Identification of fragmentary bone from the Pacific

We explore bone microstructure for taxonomic identification of archaeological bones too fragmentary to permit secure identification on morphological grounds. Backscattered electron (BSE) imaging is used to observe bone tissue types and the arrangement of vascular canals, and to facilitate quantification of osteonal canal dimensions. Examination of known examples of relevant taxa (humans [n = 8], pigs [n = 4] and dogs [n = 4]) shows significant differences among them. When the results of this examination are applied to a blind test of modern and archaeological specimens (humans [n = 8], pigs [n = 2]), 100% of specimens are identified correctly. The approach is applied to 13 morphologically unidentifiable fragments from Hawai’i and Fiji to evaluate its potential for identifying bone tools and to increase the number of samples available for dietary analysis. Potential applications of the approach for other contexts are discussed.     

The geochronological potential of isoleucine epimerisation in cassowary and megapode eggshells from archaeological sites

Our research demonstrates that the extent of isoleucine epimerisation (A/I) in fragments of avian eggshells provides geochronological information in archaeological contexts. In the archaeological sequence of Hay Cave, northern Queensland, Australia, there is an excellent correspondence between the A/I values of Australian brush-turkey (Alectura lathami) eggshells (n = 99) and independent geochronological control (n = 16 radiocarbon ages including 4 on eggshell calcite). The A/I values identify three phases of deposition during the Holocene at Hay Cave. In contrast to the Alectura eggshell A/I values, a poor correspondence was observed between the A/I values of cassowary (Casuarius) eggshells from Toé (n = 35) and Kria caves (n = 23) (Ayamaru Plateau, Papua) and the depths from which the specimens were recovered in these stratified sequences. Given coherent archaeofauna trends and radiocarbon chronologies (n = 8 and 2 eggshell calcite radiocarbon ages at Toé and Kria, respectively) with respect to depth, the variable A/I values are not explicable in terms of mixing. Rather, the variability is most likely due to exposure of the eggshells to the high temperatures of campfires. Despite the variability, eggshells with relatively low A/I values amongst specimens recovered from similar depths (and therefore presumably least influenced by high temperatures) exhibit a gradual increase in A/I with respect to depth, as expected in a stratified deposit. From this observation it is suggested that the identification of heated eggshells will increase confidence in geochronological information provided by A/I. These studies illustrate the complications that arise from campfire-induced acceleration of amino acid racemisation and emphasise that although this phenomenon is common, it is not universally encountered in archaeological contexts.     

Possible shell beads from the Middle Stone Age layers of Sibudu Cave,South Africa

Recent excavations at Sibudu Cave, KwaZulu-Natal, South Africa, uncovered an Iron Age horizon below which is a complex 3 m thick Middle Stone Age sequence with post-Howiesons Poort, Howiesons Poort, Still Bay and pre-Still Bay layers. Available OSL ages indicate that the Howiesons Poort occupation is older than 60 ky and the Still Bay older than 70 ky. Here we present the archaeological context and the taphonomic analysis of six Afrolittorina africana, three of which bear perforations, from the Still Bay and Howiesons Poort layers of this site. The single specimen from the latter cultural horizon comes from the lowermost layer attributed to this technocomplex. This and the depositional context of this layer suggest that this shell derives, as do the other five, from the Still Bay occupation layers. Taphonomic analysis of the archaeological specimens based on present day Afrolittorina africana biocoenoses, microscopic examination, morphometry, experimental perforation of modern shells, and a review of the natural agents that may accumulate marine shells at inland sites, indicate probable human involvement in the collection, transport, modification, and abandonment of Afrolittorina africana in Sibudu. If confirmed by future discoveries these shells would corroborate the use of personal ornaments, already attested at Blombos Cave, Western Cape Province, by Still Bay populations. The apparent absence of ornaments at Howiesons Poort sites raises the question of the mechanisms that have led to cultural modernity since it seems to contradict the scenario according to which cultural innovations recorded at Middle Stone Age sites reflect a process of continuous accretion and elaboration interpreted as the behavioural corollary of the emergence of anatomically modern humans.     

