Fruits in foundation deposits of two temples

Foundation deposits underneath the walls of two temples near Semna, Sudan (c. 1500-1480 BC), included dried plant material. Five fruit species are represented: Vitis vinifera (grape), Ficus carica (fig), Citrullus vulgaris (water melon), Phoenix dactylifera (date) and Ziziphus spina-christi. Of particular interest are the remains of tubers of Cyperus esculentus. The plants mentioned above are known as funeral gifts in Egyptian tombs.     

Roman agriculture in the conventus Bracaraugustanus (NW Iberia)

A review of archaeobotanical data, together with new, still unpublished data, from Roman sites in conventus Bracaraugustanus suggests the Romanization of the northwest Iberia brought little changes to the agricultural strategies of local communities in indigenous-type settlements. The main crops remain the same as in the Iron Age: Triticum aestivum/durum, Panicum miliaceum, Hordeum vulgare subsp. vulgare, Triticum turgidum subsp. dicoccum and T. aestivum subsp. spelta, Avena and Vicia faba. The first secure reference for the cultivation of Secale cereale in the region comes from a Roman context, suggesting that it was introduced in this period, although it probably remained a minor crop.     

Sweet potato (Ipomoea batatas) and banana (Musa sp.) microfossils in deposits from the Kona Field System,Island of Hawaii

Analysis of plant microfossils (pollen, phytoliths, starch grains and xylem cells) was carried out on 12 soil samples from a variety of pre-contact archaeological features in South Kona within a portion of what has been termed the Kona Field System, on the Island of Hawaii. The oldest radiocarbon ages of the sampled deposits are 1300–1625 AD and 1310–1470 AD. The pollen and phytolith evidence suggests a change from more to less trees and shrubs in the area as a result of human activity. We found phytoliths of banana (Musa sp.) leaves in most and starch grains and xylem cells of tuberous roots of c.f. sweet potato (Ipomoea batatas) in all of the samples, suggesting that the sampled features were associated with these crops and that both crops were cultivated intensively within the study area. Higher concentrations (volumetric) of starch and xylem in samples from older deposits suggest that cultivation was more intensive then. The apparent absence of starch and xylem remains of other tuberous crops archaeologically identified elsewhere in Polynesia suggests that tuberous cropping within the study area was mono-specific.     

On the origin of the tree Spondias mombin in Africa

This paper examines evidence for the origin of the tree Spondias mombin in Africa, where it is generally described as introduced from the Neotropics. Based on biogeographical, ecological, and historical evidence, this paper argues that the tree reached Africa via natural, long-distance dispersal, and should be considered an African native plant. The idea that it is introduced appeared in 1868, when it was a scientifically justifiable conclusion reflecting the limitations of nineteenth-century biogeographical knowledge. By the early twentieth century, representing Spondias mombin as a human introduction in Africa was no longer scientifically justifiable because of advances in knowledge of the African flora and plant dispersal ecology. Nonetheless, most authors continued to represent the tree as introduced, in part because such representation was consonant with dominant images of the African environment: (a) that the continent had suffered extensive deforestation in the recent past, and (b) that Africa's flora was depauperate in fruit trees prior to the coming of Europeans. Recent authors continue to describe Spondias mombin as introduced because they have not sufficiently considered the historic contexts of primary and secondary sources on Neotropical plant introductions in Africa. This paper concludes that the human role in creating the African portion of the trans-Atlantic tropical flora has been overemphasized. Natural plant dispersal across the Atlantic may be more frequent than generally accepted.     

Ancient DNA analysis of desiccated wheat grains excavated from a Bronze Age cemetery in Xinjiang

Wheat has been one of the most important crop in Eurasia since the Neolithic period. Understanding the spread of wheat cultivation is crucial to understanding the spread of agriculture as a whole and the interactions between prehistoric populations across the Eurasian continent. However, the routes by which wheat cultivation spread eastwards have been poorly understood to date, due to the scarcity of plant remains recovered from archaeological sites. Desiccated wheat grains excavated from the Xiaohe cemetery in Xinjiang, and dated to the early Bronze Age, show excellent DNA preservation. Here we present an ancient DNA (aDNA) analysis of wheat (Triticum sp.) grains excavated from Xiaohe and provide the first definitive evidence for bread wheat in China during the Bronze Age. The nuclear ribosomal DNA internal transcribed spacer regions (ITS1 and ITS2) and the intergenic spacer region (IGS) were amplified. The IGS region within the D genome of wheat has a 71 bp insertion that is absent from corresponding regions in the A and B genomes. The results showed that the Xiaohe wheat showed most sequence similarity to hexaploid bread wheat (Triticum aestivum), including the characteristic insertion into the D genome. The presence of bread wheat at the Xiaohe cemetery is discussed in relation to it having spread into Xinjiang by the Bronze Age, providing new insight into the origins of bread wheat in East Asia.     

