Ancient DNA reveals a major genetic change during the transition from hunting economy to reindeer husbandry in northern Scandinavia

The timing and origin of reindeer domestication has been highly debated. Recent molecular analyses show several mitochondrial lineages of domestic reindeer across Eurasia suggesting different origins of Fennoscandian and Siberian domestic reindeer. In order to investigate the origin of domestic Fennoscandian reindeer, we sequenced a mitochondrial control region fragment of 68 ancient reindeer remains from archaeological sites in Finnmark, the major county for extant reindeer husbandry in Norway, spanning from ca. BC 3400 to AD 1800. The majority of the Stone and Iron Age reindeer assemblages in Finnmark are from settlements in the eastern part of the county, in the Varangerfjord area. The reindeer remains from these settlements show affiliation to the large and complex Beringian haplotype cluster, found in extant reindeer from the Kola Peninsula to north-eastern Russia. A distinct haplotype shift is observed in the late medieval period, when the typical haplotype signatures of extant domestic Fennoscandian reindeer appeared in coastal regions of both eastern and western Finnmark. These haplotypes were not found among the Stone and Iron Age wild reindeer samples of Finnmark, suggesting that this population was not ancestral to extant domestic reindeer of Fennoscandia.     

Mesolithic shellfish exploitation in SW Italy: seasonal evidence from the oxygen isotopic composition of Osilinus turbinatus shells

Oxygen isotope analyses were carried out on the topshell Osilinus turbinatus from archaeological sites with the aim to investigate the temporal exploitation patterns by Mesolithic groups inhabiting coastal caves in SW Italy. In order to assess the present day O. turbinatus intra-annual δ¹⁸O variability, living specimens were collected monthly at Marina di Camerota (SW Italian peninsula). Their shell-edges were analysed and the seasonal results were compared with archaeozoological material. Our findings show that Mesolithic O. turbinatus exploitation was carried out almost exclusively during the colder and intermediary seasons (e.g. autumn, winter, spring), with very sporadic harvesting during the warmer seasons. Results suggest a possible regional exploitation pattern of O. turbinatus by Mesolithic groups inhabiting this region.     

Focused,intense and long-term: evidence for granular ark (Anadara granosa) exploitation from late Holocene shell mounds of Blue Mud Bay,northern Australia

Explanatory frameworks relating to the appearance of shell mounds and the exploitation of molluscs (particularly the sand/mudflat bivalve Anadara granosa) during the late Holocene have tended to emphasise stasis and continuity. Very few analyses have adequately investigated the intensity of human predation during the mound period and the potential effects on the particular prey species. To this end, the biological and ecological characteristics of A. granosa, the dominant molluscan species for much of the known period of occupation in the region, are considered in detail, in combination with assessing the potential for human impact through predation via the measurement of 9106 A. granosa valves from three shell mounds. In explaining long-term economic change in this region of northern Australia, the focus has been placed on the analysis of relative changes and trends through time in prehistoric resource exploitation.     

Locally diagnostic phytoliths of wild rice (Zizania palustris L.) from Minnesota,USA: comparison to other wetland grasses and usefulness for archaeobotany and paleoecological reconstructions

Wild rice (Zizania spp.) is an important native grain of north-central North America, with spiritual and subsistence significance to many native peoples. Due to lack of suitable proxies, its past distribution and pattern of use are poorly known. This study demonstrates the diagnostic value of silica phytoliths from Z. palustris as a proxy tool to detect its past occurrence in a variety of paleoenvironmental and archaeological contexts. Our main findings are: 1) Dominant wetland grass species in Minnesota produce phytoliths distinct from Zizania phytoliths; 2) Closely related Leersia oryzoides produces a few Zizania-like phytolith morphotypes; however, these potential confusers can be identified based on 2-D and 3-D morphologies; 3) Z. palustris produces a wide variety of phytolith morphotypes, many of which are only found in certain parts of the plant (e.g., Inflorescence Type 1 from the spikelet); 4) Z. palustris produces 23 locally diagnostic phytolith morphotypes that can be used to determine the presence and abundance of wild rice in modern and paleo lake sediments; and 5) Wild rice phytolith Inflorescence Type 1 is the morphotype most likely to be observed in archaeological contexts, and may be unequivocally diagnostic for the genera Zizania.     

