding caries and, in archaeological material, there is also the complicating factor of post‐mortem tooth loss. Finally, there is the question of diagnosis. There are diagnostic problems even in epidemiological studies of living patients and, for archaeological specimens, diagenetic change and the variable preservation of different parts of the dentition add further complications. For all these reasons, it is difficult to define any one general index of dental caries to represent the complete dentition of each individual, which would be universally suitable for studying a full range of collections from archaeological sites or museums. Variation in the nature of collections, their preservation, tooth wear, and ante‐mortem and post‐mortem tooth loss mean that when such a general index appears to differ between sites, there could be many other reasons for this, in addition to any genuine differences in caries incidence and pattern that might have been present. It is suggested here that the best approach is instead to make comparisons separately for each tooth type, age group, sex, lesion type and potential lesion site on the tooth. Copyright © 2001 John Wiley & Sons, Ltd.     

Taphonomy,life history,and human exploitation of Rhinoceros sinensis at the Middle Pleistocene site of Panxian Dadong,Guizhou, China

Rhinoceros remains are commonly found in Chinese Pleistocene archaeological localities. This study examines the characteristics of the Rhinoceros sinensis sample from Panxian Dadong, a karst cave in the mountains of western Guizhou province, with a mammalian fauna in association with stone artefacts and human remains from the late Middle Pleistocene (MIS 6‐8). The distribution of skeletal elements shows a predominance of foot (metapodial and phalanges) and lower limb (carpals and tarsals) bones, while the dental age‐at‐death profile, constructed using dental eruption and tooth wear data, is characterised by a high frequency of prime age adult teeth. There is little taphonomic evidence for the involvement of non‐human carnivores or natural agencies in the formation of the faunal assemblage. Instead, it appears that human activities were responsible for the unexpected prevalence of prime age adults. Copyright © 2008 John Wiley & Sons, Ltd.     

A longitudinal study of tooth growth in a single individual based on long- and short-period incremental markings in dentine and enamel

Ground sections of teeth from a single individual revealed approximately 30 fluorescent lines in the dentine resulting from successive courses of tetracycline antibiotics given over a period of approximately 12 years. In addition, many teeth showed long-period incremental markings in the dentine. Short-period daily cross-striations in the enamel were used to calculate the periodicity of the long-period incremental lines in both enamel and dentine. The incremental markings in the dentine were then used to calibrate the time of administration of each of the courses of tetracycline antibiotics. Enlarged micrographs of the teeth, constructed using fluorescence microscopy, were used to measure the increase in height of different teeth throughout the growth period on each occasion the tetracycline antibiotics were given. The times of initial mineralization of the teeth and the duration of crown formation were also determined for each tooth section. Line plots of tooth height against age were computed and used to calculate the rate of increase in tooth height during crown and root formation for one example of each tooth type. Initial rates of increase in height during enamel apposition and coronal dentine formation were fast, between 10 μm and 14 μm per day. During the latter phase of crown formation and during early root formation, tooth height increased at a slower rate of between 4 μm and 6 μm per day. Rates of increase in tooth height then rose to between 13 μm and 26 μm per day during the second half of root formation before falling off to a rate of approximately 4 μm per day during root completion (as the root apex formed). The times of initial crown mineralization and the total crown formation times estimated in this individual were all within the range known for modern humans but there was comparatively early mineralization of the third permanent molar. These findings are discussed in the context of other studies on human dental development.     

Determining Age and Season of Death by Use of Incremental Lines in Norwegian Reindeer Tooth Cementum

Using incremental patterns in tooth cementum is a powerful tool for age assessment. Recent developments have shown that the method has a large potential as an indicator of the season of death of the individual in question. In this paper, the results of a study comparing thin sections of teeth of the modern Hardangervidda reindeer population to those of Iron Age, eleventh, and thirteenth century reindeer hunting stations from the same area are presented. The tooth wear stages were compared to the actual age of the individual (based on the number of incremental lines and the age at eruption). It was established that in the studied reindeer populations wear stages sometimes result in too low an age estimate. Having identified the start of the period of deposition for rest lines in the modern reindeer population as early autumn and winter, analysis of the outermost layer in the tooth cementum at the archaeological sites points to a prolonged hunting season in the thirteenth century, while in the earlier phases hunters were probably present on fewer and shorter visits.     

