A strontium and oxygen isotope assessment of a possible fourth century immigrant population in a Hampshire cemetery,southern England

The aim of the project was to test the hypothesis, using oxygen and strontium isotopes, that a group of burials in the Late Roman cemetery of Lankhills, Winchester, southern England, were migrants from the Danube region of central Europe. The method assumes that the oxygen isotope composition of immigrants from this locale would be significantly more depleted that any one British origin and that the restricted range in Sr isotope compositions produced by chalk in the overlying biosphere of southern England would discriminate between the local population and settlers from elsewhere. As a control for the immigrant group a sample of Romano-British individuals were examined to provide a comparative data set. The results showed that the majority of the individuals used to define the “local” control group plotted in a restricted field of strontium and oxygen isotope composition that was consistent with the values expected for the Hampshire area of southern England. By contrast, the “exotic”, putatively immigrant population generated a much more dispersed field including four with δ¹⁸O drinking water values of −10‰ or less, which supports a non-British origin for these individuals. The study shows that the archaeological data suggesting that there is an exotic population buried at the Lankhills cemetery is generally supported by the isotope work, although the “exotic” group appears to a rather dispersed set of individuals rather than a single population from a restricted overseas location.     

Spatial variation of biologically available strontium isotopes (Sr/Sr) in an archipelagic setting: a case study from the Caribbean

This paper presents the results of strontium (Sr) isotope analysis of modern and archaeological animal remains and modern plant samples from the Caribbean region. The goal is to assess if patterns of human mobility can be determined from the archaeological record in an archipelagic setting. The range of variability and spatial distribution of biologically available strontium isotope signatures is reported and data evaluated to determine if sufficient heterogeneity exists to permit the identification of mobility despite the presence of potentially large contributions of marine strontium in island and coastal ecosystems. The (is)landscape is divided into several sub-regions based on the age and lithology of underlying geology and the variability of ⁸⁷Sr/⁸⁶Sr ratios is reported as; mean ⁸⁷Sr/⁸⁶Sr ± 2 standard deviations, number of samples: 1) Volcanic and Intrusive rocks (0.7077 ± 0.0019; n = 162); 2) Cretaceous–Miocene Limestone (0.7085 ± 0.0009; n = 50); 3) Pliocene–Quaternary Limestone (0.7091 ± 0.0004; n = 54); 4) Sedimentary Deposits (0.7094 ± 0.0015; n = 16); 5) Metamorphic Deposits (0.7104 ± 0.0014; n = 6). There are substantial differences between expected ⁸⁷Sr/⁸⁶Sr values based on associated geology and measured ⁸⁷Sr/⁸⁶Sr in flora and fauna samples. These differences emphasize the importance of focusing on bioavailable strontium, as opposed to geological proxies, for estimations of Sr isotope ranges, especially in archipelagic environments. While some overlap exists between the ⁸⁷Sr/⁸⁶Sr ranges of certain sub-regions, other sub-regions possessed limited variation and could be distinguished based on strontium isotope data. This spatial patterning of biologically available strontium indicates that strontium isotope analyses can be successfully applied to the archaeological study of human mobility within the Caribbean region.     

Reappraisal of ‘chronospecies’ and the use of Arvicola (Rodentia,Mammalia) for biochronology

The water vole, genus Arvicola, is characterised by a broad geographic distribution throughout Europe and is widespread during the late Middle and Upper Pleistocene. This genus is used as a major biostratigraphic tool within the Quaternary. Specific determinations using the Schmelzband-Differenzierung-Quotient or SDQ have identified many chronospecies within the fossil species Arvicola cantiana (Hinton, 1910). As SDQ calculation remains limited, this study reappraises the Arvicola genus in terms of morphodiversity and morphospace using outline analysis which takes into account the tooth as a whole. Outline analysis suggests that one single species of Arvicola, A. cantiana, was present during the Pleistocene. This species shows great variability with no trends or patterns in morphospace. Thus, these results call into question the reliability of SDQ for specific determinations and throw doubt on the biochronological framework based on Arvicola.     