Sweet potato (Ipomoea batatas) and banana (Musa sp.) microfossils in deposits from the Kona Field System,Island of Hawaii

Analysis of plant microfossils (pollen, phytoliths, starch grains and xylem cells) was carried out on 12 soil samples from a variety of pre-contact archaeological features in South Kona within a portion of what has been termed the Kona Field System, on the Island of Hawaii. The oldest radiocarbon ages of the sampled deposits are 1300–1625 AD and 1310–1470 AD. The pollen and phytolith evidence suggests a change from more to less trees and shrubs in the area as a result of human activity. We found phytoliths of banana (Musa sp.) leaves in most and starch grains and xylem cells of tuberous roots of c.f. sweet potato (Ipomoea batatas) in all of the samples, suggesting that the sampled features were associated with these crops and that both crops were cultivated intensively within the study area. Higher concentrations (volumetric) of starch and xylem in samples from older deposits suggest that cultivation was more intensive then. The apparent absence of starch and xylem remains of other tuberous crops archaeologically identified elsewhere in Polynesia suggests that tuberous cropping within the study area was mono-specific.     

Starch grain characterization of Prosopis chilensis (Mol.) Stuntz and P. flexuosa DC,and the analysis of their archaeological remains in Andean South America

The fruit (pods) of Prosopis (Fabaceae) are frequently recovered from pre-Hispanic Argentinian archaeological sites, suggesting that this genus was of importance in ancient economies in this region. Yet it is only recently that archaeobotanists have begun to carry out systematic research into this genus. Therefore many questions remain to be addressed concerning the food value of Prosopis fruit, and its potential contribution. This paper examines starch from the pods of two species, Prosopis flexuosa and Prosopis chilensis, for the purposes of describing and classifying their morphological features and biometrical parameters. Pods of both species were gathered from two extremes (northern and southern) of the Hualfín Valley, Catamarca, Argentina. Starch abundance is estimated and compared with that of Zea mays, an economically important plant with high concentrations of starch. This paper reveals that Prosopis pods contain very low amounts of starch compared to starch-rich edible species. Despite this, the recovery of Prosopis starch grains can be successfully used to assess archaeological tool uses. Prosopis starch grains were found to be highly variable in shape. Grain size ranged between 10 and 20 μm. Granule irregularity and the high birefringence under polarized light are two of the most important diagnostic features. No significant statistical differences were found in the structure (morphology, size and hilum) of the starch of the same species from both localities. Finally, the implications for the role of this plant in past societies are discussed.     

Reconstructing faunal migrations using intra-tooth sampling and strontium and oxygen isotope analyses: a case study of modern caribou (Rangifer tarandus granti)

Isotopic analyses of incrementally developed dental tissues can be used to reveal patterns of movement and diet in animals. However, the suitability of these methods for the reconstruction of herd movements has not yet been demonstrated. Inter-individual behavioural and isotopic variability at the herd scale, and the implications for archaeological and palaeocological applications, can only be demonstrated through the testing of modern animals. In this pilot study, dual-element isotopic profiles were created from incrementally developed dental tissues of five individuals selected from a modern herd of migratory Alaskan caribou (Rangifer tarandus granti). Enamel from second and third molars from the individuals was sequentially sampled in order to reconstruct time-series isotopic profiles. Variation in the strontium (⁸⁷Sr/⁸⁶Sr) and oxygen (δ¹⁸O_CARB) isotope ratios of sequentially sampled enamel were compared to documented herd movement patterns and local geological and environmental conditions. Four individuals displayed the same general trends, although absolute isotopic values varied. One individual displays a very different trend and may represent a behavioural outlier or an immigrant from a semi-domesticated reindeer herd. The implications of this study to herd movement reconstruction in the past are discussed.     

