‘Rid grasse of bones’: A taphonomic study of the bones from midden deposits at the Neolithic and Bronze age site of Runnymede,Surrey, England

Runnymede has large samples of Neolithic and Late Bronze Age animal bones, with contrasting preservation conditions in both periods. The bone evidence has been used to interpret the formation of the site deposits. There are few articulated bones, and no joins were found in butchered bone, indicating that the area studied did not contain primary refuse. Various aspects of bone alteration have been analysed: (i) the proportion of bones with very good surface preservation was high in the in situ Neolithic excavation units and the basal Bronze Age midden, but bones in the upper units were mostly eroded. These units are reworked flood deposits. The greater degree of fragmentation of the bone in the reworked units has been quantified, using a system of recording the ‘zones’ present on each bone, which allows calculation of the fraction present. It is also demonstrated that the reworked units contain a lower proportion of identified bones and a higher proportion of teeth and iaws than the units with well-preserved bone, (ii) Quantification of canid gnawing shows, unexpectedly, that more was recorded on well-preserved bone. Thus recognition of gnawing depends on bone condition. This also confirms that most of the erosion of the bone surface is a post-depositional phenomenon. The sequence of activities is therefore interpreted as follows: meat was cooked and consumed, and the bones discarded for the dogs. At a later stage, larger bones were picked up and thrown away in the river or midden. Some ethnographic examples of periodic cleaning of farming settlements are cited.     

Experimental effects of bone size and temperature on bone diagenesis

In the ground, bone undergoes chemical and physical changes which affect its preservation. This fact has important implications for dating and other analytical procedures involving bone, as well as faunal analysis where differential preservation of bones of different species may affect conclusions regarding the relative significance of an animal to the economy of a given society. The diagenic processes in bone range from minor changes in the bone protein to complete structural and chemical breakdown.Using fresh cow bone, we conducted laboratory experiments which simulate the effect of temperature and bone size on the rate and nature of bone disintegration in archaeological sites. Temperature influences the rate of chemical change, and bone size and density affect the accessibility of the molecular constituents of bone to extrinsic chemical reactions. These findings clarify the importance of two well-known concepts in bone taphonomy. (1) The rate of chemical breakdown in bone tissues is related to the proximity of a given unit of tissue to the bone surface. This means that, in archaeological bone samples, tissue near the surface may be different chemically from tissue away from the surface and great care is necessary in choosing and preparing bone samples for analytical procedures. (2) In general, small bones are not as well preserved as large bones, therefore small animals are likely to be underrepresented in faunal assemblages.     

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones.     

Pick the Right Pocket. Sub‐sampling of Bone Sections to Investigate Diagenesis and DNA Preservation

Many archaeological bones display a heterogeneous degradation pattern. Highly degraded bones could contain pockets of well‐preserved bone, harbouring good quality DNA. This dichotomy may explain why the relationships between global bone preservation parameters such as histological integrity, bone mineral crystallinity or collagen yield, and bulk DNA preservation/amplification success rate have been found to be at best, weak to moderate. In this pilot study, we explore whether or not a more localised approach will highlight a stronger relationship between diagenetic parameters and DNA preservation. This study includes a detailed histological characterisation of bone diagenesis in sub‐areas of three bone samples. Regions of the same bone, which displayed differential degrees of preservation or type of diagenesis were sampled for further analysis and both genetic (small scale Illumina MiSeq sequencing) and chemical (Fourier‐transform infrared spectrometric analysis) analyses were performed. The aim was to investigate how bone diagenetic processes relate to DNA preservation at a higher resolution than in previous studies. This is key in order to improve DNA analytical success rates. The expected relationship between bone and DNA preservation (retrieved endogenous DNA) was observed and the results corroborate previous work that DNA preservation is linked to the integrity of bone collagen and mineral. The results further suggest that non‐biological diagenetic alterations such as etching and the presence of mineral infiltrations and inclusions have a negative effect on DNA preservation/extraction. Copyright © 2016 John Wiley & Sons, Ltd.     

Diagenesis and survival of osteocalcin in archaeological bone

It has been demonstrated that the protein osteocalcin can survive in bone in the archaeological record, and postulated that it has the potential to survive over geological time periods. The precise mechanism for this longevity of survival is not yet fully understood, and has not been extensively studied in comparison to other diagenetic aspects of archaeological bone. We report a comparison between osteocalcin survival and the state of preservation of more than 60 bones from 14 archaeological sites. The amount of osteocalcin, assayed immunologically, was compared with diagenetic parameters that measure: the amount of ‘collagen’ in the bone, the mineral changes, the porosity, and the histological preservation of the material. The findings indicate that microbial taphonomy and mineral alteration of bone have a profoundly damaging effect on the preservation of osteocalcin.     