New radiometric ages for the Early Upper Palaeolithic type locality of Brno-Bohunice (Czech Republic): comparison of OSL,IRSL, TL and C dating results

New radiometric data are reported from the recent excavation of the type locality of the Early Upper Palaeolithic entity of the Bohunician. Recently obtained radiocarbon (¹⁴C) data on charcoal are compared with new Optically Stimulated Luminescence (OSL) dating of sediment. OSL ages were determined on sediment from the archaeological occupation at Brno-Bohunice, as well as from the over- and underlying loessic sediments. Multiple techniques were applied, which all gave congruent results. While a dual protocol (post IR-OSL) failed the quality criteria tests, ages were obtained by Multiple-Aliquot-Additive-Dose (MAAD) on polymineral material and Single-Aliquot-Regeneration (SAR) on fine grain quartz extract as well as on polymineral material. Fading tests show significant loss of Infrared Stimulated Luminescence (IRSL) after storage for 3 and 12 months for one sample, but little or no fading for others. The resulting (uncorrected) age estimates are smaller than those on quartz by OSL methods. The latter are considered to be more reliable estimates of the sedimentation age of these deposits. The measured OSL doses do not show a simple distribution and the lowest 5% was used for age calculation to represent the most likely sedimentation age. The quartz from the loess overlying the archaeological layer is OSL dated to 30.9 ± 3.1 ka, while the sediment for the paleosol which contains the archaeological layer gave an age of 58.7 ± 5.8 ka. The attribution of this paleosol to the Hengelo interstadial is therefore questionable. However, if the Hengelo interstadial is correlated with the Dansgaard/Oeschger (D/O) event 12, statistical agreement within 2-σ is achieved. The OSL result for the archaeological layer is in accordance with a weighted average TL date on heated flint artifacts of 48.2 ± 1.9 ka from this layer as well as calibrated radiocarbon data (CalPal Hulu 2007) from nearby locations. However, radiocarbon data on charcoal samples obtained during excavation at Brno-Bohunice 2002 provide age estimates between 30 and 40 ka ¹⁴C-years, which translate to approximately (33) 35–44 ka on the calendric time scale according to the Hulu 2007 model. For the underlying loess a depositional age of 104.3 ± 10.6 ka was obtained by OSL. The presented OSL ages indicate that a simple correlation of soil sequences between sites within a region has to be verified by chronometric dating.     

Dating North Asian surface assemblages with ostrich eggshell: implications for palaeoecology and extirpation

East Asian ostrich (Struthio anderssoni Lowe) was thought to have become extinct sometime in the Late Pleistocene. Petroglyphs portray ostrich with Pleistocene animals, and ostrich eggshell (OES) fragments and ornaments from Holocene sites were considered to be older fossils. Here we summarize previous radiometric dates for ostrich eggshell (OES) and present 15 new calibrated accelerator mass spectrometry (AMS) dates, indicating that ostrich survived in Mongolia and northern China until at least 8.9 ka BP. The dates in our sample population suggest a correlation between Struthio and warm steppe environments, with extirpation probably related to essential changes in Holocene steppe ecosystems including human expansion. This study assesses the usefulness of Asian OES for archaeological dating by AMS, constrains the date of Asian ostrich extirpation, and investigates palaeoenvironmental implications of ostrich survival and extinction.     

Examining dispersal mechanisms for the translocation of chicken (Gallus gallus) from Polynesia to South America

DNA sequencing and radiocarbon dates from the archaeological site of El Arenal-1 (Arauco Peninsula, south central Chile) suggest that Polynesians may have introduced chicken (Gallus gallus) to South America prehistorically (A.D. 1300–1420). The Chilean specimen identified has the exact same amplified mtDNA pattern as samples recovered from archaeological sites in Samoa and Tonga, but is only one base different from prehistoric chicken samples from Tonga (n = 1), Niue (n = 1), Easter Island (n = 1 of 6), and Hawai'i (n = 1). To examine the possible dispersal mechanisms of chicken between Polynesian islands and the Americas, we conducted computer simulations of seafaring from these six island groups and the Tuamotus, as well as return voyages. Simulation results from sailed vessels suggest that in certain months, landfall on the Pacific Coast of South America from Samoa and Tonga was fairly successful at around 40% (particularly in March for the former and January/February for the latter). For Niue, sailed vessels were 21% successful in January with only 2% success in December. Sailed vessels from the Tuamotus (a proxy for closer island groups to the west with evidence for chicken such as the Cook Islands) were successful in January and December, with 15% and 23% of the vessels making landfall in South America. Vessels sailing from all simulations heading east from Easter Island resulted in landfall. Hawai'i had only a 1% success rate if sailing in January, but high numbers of vessels reached southern Mexico and Central America in August and September. Most landfalls occurred much farther north than Chile, in Ecuador and Peru. Unsuccessful voyages from all of the islands other than Easter Island and Hawai'i often made landfall on islands to the east. Return voyages from Chile were not possible or extremely rare during most times of the year. Overall, seafaring simulations suggest that Polynesians may not have initially landed in close proximity to El Arenal-1—contemporaneous or even earlier archaeological evidence of translocated chicken remains may indeed be found further north. In addition, if Polynesians found it difficult or impossible to return to their homeland, the possibility exists that co-mingling between Polynesians and South American groups resulted in offspring that carried an admixture of genetic traits.     