Oxygen isotope seasonality in a temperate estuarine shell midden: a case study from CA-ALA-17 on the San Francisco Bay,California

Seasonality determination using stable oxygen isotope (δ¹⁸O) analyses in archaeological mollusk shell has been largely limited to aquatic settings where one of the two factors that control shell δ¹⁸O – water δ¹⁸O (or salinity) and temperature – is assumed to be constant. Open coastal marine environments reflect the former situation, and tropical estuaries constitute the latter. In an effort to expand stable isotope seasonality to an ecological setting where neither variable remains constant, we present a model of annual shell δ¹⁸O cycle of aragonite deposition derived from instrumental data on salinity and temperature from San Francisco Bay, California. The predicted range of modeled shell δ¹⁸O is consistent with observed δ¹⁸O values in prehistoric and modern shells when local conditions are considered. Measurements of δ¹⁸O taken at 0 mm and 2 mm from the terminal growth margin were made on 36 archaeological specimens of Macoma nasuta from a late Holocene hunter-gatherer site CA-ALA-17, and season of collection was inferred using the shell δ¹⁸O model. We conclude that shellfish exploitation occurred through the year with the exception of fall, which may indicate scheduling conflicts with acorn harvesting or other seasonally abundant resources elsewhere. The model supports the feasibility of stable isotope seasonality studies in temperate estuaries, provided that instrumental records are available to quantify the relevant water conditions at appropriate spatial and temporal scales.     

The first fossil crow (Corvus sp. indet.) from the Early Pleistocene Nihewan Paleolithic sites in North China

We describe a three dimensional preserved right tarsometatarsus, representing the first fossil crow from the well-known Early Pleistocene Nihewan paleolithic sites in North China. The new specimen is distinguished from other known species of the genus Corvus by the combined morphological features, especially the peculiar hypotarsus. The phylogenetic significance of the arrangement of canals at the proximal hypotarsus in passerines is discussed. Although the fossil was too incomplete to warrant the erection of a new species of Corvus, it nonetheless increases the taxonomical diversity of the Nihewan fauna and improves our knowledge on Pleistocene birds in China. Finally the paleoenvironment of the Nihewan sites are discussed in terms of its faunal composition.     

Bois Laiterie revisited: functional,morphological and technological analysis of a Late Glacial hunting camp in north-western Europe

Previous exhaustive analysis of the remains at Bois Laiterie Cave resulted in the classification of this site as a Magdalenian hunting camp. However, during recent analysis of usewear-traces on selected artefacts, one of the authors [KS] noticed in the collection numerous backed points which are rather untypical for the classical Magdalenian. In light of this, a typological and technological reinvestigation of the Bois Laiterie assemblage was undertaken. Here, we present the results of this investigation and the traceological analysis. Further, implications of these results for the interpretation of the chronological, archaeo-cultural and functional position of the Bois Laiterie site within the context of the north-western European Late Glacial are discussed.     

The in situ preservation of archaeological remains: using lysimeters to assess the impacts of saturation and seasonality

Recent research has studied the effects of seasonal variability on waterlogged burial environments. In a new approach to the study of archaeological contexts, the use of controlled laboratory conditions via lysimeters has enabled new insights into the nature of short term fluctuations in saturation, and the impact that these can have on burial contexts. In addition, the protocols used have facilitated interesting insights into burial environments and the way in which fluctuations in saturation and seasonality can impact on the contained organic components within them.     

Early and mid Holocene tool-use and processing of taro (Colocasia esculenta), yam (Dioscorea sp.) and other plants at Kuk Swamp in the highlands of Papua New Guinea

Recent multidisciplinary investigations document an independent emergence of agriculture at Kuk Swamp in the highlands of Papua New Guinea. In this paper we report preliminary usewear analysis and details of prehistoric use of stone tools for processing starchy food and other plants at Kuk Swamp. Morphological diagnostics for starch granules are reported for two potentially significant economic species, taro (Colocasia esculenta) and yam (Dioscorea sp.), following comparisons between prehistoric and botanical reference specimens. Usewear and residue analyses of starch granules indicate that both these species were processed on the wetland margin during the early and mid Holocene. We argue that processing of taro and yam commences by at least 10,200 calibrated years before present (cal BP), although the taro and yam starch granules do not permit us to distinguish between wild or cultivated forms. From at least 6950 to 6440 cal BP the processing of taro, yam and other plants indicates that they are likely to have been integrated into cultivation practices on the wetland edge.     

