Quantitative computerized tomography for the diagnosis of osteopenia in prehistoric skeletal remains

In this study, we examine the value of bone density assessed by computed tomography (QCT) of the right tibia in the diagnosis of low bone mass in prehistoric bones. Trabecular bone mass (TBM) was assessed by histomorphometry in undecalcified bone sections of a small part of the proximal epiphysis of the right tibia of 78 prehispanic individuals. Bone density was also assessed in the proximal epiphysis of the right tibia, in an area of cancellous bone immediately adjacent to the wedge which was destined to histomorphometry, with the aid of a Tomoscan 60 TX™ (Philipps Medical System, Eindhoven, The Netherlands) using a phantom of hydroxyapatite included in plastic resin at three known concentrations (50, 100 and 200 mg/cc) and ethanol as fat equivalent. Bone density (as bone hydroxyapatite concentration in milligram per cubic centimeter) was calculated by means of a specific software tool (QCT Bone Mineral Analysis System, Image Analysis, California). We compared bone density assessed with QCT with TBM in these individuals, and also, with the results obtained from a modern control group. We calculated the median TBM of the prehispanic sample and tested the sensitivity, specificity, and overall accuracy of QCT in diagnosing TBM values below the median. We later performed the same analyses on 24 more prehispanic individuals. Both QCT (t = 5.61, p < 0.001) and TBM (t = 3.79, p < 0.001) were significantly lower among the prehispanic individuals than among the control ones. QCT showed a significant relationship with TBM (r = 0.41, p < 0.001). QCT values below 100 mg/cc serve to establish a diagnosis of low TBM values with a sensitivity of 82.1% and a specificity of 41%. In the test group sensitivity was 83.3% and specificity, 50%.     

Identification of fragmentary bone from the Pacific

We explore bone microstructure for taxonomic identification of archaeological bones too fragmentary to permit secure identification on morphological grounds. Backscattered electron (BSE) imaging is used to observe bone tissue types and the arrangement of vascular canals, and to facilitate quantification of osteonal canal dimensions. Examination of known examples of relevant taxa (humans [n = 8], pigs [n = 4] and dogs [n = 4]) shows significant differences among them. When the results of this examination are applied to a blind test of modern and archaeological specimens (humans [n = 8], pigs [n = 2]), 100% of specimens are identified correctly. The approach is applied to 13 morphologically unidentifiable fragments from Hawai’i and Fiji to evaluate its potential for identifying bone tools and to increase the number of samples available for dietary analysis. Potential applications of the approach for other contexts are discussed.     

Calculus,periodontal disease and tooth decay among the prehispanic population from Gran Canaria

In the prehispanic Gran Canaria there are some anthropological differences between the coastal inhabitants who buried their dead mainly in tumuli, and those from the central mountains, mainly buried in caves. Some data, as the prevalence of auricular exostoses, and a different bone Ba/Sr ratio support the view that there were differences in economy and diet between both groups of islanders. Moreover, the proportion of carious teeth was significantly higher among the population buried in caves. In the present study we analysed the prevalence of dental calculus, periodontal disease and antemortem tooth loss in remains of 791 individuals belonging to the anthropological collection of the Museo Canario (Las Palmas). Calculus deposition was very frequent (88.51%), no differences existing between men and women or between those interred in tumuli or in caves. Age at death was the only parameter independently related to calculus deposition by stepwise multivariate analysis. Periodontal disease was observed in 66.78% of the population, significantly more in men (χ² = 4.88, P = 0.027). No differences existed between individuals buried in tumuli and in caves. Antemortem teeth loss was observed in 64.73% of individuals, no differences existing between men and women or between those interred in tumuli or in caves. There was a significant association between calculus and periodontal disease (χ² = 18.07, P < 0.0001). Both caries (χ² = 8.40, P = 0.004) and periodontal disease (χ² = 44.96, P < 0.0001) were associated with tooth decay. However, the proportion of teeth with calculus deposition (in relation to observed teeth) was significantly higher among the population buried in tumuli (Z = 3.18, P = 0.001), although no differences were observed when the proportion of antemortem lost teeth and alveoli with periodontal disease were compared among people buried in tumuli and in caves, but women showed significantly lower proportions of alveoli with periodontal disease and antemortem tooth decay. These data suggest that the population buried in caves had a different dietary pattern to that of those buried in tumuli, since calculus deposition -more frequent in the latter-may be related to the consumption of proteins. The results also point to the existence of differences in diet between men and women.     