A pilot study into the effects of X-ray and computed tomography exposure on the amplification of DNA from bone

This research investigates the intersection of radiography and aDNA, two commonly used methods in bioarchaeology. The goal of this project was to investigate the effects of radiation on the ability to amplify DNA from bone. Bones (n = 124) from domestic pig (Sus scrofa) feet were randomly sorted into a control group and four treatment groups: (1) single exposure X-ray; (2) single exposure CT; (3) multiple exposures X-ray; and (4) multiple exposures CT. Number of PCR cycles required to amplify DNA in 100 bp, 200 bp and 400 bp segments were used as a proxy for the amount of available DNA. In the 200 bp CT sample, distal phalanges required significantly more amplification cycles than did the other bones and were removed from analysis. Results suggest that in general radiation exposure fragments DNA thereby decreasing the amount that is amplifiable. While these results are suggestive, further research is required to elucidate the degree to which radiation fragments DNA in archaeological specimens.     

Sedimentological and archaeological fabrics in Palaeolithic levels of the South-Eastern Pyrenees: Cova Gran and Roca dels Bous Sites (Lleida, Spain)

The comparative study of sedimentary fabrics in relation to archaeological fabrics in various levels of the sites of Cova Gran de Santa Linya (Middle and Early Upper Palaeolithic) and the Roca dels Bous (Middle Palaeolithic) has made it possible to analyse the formation dynamics of the deposits and determine the extent of disturbance of the archaeological levels by natural processes. To achieve this, diagrams and two- and three-dimensional indices were calculated from the azimuth and dip angle of natural clasts and artefacts. The results indicate that the sedimentary levels were formed mainly by planar fabric shape gravitational processes (low depositional angles), which differ notably from the archaeological fabrics they contain, characterised by a greater degree of isotropy. This difference in the fabrics would reflect human activity and indicates that the archaeological levels both in Cova Gran and Roca dels Bous have not been subject to significant natural modifications, and are preserved in situ.     

Stable isotope evidence of human diet at the Nukdo shell midden site,South Korea

Carbon and nitrogen stable isotope analysis was undertaken on bone collagen extracted from archaeological human (n = 48) and animal (n = 45) skeletons from the Nukdo site, Location I C, South Korea. This shell midden and grave site is dated from the late Mumun (550–300 BC) to early Iron Age (300 BC-1 AD) periods. The herbivorous mammals fell within the range of C₃ consumers, with average values of δ¹³C = −21.0 ± 0.5‰ and δ¹⁵N = 3.6 ± 0.5‰ for deer (n = 16) and δ¹³C = −20.6 ± 0.5‰ and δ¹⁵N = 4.5 ± 2.0‰ for wild boar (n = 17). Humans from this site averaged δ¹³C = −18.3 ± 0.4‰ and δ¹⁵N = 11.2 ± 0.7‰ for adults (n = 15) and δ¹³C = −18.7 ± 0.7‰ and δ¹⁵N = 12.5 ± 1.1‰ for juveniles (n = 33). These δ¹³C values indicate that there was no significant input of C₄ plants in the human diets and this may be associated with the spread of rice agriculture in the Mumun period. Human bone collagen δ¹³C and δ¹⁵N values indicate that there was some consumption of marine foods, although the main protein sources were from terrestrial foods. The isotope data demonstrate that the humans at Nukdo had mixed diets that included marine and terrestrial protein, including C₃ plants such as rice. Finally, the isotope results from the juveniles indicate that weaning occurred before the age of 1.5 years in this period.     