Endangered species,archaeology, and stable isotopes: huemul (Hippocamelus bisulcus) isotopic ecology in central-western Patagonia (South America)

The management and conservation of animal species should be based upon a long-term analysis that considers its geographical distribution and feeding patterns. The study of carbon and nitrogen stable isotopes on skeletal remains provides a quantitative approach to the paleodietary reconstructions and constitutes a potent tool for comparing behavioral aspects of the fauna. In this paper we present the first set of isotopic values for modern and archaeological samples of Huemul (Hippocamelus bisulcus) from the forests of Central-western Patagonia, Chile. Contrary to initial expectations, our analysis indicates that there is no evidence of an important incidence of the canopy effect on the δ¹³Ccollagen values, which we suggest is due to an ecological selection that drives the huemul to focus its predation on open sectors within the forest. On the other hand, a wide range of δ¹⁵Ncollagen values for huemul was verified. This could reflect the N impoverishment of the forest soils and provide an interesting ecological indicator. The long-term information offered by the archaeological record provides the necessary context for decision-making conducive towards the preservation of Hippocamelus bisulcus in Patagonia.     

Environmental impacts around the time of Norse landnám in the Qorlortoq valley,Eastern Settlement,Greenland

Palynology, radiocarbon dating, and open-section stratigraphies from archaeological trenches are used to examine the impact of human activity around the time of Norse landnám on vegetation and landscape associated with a small farm (Ø34) in the Qorlortoq valley, Eastern Settlement, Greenland (61° N 45° W). Peat deposits from a mire abutting the Norse ruins revealed a discontinuous palaeoenvironmental record containing a possible hiatus from ca. AD 410–1020. Palaeovegetational data were recovered either side of this period. Pollen assemblages suggest that open Salix scrub dominated the landscape during the pre-settlement phase. The later phases of landnám resulted in the creation of hay fields and heavily-grazed grassy heath. Site abandonment is reflected by a re-expansion of Salix. This occurs shortly before the onset of deposition of a Sphagnum peat, dated to cal AD 1420–1630 (2σ) and reflecting an increase in mire surface wetness, probably in response to a deteriorating climate. Radiocarbon dates were obtained on peat and plant macrofossils sampled from either side of the proposed hiatus at two different but closely-spaced (<20 m) locations across the mire. These produced significantly different dates for the cessation of peat formation in the pre-landnám period (cal BC 2130–1770 and cal AD 240–410 respectively), but near-synchronous dates for the recommencement of peat growth (cal AD 890–1150 for peat and a probably more reliable interval of cal AD 1020–1190 based on plant macrofossils). It is suggested that this hiatus may represent the first direct evidence for peat cutting in Norse Greenland.     

Bio-archaeological evidence of olive tree (Olea europaea L.) irrigation during the Middle Ages in Southern France and North Eastern Spain

Quantitative eco-anatomical analyses were carried out on charred wood from modern olive trees (Olea europaea L.) in order to quantify influence of irrigation on wood characters and to detect irrigated olive specimens among charcoal assemblages dating back to the Middle Ages.     

Reappraisal of ‘chronospecies’ and the use of Arvicola (Rodentia,Mammalia) for biochronology

The water vole, genus Arvicola, is characterised by a broad geographic distribution throughout Europe and is widespread during the late Middle and Upper Pleistocene. This genus is used as a major biostratigraphic tool within the Quaternary. Specific determinations using the Schmelzband-Differenzierung-Quotient or SDQ have identified many chronospecies within the fossil species Arvicola cantiana (Hinton, 1910). As SDQ calculation remains limited, this study reappraises the Arvicola genus in terms of morphodiversity and morphospace using outline analysis which takes into account the tooth as a whole. Outline analysis suggests that one single species of Arvicola, A. cantiana, was present during the Pleistocene. This species shows great variability with no trends or patterns in morphospace. Thus, these results call into question the reliability of SDQ for specific determinations and throw doubt on the biochronological framework based on Arvicola.     

