Biological Homogeneity and Familial Segregation in the Iron Age Population of Alfedena (Abruzzo,Italy), Based on Cranial Discrete Traits Analysis

The Iron Age necropolis of Alfedena has been well-known since the last century. In this study 10 non-continuous traits of the cranial skeleton are examined in a series of 94 adult individuals (54 males and 40 females). The division of the Alfedena necropolis into tomb circles has permitted the investigation of the possible existence of familial segregation. Our analysis has shown us that the individuals inhumated in the different tomb circles have a common genetic substrate probably attributable to the strong endogamy present in this population.     

DNA in cremated bones from an early bronze age cemetery cairn

The discovery of polymeric DNA in charred wheat grains and other burnt plant remains prompted us to examine a set of cremated bones for the presence of ancient DNA. Extracts of cremated bones from an early Bronze Age cemetery cairn were analysed by hybridization probing and the polymerase chain reaction. Hybridization with two probes targeting human DNA resulted in strong positive signals. Polymerase chain reactions directed at a multicopy sequence in human nuclear DNA were successful, extracts of five burials giving amplification products of the expected size. Exploitation of ancient DNA in cremated bones could be of great value in archaeology as it may enable information pertaining to sex and kinship to be obtained from fragmented material.     

‘Old wood’ effect in radiocarbon dating of prehistoric cremated bones?

Numerous reports of successful radiocarbon dating of cremated bones have emerged during the last decade. The success of radiocarbon dating cremated bones depends on the temperature during burning and the degree of recrystallisation of the inorganic bone matrix. During cremation bones undergo major morphological and mineralogical changes which have raised some interesting questions and discussion on the origin of the carbon source in archaeologically cremated bones. Recent laboratory experiments reveal that the properties of the combustion atmosphere play a significant role regarding the source carbon in cremated bones. Thus radiocarbon dating cremated bones is potentially dating the wood used for the cremation fire. Here we compare a high precision radiocarbon dated human bone with an associated dendrochronological age from an oak coffin. We find that the age discrepancy between the dendrochronological age and the cremated bone of 73 ± 26 ¹⁴C yr is best accounted for by the so called ‘old wood’ effect.     

Biological mineral content in Iberian skeletal cremains for control of diagenetic factors employing multivariate statistics

The aim of this study was to define a strategy for a correct selection of bone samples by employing inductively coupled plasma optical emission spectroscopy (ICP-OES) for reconstructing the biological mineral content in bones through the determination of major elements, trace elements and Rare Earth Elements (REE, lanthanides) in skeletal cremains of ancient Iberians (III–II B.C), discovered in the Necropolis of Corral de Saus (Moixent, Valencia) between 1972 and 1979. The biological mineral content was determined taking into account diagenetic factors. A control method for a better reading of results was applied. To explore large geochemical datasets and to reduce the number of variables, Principal Component Analysis (PCA) was used, thus, providing a deeper insight into the structure of the variance of the dataset. PCA shows that the elemental profiles of bone and soil samples are clearly different. Bone samples obtained from the outer bone layer were shown to have a different elemental composition; more similar to soil samples than samples of the inner bone layer. PCA scores and loadings plots were preferred to dendrograms obtained using Cluster Analysis, due to the limits of the latter one to appreciate the spatial ordering of samples. Partial least squares discriminant analysis (PLS-DA), a frequently used supervised classification method, was applied to differentiate between degradation states of bone samples. PLS-DA results obtained in this study confirmed that changes derived from different burning conditions were associated with transformations in the mineral part of the bones. Accordingly, carbonized bones can be differentiated from cremated bones. Class assignment of bone samples with uncertain thermal conditions in dependence on their elemental composition has shown to be feasible. Consequently, for biochemical-archaeological studies the analysis and statistical classification of carbonized and cremated archaeological bones, as well as those exposed to unknown thermal conditions together with experiments in modern bones, are recommended.     