Stable isotope analysis of dog,fox, and human diets at a Late Holocene Chumash village (CA-SRI-2) on Santa Rosa Island,California

Stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope analyses of dog (Canis familiaris), island fox (Urocyon littoralis), and human bone collagen from CA-SRI-2 (AD 130–1830) on Santa Rosa Island, California provide a proxy of diet and the relationships between humans and these animals. Carbon isotopic signatures indicate that Native Americans and their dogs at CA-SRI-2 subsisted almost exclusively on marine resources, while the island fox ate primarily terrestrial foods. Nitrogen isotopes and archaeofaunal remains indicate that humans and dogs also ate higher trophic level foods, including finfishes, marine mammals, and seabirds with smaller amounts of shellfish. The CA-SRI-2 island foxes appear to have eaten higher amounts of terrestrial foods, similar to the diets observed in modern fox populations. These data generally confirm the commensal relationship assumed to exist between domesticated dogs and people, but the carbon isotopic composition of dogs is enriched ∼2‰ compared to humans. We hypothesize that the difference in carbon isotopes between dogs and humans may have resulted from a higher consumption of C3 plants with lower δ¹³C values by humans, or less likely from the ingestion by dogs of significant amounts of bone collagen, which is enriched by ∼4‰ over associated muscle.     

In situ oxygen isotope micro-analysis of faunal material and human teeth using a SHRIMP II: a new tool for palaeo-ecology and archaeology

A Sensitive High Resolution Ion MicroProbe (SHRIMP II) has been used to make high spatial resolution in situ micro-analyses of oxygen isotopes in fish otoliths, and teeth from fossil herbivores and a Neanderthal. Large intra-tooth variations in the oxygen isotopic composition (up to 9‰) were observed in the enamel of herbivores from the Neanderthal fossil site of Payre, consistent with preservation of seasonal cyclicity. The range of isotopic compositions observed in Neanderthal tooth enamel was much smaller (∼3‰), possibly the result of a longer enamel maturation time averaging out variability. An archaeological otolith from a Preceramic site in Northern Peru exhibited marked changes in δ¹⁸O over life, due either to the fish occasionally migrating from the sea to a lower salinity habitat, or to short-lived rises in sea water temperature. A fish otolith from Australia's Willandra Lakes World Heritage Area showed clear seasonal variations, but also a general trend towards isotopically heavier and more saline water, as indicated by higher δ¹⁸O and Sr/Ca values resulting from increased evaporation. The results of these case studies are compared to results of oxygen isotope analysis using more conventional methods and demonstrate the ability of the SHRIMP II to provide precise high spatial resolution in-situ oxygen isotope analyses of a variety of biogenic materials. This approach has major advantages over conventional methods. It can provide rapid, micro-scale isotopic analyses of sub-permil precision without the need for chemical preparation of the sample.     

Endangered species,archaeology, and stable isotopes: huemul (Hippocamelus bisulcus) isotopic ecology in central-western Patagonia (South America)

The management and conservation of animal species should be based upon a long-term analysis that considers its geographical distribution and feeding patterns. The study of carbon and nitrogen stable isotopes on skeletal remains provides a quantitative approach to the paleodietary reconstructions and constitutes a potent tool for comparing behavioral aspects of the fauna. In this paper we present the first set of isotopic values for modern and archaeological samples of Huemul (Hippocamelus bisulcus) from the forests of Central-western Patagonia, Chile. Contrary to initial expectations, our analysis indicates that there is no evidence of an important incidence of the canopy effect on the δ¹³Ccollagen values, which we suggest is due to an ecological selection that drives the huemul to focus its predation on open sectors within the forest. On the other hand, a wide range of δ¹⁵Ncollagen values for huemul was verified. This could reflect the N impoverishment of the forest soils and provide an interesting ecological indicator. The long-term information offered by the archaeological record provides the necessary context for decision-making conducive towards the preservation of Hippocamelus bisulcus in Patagonia.     