Climate,people and faunal succession on Java,Indonesia: evidence from Song Gupuh

Song Gupuh, a partially collapsed cave in the Gunung Sewu Limestones of East Java, Indonesia, contains over 16 m of deposits with a faunal sequence spanning some 70 ka. Major changes in the range of animals represented show the impact of climate change and humans. The Terminal Pleistocene and Early Holocene was a period of maximum biodiversity. Human use of Song Gupuh and other cave sites in the region also intensified significantly from ca. 12 ka, together with a new focus on exploitation of small-bodied species (macaque monkeys and molluscs), the first evidence for import of resources from the coast, and use of bone and shell tools. Human activity, especially after the onset of the Neolithic around 2.6 ka, subsequently contributed to a progressive loss of many species from the area, including tapir, elephant, Malayan bear, rhino and tiger, and this extinction process is continuing. We conclude by discussing the biogeographical significance of Song Gupuh in the context of other sites in Java (e.g. Punung, Wajak) and further afield (e.g. Liang Bua).     

Frogs as a part of the Eneolithic diet. Archaeozoological records from the Czech Republic (Kutná Hora-Denemark site, Řivnáč Culture)

Within the site of Kutná Hora-Denemark (Kutná Hora distr., central Bohemia – Eneolithic, ?ivná? Culture, 3000–2800 BC), 893 frog bones or bone fragments were present in five archaeological features. All identified specimens are of the Common Frog (Rana temporaria Linnaeus, 1758). Males predominate, according to humeral morphology, and only adult individuals are present. The most important findings come from feature 36, where an accumulation of 739 frog bones (MNI = 123) were found, of which 10% (NISP) were burned. This and other contextual evidence indicates that they relate to activity of the Eneolithic people in the settlement. The predominance of hind leg bones and other circumstantial evidence suggest that the frogs were part of the human diet. Behavioural studies of this species suggest that the frogs were gathered from small ponds during the months of March or April. Similar findings are rare.     

Marine electrical resistivity tomography for shipwreck detection in very shallow water: a case study from Agropoli (Salerno,southern Italy)

A marine electric resistivity survey was carried out over a submerged beach along the Agropoli shore (Salerno, Italy) to detect buried objects of archaeological interest below the sandy seabed. We found a shipwreck, a military vessel that probably sunk during the Salerno landing operations of the allied forces in the Second World War. Resistivity data provide information on the vertical and horizontal extension of the shipwreck, which is characterized by very low calculated resistivity values (about 2–5 ohm m). Such values differ significantly from the sand and the bedrock values (5–40 ohm m). Although the presence of the shipwreck is clearly visible from geoelectric data, the joint application of electric, magnetic and multibeam bathymetric techniques reduces the ambiguities inherent in each method. As shown in the Electrical Resistivity Tomography (ERT) and confirmed by the Digital Elevation Model (obtained from the processing of bathymetric data), the shipwreck extends more than 30 m in NE–SW direction and it is about 13 m wide. The global extension of the relic is consistent with the magnetic data, that are characterized by a magnetic anomaly with an amplitude of about 1800 nT and similar dimension, as inferred from the estimation of source boundaries obtained from the computation of the analytic signal. The results of our survey encourage the use of marine geoelectrical methods for the detection of buried archaeological targets, particularly in locations where the use of seismic prospecting is not effective (e.g. very shallow water with sandy sea-bottoms). The integration of different geophysical methods allows to better define the extension, depth and thickness of buried objects, suggesting that such an approach is the most effective for underwater archaeological investigations.     