A study by thermal treatment and X-ray powder diffraction on burnt fragmented bones from tombs II,IV and IX belonging to the hypogeic necropolis of “Sa Figu” near Ittiri,Sassari (Sardinia,Italy)

In the course of an anthropological investigation of three tombs of the hypogeic necropolis of “Sa Figu” (near the village of Ittiri in North Sardinia, Italy), numerous burnt bone fragments were collected. In particular, from the whole necropolis we selected a tenth of long bones from tomb IV, a small fragment of femur from tomb II and four fragments belonging to tomb IX. The aim of this work is to determine a temperature range to which the bone fragments were heated, in order to assess a funerary cremation which was presumably used at that time to quickly and hygienically eliminate the flashy parts of deceased people rather than a purification rite with fires inside the sepulchres. We attempted to evaluate the range of temperature to which the bones were likely subjected, making a joint use of thermal treatment and powder X-ray diffraction investigations. From our X-ray line broadening results carried out with a modern approach (Rietveld method), it emerged that a group of five fragmented burnt bones (one specimen belonging to tomb IV, the other four from tomb IX) were subjected to a heat treatment in a temperature range from 600 and 750 °C. Conversely another group of bones (belonging to tombs II and IV) turned out to be subjected to temperatures not higher than 250 °C.     

Characterisation and blind testing of radiocarbon dating of cremated bone

The success of radiocarbon dating of burned or cremated bones depends on the exposed temperature during burning and the degree of re-crystallisation of the inorganic bone matrix. We present a method for characterisation of likely cremated bones by employing visual inspection, infrared spectrometry and carbon stable isotope analysis on the bio-apatite fraction. The method of radiocarbon dating of cremated bones was tested by dating paired samples of bone and associated context materials such as pitch, charcoal and a dendrochronologically dated oak coffin. The dating of these paired test samples were largely performed as blind tests and showed excellent agreement between pitch and bone. The weighted mean age difference of all test samples is observed to −9 ± 60 ¹⁴C yr. To test the indicators and the effects of the degree of burning, a Late-Neolithic human individual has been studied, as this individual exhibits the full spectrum from low temperature burning (charred) to high temperature (“cremated”) from one end of a single bone to the other. This is reflected as a marked step in numerous parameters as well as in a significant difference in ¹⁴C age between the charred and the cremated bone samples.     

THE IRON AGE URNFIELD TRADITION OF SOUTHWESTERN JUTLAND,DENMARK

This study examines two urnfields, their development, burial rituals, grave goods and the cremated remains in a renewed analysis of the Danish Urnfield Tradition. The osteological investigation reveals a very high proportion of children's graves in these communal burial sites. Individual expression and demonstration of status are muted in burial rituals adhering to strict norms, although differences between age categories show through variations in the size of a burial monument. The use of CT scans and a detailed analysis of all artefacts provide evidence of the ritualized breaking of urns and the retrieval of bones from graves. Such retrieval of bones together with the layout and development of urnfields demonstrate the importance placed on the ancestors in the Early Pre-Roman Iron Age. The inconspicuous burials, together with the incorporation of all age categories, suggest that the focus of these burial communities is on a relational rather than individual identity.     

A Unique Case of Prone Position in the Primary Cremation Tomb 252 of Monte Sirai Necropolis (Carbonia,Sardinia, Italy)

A series of graves belonging to the Phoenician period were investigated after the campaign excavation of 2007 in the Phoenician‐Punic necropolis of Monte Sirai (Carbonia, Sardinia, Italy). One tomb in particular (labelled 252) is presented here because of its uniqueness. Tomb 252 contained the cremated remains of an individual, probably male, and comparison of the excavation records alongside reconstruction of the bone material itself makes clear that the individual was cremated in a prone position. It represents the first case of prone cremation reported in the literature. To investigate whether the temperature of the cremation was homogenous across the entire body, we have conducted a combined physical–chemical analysis using X‐ray diffraction spectroscopy and Fourier transform infrared. Copyright © 2012 John Wiley & Sons, Ltd.     