Oxygen isotope seasonality in a temperate estuarine shell midden: a case study from CA-ALA-17 on the San Francisco Bay,California

Seasonality determination using stable oxygen isotope (δ¹⁸O) analyses in archaeological mollusk shell has been largely limited to aquatic settings where one of the two factors that control shell δ¹⁸O – water δ¹⁸O (or salinity) and temperature – is assumed to be constant. Open coastal marine environments reflect the former situation, and tropical estuaries constitute the latter. In an effort to expand stable isotope seasonality to an ecological setting where neither variable remains constant, we present a model of annual shell δ¹⁸O cycle of aragonite deposition derived from instrumental data on salinity and temperature from San Francisco Bay, California. The predicted range of modeled shell δ¹⁸O is consistent with observed δ¹⁸O values in prehistoric and modern shells when local conditions are considered. Measurements of δ¹⁸O taken at 0 mm and 2 mm from the terminal growth margin were made on 36 archaeological specimens of Macoma nasuta from a late Holocene hunter-gatherer site CA-ALA-17, and season of collection was inferred using the shell δ¹⁸O model. We conclude that shellfish exploitation occurred through the year with the exception of fall, which may indicate scheduling conflicts with acorn harvesting or other seasonally abundant resources elsewhere. The model supports the feasibility of stable isotope seasonality studies in temperate estuaries, provided that instrumental records are available to quantify the relevant water conditions at appropriate spatial and temporal scales.     

Alkaline cooking and stable isotope tissue-diet spacing in swine: archaeological implications

In this study we examine the effects of alkaline cooking on carbon and oxygen stable isotopic ratios of mineralized tissues from nine pigs raised on monotonous mixed C3/C4 vegetarian diets. Two sources of collagen (humerus and mandible) and two sources of apatite (humerus and enamel) were analyzed. Within each diet group, humerus and mandible collagens were found to record equivalent δ¹³C and δ¹⁸O ratios; however, enamel apatite was found to be enriched over bone apatite by 2.3‰ in carbon and 1.7‰ in oxygen. Alkaline cooking was found to slightly, but significantly increase the Δ¹³C_{collagen-diet} and Δ¹⁸O_{collagen-diet} of bone collagen. A similar trend towards enrichment was observed in bone and enamel Δ¹³C_apatite-diet and Δ¹⁸O _apatite-diet, but the differences were not significant. Observed isotopic shifts were consistent with increased nutrient utilization of the alkaline-cooked maize as compared to raw maize. In addition, a reexamination of the relationship between diet and tissue carbon isotopic values suggests that species and alimentary type should be considered when interpreting ancient diets.     

Chemical characterisation of anthro-technosols from Bronze to Middle Age in Bologna (Italy)

The aim of this research was the understanding of a peculiarly expanded soil sequence in Bologna (i.e., Roman Bononia, Etruscan Felsina), one of the most important archaeological poles for Etruscan colonisation and settlement in northern Italy. The uppermost part of the chronological sequence dating from the Bronze Age up to medieval and modern times was analysed for the first time from geochemical and pedological viewpoints. The integration of archaeological, stratigraphical and pedological data supported by soil chemical analysis, a fundamental key for reconstructing a correct evolutionary sequence, made it possible to recognise seventeen buried Soil Units (ancient Technosols, Anthrosols and Cambisols) characterised by different kinds and degrees of intensity of anthropogenic influence. In some cases, the results show a relatively severe element concentration (Cu, Sn) mainly characterising the Iron Age (Etruscan and Villanovan) stratigraphic units, representing an ancient industrial pollution linked to local dispersal of melting activity dump products generated in near-site surrounding areas. Therefore, a comparison was possible between natural blank and anthropogenically polluted geochemical values for some local soils.     