An assessment of morphological criteria for discriminating sheep and goat mandibles on a large prehistoric archaeological assemblage (Kerma,Sudan)

This paper presents an assessment of all known dental and mandibular morphological criteria for differentiating sheep (Ovis aries) and goats (Capra hircus) using for the first time an archaeological sample of complete caprine skeletons (90 sheep and 13 goats) from burials at Kerma (Sudan, 3rd and 2nd millennia BC). The species determinations were assessed using cranial and post-cranial morphological criteria. Consequently, the reliability (percentage of correct determination) and efficiency (complementary percentage of intermediate scores i.e. neither sheep nor goat) of 38 individual dental criteria could be fully assessed using a prehistoric homogeneous domesticate population. We demonstrate that, for this sample, individual criteria for lacteal teeth are more reliable for sheep (sheep: 95 ± 3%) than the adult premolars (85 ± 5%) and molars (sheep: 88 ± 2%), whereas for goats premolar criteria were more reliable (83 ± 12%). For efficiency, lacteal dental criteria are better (goat: 97 ± 5%; sheep: 95 ± 3%) than those for premolar (goat: 85 ± 10%; sheep: 79 ± 5%) and molar (goat: 82 ± 6%; sheep: 83 ± 2). We also demonstrated that most isolated teeth can be determined with less than 10% error. However, on average, within specific age classes (0–1 year, 1–4 years and more than 4 years), isolated teeth increased in reliability and decreased in efficiency. The average reliability of the criteria for complete mandibles for each age class for goats and sheep was 100%, when the efficiency was 67, 40 and 50% for goats and over 90% for sheep. This is due to the effect of age on the efficiency of isolated criteria and the poor performance of specific criteria mainly those P3, M1 and M2. We conclude that separate species kill-off profiles are possible. The effect of age on dental criteria would not significantly change the interpretation for specific subsistence strategies focused on one particularly species.     

Tracking changes in bone fracture morphology over time: environment, taphonomy, and the archaeological record

Zooarchaeologists have often employed studies of bone fracture morphology as a means of understanding past human cultural activity, and various methodological approaches have been developed for analyzing archaeological broken bone assemblages. It is widely understood that bones degrade over time, however, few studies have attempted to define and quantify the rate at which bones degrade and fracture morphologies change. This study examines degradation in frozen bones (−20 °C) and bones exposed to hot (40 °C) dry conditions. These two simulated environmental conditions represent extreme real-world climates, and allow for an actualistic understanding of the rates of degradation that bones experience in nature. When frozen, bones degrade slowly but significantly, and demonstrate measurable differences in samples frozen for 1, 10, 20, 40, and 60 weeks. In hot, dry conditions, bones degrade very quickly, and demonstrate measurable differences after 1, 3, 7, 14, and 21 days. These data allow for a more detailed understanding of the relationship between the cultural and natural processes that result in bone fracture, and the time period during which bones can be expected to maintain fresh fracture characteristics. This research also has implications for understanding human subsistence and survival strategies and for interpreting the archaeological record.     

Osteoarthritis and body mass

Osteoarthritis is the second most common pathology found in anthropological collections. Although a great deal is known about osteoarthritis, it is not yet known whether in skeletal populations there is a correlation between osteoarthritis and body mass. In this study, lower limb and spinal osteoarthritis scores were taken to determine whether body mass and femoral length correlate with osteoarthritis on weight bearing joints. Osteoarthritis was measured using a four-point ordinal scale on a sample of 114 adult prehistoric Californian Amerinds. Body mass was calculated from femoral head breadth; femoral length was measured using standard procedures; and age and sex were determined through standard osteological procedures. Using Spearman correlations, body mass and femoral length did not correlate significantly with any of the osteoarthritis variables. Age correlated significantly with nearly all of the osteoarthritis variables (hip, r = 0.507; knee, r = 0.528; cervical, r = 0.513; thoracic, r = 0.647; lumbar, r = 0.507, P-values < 0.001); and body mass and femoral length correlated with sex (r-values = 0.835 and 0.654, P-values < 0.001). With age and sex controls, body mass negatively correlated with the hip osteoarthritis variable (r = −0.202, P < 0.05), but not with any of the other osteoarthritis variables. Results concur with previous findings in the anthropological literature and highlight complexities of osteoarthritis etiology.     