Age estimation by tooth/pulp ratio in canines by peri-apical X-rays: reliability in age determination of Spanish and Italian medieval skeletal remains

Estimation of age at death is an unavoidable step in the process of human identification, both in forensic practice and in the anthropological and palaeopathological study of skeletal remains. In several cases, in which medical or demographic records are completely lacking, a reliable estimation of the age at death becomes very important. Skeletal remains from archaeological contexts suffer from several biasing factors such as post-mortem changes, taphonomy and various burial practices depending on age, sex and social status of the deceased persons.     

Incremental banding in dental cementum: Methods of preparation for teeth from archaeological sites and for modern comparative specimens

Analysis of incremental banding in dental cementum is a well-established means of determining the age and season of death of wild mammals. The dental cementum of domesticated mammals likewise can indicate age and season of death. Methods of preparation applicable to archaeological teeth differ from those used for modern specimens, however, and this paper describes two methods that have given excellent imaging on teeth of Bos taurus; one for modern teeth and the other for teeth from archaeological sites.     

Estimating age by tooth wear of prehistoric human remains in Brazilian archaeological sites

The occlusal surfaces of 298 permanent maxillary and mandibular molar teeth of prehistoric shellfish‐gatherer subjects from the Piaçaguera and Tenorio sites (4930 to 1875 BP), near the central‐northern coastline of São Paulo, Brazil, were examined for classification of macro‐wear stages. Molar tooth wear is an indication of masticatory activity and can be used in the estimation of age at death. The examination of visual and schematic aspects of occlusal macro‐wear used a visual chart proposed by Brothwell, which includes the three superior and inferior, left and right, permanent molars. Three examiners performed the macroscopic observations twice under the same conditions. The resulting age estimates were compared with previous information of age estimated by skeletal examination. A reduced intra‐ and inter‐observer variation was observed; all re‐examinations indicated discrepancies of less than two years for the upper and lower limit of the age range estimates. The procedure was also considered consistent with the skeletal method used for age estimation of human remains excavated in Brazilian archaeological shell mounds, with a discrepancy of less than 8.22 years between the upper and lower limits of estimates by both methods. Age classification by the occlusal molar wear may be a useful tool for the classification of archaeological findings, mainly when only fragmentary skeletal remains are excavated. The current results indicate that the application of the Brothwell chart for Brazilian archaeological series presented satisfactory results of consistency, and its expanded use may represent a relevant adjunct for research. Copyright © 2006 John Wiley & Sons, Ltd.     

Malocclusion and methodology: The problem and relevance of recording dental malalignment in archaeology

The study of incorrectly positioned teeth has a long history, especially in relation to human groups and domestic dogs. Dental and orthodontic specialists have been particularly concerned to establish ways of recording and comparing such anomalies. However, Colyer and others have established that anomalous dental positioning occurs in a wide variety of species, and might at times even have had adaptive advantage. In the case of dogs, some modern varieties clearly have severe malocclusion problems, while others are little affected. Because it has been suggested that early domesticated dogs already display some degree of dental crowding or malpositioning, it seems important to consider the methodology for recording such anomalies. It is suggested that a quick and easy method of recording and comparing tooth positioning is by angular measurements (preferably taken on standard photographs of occlusal views). In the British archaeological dog samples selected, it was found that tooth malpositioning (and thus malocclusion of upper and lower dentitions) was only slight, compared to the overall occurrence of such anomalies in modern dogs. It is suggested that this kind of simple procedure may be of value in the investigation of secular trends in the frequency of malpositioning and malocclusion of dogs, if not other species, through time.     