Early and mid Holocene tool-use and processing of taro (Colocasia esculenta), yam (Dioscorea sp.) and other plants at Kuk Swamp in the highlands of Papua New Guinea

Recent multidisciplinary investigations document an independent emergence of agriculture at Kuk Swamp in the highlands of Papua New Guinea. In this paper we report preliminary usewear analysis and details of prehistoric use of stone tools for processing starchy food and other plants at Kuk Swamp. Morphological diagnostics for starch granules are reported for two potentially significant economic species, taro (Colocasia esculenta) and yam (Dioscorea sp.), following comparisons between prehistoric and botanical reference specimens. Usewear and residue analyses of starch granules indicate that both these species were processed on the wetland margin during the early and mid Holocene. We argue that processing of taro and yam commences by at least 10,200 calibrated years before present (cal BP), although the taro and yam starch granules do not permit us to distinguish between wild or cultivated forms. From at least 6950 to 6440 cal BP the processing of taro, yam and other plants indicates that they are likely to have been integrated into cultivation practices on the wetland edge.     

Dietary analysis of Piraino 1, Sicily,Italy: the role of archaeopalynology in forensic science

Pollen from intestinal contents of mummies, backed by macrofloral analysis, provides important clues to diet, medicines, and season of death. Intestinal contents were recovered from the Piraino 1 mummy from the “Sepulcher of the Priests”, Piraino Mother Church, in the province of Messina, Sicily. Using standard palynological methods and pollen concentration technique, we quantified the numbers of pollen grains per gram of coprolite. The pollen spectrum was dominated by Polygalaceae, the Milkwort Family. Polygalaceae pollen is rarely found in archaeological sites. Based on comparison to published keys, we determined that the pollen came from a species of Polygala. Polygala contains species with medicinal value. We found nine other pollen types. Traces of Potamogeton (pondweed) was observed in preliminary scans. Multiple grains of Brassicaceae (mustard family), cereal (cultivated grains), Typha (cattail) and Cheno-am were found. Single grains of Castanea (chestnut), Fabaceae (bean family), Salix (willow), and Solanaceae (tomato family) were found. The preservation of the pollen was poor except for Polygala and the cereal pollen. Brassicaceae and Cheno-am have been part of the natural pollen spectrum in Sicily since ancient times. Cereal grains were consumed with prepared food. Importantly, background arboreal pollen was nearly absent. This indicates that Piraino 1 died during months of low pollination. The absence of olive pollen is important since this plant in Sicily reaches its maximum pollination in May and June and tapers off rapidly by mid-June. Therefore, absence of the key warm season airborne pollen type suggests a post-June death. Macrofossils, especially residue from grape pulp, indicates a death in September to November. His cause of death was likely a result of multiple myeloma. The methodological differences between archaeopalynology and forensic palynology are summarized. We suggest that the palynological methods presented here should be adopted for human remains analysis in forensic palynology.     

New insights into the consumption of maize and other food plants in the pre-Columbian Caribbean from starch grains trapped in human dental calculus

In a first region wide study, starch grains from human dental calculus from the pre-Columbian insular Caribbean (dating to ca. 350 B.C.–A.D. 1600) are used to identify important plant foods in the diet and to assess potential dietary differences related to age or sex. Results give important insights into pre-Columbian maize (Zea mays) consumption throughout the region, confirming recent studies that indicate that maize was more commonly consumed in the insular Caribbean than originally thought. No age or sex based differences in maize consumption were found. Furthermore, based on the results of new experiments regarding grinding and pressure damage to starch grains, it is clear that maize in the Caribbean was ground, baked and consumed as bread as was the case in large parts of the mainland. Based on our results we tentatively suggest maize consumption in the Caribbean was at least in some cases associated with feasting and ceremonial activities. The variety in other plant foods identified (mostly tuberous root crops) shows that the pre-Columbian inhabitants of the region consumed a broad spectrum, but locally variable diet in which a variety of root crops functioned as staple crops, including marunguey (Zamia sp.) and sweet potato (Ipomoea batatas). We found no indications for the traditionally assumed heavy reliance on manioc (Manihot esculenta) cultivation in the region.     