Reburial of shipwrecks in marine sediments: a long-term study on wood degradation

Historical shipwrecks in marine environments are continuously decomposed by wood-degrading organisms, adapted to this specific environment. To protect the wrecks from degradation and to preserve the unique remains for future generations, reburial of wrecks using various covering materials has been suggested as a long-term preservation method. The following experiment was carried out to study the bio-protective effect of sediment. Sound oak, pine, and birch samples were buried above and within marine sediment in Marstrand harbour, and retrieved for analysis after 6, 12, 24, and 36 months. Macroscopic as well as light-microscopic examinations were carried out on each occasion. Marine borers (shipworm, Limnoria), soft rot and tunnelling bacteria were wood degraders immediately above the seabed; soft rot, tunnelling bacteria, and erosion bacteria were active 10 cm below the seabed; erosion bacteria were the only degraders at a depth of 43 cm below the seabed. The wood species had different durability towards the decay agents. After 3 years, wood samples above the seabed were totally decomposed, whereas wood was highly protected at 43 cm depth in sediment. In the sediment, decay decreased significantly with depth of burial. The results suggest that reburial of shipwrecks in marine sediment can be recommended as a simple and efficient method for long-term preservation of the wooden cultural heritage.     

Probing dietary change of the Kwädąy Dän Ts'ìnchį individual,an ancient glacier body from British Columbia: II. Deconvoluting whole skin and bone collagen δC values via carbon isotope analysis of individual amino acids

The remains of the Kwäd?y Dän Ts'ìnch? individual, a frozen male human, were recovered from a retreating glacier within the Tatshenshini-Alsek Park in British Columbia in August 1999. In order to provide information on both the geographical origin of this individual and low long he spent in the remote interior region prior to his death, molecular analysis and compound-specific carbon isotope analyses were performed on individual amino acids purified from his skin and bone. Gas chromatographic quantification of constituent amino acids of both tissues revealed a molecular distribution characteristic of collagen, dominated by glycine and to a lesser extent proline, hydroxyproline and alanine. Chiral gas chromatography indicated that protein preservation in both tissues was exceptional. Carbon isotope analysis of a faunal assemblage from an earlier prehistoric site from southern British Columbia provided reference dietary amino acid δ¹³C values for terrestrial (deer and domestic dog) and marine species (salmon and sealion), showing clear separation in all amino acids, particularly glycine which was extremely ¹³C-enriched in the marine animals. The distinction between terrestrial and marine organisms was increased by exploring Δ¹³C_{Glycine-Phenylalanine} values (6.6 ± 0.6‰ and 15.0 ± 2.1‰, respectively), which were higher in the latter by approximately 8‰, mirroring the increased δ¹⁵N_{Bulk collagen} values observed for the marine animals (R² = 0.78; p < 0.001). The Kwäd?y Dän Ts'ìnch? individual's bone had a similarly elevated Δ¹³C_{Glycine-Phenylalanine} value of 15.6 ± 1.0‰, indicating his extreme reliance on marine dietary resources throughout early life. The skin amino acid δ¹³C values were consistently lower than those observed for bone, with a concurrently lower Δ¹³C_{Glycine-Phenylalanine} value of 12.7 ± 0.9‰. The shift between the carbon isotope composition of bone (long-term diet) and skin amino acids (short-term diet) confirmed a sudden divergence away from marine food sources in the last months of life, consistent with his discovery 80 km inland.     

Identifying sword marks on bone: criteria for distinguishing between cut marks made by different classes of bladed weapons

Swords have been one of the major weapons used in violent conflicts for much of human history. Certain archaeological situations, especially those dealing with the recovery and analysis of battle casualties, may raise questions about what type(s) of bladed weapon was used in a particular conflict (e.g., the battle of Kamakura, Japan, AD 1333; the battle of Wisby, Sweden, AD 1361; the battle of Towton, England, AD 1461). Little work has been done, however, on developing criteria to differentiate sword cut marks from other types of cut marks, or to distinguish between marks created by different sword types. To develop such criteria, bovine tibiae (n = 7) were struck using six different types of bladed weapon and the resulting marks (n = 92) were analyzed. Eight traits describing the morphology of the cut mark – such as shape, the presence and unilateral/bilateral state of flaking and feathering, the presence of bone shards, associated breaks, etc. – are defined and related to blade type used. Sword marks were found to be easily distinguishable from knife marks. The variation in marks made by different sword types is significantly correlated with differences in blade weight (p < 0.0001), grip (p < 0.0113), and sharpness (p ≤ 0.0179). The criteria and analyses developed and implemented in this study will be of use to researchers in forensics and osteoarchaeology who want to infer bladed weapon type from marks on bones.     