Stable isotopes and diet at Ancient Kerma,Upper Nubia (Sudan)

Carbon and nitrogen stable isotope values were measured from bone collagen extracted from archaeological Nubian human (n = 54) and faunal (n = 61) populations from the site of Kerma, Sudan. Collagen suitable for isotopic analysis was extracted from 22 faunal and 48 human samples from the Eastern cemetery site, dated to the Middle Kerma (c. 2050–1750 BC) and Classic Kerma (c. 1750–1500 BC) periods respectively. The isotopic data indicate that the human dietary regimen included a mix of C₃ and C₄ plant-derived components, with a larger C₄ component than previously reported in archaeological Egyptian Nile Valley populations. Elevated δ¹⁵N values are attributed to consumption of dietary resources from a ¹⁵N-enriched terrestrial ecosystem. The faunal isotope data also indicate the consumption of C₃ and C₄ plants. The large range of δ¹³C values measured in both the human and faunal samples supports previous work suggesting that a significant portion of the populations buried at Kerma may have originated elsewhere, further confirming the Nile Valley as a major corridor for population movement in the region.     

Why did the ancient inhabitants of Palmyra suffer fluorosis?

The skeletal remains uncovered from the 2nd and 3rd century underground tombs of Palmyra, Syria, retain traces of arthritis and mottled enamel. A brown discoloration was also observed in the teeth. In order to clarify that these facts can be related to fluorosis, the teeth excavated from Tomb C and F in the Southeast Necropolis were analyzed. The fluoride contents of the fluorosis-suspected teeth (0.56 ± 0.16% (n = 7)) were higher than those of normal teeth (0.16 ± 0.18% (n = 7)). The highest content (0.85%) proved that 22% of the hydroxide in apatite was substituted by fluoride. The fluoride concentrations of the natural water including deep well water available today in this area ranged from 0.3 to 3.0 mg l⁻¹. The highest fluoride concentration was found to be regulated by the dissolution equilibrium of fluorite, CaF₂, through the process of evaporation and concentration in this arid region. Because of the common ion effect by calcium ions in a high concentration, the fluoride concentration was lower than that expected from the solubility product of fluorite (8.2 mg l⁻¹). The mechanism of this process has probably remained unchanged since ancient times and therefore the drinking water used by the ancient people of Palmyra was estimated to contain fluoride at a level causing fluorosis.     

The Quaternary fossil record of the genus Testudo in the Iberian Peninsula. Archaeological implications and diachronic distribution in the western Mediterranean

This paper explores the presence of tortoise remains and its meaning in Palaeolithic archaeological contexts. We focus on two topics: (1) the presence or absence of the genus Testudo from the Early Pleistocene to the Holocene in SW Europe; and (2) the relationship between Testudo and the palaeoclimatic and palaeoenvironmental conditions diachronically. In order to address this objective, we collected published and unpublished data from different sites on the eastern Iberian Peninsula. The results obtained by this study demonstrate that Testudo hermanni was the only tortoise present in this area until recent times and its presence in archaeological sites is almost always related to anthropic activities. Finally, the disappearance of Testudo from archaeological sites in some areas is related to climatic changes and events that occurred during the last stages of the Pleistocene.     

Lower Pecos and Coahuila peyote: new radiocarbon dates

Peyote, a psychoactive cactus native to the Chihuahuan Desert, has been preserved from excavations at only two archaeological sites: Shumla Caves in the Lower Pecos region of southwest Texas and shelter CM-79 near Cuatro Ciénegas in Coahuila, Mexico. We determined three indistinguishable radiocarbon ages of 5160 ± 45, 5200 ± 35, and 5210 ± 35 ¹⁴C years BP, yielding a mean age of 5195 ± 20 ¹⁴C years BP for the three specimens from Shumla Caves. For one of the Cuatro Ciénegas specimens we obtained the first direct radiocarbon date of 835 ± 35 ¹⁴C years BP. This study demonstrates the use of peyote by inhabitants of the Lower Pecos region of the Chihuahuan Desert about 6000 calendar years ago, and confirms its use by inhabitants of the Cuatro Ciénegas region of the Chihuahuan Desert in Late Prehistoric times. The Shumla Caves' specimens are composed of an aggregate of ground peyote mixed with other plant material, i.e., they appear to be manufactured peyote effigies, and are definitely not intact peyote buttons.     