Implications of heat-induced changes in bone on the interpretation of funerary behaviour and practice

Bones submitted to heat experience structural and chromatic modifications. In particular, heat-induced bone warping and thumbnail fractures have been linked to the burning of fleshed and green bones – where the soft tissues have been removed from the bones soon after death – in contrast to dry bones. Those have been suggested as indicators of the state of the individual before being burned thus allowing inferences about the funerary behaviour of archaeological populations. A large sample of 61 skeletons submitted to cremation has been examined for the presence of both of these heat-induced features. Although uncommon, bone warping and thumbnail fractures were present in some of the skeletons demonstrating that its presence is not restricted to the burning of non-dried bones as generally believed. Rather than being an indicator of the presence of bones with soft tissues, bone warping seems to be more of an indicator of the preservation of collagen–apatite links which can be maintained on dry bones with low collagen deterioration. In addition, our results also do not confirm thumbnail fractures as an exclusive sign of the burning of bones with soft tissues. As a result, these heat-induced changes should be used with caution when trying to infer about the pre-burning state of an individual.     

Túnel: A Case Study of Avian Zooarchaeology and Taphonomy

Although not often considered, there are many osteological characters unique to the avian skeleton that influence the taphonomy of bird bones. These characters are reviewed and their archaeological significance discussed herein. The presence of marrow in many avian long bones is important to interpretation of avian remains from archaeological sites because the presence of marrow affects bone density and, in turn, preservation. Other structural properties that affect avian bone preservation include cortical wall thickness, length and pneumatic state. Based on an analysis of approximately 10,000 bird bones from the archaeological site of Túnel, Tierra del Fuego, Argentina, I found that specific breakage patterns resulted from natural taphonomic processes acting as a result of the unique avian bone characteristics. This information may allow researchers to distinguish breakage patterns in avian bones resulting from natural taphonomic processes from breakage patterns that are culturally induced.     

Bone diagenesis in the European Holocene II: taphonomic and environmental considerations

We have applied cluster analysis to mercury intrusion porosimetry data from 219 archaeological bones (121 human and 98 animal) and soil chemistry data from 219 accompanying soil samples (1 per bone sample), to investigate the influence of soil chemistry on bone preservation. The samples chosen for the study were obtained from sites ranging in time from the pre-modern to the Mesolithic and were representative of burial environments across Europe (from the Baltic to the Mediterranean). These results represent the single largest database for archaeological bone preservation in the European Holocene to date and demonstrate the potential for large-scale diagenetic studies to help develop long term preservation strategies for our European heritage. Despite the variety of sites and environments, bones could be categorised into only four main diagenetic types. Furthermore, soil chemistry appears to significantly affect only one type of preservation, the pathway characterised by loss of mineral. In neutral to basic soils, taphonomy and in particular the differences between the treatment of human and animal remains, becomes the dominating factor in determining preservation. Using these results, strategies for heritage management of archaeological sites can be suggested; grouping sites into those requiring immediate excavation and those where in situ preservation is viable.     

湿度对骨质文物的影响及最佳存放湿度的研究

系统考察了在不同湿度下,老化400天后骨样的抗弯强度、色差值、显微形态、元素含量、尺寸和重量各项指标的变化,找出了湿度对骨质物影响的规律,从而确定60％湿度为骨质物的最佳存放湿度。     

Bone preservation and DNA amplification

The use of ancient DNA has increased during the past two decades in several scientific disciplines. However, the underlying mechanisms of DNA degradation in bone tissue are poorly understood. Here we address the importance of hydroxyapatite and collagen for DNA preservation in bone. We used two series of bones and teeth, one set of modern experimentally degraded bovid bones and one set of ancient horse bones/teeth. From these samples, we measured crystallinity, DNA presence and extracted collagen. The mtDNA fragments, parts of cytochrome b and the D–loop were amplified and sequenced. Our results show that presence of DNA was strongly related to the crystallinity in the hydroxyapatite and to the amount of collagen. This suggests that the hypothesis that hydroxyapatite has a crucial role in DNA preservation in calcified tissue is valid; and hydroxyapatite and collagen can be used to indicate whether DNA is present in the material. This is what would be expected if DNA is adsorbed to and stabilized by hydroxyapatite in calcified tissue, and collagen is part of the complex system that preserves DNA in bone tissue. Further, since collagen is the preferred material for radiocarbon dating, such bones may be a starting–point for a DNA analysis.     

