The persistence of caffeine in experimentally produced black drink residues

Black drink is a native North American caffeinated tea produced from the leaves of Ilex vomitoria. The beverage was an important part of prehistoric Southeastern culture, as it was the only native source of caffeine in North America. The leaves of I. vomitoria were, therefore, a potentially valuable trade resource during the period. As such, the ability to identify archaeological black drink residues is useful to Southeastern archaeologists. In order to determine the plausibility of detecting I. vomitoria in residues, black drink was repeatedly produced in experimental unglazed pottery. The resulting potsherds were buried in several environmental contexts, recovered, and underwent absorbed pottery residue analysis. After two months of burial in three different soil types, plus a control stored in the freezer, caffeine was well-preserved in all sherds, suggesting that caffeine is a potentially useful biomarker for the detection of black drink.     

Indications of embalming in Roman Greece by physical,chemical and histological analysis

The partially mummified remains of a high-status female (ca. 1700 BP, Thessaloniki, Greece) were found inside a Roman-type marble sarcophagus containing a lead coffin. The individual was positioned on a wooden pallet, wrapped in bandages, and covered with a gold-embroidered purple silk cloth. Besides the clothes, remnants of soft tissue as well as the individual's original hair style and eyebrows were exceptionally well preserved. In addition to the macroscopic examination, microscopic and biochemical analyses were undertaken. Scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) analysis, and gas chromatography/mass spectrometry (GC/MS) were applied to examine the tissue preservation and probable mechanisms of mummification. The presence of chemical components, such as sesquiterpenes, triterpenoids, and diterpenoids, originating from coniferous and pistacia resins, myrrh, and other spices, verify ancient information on preparation methods of the dead in Greek and Roman times. These chemical components are thought to have played a prominent role in the mummification mechanism in this particular case. The potential effect of the lead coffin in the mummification process was also examined. Energy-dispersive X-ray analysis failed to detect lead penetration into the tissues, suggesting that the coffin played a limited role in the preservation of soft tissue.     

Essentials in the use of mycolic acid biomarkers for tuberculosis detection: response to “High-throughput mass spectrometric analysis of 1400-year-old mycolic acids as biomarkers for ancient tuberculosis infection” by

High molecular weight long-chain mycolic acids are key structural components of the cell envelope of Mycobacterium tuberculosis and they are established as biomarkers for the identification of both ancient and modern tuberculosis. Mycolic acids from M. tuberculosis have a characteristic profile, reflecting contributions from five major distinct homologous series of mycolate structural types. Diagnosis of tuberculosis in archaeological material, using mycolic acid biomarkers, depends on objective recognition of the key characteristic mycolic acid components. A recent article in this journal claimed that tuberculosis could be confirmed in ancient bones by high throughput mass spectrometric analysis of mycolic acids. Scrutiny of the data presented reveals no convincing evidence for the presence of mycolic acids, characteristic of the M. tuberculosis complex, in the skeletal remains examined. This communication reviews the essential criteria necessary for positive tuberculosis diagnosis, using mycolic acids.     

Starch grain characterization of Prosopis chilensis (Mol.) Stuntz and P. flexuosa DC,and the analysis of their archaeological remains in Andean South America

The fruit (pods) of Prosopis (Fabaceae) are frequently recovered from pre-Hispanic Argentinian archaeological sites, suggesting that this genus was of importance in ancient economies in this region. Yet it is only recently that archaeobotanists have begun to carry out systematic research into this genus. Therefore many questions remain to be addressed concerning the food value of Prosopis fruit, and its potential contribution. This paper examines starch from the pods of two species, Prosopis flexuosa and Prosopis chilensis, for the purposes of describing and classifying their morphological features and biometrical parameters. Pods of both species were gathered from two extremes (northern and southern) of the Hualfín Valley, Catamarca, Argentina. Starch abundance is estimated and compared with that of Zea mays, an economically important plant with high concentrations of starch. This paper reveals that Prosopis pods contain very low amounts of starch compared to starch-rich edible species. Despite this, the recovery of Prosopis starch grains can be successfully used to assess archaeological tool uses. Prosopis starch grains were found to be highly variable in shape. Grain size ranged between 10 and 20 μm. Granule irregularity and the high birefringence under polarized light are two of the most important diagnostic features. No significant statistical differences were found in the structure (morphology, size and hilum) of the starch of the same species from both localities. Finally, the implications for the role of this plant in past societies are discussed.     

Taphonomy of the bones of rodents consumed by Andean hog-nosed skunks (Conepatus chinga, Carnivora,Mephitidae) in central Argentina

The Andean hog-nosed skunks (Conepatus chinga) are generalized consumers. Rodents are a substantial part of their diet. In order to learn about the effects of this predator on the bones of the prey they consume, the bone remains from scats collected in La Pampa, Argentina, were analyzed from a taphonomic point of view. Analysis confirms that C. chinga produces severe destruction of bone remains, mainly during the chewing process. This allows inclusion of this predator in the category of extreme modifier of the bones of its prey. Data presented here and their interpretation provide criteria to identify the intervention of this predator in archaeological and paleontological sites.     

Identifying wine and oil production: analysis of residues from Roman and Late Antique plastered vats

The production of oil and wine was an important aspect of the ancient Mediterranean economy. Among the material remains related to the production of these substances, installations used for their production provide evidence that is still unexploited. Although archaeological studies have been carried out on these installations, problems can arise in the identification of their function, as wine and oil production structures can be similar and the archaeological traces insufficient to establish which substance was produced.     