Plant microfossils in prehistoric archaeological deposits from Yuku rock shelter,Western Highlands,Papua New Guinea

Plant microfossil analysis was carried out on anthropogenic deposits from Yuku rock shelter, possibly occupied from before 14,200 cal. BP to recent times, in the Papua New Guinea Highlands. Elaeocarpus pollen has extremely high percentages throughout. Starch residues of lesser yam (Dioscorea esculenta) and one or more, undifferentiated Dioscorea species were identified, suggesting processing of these taxa since first use of the site. The latter include Dioscorea alata, Dioscorea bulbifera, Dioscorea nummularia and Dioscorea pentaphylla. Pollen and phytoliths of banana (Musa) appear 5200 cal. BP. We cannot differentiate between naturally growing and cultivated yams and bananas. Putative truffle (hypogeous Ascomycotina) spores in the deposits suggest foraging of truffles.     

Discovery of Beeswax as binding agent on a 6th-century BC Chinese Turquoise-inlaid Bronze sword

Recent archaeological field surveys and excavations at Qiaojiayuan, a platform 40 km west of the Yun County in present-day northwestern Hubei Province (central China), revealed four tombs (M₃, M₄, M₅ and M₆) dating back to around 6th to 5th century BC. The grave's layout, artifact assemblages and burial practice indicate that the owners of these four graves were aristocrats of the Chu state. Five turquoise-inlaid bronze artifacts (two swords and three dagger-axes) were excavated. The sword from M₄, imbedded with pieces of well-cut and finely-polished turquoise on its handle, was in a very bad state of preservation and underwent conservation treatment. Some sticky, whitish pastes were exposed when the inlaid turquoises dropped off. The pastes were supposed to be residues of unknown binding agents. Samples of the pastes were collected for FTIR (Fourier Transform Infrared Spectroscopy) and XRD (X-Ray Diffraction) analyses to determine the chemical component(s). The results revealed that the pastes were composed of almost pure beeswax. This indicated that beeswax was used as binding agent in turquoise-inlaid bronzes as early as the 6th to 5th century BC in China. Review of recent technical studies suggested that beeswax was used in early China in several different regions (mostly southern part of China) for at least two different purposes (binding agent and lightening).     

The first fossil crow (Corvus sp. indet.) from the Early Pleistocene Nihewan Paleolithic sites in North China

We describe a three dimensional preserved right tarsometatarsus, representing the first fossil crow from the well-known Early Pleistocene Nihewan paleolithic sites in North China. The new specimen is distinguished from other known species of the genus Corvus by the combined morphological features, especially the peculiar hypotarsus. The phylogenetic significance of the arrangement of canals at the proximal hypotarsus in passerines is discussed. Although the fossil was too incomplete to warrant the erection of a new species of Corvus, it nonetheless increases the taxonomical diversity of the Nihewan fauna and improves our knowledge on Pleistocene birds in China. Finally the paleoenvironment of the Nihewan sites are discussed in terms of its faunal composition.     

Agriculture and exploitation of wild plants at Chalcolithic (4th to 3rd millennium cal BC) sites in Sardinia (Italy)

This paper present the results of archaeobotanical analysis carried out at two open-air sites dated to the first phase of the Copper Age, “Chalcolithic” (4th to 3rd millennium cal BC) in Sardinia. The sediment was systematically floated, a total of 3142 l was sampled and 4014 charred plant remains were identified. Chalcolithic agriculture in Sardinia was primarily based on the cultivation of Hordeum vulgare, H. vulgare var. nudum and Triticum aestivum/durum. Possible cultivated legumes of Vicia/Lathyrus, Vicia/Pisum and cf. Pisum sativum were identified. Linum sp. was also present, which may have been cultivated and exploited in Sardinia by the Chalcolithic community. The diet of the Chalcolithic community was complemented by the consumption of edible fruits such as Ficus carica, Sambucus sp. and Vitis vinifera subsp. sylvestris. Typical Mediterranean shrubs such as Pistacia lentiscus, Juniperus sp., Cistus sp., Malva sp. and Thymelaea hirsuta were also found.     