Absolute age and tooth eruption and wear sequences in sheep and goat: determining age-at-death in zooarchaeology using a modern control sample

This paper seeks to improve our understanding of the relationship between mandibular tooth eruption and wear sequences and absolute age in modern sheep and goat. While the relationship between tooth eruption and wear and absolute age has been investigated for primitive breeds in parts of the Old World, our pilot study extends the applicability of this research to modern breeds and to another geographic region (North America). A modern control sample of two species of domestic caprines – Ovis aries (sheep) and Capra hircus (goats) from Manitoba, Canada – are used to evaluate the two major systems for analyzing mandibular tooth wear and eruption (Payne and Grant) and for assigning an absolute age-at-death. In order to compare the two systems, an absolute age for Grant's Mandibular Wear Stages was established for the first time, previously demonstrated only for Payne's system. The analysis confirmed that there is a very high correlation between the known age and estimated age based on tooth eruption and wear. However, Payne's system generated far coarser age-at-death profiles, which lowers its utility in constructing harvest profiles. Grant's system is recommended for ageing be utilized since it can yield more sensitive age estimations. In conclusion, it is possible to utilize the modern caprine breeds to establish the relationship between tooth eruption and wear and absolute age to reconstruct age and season of death of archaeological samples.     

Daily rates of dentine formation in macaque tooth roots

When tetracycline antibiotics are administered either orally or by injection they bind at the forming mineral front of the dentine and become incorporated into teeth as a permanent label. In this study, oxytetracycline was used as a label in order to investigate the growth of root dentine in macaques. There are no good data available in the literature for daily rates of root dentine formation over a prolonged period of time in either humans or macaques. Records of ten doses of oxytetracycline administered orally at irregular intervals (but at the same time of day) over a period of 10 months to a young rhesus macaque monkey (Macaca mulatta) were used to calculate the rate of dentine formation in tooth roots. Rates of dentine formation in the first portion of third permanent molar root formation, and in the apical portion of permanent canine and premolar root formation, were consistently between 3 μm and 4 μm per day. There was no evidence in this study of a gradual decrease in daily dentine formation rate from the crown to the root, or along the length of a dentine tubule through the bulk of the dentine, as has been implied previously. With the exception of faster rates recorded in cusps or slower rates recorded at the very beginning or very end of primary dentine secretion in other studies, the results of the present study suggest a consistent rate of dentine formation in permanent macaque teeth. What data there are for human dentine formation rates suggests that it is likely this finding can be extended to human dentine as well.     

Trauma and pathology of a buried dog from San Nicolas Island,California, U.S.A.

To date more intact dog remains have been found on San Nicolas than on any of the other seven California Channel Islands. However, little is known about them. During the 2007 summer field season we excavated a medium sized young male dog in a flexed burial position from a pit at CA-SNI-25, a large Native American village site. The dog exhibits hypodontia, noticeable tooth attrition, severe scapular trauma, and vertebral, rib, and phalanx pathologies. Most of the injuries appear to have resulted from a severe blunt force trauma of unknown origin and it is likely the dog would not have survived without human care. The results of our analysis contribute to the, somewhat limited, published data on archaeological dogs in California. In this paper we describe the biological characteristics of the dog especially his anomalies and pathologies and compare them with published reports of other North American archaeological dogs with similar conditions.     

Strontium isotope analysis of human tooth enamel from Barsinia: a late antiquity site in Northern Jordan

The archaeological site of Barsinia represents a model of a mixed subsistence strategy in the late antiquity of Jordan. Contrary to historians' belief that the late antiquity economy was stagnated, archaeological evidence at the site of Barsinia points to wealth accumulation as mirrored by the local wine industry and trade. As the economic growth may enhance population dynamics, the study tests the population mobility at the site using strontium isotope ratios from the human tooth enamel. The study comprised 12 right upper third molars and 12 rodent teeth samples. The results confirm that all of the sampled individuals were local to the area (raised in the area) and whose diets were probably obtained from spatially restricted localities in the region.     