Reassessing the northern limit of maize consumption in North America: stable isotope,plant microfossil,and trace element content of carbonized food residue

In combination, the analysis of carbonized food residue for stable C and N isotopes, elemental composition, and plant microfossils (phytoliths and starch granules) offers a powerful tool for understanding patterns of prehistoric maize (Zea mays) consumption in small-scale societies. Using this approach, we conclude that maize was more widely consumed in North America than previously suspected. Between ∼AD 700 and 1600, despite little or no archaeological evidence of gardening, corn was a widespread component of diet on the eastern Canadian prairies. This pattern, furthermore, extended into the adjacent boreal forest at about the same time. However, carbon (δ¹³C) and nitrogen (δ¹⁵N) values on food residue samples vary widely, suggesting significant overall dietary differences from one region to the next. Analysis of a subset of residue samples for elemental composition (using ICP-OES [inductively coupled plasma–optical emission spectrometry]) may help identify broad trends in the provenience of foods consumed at these sites.     

“Mycenaean” political domination of Knossos following the Late Minoan IB destructions on Crete: negative evidence from strontium isotope ratio analysis (Sr/Sr)

Strontium isotope ratio analysis of human dental enamel and bone is applied to investigate a highly debated question of population movement and cultural discontinuity in Prehistoric Aegean Archaeology. The Late Minoan IB (ca. 1490/1470 BC) destructions on Crete are succeeded by cultural upheaval. The novel cultural features that appear at Knossos (Crete) in this period have forerunners in the Mainland. In Cretan context, the Linear B writing system, the funerary architecture and burial practices of the Mainland style are interpreted as evidence of an actual “Mycenaean” long-term settlement and political domination of Knossos. Human skeletal material from tombs that are associated with non-locals from the Mainland based upon the material culture is analysed to measure ⁸⁷Sr/⁸⁶Sr. The results of the analysis show that all the examined individuals from the Knossos tombs were born locally.     

Integrated seismic tomography and ground-penetrating radar (GPR) for the high-resolution study of burial mounds (tumuli)

The study primarily aims at providing adequate imaging resolution of large and prominent targets of archaeological interest, such as pyramids and tumuli, at all depth levels. We implemented an integrated seismic tomography and georadar (STG) technique to perform high-resolution imaging and characterization of tumuli (burial mounds). We tested the proposed technique on a preserved late Bronze Age burial mound in northern Italy, for which STG succeeded in performing an accurate 3-D reconstruction of the structure and stratigraphy as proved by later archaeological excavations. We completed two transmission seismic tomography measurements, at present ground level and at 1.5 m elevation, with a 24-channel seismograph and 15° angular separation between geophones. The ground-penetrating radar (GPR) dataset encompasses 12 250 MHz radial profiles and 12 common mid point gathers for velocity analysis. Shallow layers of the mound are successfully imaged by GPR, whilst the structure of the deep central part of the tomb is reconstructed from seismic traveltime inversion. In particular, GPR images lenses and layers of sediments forming the external part of the tumulus, evidences of a looting attempt, peripheral structures associated with later exploitation of the mound (furnaces) and, in the external sector of the tumulus, the top of the deep layer of silty sediments covering the funeral chamber. Tomographic results reveal seismic velocity anomalies of potential archaeological interest at ground level, which were successively validated by archaeological excavations. The integration of GPR and tomographic datasets is an effective strategy to overcome the imaging and interpretation problems related to the structure of such peculiar funeral monuments. STG can be applied to a virtually unlimited dimensional range and requires a limited data acquisition, processing and inversion effort. The results of the study allowed the identification of the funeral chamber and a detailed imaging of layering and structural details.     