Plant remains and amphorae from the Roman harbour under Flacius Street in Pula (Istria,Croatia)

The aim of this archaeobotanical and archaeological research is to expand knowledge about Roman diet and plant cultivation in Istria and to compare it with similar localities on the Eastern Adriatic coast. We have also tried to find some new information about maritime trade routes in the Mediterranean area in ancient times. Out of 27 samples collected from an excavated Roman port in Flacius Street in Pula, in total, 9809 plant macrofossils were recovered, identified and analysed. The results of the analysis show that most of the plant remains belong to the group of fruit trees and nuts. The most abundant are the remains of Ficus carica, Pinus pinea, Vitis vinifera subsp. vinifera, Rubus fruticosus agg. and Olea europaea subsp. europaea. These are all species that are widespread in the Mediterranean area and have likely always been consumed by the local population. The number of ruderal and weed species found is relatively high (31) in comparison with other plant categories (fruit trees and nuts; fruit collected from the wild; cereals; condiments; oil crops; vegetables and tubers; plants of fresh water environments; elements of maquis), but as they came to the site accidentally and not by targeted human activity, there are far fewer macrofossils of such plants than those of cultivated species. There were a few elements of evergreen forest vegetation and plants of aquatic habitats at the site (2 + 1), which suggests the existence of this type of vegetation in the area of the site in Roman times. Archaeobotanical comparisons of the site in Flacius Street with similar coastal Roman sites—Verige Bay on Veli Brijuni (first-fifth century ad), the port of Zaton near Nin (first-third century ad) and Caska Bay on the island of Pag (first and second century ad)—reveal considerable similarities, confirming the uniformity in nutrition and plant growth in the wider coastal area. Together with the two Roman ships, during the archaeological excavations of the Roman harbour and its layers, we collected over 2000 different archaeological artefacts out of which a large number was almost perfectly preserved. Some of the mentioned artefacts include ceramic amphorae, ceramic table- and kitchenware, ceramic lamps, different usable objects made of glass, wooden use objects, parts of ship’s equipment and other wooden tools, architecture elements from the nearby port as well as residential structures and remains of stone monuments. Because of the large amount of artefacts found at the site, the analysis of the artefacts and data processing are still in progress. As a contribution for recognizing organic remains, we isolated the amphorae whose purpose was the storage and maritime transportation of different food products and ingredients.     

The prehistoric pile-dwelling settlement of Stagno (Leghorn,Italy): wood and food resource exploitation

A pile-dwelling settlement, dated to the final Bronze–early Iron Age (XII–XI century BC), was excavated at Stagno, near Leghorn (Tuscany, Italy). The site presented a well-preserved portion of the wooden foundation structure buried by sediments rich in plant remains: both wood and plant remains were subjected to archaeobotanical investigation. The ultimate goal was to improve our knowledge on agricultural economy and wood usage in Tuscany during the prehistoric ages. The results pointed to a farming system based on Triticum spp. (wheat), Hordeum spp. (barley) and Leguminosae cultivation in addition to the gathering of wild fruits, such as Corylus avellana, Cornus mas, Prunus spp., Vitis spp. Many of these plant remains are associated to a wetland context. Mesohygrophilous trees, such as deciduous Quercus, Ulmus minor, Fraxinus cf. excelsior, and Sorbus were used for the construction of the pile-dwelling structures; the choice of these plants indicates a good knowledge of the technological characteristics of timber.     

In a nutshell: the identification and archaeological application of experimentally defined correlates of Macrozamia seed processing

This paper describes the methods and results of ethnographically-based replicative seed processing experiments conducted on Australian Macrozamia moorei seeds. Archaeological evidence indicates that these seeds were consumed by Australian hunter–gatherers in both the Pleistocene and Holocene. The experiments identified three features indicative of human processing and tested the effect of different processing variables (striking position, indentor type, thermal modification and presence of outer flesh) on the frequency of these diagnostic criteria. The results demonstrate that it is possible to use the physical modifications on individual seed specimens to identify the anthropogenic fracture of seeds and specific methods used to process them. These criteria were used in the analysis of M. moorei seeds from mid-Holocene-aged archaeological sites in central Queensland, Australia. The results indicate that seeds with and without sarcotesta were processed using a wooden indentor via side striking techniques.     

Archaeobotany,vine growing and wine producing in Roman Southern France: the site of Gasquinoy (Béziers,Hérault)

Unequivocal evidence of Roman vine cultivation and wine making is provided from studies of combined archaeological remains from the site of Gasquinoy (Southern France). Waterlogged and charred plant material (fruits/seeds/wood) collected from wells located in the close vicinity of cultivated fields and wine making establishments confirms the local significance of this activity. The results offer insights on particular aspects of wine production (‘traumatic’ treading of grapes and straining) and provide evidence of secondary agricultural activities such as cereal production and fruit tree cultivation. The potential use of monocotyledonous stems such as Arundo/Phragmites in the farming system is discussed.     