Estimation of Age,Growth and Fishing Season of a Palaeolithic Population of Grayling (Thymallus thymallus) Using Scale Analysis

The fish remains sampled from archaeological sites are generally the result of human food refuse; therefore, the study of retrieved fish scales may provide reliable information on the season of capture and on paleoclimate and paleoenvironmental changes. Among Western European freshwater fishes, the grayling, Thymallus thymallus ¸ has the most easily recognizable scales and is a commonly recovered species from Palaeolithic contexts. This study, therefore, is based on the growth pattern analysis of modern and archaeological scales from grayling specimens. The modern specimens were collected monthly in France (n  = 22), Switzerland (n  = 16), Finland (n  = 20) and Sweden (n  = 10). Scale growth patterns were measured using numerical analysis to a high accuracy. The results showed that the archaeological population of grayling is closer to the present Finnish population. Furthermore, the comparison of seasonal growth patterns between current and archaeological European populations demonstrated that graylings were normally captured during the spring at Le Taillis des Coteaux Magdalenian site. This type of approach can be used to understand environmental conditions at a low spatial scale and also to help identify fishing seasons during archaeological periods. Copyright © 2017 John Wiley & Sons, Ltd.     

Late Holocene human impact on the vegetation around Mizorogaike Pond in northern Kyoto Basin,Japan: a comparison of pollen and charcoal records with archaeological and historical data

Pollen and macroscopic charcoal analyses of AMS radiocarbon-dated sediment from Mizorogaike Pond, located near Japan’s ancient capital established in AD 794, were used along with archaeological and historical data to reconstruct vegetation change in the northern Kyoto Basin since 7300 cal yr B.P. Between ca. 7300 and 3400 cal yr B.P. (Early to Late Jomon period), the site was surrounded by warm-temperate forest composed of Quercus subgenus Lepidobalanus and Q. subgenus Cyclobalanopsis with Celtis/Aphananthe trees. With the occurrence of fire disturbance, Q. subgenus Lepidobalanus increased from ca. 3400 to 1400 cal yr B.P. (Late Jomon to Kofun periods). In the early seventh century (Asuka period), Pinus started to increase, coinciding with a significant charcoal peak, probably related to the operation of roof tile kilns near the site. Pinus continued to increase and Q. subgenus Cyclobalanopsis decreased through the seventh to tenth centuries (Asuka to Heian periods). Further increase of Pinus occurred in the eleventh century, possibly reflecting the establishment of the manor of Kamigamo Shrine. From the eleventh to seventeenth centuries (Heian to medieval periods), no significant vegetational change or fire disturbance took place. In the eighteenth century, the landscape became totally open, with poor vegetation and sparse Pinus woodland. The medieval and early modern landscapes reconstructed from the palaeoecological record are rather similar to those described from studies of ancient artwork and historical documents. This study demonstrates that late Holocene vegetation change in the northern Kyoto Basin was closely tied to anthropogenic activities, such as the pottery industry and fuel wood collection.     

Identifying sword marks on bone: criteria for distinguishing between cut marks made by different classes of bladed weapons

Swords have been one of the major weapons used in violent conflicts for much of human history. Certain archaeological situations, especially those dealing with the recovery and analysis of battle casualties, may raise questions about what type(s) of bladed weapon was used in a particular conflict (e.g., the battle of Kamakura, Japan, AD 1333; the battle of Wisby, Sweden, AD 1361; the battle of Towton, England, AD 1461). Little work has been done, however, on developing criteria to differentiate sword cut marks from other types of cut marks, or to distinguish between marks created by different sword types. To develop such criteria, bovine tibiae (n = 7) were struck using six different types of bladed weapon and the resulting marks (n = 92) were analyzed. Eight traits describing the morphology of the cut mark – such as shape, the presence and unilateral/bilateral state of flaking and feathering, the presence of bone shards, associated breaks, etc. – are defined and related to blade type used. Sword marks were found to be easily distinguishable from knife marks. The variation in marks made by different sword types is significantly correlated with differences in blade weight (p < 0.0001), grip (p < 0.0113), and sharpness (p ≤ 0.0179). The criteria and analyses developed and implemented in this study will be of use to researchers in forensics and osteoarchaeology who want to infer bladed weapon type from marks on bones.     