Going underground: experimental carbonization of fruiting structures under hearths

We show that carbonized fruits and seeds recovered from Middle Stone Age deposits in rock shelters are likely to have been carbonized as part of post-depositional processes. We buried indigenous South African fruits, nuts and seeds at pre-determined depths and distances from the centers of experimental fires. The cold ashes of the hearths and the sand surrounding them were subsequently excavated, using standard archaeological techniques and dry-screening. The fruiting structures from the oxidizing part of the fire were burnt to ashes, whereas those buried in sand under anoxic conditions survived in varying forms. Those buried 5 cm below the center of the fire were carbonized; those buried 10 cm below the center of the fire were dehydrated; and those 5 cm and 10 cm below the surface at the outer edge of the fire were unaffected. Size, moisture or oil content of the original fruit or seed did not appear to influence whether or not carbonization took place. Temperatures recorded 5 cm below the experimental fires suggest that the carbonization occurred at or before a maximum temperature of 328 °C, and also at lower maximum temperatures (152 °C) that were maintained for long periods. Even when the quantities of a particular wood are controlled, open fires may produce variable underground temperatures and the temperatures below ancient hearths would have been equally variable. We suggest that Cyperaceae (sedge) nutlets, the most numerous fruiting structures in the Sibudu Middle Stone Age archaeobotanical assemblage, occur in the shelter as a result of human activity, subsequent burial, and accidental carbonization when hearths were built directly above the buried nutlets.     

A pilot study into the effects of X-ray and computed tomography exposure on the amplification of DNA from bone

This research investigates the intersection of radiography and aDNA, two commonly used methods in bioarchaeology. The goal of this project was to investigate the effects of radiation on the ability to amplify DNA from bone. Bones (n = 124) from domestic pig (Sus scrofa) feet were randomly sorted into a control group and four treatment groups: (1) single exposure X-ray; (2) single exposure CT; (3) multiple exposures X-ray; and (4) multiple exposures CT. Number of PCR cycles required to amplify DNA in 100 bp, 200 bp and 400 bp segments were used as a proxy for the amount of available DNA. In the 200 bp CT sample, distal phalanges required significantly more amplification cycles than did the other bones and were removed from analysis. Results suggest that in general radiation exposure fragments DNA thereby decreasing the amount that is amplifiable. While these results are suggestive, further research is required to elucidate the degree to which radiation fragments DNA in archaeological specimens.     

Stable isotope evidence of human diet at the Nukdo shell midden site,South Korea

Carbon and nitrogen stable isotope analysis was undertaken on bone collagen extracted from archaeological human (n = 48) and animal (n = 45) skeletons from the Nukdo site, Location I C, South Korea. This shell midden and grave site is dated from the late Mumun (550–300 BC) to early Iron Age (300 BC-1 AD) periods. The herbivorous mammals fell within the range of C₃ consumers, with average values of δ¹³C = −21.0 ± 0.5‰ and δ¹⁵N = 3.6 ± 0.5‰ for deer (n = 16) and δ¹³C = −20.6 ± 0.5‰ and δ¹⁵N = 4.5 ± 2.0‰ for wild boar (n = 17). Humans from this site averaged δ¹³C = −18.3 ± 0.4‰ and δ¹⁵N = 11.2 ± 0.7‰ for adults (n = 15) and δ¹³C = −18.7 ± 0.7‰ and δ¹⁵N = 12.5 ± 1.1‰ for juveniles (n = 33). These δ¹³C values indicate that there was no significant input of C₄ plants in the human diets and this may be associated with the spread of rice agriculture in the Mumun period. Human bone collagen δ¹³C and δ¹⁵N values indicate that there was some consumption of marine foods, although the main protein sources were from terrestrial foods. The isotope data demonstrate that the humans at Nukdo had mixed diets that included marine and terrestrial protein, including C₃ plants such as rice. Finally, the isotope results from the juveniles indicate that weaning occurred before the age of 1.5 years in this period.     