DNA analysis in charred grains of naked wheat from several archaeological sites in Spain

In the present work we attempt to recover endogenous ancient DNA from cereal grains preserved under different conditions: charred, partially charred and waterlogged. A total of 126 grains from naked wheat and 18 from barley from different sites on the Eastern Iberian Peninsula ranging from the beginning of agriculture in the region to the turn of the Common Era, were studied. Two different extraction protocols were used, a standard phenol–chloroform method and a silica-based DNA extraction procedure implemented for artificially charred seeds. Amplifications were directed to three markers: the large subunit of ribulose 1,5 biphosphate carboxylase (rbcL) and the microsatellite WCT12 in the chloroplast genome and the x and y subunits of the high molecular weight glutenin gene (Glu-1) in the nucleus. The first two were used to assess the preservation status of the samples, while with the third we tried to identify the wheat grains at species level. It was possible to obtain eleven positive amplifications in 8 partially charred seeds but only two amplifications of the Glu-1 gene from a single sample of the Early Bronze age were genome-specific. Different contamination sources were identified and reported. Cloning and alignment of sequenced clones showed a correspondence of the amplified fragment to modern wheat D genome haplotypes. This result suggests that the sample corresponds to hexaploid wheat (Triticum aestivum L.), thus being the first ancient DNA evidence to date for the cultivation of hexaploid wheat in the prehistoric agriculture of the Iberian Peninsula. Moreover, obtained results highlight contamination problems associated to the study of ancient archaeobotanical charred seeds suggest that the combination of a silica-based extraction method together with the amplification of specific targets is a good strategy for recovering endogenous ancient DNA from this kind of material.     

The origin of the ancient Egyptian drink Shedeh revealed using LC/MS/MS

Ancient Egyptians were buried with the most precious food and drink as sustenance for their afterlife. One of these was Shedeh, the most valued and appreciated beverage in ancient Egypt. The botanic origin of Shedeh remains unclear as no mention of its raw material has survived. Some scholars have proposed that Shedeh was a pomegranate wine, while others, a grape wine. Presented here is the first ever analytical evidence of Shedeh's origin through the analysis of a sample of a residue from an extraordinarily well preserved Shedeh amphora from King Tutankhamun's collection. The previously developed LC/MS/MS wine markers method for archaeological samples was used and our results reveal Shedeh had a red grape origin.     

Starch grain characterization of Prosopis chilensis (Mol.) Stuntz and P. flexuosa DC,and the analysis of their archaeological remains in Andean South America

The fruit (pods) of Prosopis (Fabaceae) are frequently recovered from pre-Hispanic Argentinian archaeological sites, suggesting that this genus was of importance in ancient economies in this region. Yet it is only recently that archaeobotanists have begun to carry out systematic research into this genus. Therefore many questions remain to be addressed concerning the food value of Prosopis fruit, and its potential contribution. This paper examines starch from the pods of two species, Prosopis flexuosa and Prosopis chilensis, for the purposes of describing and classifying their morphological features and biometrical parameters. Pods of both species were gathered from two extremes (northern and southern) of the Hualfín Valley, Catamarca, Argentina. Starch abundance is estimated and compared with that of Zea mays, an economically important plant with high concentrations of starch. This paper reveals that Prosopis pods contain very low amounts of starch compared to starch-rich edible species. Despite this, the recovery of Prosopis starch grains can be successfully used to assess archaeological tool uses. Prosopis starch grains were found to be highly variable in shape. Grain size ranged between 10 and 20 μm. Granule irregularity and the high birefringence under polarized light are two of the most important diagnostic features. No significant statistical differences were found in the structure (morphology, size and hilum) of the starch of the same species from both localities. Finally, the implications for the role of this plant in past societies are discussed.     

Plant microfossil analysis reveals disturbed forest and a mixed-crop,dryland production system at Te Niu,Easter Island

Plant microfossil analysis was carried out on 12 soil samples from a variety of landscape features at three locations (100–250 m a.s.l.) along a 950 m transect at Te Niu, Easter Island. Pollen and phytolith assemblages were dominated by palms and ground ferns, and suggested disturbed forest. We identified pollen of bottle gourd (Lagenaria siceraria) and starch grains of the common yam (Dioscorea alata), sweet potato (Ipomoea batatas) and taro (Colocasia esculenta). The data suggest a mixed-crop, dryland production system at Te Niu dominated by yam and sweet potato, and supplemented by taro and bottle gourd. The data provide direct evidence of crop type and range, supporting the indirect evidence (topographic and landscape features, field and historical research, comparisons with elsewhere in the Pacific) that much of the rock covered landscape of Easter Island was used for intensive horticulture.