Skeletal analysis and mortuary practice in an Early Roman chamber tomb at Kenchreai,Greece

Recent exploration of the site of Kenchreai, the eastern port of Corinth in southern Greece, has focussed on a cemetery of subterranean chamber tombs dating chiefly to the Early Roman period (middle‐late 1st to 3rd centuries AD). The copious but fragmentary human bones and teeth found in Tomb 10 have been disturbed since burial by natural processes, including bedrock erosion and the infiltration of moisture, roots and basic sediment, and by destructive looters. Nonetheless, the remains furnish considerable information about the mortuary practices of wealthy residents. Analysis of the remains in their archaeological and taphonomic contexts reveals that Tomb 10 contained at least 23 individuals, 15 inhumed in loculi and 8 cremated and placed in niches, sometimes in urns. The identification of males, females and subadults among the burned and unburned bones and teeth suggests that spouses, parents and children probably from several generations of the same lineage or household were buried here. The nature of the cremated fragments reflects a laborious process during which mourners burned bodies on substantial pyres at extremely high temperatures for a long time. Then they carefully extracted a representative sample of small fragments from throughout the reduced skeleton for burial at the tomb. This study contributes to a better understanding of mortuary practices in the Roman East, particularly the Greek world, where the chamber tomb was a common sepulchral type. Copyright © 2009 John Wiley & Sons, Ltd.     

The Protohistoric ‘Quicklime Burials’ from the Balearic Islands: Cremation or Inhumation

Traditionally, the Balearic so‐called ‘quicklime burials’ of the Iron Age have been considered to be inhumations in quicklime. The general appearance of the bones, however, resembles more closely that of cremated bones. Laboratory tests reveal that the observed features of the bones from these burials, including cracks, thumbnail fractures and warping, cannot be explained by an inhumation in quicklime. The δ ¹³C value, Fourier transform infrared spectra, SF values and the low carbon content of the apatite moreover indicate a thermal manipulation of the bones. The ¹⁴C content is depleted with regard to the accepted archaeological age of the sample, which can best be explained by carbon exchange between bio‐apatite and fossil CO₂ released during the heating of limestone. This implies that the Balearic ‘quicklime burials’ must be interpreted as an elaborate cremation practice in presence of limestone. Copyright © 2013 John Wiley & Sons, Ltd.     

Distinguishing Remains of Human Cremations from Burned Animal Bones

Abstract

Archaeological remains of human cremations, often consisting of a few calcined bone fragments unassociated with any other indicators of human mortuary behavior, are sometimes difficult to distinguish from mammalian remains representing ancient garbage. Although experimental and archaeological studies of human cremation may facilitate recognition of cremated or otherwise burned mammal bone, they do not provide the necessary criteria to distinguish cremation from other kinds of burning and, therefore, to recognize, immediately, a human cremation in archaeological context. Experiments were conducted to provide criteria for distinguishing the remains of cremated whole large mammals from intensively burned fresh, anhydrous, archaeological, boiled, and baked bones of large mammals. Results indicate that distinctive patterns of cracking, fracturing, and warping of calcined bone, and especially the absence of evidence of perimortem fracture, may alert the excavator to the possibility of a human cremation.     

A new calibration of the XRD technique for the study of archaeological burned human remains

A new calibration of human bones as a function of programmed temperature (200–1000 °C) and time (0, 18 and 60 min) is presented and discussed in order to investigate the issues related to the study of cremated bone remains by using the powder X-ray diffraction approach. The experimental results confirm the growth of hydroxylapatite crystallites as a function of the applied temperature, with a sigmoid behaviour that has been parameterized to the experimental data points. Particularly, it was observed that the thermal treatments for 60 min anticipate of about 100 °C the effects that are otherwise observed after the treatments for 0 min. The developed procedure was subsequently applied to cremated remains of various archaeological sites of Spain and supplied precise information not only about the temperature reached during the funerary rites, but also on the presumed duration for the cremation.     