Magnetic detection of ship ballast deposits and anchorage sites in King Herod's Roman harbour,Caesarea Maritima,Israel

Ballast stone deposits are a common feature of sediments in ancient harbour basins but are often overlooked as a potential source of archaeological information. Recent geophysical investigations at Caesarea Maritima in Israel have discovered a thick, laterally extensive ballast layer in the area seaward of the 1st c. BC Roman harbour. The ballast deposits were identified by low-relief mounds on the seabed with elevated magnetic intensities. Jet probing and excavation of magnetic anomalies at several locations revealed a 20–60 cm thick rubble layer containing large quantities of Late Roman and Byzantine pottery, local sedimentary boulders (kurkar sandstone, limestone cobbles) and foreign igneous and metamorphic boulders (granite, schist, volcanics; ca. 50%). The foreign boulders and pottery identify the rubble layer as ballast and ships refuse jettisoned by merchant ships outside the harbour. The strong magnetic contrast between the ballast deposits and the natural seabed sediments is attributed to the high magnetic susceptibility (>10⁻³ SI) of crystalline boulders and pottery materials within the ballast rubble.     

Sorting the butchered from the boiled

Is it possible to identify cooked, rather than burnt, bone? Mild heating (≤100 °C,1 h) – typical of cooking – does not lead to detectable changes in any biochemical parameter of bone yet measured. If it is only possible to detect charred bone, how is it possible to detect cooking in the archaeological record? In a previous paper (Koon et al., 2003, J. Arch. Sci.), we used a Transmission Electron Microscopy (TEM) based approach to investigate changes in the organization of the bone protein, collagen, as it is heated, using bone from heating experiments and short term burials. The work revealed that mineralized collagen, despite requiring aggressive treatment to gelatinise the protein (e.g. 90 °C, 240+ h), readily accumulates minor damage. We believe that the presence of mineral matrix stabilises the collagen enabling the damage to accumulate, but preventing it from causing immediate gelatinisation. Once the mineral is removed, the damage can be observed using appropriate visualization methods.     

AMS C dating of early human occupation of southern South America

The time of appearance of a persistent and demographically-viable hunter-gatherer population in late Pleistocene southern South America must be determined by evaluating evidence from as large as possible a sample of candidate archaeological sites in the region. We co-ordinated the AMS dating of multiple bone and charcoal samples from previously-excavated strata at the following sites: Arroyo Seco 2, Paso Otero 5, Piedra Museo, and Cueva Tres Tetas (all in Argentina), and Cueva del Lago Sofia 1 and Tres Arroyos (both in Chile). With one possible exception, we did not obtain new results to confirm earlier observations of pre-Clovis-age cultural activity at any of the sites considered in this study. The possible exception, Arroyo Seco 2, is considered in detail elsewhere [Politis G., Gutierrez M.A., Scabuzzo, C. (Eds), in press. Estado actual de las Investigaciones en el sitio 2 de Arroyo Seco (región pampeana, Argentina). Serie Monográfica INCUAPA 5. Olavarría]. However, our results for the samples which were the most preferred indicators of cultural events (hearth charcoal and cut-marked bone) confirm that people were in the southern cone of South America at or soon after 11,000 BP (13,000 cal BP). Considered alongside recent age estimates for the Clovis culture in North America, these results imply the contemporaneous emergence of a consistent and archaeologically-robust human occupation signal at widely-separated locations across the Western Hemisphere. Such findings suggest that Palaeoindian demic expansion may have involved more than one terminal Pleistocene dispersal episode.     

Severe lead contamination among children of samurai families in Edo period Japan

This paper presents evidence for severe lead contamination among children of samurai families living in a castle town in Edo period Japan (1603–1867). Excavated rib bones were analyzed by atomic absorption, and soft X-ray roentgenograms of long bones were taken. The median values of lead concentration in the bones of children 3 years of age and under(1241.0 μg Pb/g dry bone) and 4–6 years of age (462.5 μg Pb/g dry bone) were significantly higher than those of adult males (14.3 μg Pb/g dry bone) and females (23.6 μg Pb/g dry bone) (p < 0.001). In addition, that of children over 6 years of age (313.0 μg Pb/g dry bone) was significantly higher than those of adult males (p < 0.01) and adult females (p < 0.05). The median value of lead in the bones of children 3 years of age and under was over fifty times higher than that of their mothers (adult females). Hypertrophy was seen in the long bones of five samurai children. In this area, lead lines or lead bands were distinguished by soft X-ray roentgenogram. Samurai children suffered from severe lead contamination in Edo period Japan. When the mothers were nursing their children, the children might have ingested their mother’s white lead non-selectively.