The Kapthurin Formation revisited

This contribution reports on the results obtained from the 1980–7 research in the Kapthurin Formation (Baringo, Kenya) which aimed at the refinement of the chronology, stratigraphy and environmental evidence in order to establish a framework for the interpretation of the prehistoric sites. In 1966 a Late Acheulean industry with prepared-core technique and blade component, dated betweenca 0.82/0.64 myr and 0.23 myr had been described in the area concerned. All the sites belong to the middle Kapthurin formation which is situated in the Bruhnes normal polarity chron and has a lower limit of 0.58 myr. The upper limit of 0.23 myr could not be confirmed. The fauna is consistent with the available dating evidence. The sites are situated in a flat savanna landscape which was built up by a braided river system under slightly more humid conditions than the present climate. On some of the sites artifacts were found in association with palaeosoils. Although the interpretation of such assemblages must consider the impact of natural turbation processes, the palaeosoils provide exceptional conditions for comparing sites from the same phase of landscape stabilization. The present archaeological evidence suggests a sequence of Late Acheulean and Post-Acheulean (Sangoan?, Middle Stone Age) assemblages rather than the presence of one single industry as described previously. The relation between these assemblages and the two mandibles ofHomo sp. (aff.erectus) BK67 and BK8518 (found in 1966 and 1982) emphasizes the need for more research in the area.     

Short-chain n-alkanes (C16–20) in ancient soil are useful molecular markers for prehistoric biomass burning

The incorporation of plant biomass into soil usually leads to long-chain n-alkanes with a relative predominance of odd carbon numbered homologues. Contrastingly, an increase in short-chain even carbon numbered n-alkanes was found in charred biomass with progressing temperatures. We applied lipid analysis to buried ancient topsoils that contained charred organic matter and to corresponding control soils, which were characterized by a lighter color and lower contents of charred materials. Commonly, the proportion of lipid extracts was found to be lower in the ancient soil than in the control samples, which indicated an enhanced degradation of organic matter, e.g., by thermal degradation. All samples displayed a particular pattern of short-chain and even carbon numbered n-alkanes (maximum at n-C₁₆ or n-C₁₈). The ratios CPI (carbon preference index) and ACL (average chain length) for the investigated soil samples matched the ratios found for maize and rye straw charred at 400 °C or 500 °C, respectively. These molecular ratios indicate the presence of charred biomass. The predominance of short-chain and even carbon numbered n-alkanes was a result of thermal degradation of biomass. The degradation products were preserved in ancient soils and could be applied as molecular markers in archaeological or palaeoenvironmental research.     

Morphometric analysis of limpets from an Iron-Age shell midden found in northwest Portugal

The present work reports an analysis of a shell midden found in Terroso hillfort, an important Iron-Age oppidum located in northwest Portugal. The midden was dated from the latest phase of occupation of the hillfort, between the Ist century BC and the Ist century AD (Roman period), and contained 684 well-preserved Patella shells. The identification of archaeological shells was carried out comparing them with modern specimens collected in two shores in the neighborhood of the hillfort. The identification of modern shells was based on radula pluricuspid teeth and shell morphology. Relative abundance of Patella species in the Terroso midden was different from modern populations. Archaeological shells were dominated by Patella vulgata, but Patella intermedia was the most abundant species in modern populations. Dimensions of archaeological shells were very different from modern populations. Shell length range and variability in archaeological shells were lower than in the modern populations. Log height vs. length plots for archaeological shells were different from modern populations. Patella shells from Terroso midden was significantly taller then modern specimens. These differences between archaeological shells and modern populations could be due to environmental changes, namely an increase in wave action and intensity. These harsher costal conditions could have resulted from an alteration in the morphology of the coast, caused by a rise in the mean sea level from 2000 years BP to the present. Alternatively or additionally, the observed differences can be related to gathering strategies. Archaeological shells could have been selected by size, and collected preferentially at high shore and in sheltered sites.