Anthropological and physicochemical investigation of the burnt remains of Tomb IX in the ‘Sa Figu’ hypogeal necropolis (Sassari,Italy) – Early Bronze Age

Excavations carried out in Tomb IX of the hypogeic necropolis of ‘Sa Figu’, near the village of Ittiri (Sassari, Italy), supplied burnt human bone remains and pottery unambiguously referred to the Early Bronze Age (characterised by the local culture of ‘Bonnannaro’). Besides the anthropological study, we have investigated and evaluated the possibility of a funerary cremation practice in Sardinian pre‐history, a subject that has previously not been considered from a scientific point of view. Making use of a calibration procedure based on X‐ray diffraction (XRD) line‐broadening analysis, related to the microstructural properties, it was possible to estimate the combustion temperature to which the fragmented bones were subjected. It was found that the studied bones reached temperatures varying from 400°C up to a maximum of 850°C. This spread of values suggested inhomogeneous combustion of the bones, which seems compatible with funerary cremation practices. Copyright © 2007 John Wiley & Sons, Ltd.     

Fluvial dispersal potential of guanaco bones (Lama guanicoe) under controlled experimental conditions: the influence of age classes to the hydrodynamic behavior

Hydrodynamic sorting is a taphonomic process able to transport and scatter bones deposited in archaeological and paleontological sites. This study presents the results of experimentation performed in an artificial flume with guanaco (Lama guanicoe) bones of different ontogenetic development, dry and saturated in water, in hydric flows velocities of 15 and 30 cm/s. The obtained results show that bone global density, the age of the individual, the dry or wet bone state, and the hydric flow velocity influence significantly bone dispersion. In this way, bones from immature individuals with unfused secondary growth centers and relatively low bulk density have better possibility of being transported than fused bones from adult individuals. Taking into account the results obtained in this experimentation and the feasibility of discriminating age categories in fossil assemblages, two bone groups with differential potential transport are presented in this paper. These transport groups constitute a methodological tool to evaluate the role hydric current may had played in the formation of a fossil assemblage.     

Morphometric Variability of Roman Dogs in Hispania Tarraconensis: The Case Study of the Vila de Madrid Necropolis

The size, body conformation and skull shape of Roman dogs from the ‘Vila de Madrid necropolis’ site, where 1480 dog remains were recovered, are described in this paper to provide evidence of the morphometric variability of Roman dogs in Hispania Tarraconensis. The individualized and detailed morphometric analysis of 10 individuals shows that the proliferation and explosion of the morphological variability already documented in different regions of the Roman Empire (Italia, Gallia, Britannia, Pannonia) are also noted here. The presence of very different individuals in terms of their shape and size, similar to the differences that can at present be noted between Pekingese and Doberman dogs, has been documented at the site. The reasons for the appearance of this morphological variability within those territories that were part of the Roman Empire are also discussed. Copyright © 2015 John Wiley & Sons, Ltd.     

Tempo and mode of formation of the Late Epigravettian necropolis of Arene Candide cave (Italy): direct radiocarbon evidence

Existing ¹⁴C dates of charcoal and bone samples from different layers of the Late Epigravettian sequence at Arene Candide (Liguria, Italy) place the formation of the deposit between 12,000 and 10,000 (uncal.) BP, but it is unclear how long this cave was used for funerary purposes. Clarifying this point has important archaeological and anthropological implications, given number and variety of inhumations. These include single and double burials of richly ornamented adults, adolescents and children and disarticulated accumulations of bones. Six new direct ¹⁴C AMS dates obtained from human skeletons place more precisely the necropolis at the Late Pleistocene-Early Holocene boundary, and appear distributed in two distinct groups that indicate two phases of funerary utilisation of the cave. Although separated by many centuries, the two phases of interment show recurring formal elements. Both groups include skeletons in anatomical connection exhibiting similar grave goods as well as chaotic accumulations of bones and, more importantly, the double burial of an adult with a child lying to its left. The reappearance of similar funerary patterns in the same site at two widely separated points in time suggests that the cave held a symbolic significance over several centuries. This, and the additional evidence of funerary use of the cave during the preceding Gravettian, points to the endurance of Upper Palaeolithic cultural traditions.     