Chemical characterisation of anthro-technosols from Bronze to Middle Age in Bologna (Italy)

The aim of this research was the understanding of a peculiarly expanded soil sequence in Bologna (i.e., Roman Bononia, Etruscan Felsina), one of the most important archaeological poles for Etruscan colonisation and settlement in northern Italy. The uppermost part of the chronological sequence dating from the Bronze Age up to medieval and modern times was analysed for the first time from geochemical and pedological viewpoints. The integration of archaeological, stratigraphical and pedological data supported by soil chemical analysis, a fundamental key for reconstructing a correct evolutionary sequence, made it possible to recognise seventeen buried Soil Units (ancient Technosols, Anthrosols and Cambisols) characterised by different kinds and degrees of intensity of anthropogenic influence. In some cases, the results show a relatively severe element concentration (Cu, Sn) mainly characterising the Iron Age (Etruscan and Villanovan) stratigraphic units, representing an ancient industrial pollution linked to local dispersal of melting activity dump products generated in near-site surrounding areas. Therefore, a comparison was possible between natural blank and anthropogenically polluted geochemical values for some local soils.     

Characterization of prehispanic cosmetics found in a burial of the ancient city of Teotihuacan (Mexico)

The present paper reports the chemical data obtained on samples of pigmenting materials contained in 31 miniature vessels found in a burial found in Teopancazco, a multiethnic neighborhood center located in the southeastern sector of the archaeological site of Teotihuacan (Central Mexico) and the analytical protocol established for the complete characterization of these archaeological materials. For this purpose a multi-technique approach based on the combination of several non destructive and micro-destructive instrumental techniques, namely, light microscopy (LM), scanning electron microscopy-X-ray microanalysis (SEM-EDX), transmission electron microscopy (TEM), voltammetry of microparticles (VMP), X-ray microdiffraction (μXRD), X-ray diffraction (XRD), UV-Vis spectrophotometry, FTIR spectroscopy and gas chromatography-mass spectrometry (GC-MS) has been developed in an attempt to completely characterize these materials. These instrumental techniques provide reliable and complementary data including elemental and mineralogical composition, identification of functional groups as well as speciation studies of electroactive species. Among the most relevant findings, volcanic pyroclastic rocks and clayey soils, most probably, from the Teotihuacan valley as well as galena, jarosite and mica from other provenances in Mesoamerica, have been identified. Charcoal and Ocote resin have also been identified. The chemical composition of the samples is discussed with a view to the possible function as cosmetic materials and their use in funerary rites. The results have been critically compared with those others obtained in other ancient civilizations. The study carried out on the materials found in the burial 105 is especially relevant insofar it represents the first evidence, scientifically characterized, of the use of cosmetics in Mesoamerica at this early period (AD 200-550).     

Environmental impacts around the time of Norse landnám in the Qorlortoq valley,Eastern Settlement,Greenland

Palynology, radiocarbon dating, and open-section stratigraphies from archaeological trenches are used to examine the impact of human activity around the time of Norse landnám on vegetation and landscape associated with a small farm (Ø34) in the Qorlortoq valley, Eastern Settlement, Greenland (61° N 45° W). Peat deposits from a mire abutting the Norse ruins revealed a discontinuous palaeoenvironmental record containing a possible hiatus from ca. AD 410–1020. Palaeovegetational data were recovered either side of this period. Pollen assemblages suggest that open Salix scrub dominated the landscape during the pre-settlement phase. The later phases of landnám resulted in the creation of hay fields and heavily-grazed grassy heath. Site abandonment is reflected by a re-expansion of Salix. This occurs shortly before the onset of deposition of a Sphagnum peat, dated to cal AD 1420–1630 (2σ) and reflecting an increase in mire surface wetness, probably in response to a deteriorating climate. Radiocarbon dates were obtained on peat and plant macrofossils sampled from either side of the proposed hiatus at two different but closely-spaced (<20 m) locations across the mire. These produced significantly different dates for the cessation of peat formation in the pre-landnám period (cal BC 2130–1770 and cal AD 240–410 respectively), but near-synchronous dates for the recommencement of peat growth (cal AD 890–1150 for peat and a probably more reliable interval of cal AD 1020–1190 based on plant macrofossils). It is suggested that this hiatus may represent the first direct evidence for peat cutting in Norse Greenland.     

Characterization of organic media in the wall-paintings of the “Palace of Nestor” at Pylos,Greece: evidence for a secco painting techniques in the Bronze Age

This paper presents the results of an investigation of organic binding media detected in samples from the Mycenaean wall-paintings at the “Palace of Nestor” in Pylos (Western Messenia, Greece): samples dated from the Late Bronze Age (ca. 1200 BC). This is the first scientific study to be conducted on organic binding media in Mycenanean painting, and it is a study that aims to contribute significantly towards our understanding of the original painting techniques used during the Bronze Age. The analytical procedures employed for the detection and characterization of the organic substances preserved within the paint layers (proteinaceous materials, lipids and plant gums), were based on chromatography/mass spectrometry techniques (PY–GC/MS, GC/MS). The most significant results, highlighting an a secco painting technique, are herein reported and discussed.