A short note on starch and xylem of Colocasia esculenta (taro) in archaeological deposits from Pitcairn Island,southeast Polynesia

We present results of starch analysis of archaeological deposits from Pitcairn Island. High concentrations of starch grains preserved in cell membranes, and xylem tracheary elements, consistent with introduced Colocasia esculenta (taro) were found. Because of limited age control, we are uncertain if the microfossils are prehistoric. Problems associated with identifying taxa with small starch grains in extractions from weathered deposits are highlighted.     

A deciduous and permanent dental wear stage system for assessing the age of Trachypithecus sp. specimens (Colobinae, Primates)

Although Colobine monkeys are one of the most common prey hunted by Southeast Asian prehistoric humans, no data concerning tooth eruption and wear are available for these species. Dental wear eruption and attrition are used for attributing ages to individual fossils in order to construct mortality curves, and are also useful in reconstructing fossil life histories. Such wear sequences partly exist for Cercopithecines. Although Cercopithecines and Colobines are both bilophodont, they present significant differences in terms of diet, detailed tooth morphologies and occlusion. This paper aims to formulate a guide for the dental eruption and attrition of extant Trachypithecus specimens, the most folivorous of all the Colobines. We also propose to calibrate the eruption and wear stages that we define here with absolute ages available in the literature.     

The hunted hunter: the capture of a lion (Panthera leo fossilis) at the Gran Dolina site,Sierra de Atapuerca,Spain

Many Pleistocene caves and rock shelters contain evidence of carnivore and human activities. For this reason, it is common to recover at these sites faunal remains left by both biological agents. In order to explain the role that carnivores play at the archaeological sites it is necessary to analyse several elements, such as the taxonomical and skeletal representation, the age profiles, the ratio of NISP to MNI, the anthropogenic processing marks on the carcasses (location and purpose of cutmarks and burning and bone breakage patterns), carnivore damage (digested bones, location and frequencies of toothmarks and bone breakage), length of the long bones, frequencies of coprolites and vertical distribution of the faunal remains, inter alia. From this, the documentation of carnivores in a faunal assemblage with a clear anthropogenic component can be understood from three main phenomena: (1) the carnivores as accumulators and the use of the site as a den; (2) carnivores as scavengers of hominid refuse and; (3) carnivores as hominids’ prey. Of these three phenomena, the last one is the least documented at the Middle Pleistocene sites. From this perspective, here we present the case of the anthropogenic use of a lion (Panthera leo fossilis) from level TD10-1 of Gran Dolina (MIS 9, Sierra de Atapuerca, Burgos, Spain). The lion bone remains show signs of direct interaction between this big cat and human groups that occupied Gran Dolina in these chronologies. From this perspective, the aim of this paper is to contribute to the knowledge of the role developed by large carnivores in the anthropogenic contexts and to provide data on human use of these predators at the European Middle Pleistocene sites.     

Fishing ancient Lake Baikal,Siberia: inferences from the reconstruction of harvested perch (Perca fluviatilis) size

Fishing was the foundation for many of the world's foraging peoples and was undertaken using a variety of technologies. Reconstructing fishing technologies can be difficult because these tools were often made of perishable materials. Here we explore fishing technologies employed at the Ityrkhei site on Lake Baikal, Siberia. Specifically, we employ regression analyses to reconstruct the sizes of perch (Perca fluviatilis) captured through time at the site. Our analyses demonstrate that almost no juvenile perch were taken, suggesting some selectivity in harvest. We suggest this selectivity is most consistent with the use of relatively large gauge nets or traps. Such mass harvesting technologies may have been important elements of the subsistence economies of Lake Baikal's foraging peoples throughout much of the Holocene.