A comparison of different non-destructive methods of age estimation in skeletal material

The skeletal remains of unidentifiable persons, recovered in recent years from the churchyard adjoining the House of Correction in Oslo, included 91 instances in which the maxillae and/or mandibles had been preserved, and these were examined. The length of the apical translucent zone in unsectioned teeth and the amount of secondary dentine deposit have been regarded as two of the most reliable factors in odontological age estimation. This study has used two methods, each using one of these factors, on single-rooted teeth from, respectively, 78 and 76 individuals. The distribution of age at death, as estimated from the two dental methods and from anthropological criteria, was then compared with the distribution of age at death of 380 individuals recorded in the church register for the House of Correction. Age calculations from secondary dentine, measured indirectly on dental radiographs of premolars, and estimates based on anthropological criteria both seemed to approximate to the chronological age distribution better than age estimates based on the length of the apical translucent zone. Statistical analyses indicated that all three methods of age estimation were significantly different (p<0.05). When compared with the church register, each method assigned a lower percentage of individuals to the younger and older age-groups and a higher percentage to those in the middle. No statistical difference could be found between the age distribution from the church register and estimates from either anthropological criteria or dental radiographs; but when age estimates based on these two latter methods were compared, 39.5 per cent differed by more than 10 years.     

Rates of dentine mineralization in permanent human teeth

Previous experimental studies have estimated linear rates of dentine formation in modern humans to be close to 4μm day⁻¹. In this study a method similar to that first adopted by Kawasaki, Tanaka and Ishikawa⁵ was used to estimate linear rates of dentine mineralization over a period of 1200 days in both the cusps and cervical regions of several permanent tooth types. All teeth were from the same individual. Rates in the cusps of teeth with the tallest crowns were estimated to be between 5 μm day⁻¹ and 6 μm day⁻¹. This is higher than previous estimates in permanent tooth crowns, although rates in the cusp of a first permanent molar, where cusps were less tall and cuspal dentine therefore less thick, were close to previous estimates of 4 μm day⁻¹. Despite this variation in cuspal rates, mineralization rates were linear in all cusps studied over a long period of time. Rates in the cervical region, either close to the enamel dentine junction or to the cement dentine junction, were estimated to be between 1.3 μm day⁻¹ and 1.5 μm day¹¹, much slower than reported previously. Rates in the mid-portion of the dentine, in both the lateral part of the crown and in the cervical one-third of the root, rose steadily to match rates in the cuspal region, but then slowed towards the pulp chamber. These data extend the findings of previous studies on permanent human dentine. They demonstrate a wide range of mineralization rates in permanent dentine and provide a more secure basis for judging different rates in different locations of different human tooth types.     

Age at death in cattle: methods,older cattle and known-age reference material

Abstract

New work is presented regarding the estimation of age at death in cattle based on the teeth. For younger cattle, before all the teeth are fully in wear, mandible stages are based on the eruption events, subdivided using wear on the most recently erupted tooth. For older cattle, a method of study using the position of the cement-enamel junction and the root arch of the molar teeth is presented. These are used to define summary mandible stages for older cattle. In order to suggest the ages at which the mandible stages occur, studies were made using reference material of known age at death at the Julius Kühn Museum, Halle, Germany (88 records), and two smaller collections in the UK (6 records). Further reference data were obtained from study of the literature (42 data points) (Jones and Sadler 2012). The results are presented using standard summary methods and the more detailed mandible stages.     

Estimation of age at death of sika deer (Cervus nippon) from an archaeological site based on radiographs of mandibular molariform tooth development

The development of permanent premolar and molar teeth was examined in mandibular radiographs from wild sika deer (Cervus nippon) of known ages (74 males, 66 females, 4 unknown; age range 1–54 months) from Hyogo Prefecture. Tooth development was scored according to the method described by Brown & Chapman (1991a, b). Stage‐specific scores for all molar and premolar teeth were summed, and simple cubic regression analysis was used to analyse the relationship between the total score and age in months. Our analysis showed a strong cubic correlation between the total score and chronological age in months. The equation obtained from this analysis was used to estimate age within a 95% prediction interval in seven archaeological specimens from the Asahi archaeological site in central Japan. Copyright © 2010 John Wiley & Sons, Ltd.