Is X-ray diffraction able to distinguish between animal and human bones?

The possibility of determining the human or animal origin of bones from the lattice parameters of their inorganic bioapatite phase, when subjected to a high temperature treatment using the powder X-ray diffraction (XRD) technique, has been explored on a wide number of specimens. Forty-two animal bones were treated in a furnace at 1100 °C for 36 min and compared to 53 cremated human bones from a range of ancient necropolises. The X-ray diffraction patterns of bioapatite were simulated using both monoclinic P21/b and hexagonal P63/m structures to verify any occurrence of phase transformation and any difference in the lattice parameters due to the model. It was determined that the differences between the a-axis and c-axis of the monoclinic and hexagonal lattice were unimportant. Some outlying values were revealed to be caused by the presence of chlorine ions diffused into the apatite structure increasing its average unit cell values. Nevertheless, our results clearly show that in terms of lattice parameters the variability of human specimens are completely overlapped by the non-human variability making the use of XRD in order to distinguish animal from human bones questionable.     

Synchrotron radiation microprobe analysis of human dental calculi from an archaeological site: A new possible perspective in palaeonutrition studies

The X-ray microprobe at beamline X-26 of the National Synchrotron Light Source (Brookhaven National Laboratory) has been used to determine minor and trace elements in small fragments of dental calculus. This material was removed from 11 individuals found in the Norman necropolis discovered recently under the church of San Lorenzo di Aversa (Caserta, southern Italy, ca. AD 900–1200). The aim of these experiments is to develop new methods for non-destructive analysis of organic archaeological materials in studies of palaeonutrition and palaeopathology. These preliminary analyses show little variability in the main trace elements of dietary significance, such as Sr and Zn.     

In sickness or in health? Assessment of Early Iron Age human skeletons from Toutswe sites,east central Botswana

The general health of Early Iron Age (AD 700 to 1300) mixed farmers in east central Botswana has not been studied before. In this study the bones and teeth of 84 individuals from ten Toutswe sites were analysed for osteological manifestations of disease, with the aim of assessing the general health of the Toutswe communities. These individuals were aged between newborn and 75 years old, and include both sexes. Results indicate the presence of diseases commonly found in archaeological populations such as osteoarthritis, spina bifida occulta, cribra orbitalia, porotic hyperostosis and enamel hypoplasias. One possible case of DISH was found. The frequency of lesions was comparable with or even lower than at other similar sites, such as K2 and Mapungubwe and other parts of southern Africa. The samples represent communities with relatively low levels of stress and infections. This seems to support the results of the palaeodemographic analysis, which indicated that the Toutswe people were better off than those of neighbouring K2 and Mapungubwe. Copyright © 2008 John Wiley & Sons, Ltd.     

Necropoli of Pill'e Matta Quartucciu (Cagliari,Sardinia): wild bee and solitary wasp activity and bone diagenetic factors

During the excavation at the necropolis and the laboratory studies, a variety of damage was found in the human bone remains. This damage included round holes, irregular bone destruction and irregular etching. This can be found especially in the skulls and in the long bones, and sometimes in the vertebrae and on the hands and feet. The exceptionality of this demanded meticulous study in order to define whether it was a diagenetic phenomenon of biological origin. Further studies revealed that the damage to the bones was produced by three kinds of Hymenoptera that build galleries: one of these belongs to the Sphecidae family, the other two to the Halictidae family. During the excavation of a grave it had been observed that a Halictidae had invaded the burial context and consequently contributed to the taphonomic and diagenetic process. The laboratory activities were directed towards the measurement of the damage to the human bone remains in 45 tombs. A histogram shows that the holes are not the result of accidental lesions, and the more frequent percentage values show similarities with the dimensions of holes built by the insects. The observations and the results of the study at the archaeological site enabled me to establish how significant the role of the insects had been, in particular of the Hymenoptera, in modifying the condition of the bone remains. Copyright © 2008 John Wiley & Sons, Ltd.