Pre-screening techniques for identification of samples suitable for radiocarbon dating of poorly preserved bones

Under certain environmental conditions, post-depositional diagenetic loss of bone collagen can severely reduce the number of bones from a particular archaeological site that are suitable for stable isotopic analysis or radiocarbon dating. This study examined nearly 300 bones from 12 archaeological sites across southern England known to yield poor or variable preservation to try to identify one, or more, pre-screening technique(s) that would indicate suitable collagen preservation for radiocarbon dating. The most reliable method was shown to be the percent nitrogen (%N) of whole bone powder, which has an 84% chance of successfully predicting whether or not a bone will yield sufficient (i.e. >1% weight) collagen for dating.     

Patterns of Diagenesis in Bone II: Effects of Acetic Acid Treatment and the Removal of Diagenetic CO3

Archaeological bones of varying preservation have been treated with 0·1 M acetic acid in order to investigate the effect on structural and chemical alterations caused by diagenesis. Acetic acid is commonly used as a “cleaning agent” for removing diagenetic carbonate from bone and enamel, in an attempt to recover original, biogenic signals for use in dietary and¹⁴C dating studies.Diagenetic parameters were measured before and after treatment on a range of archaeological bones with good and bad preservation. Histological preservation defined the behaviour of the correlating parameters, where correlation coefficients between carbonate content and crystallinity, microporosity and macroporosity increased significantly after treatment. For histologically well preserved material, acetic acid is effective at returning carbonate content to around that of modern bone. Where bone is extensively damaged by micro-organisms, “loose” diagenetic material can be removed, but a fraction largely composed of hypermineralized bioapatite remains, which, we believe, cannot be reliably used to obtain accurate biological signals.     

The strange case of Apigliano: early ‘fossilization’ of medieval bone in southern Italy

The preservation of the osteological material at the medieval site of Apigliano, in southern Italy, is characterized by bones with highly crystalline, and altered, mineral phases. In addition to this, some material retains perfect histological preservation, with the exception of small microfissures present throughout this structure. Diagenetic porosity is indicative of collagen loss via chemical degradation. The levels of residual collagen in these bones are much lower than is predicted from simple models of gelatinization, and thus a more complex explanation for the state of preservation must be sought. Possible explanations for the rapid loss of bone collagen are considered, including a high–temperature event, the acceleration of hydrolysis due to liming and extreme wetting and drying cycles.     

In situ Preservation Solutions for Deposited Iron Age Human Bones in Alken Enge,Denmark

The rich mass deposition of Iron Age human bone material from the Danish site, Alken Enge, is extraordinary not only from an archaeological perspective but also from a preservation point of view. The main find is situated in a waterlogged, anoxic environment which provides excellent preservation conditions and therefore enables in situ preservation of the human bones. However, major differences in local environmental conditions challenge an in situ preservation of the entire site area as parts of the bone material presently deteriorate. In this paper, a multi-proxy environmental monitoring approach is used to document threats and to suggest the best preservation solution for the archaeological finds, whether in or ex situ.     

Bone Preservation and Ancient DNA: The Application of Screening Methods for Predicting DNA Survival

Given the technical difficulties associated with ancient DNA research, any methods that help to identify samples that will yield amplifiable DNA will be of great value. This study examined the relationships between gross preservation, histological preservation, bone size and the ability to amplify short fragments of mitochondrial DNA in 323 goose humeri from the Anglo-Saxon site at Flixborough. Bone size was not a good predictor of the presence of amplifiable DNA, but there was a significant association between both gross and histological preservation and DNA survival. This suggests that it is worthwhile to preferentially select morphologically well-preserved bones for ancient DNA studies. Our results with ancient avian bone mirror those previously obtained with mammalian archaeological bone, although the relationship between DNA survival and histological preservation was stronger for the latter.     

Quantifying histological changes in archaeological bones using BSE–SEM image analysis

Recent years have seen rapid developments in the understanding of diagenetic changes to archaeological bones. In particular, the degradation or preservation of proteins and other biomolecules has been explored using an increasingly sophisticated battery of analytical techniques. Problems remain, however, in correlating these parameters with physical changes to bone that may be observed microscopically. This is due, in part, to the problems in reproducibly quantifying histological changes to archaeological bone. This paper introduces a novel method for the accurate quantification of these changes employing image analysis of SEM images. Self–consistency of results was tested using measurements of total calcium content at different magnifications. The term ‘bioerosion index’ is suggested for the measured parameter.     