Disentangling long bones of foxes (Vulpes vulpes and Alopex lagopus) from arctic archaeological sites

To discern the presence of two anatomically close species within an archaeological assemblage is always a problem for the analyst. A particularly interesting case is the distinction between the red fox (Vulpes vulpes) and the arctic fox (Alopex lagopus), which is often determined using non-rigorous criteria and producing results that must be regarded with caution. Classical methods for separating these two species and determining sexual dimorphism, and eventually the sex ratio, such as analyses using uni- and bivariate plots, are based on arbitrarily fixed limits between sexes. In this text, a more robust statistical method termed mixture analysis (MA) is used to determine the species of limb bones from foxes. First, the MA is applied to a sample of each species using metric data taken from a collection of present-day fox skeletons. Afterwards, the MA is applied to archaeological samples dated to the Dorset period and retrieved from the Tayara site (near Salluit, South Hudson Strait, Nunavik). The results clearly demonstrate that the greatest length (GL) of long bones, especially the humerus and tibia, is the best measurement for distinguishing the species, followed by the distal breadth and the proximal breadth; and that GL is better suited for sexing foxes. The results of MA applied to the Tayara site collection lead to the conclusion that the red fox is present along with the arctic fox among the assemblage, and that there is no fox body size change during the last two millennia in the Eastern Arctic.     

Estimating meagre (Argyrosomus regius) size from otoliths and vertebrae

This study presents a method for predicting meagre (Argyrosomus regius) body size (total length) from otoliths and vertebrae recovered from archaeological sites. The method involves regression equations calculated from a reference collection of 36 meagre skeletons and 113 meagre otoliths (sagitta) and allows the simultaneous estimation of original body size and minimum number of individuals (MNI) from archaeological bone structures. We selected the following measurements to predict meagre body size: greatest dorso-ventral height, greatest mediolateral breadth, and greatest anteroposterior length of the vertebrae centra; maximum anteroposterior length, medial anteroposterior length, and dorso-ventral height of the sagitta. Our results show that the original body size of meagre can be accurately predicted from many bone measurements (r² range: 0.921–0.992). We exemplify the use of the regressions in the assessment of size variation and MNI of meagre from four Portuguese Mesolithic sites. We show that regression results provide additional insight into the significant role that this fish played in the subsistence of coastal fisher-hunter-gatherers, who targeted medium-sized animals but were also capable of acquiring rather larger specimens.     

Irrigated taro (Colocasia esculenta) farming in North Kohala,Hawai‘i: sedimentology and soil nutrient analyses

How did traditional farming transform the natural environment in the Hawaiian Islands? This question is one that has largely been addressed for rainfed farming of crops like sweet potato (Ipomoea batatas), but evidence is lacking for irrigated farming of the critical staple crop taro (Colocasia esculenta). We describe the results of soil nutrient and sedimentological analyses of deeply-stratified pondfield deposits representing a 600-year-long record of irrigated taro farming in the North Kohala District, Hawai‘i Island. Soil is categorized by particle size to determine modes of transport and deposition, and concurrent soil nutrient analyses were conducted to infer shifts in the source of sediments and changes associated with taro harvesting. The advent of farming is clearly detectable in sedimentology, the presence of charcoal found within sediments, and soil chemistry. However, diminished nutrient concentrations can be attributed largely to deposition of a mixture of upstream sediments. Overall, there is no clear evidence for nutrient draw-down by taro harvesting, but we cannot yet rule it out as a factor. This study demonstrates the inherent difficulty of correlating changes in soil nutrients evident in irrigated pondfields with the long term history of soil nutrient cycling.     

Mesolithic shellfish exploitation in SW Italy: seasonal evidence from the oxygen isotopic composition of Osilinus turbinatus shells

Oxygen isotope analyses were carried out on the topshell Osilinus turbinatus from archaeological sites with the aim to investigate the temporal exploitation patterns by Mesolithic groups inhabiting coastal caves in SW Italy. In order to assess the present day O. turbinatus intra-annual δ¹⁸O variability, living specimens were collected monthly at Marina di Camerota (SW Italian peninsula). Their shell-edges were analysed and the seasonal results were compared with archaeozoological material. Our findings show that Mesolithic O. turbinatus exploitation was carried out almost exclusively during the colder and intermediary seasons (e.g. autumn, winter, spring), with very sporadic harvesting during the warmer seasons. Results suggest a possible regional exploitation pattern of O. turbinatus by Mesolithic groups inhabiting this region.     

The Temple of Venus (Pompeii): a study of the pigments and painting techniques

We report here on a study of 57 fragments of wall painting excavated from the Temple of Venus (Pompeii). These samples were characterised by a wide range of analytical methods. Data showed that the palette is varied, although not so broad as that found in other buildings in Pompeii, and is consistent with pigments used elsewhere in Pompeii and in the Roman Empire. It is composed of: natural earths, minerals and rare artificial pigments. Paintings are made up of thin paint layers (0.01–0.10 mm thick) strongly adhering to the underlying preparation layer. Nonetheless, in some cases thicker layers (up to 0.40 mm) were recognised, often spread on other previous painting layers. Samples were also compared with the microstratigraphic criteria developed in Piovesan (2009) to distinguish wall painting techniques. This comparison demonstrated that both fresco and lime painting techniques were adopted.     

Paleodietary analysis of a San Francisco Bay Area shellmound: stable carbon and nitrogen isotope analysis of late Holocene humans from the Ellis Landing site (CA-CCO-295)

The late Holocene archaeofaunal record of the San Francisco Bay Area demonstrates temporal declines in the abundance of low-cost, high-ranked marine and terrestrial resources. During later periods of occupation, faunal assemblages are often dominated by lower-ranked, higher-cost resources, suggesting an increase in diet breadth through time. Archaeological resource intensification models argue that this marks a late Holocene decline in foraging efficiency in the Bay Area, driven by human-induced harvest pressure. This study examines dietary change in the region using stable carbon and nitrogen isotope data from 65 human burials, spanning two temporal components (cal AD 55–890 and cal AD 762–1550) at the Ellis Landing site (CA-CCO-295). The strong linear relationship between collagen carbon and nitrogen isotope values reflects both marine and terrestrial food consumption, with individuals showing a high level of dietary variability at the site. No temporal trend or meaningful sex differences were found in isotope values. The widening of diet breadth predicted by the archaeofaunal record occurred prior to the occupation of the Ellis Landing shellmound site. The trend in exploiting a greater amount of terrestrial resources during the Middle and Late Period in central California was supported by isotopic evidence.     

A short note on starch and xylem of Colocasia esculenta (taro) in archaeological deposits from Pitcairn Island,southeast Polynesia

We present results of starch analysis of archaeological deposits from Pitcairn Island. High concentrations of starch grains preserved in cell membranes, and xylem tracheary elements, consistent with introduced Colocasia esculenta (taro) were found. Because of limited age control, we are uncertain if the microfossils are prehistoric. Problems associated with identifying taxa with small starch grains in extractions from weathered deposits are highlighted.     

Locally diagnostic phytoliths of wild rice (Zizania palustris L.) from Minnesota,USA: comparison to other wetland grasses and usefulness for archaeobotany and paleoecological reconstructions

Wild rice (Zizania spp.) is an important native grain of north-central North America, with spiritual and subsistence significance to many native peoples. Due to lack of suitable proxies, its past distribution and pattern of use are poorly known. This study demonstrates the diagnostic value of silica phytoliths from Z. palustris as a proxy tool to detect its past occurrence in a variety of paleoenvironmental and archaeological contexts. Our main findings are: 1) Dominant wetland grass species in Minnesota produce phytoliths distinct from Zizania phytoliths; 2) Closely related Leersia oryzoides produces a few Zizania-like phytolith morphotypes; however, these potential confusers can be identified based on 2-D and 3-D morphologies; 3) Z. palustris produces a wide variety of phytolith morphotypes, many of which are only found in certain parts of the plant (e.g., Inflorescence Type 1 from the spikelet); 4) Z. palustris produces 23 locally diagnostic phytolith morphotypes that can be used to determine the presence and abundance of wild rice in modern and paleo lake sediments; and 5) Wild rice phytolith Inflorescence Type 1 is the morphotype most likely to be observed in archaeological contexts, and may be unequivocally diagnostic for the genera Zizania.