骨角质文物保护研究进展

骨角质文物丰富的历史价值使其成为世界各地考古学、人类学、艺术史研究的重要材料,但由于骨角质文物一般年代久远、保存环境各异以及自然矿化等因素造成该类文物的保存状况不容乐观。该文拟从骨角质文物保护研究的角度,对目前骨角质文物的制作材料与工艺、病害研究、修复技术及预防性保护等方面进行梳理,并尝试找出目前存在的问题与保护需求。我国骨角质文物保护需求主要包括出土饱水器物的现场保护是骨角质文物保护中亟待解决的问题;中国最早的古文字资料即有字甲骨的防风化研究是骨角质文物保护中的重点课题;全国各地出土的不同时期动物骨骼的保护是骨角质文物保护工作的主要内容。     

Well preserved non‐collagenous extracellular matrix proteins in ancient human bone and teeth

Ancient bones in a good preservation state, ascertained by microscopic techniques, conserve extracellular matrix proteins over thousands of years. With new techniques, intact extracellular matrix proteins from ancient bones and teeth are extracted and separated by one‐dimensional and two‐dimensional electrophoresis. Proteins were identified in Western blots by special antibodies against different human extracellular matrix (ECM) molecules of bone. We have confirmed different types of ECM human bone molecules such as osteonectin, osteopontin, and alkaline phosphatase with specific antibodies in human bone samples from different age groups. Additionally, we selected bone samples from different cultural time periods, such as the Middle Ages, the Bronze Age and the Late Pre‐Pottery Neolithic Phase (PPNB), and teeth from individuals from the Early Middle Ages and from the Late PPNB. The survival of intact extracellular matrix proteins in ancient bones and teeth dating from recent times to the Late PPNB, and reliable techniques to identify these proteins, present a great challenge to further research. A Match Set with PD‐Quest 7.2 shows that only 16% of protein spots in the teeth are also found in the bone of the same individual. In combination with the results of macro‐ and microscopic investigation, biochemical techniques will help us in obtaining a better understanding of bone and teeth in health and disease. Copyright © 2006 John Wiley & Sons, Ltd.     

Chronic Maxillary Sinusitis in Medieval Sigtuna,Sweden: A Study of Sinus Health and Effects on Bone Preservation

Frequencies of maxillary sinusitis in the population of the medieval town Sigtuna, Sweden, were examined. The first aim was to explore the occurrence of sinus conditions in this urban population, and the second was to investigate the effects of preservation in relation to these changes. The skeletal sample consisted of 274 adolescent and adult individuals buried ca 970–1530 AD. The maxillary sinuses were examined for different types of skeletal change related to sinusitis. The severity and location of these changes were recorded, together with the preservation of the sinus. When the diagnostic criteria for bone preservation from previous studies was used, i.e. the preservation of at least one complete antral floor, the results showed that 97.7% (n = 157) of the individuals were affected, with older individuals showing more severe bone changes. In the entire (i.e. more fragmented) sample, 94.5% (n = 259) showed signs of sinusitis. Again, older individuals showed more severe bone changes, and males were more frequently affected. Also, an increased frequency over time was found. The maxillae were subdivided into groups of bone preservation, and the results showed that there was a significant relation between preservation and the registered presence and severity of sinusitis. The results may be used as a cautionary note against investigating the occurrence of maxillary sinusitis without considering the skeletal preservation factor. There was no correlation between preservation and sex or age groups. This suggests that the differences between younger and older and male and female were not a result of preservation alone and could be considered as trustworthy. Copyright © 2011 John Wiley & Sons, Ltd.     

Bone Degradation and Environment: Understanding,Assessing and Conducting Archaeological Experiments Using Modern Animal Bones

Archaeological experiments that use modern bones to replicate past animal bone assemblages have often failed to consider the effects of environment, storage and preparation on modern bones. Often, these experiments make little mention of the conditions to which bones were subject during their storage and preparation for use in experiments. In other instances, these variables are reported but not considered as factors that contribute to the nature of the results obtained. This study considers previously reported data concerning the degradation of frozen bones (−20°C), and bones exposed to hot, dry conditions (40°C), and presents new data for bones exposed to room temperature environments (22°C) and refrigerated environments (2°C), and bones that are frozen (−20°C) and then thawed (22°C). These conditions are all relevant to understanding the nature of bone degradation and the use of bones in modern archaeological experimentation. This article also surveys a range of previously reported experiments that utilise modern bones to create analogies to the past and considers different methodological approaches and their relationship to the condition of bones at the time of their fracture and fragmentation. The longitudinal data presented in this study demonstrate differential rates of bone degradation over time in various environmental conditions. This degradation results in dramatic changes in bone fracture morphology, bone strength and utility for bone tool production. These observations have significant implications for experiments that utilise modern bones, especially when experimental data are used to create analogies to the archaeological past. Copyright © 2012 John Wiley & Sons, Ltd.