MtDNA origins of an enslaved labor force from the 18th century Schuyler Flatts Burial Ground in colonial Albany,NY: Africans,Native Americans,and Malagasy?

A burial ground located in the Town of Colonie, NY along the Hudson River revealed fourteen individuals dated from the 17th through the early 19th centuries. Bioarchaeological analysis suggested some of these individuals were of African ancestry who had worked and died on the property owned by the prominent Schuyler family. Mitochondrial DNA (mtDNA) analysis was carried out on skeletal remains of seven adults using restriction fragment length polymorphism typing and direct sequencing of the control region to infer their origins and relatedness. Results show that none of the individuals were maternally related, with four individuals identified as African haplogroup L, one identified as Native American haplogroup X, and two identified as haplogroup M and M7. Individuals of African ancestry correlate with published mtDNA data on African Americans and their geographical origins corroborate with the various exit points during the African slave trade to New York State. Individuals identified as haplogroup M7 and M resemble lineages found in Madagascar. Historical documents suggest several hundred people were imported from Madagascar through illegal trading to New York by the end of the 17th century. This study highlights the diverse origins of the enslaved labor force in colonial New York and contributes to our understanding of African American history in the northeast.     

DNA analysis of archaeological sheep remains from China

Recent research has thrown considerable light on the history of the domestic sheep, but has not extended to ancient sheep specimens. In the present study, ancient DNA analysis was carried out on eight archaeological sheep remains recovered from Erlitou archaeological site in Henan Province (ca. 2100–1800 B.C.) to explore the genetic structure of ancient sheep and the phylogenetic relationship between ancient and modern sheep. We analyzed the control region sequences and coding regions of mitochondrial DNA from the remains by direct sequencing and restriction fragment length polymorphism analysis, respectively. Our results reveal that all ancient sheep belong to lineage A defined by modern sheep sequences. Phylogenetic analysis shows that neither argali (Ovis ammon) nor urial (Ovis vignei) mtDNA is closely related to Erlitou ancient sheep. In addition, our results suggest that ancient DNA analysis can serve as a powerful tool in tracing prehistoric population movement.     

A bioarchaeological study of a Western Basin tradition cemetery on the Detroit River

Archaeological monitoring of construction in a Windsor city park on the Detroit River led to the discovery of an isolated cemetery containing the remains of eight individuals assigned to the Late Woodland Western Basin Tradition. At the request and consent of the contemporary First Nation community, tissue samples from five individuals were subjected to radiocarbon dating, mtDNA, and stable isotope analysis to confirm cultural affiliation and further understand the subsistence practices of these people. Radiocarbon dating placed the cemetery at the transition from the Younge phase (AD 900–1200) to the Springwells phase (AD 1200–1400). The stable carbon and nitrogen isotope results provide an unexpected but fuller understanding of Late Woodland Western Basin Tradition subsistence. All individuals were as enriched in carbon as those found on Iroquoian horticulturalist sites to the east, suggesting a very high reliance on maize. Nitrogen isotope values indicate that the protein component of the diet was comprised largely of high trophic level food sources, likely fish. An in situ osteological analysis identified a high number of carious lesions in the visible teeth, also suggesting a diet high in carbohydrates. The mtDNA findings support the antiquity of the Western Basin presence in Northeast North America through genetic links with the Hind Site, an Archaic site in southern Ontario. These results underscore the importance of such studies for providing novel insight into the archaeological histories and lifeways of this distinct Late Woodland tradition. This study also emphazises the need to work with descendant communities to provide them with information on the past that reflects their distinct heritage in the lower Great Lakes region.     

Pretos Novos: Evidence for African Oral Hygiene Practices in Brazil, 1769–1830

Fine, polished abrasion of teeth provides evidence for use of traditional oral hygiene practices in 32 fragmentary dentitions from a cemetery for newly arrived enslaved Africans who died before leaving Valongo, the slave port and market of Rio de Janeiro. We infer that chewing sticks were used before these people were enslaved. Cosmetic dental modification and abrasion of tooth roots occur in some individuals. High caries frequency, tooth loss and hypercementosis characteristic remains of enslaved Africans with longer residence in the New World were not found in this unique collection. We review evidence that the practice of using chewing sticks persists in some regions of the Americas. Copyright © 2012 John Wiley & Sons, Ltd.     

Examining dispersal mechanisms for the translocation of chicken (Gallus gallus) from Polynesia to South America

DNA sequencing and radiocarbon dates from the archaeological site of El Arenal-1 (Arauco Peninsula, south central Chile) suggest that Polynesians may have introduced chicken (Gallus gallus) to South America prehistorically (A.D. 1300–1420). The Chilean specimen identified has the exact same amplified mtDNA pattern as samples recovered from archaeological sites in Samoa and Tonga, but is only one base different from prehistoric chicken samples from Tonga (n = 1), Niue (n = 1), Easter Island (n = 1 of 6), and Hawai'i (n = 1). To examine the possible dispersal mechanisms of chicken between Polynesian islands and the Americas, we conducted computer simulations of seafaring from these six island groups and the Tuamotus, as well as return voyages. Simulation results from sailed vessels suggest that in certain months, landfall on the Pacific Coast of South America from Samoa and Tonga was fairly successful at around 40% (particularly in March for the former and January/February for the latter). For Niue, sailed vessels were 21% successful in January with only 2% success in December. Sailed vessels from the Tuamotus (a proxy for closer island groups to the west with evidence for chicken such as the Cook Islands) were successful in January and December, with 15% and 23% of the vessels making landfall in South America. Vessels sailing from all simulations heading east from Easter Island resulted in landfall. Hawai'i had only a 1% success rate if sailing in January, but high numbers of vessels reached southern Mexico and Central America in August and September. Most landfalls occurred much farther north than Chile, in Ecuador and Peru. Unsuccessful voyages from all of the islands other than Easter Island and Hawai'i often made landfall on islands to the east. Return voyages from Chile were not possible or extremely rare during most times of the year. Overall, seafaring simulations suggest that Polynesians may not have initially landed in close proximity to El Arenal-1—contemporaneous or even earlier archaeological evidence of translocated chicken remains may indeed be found further north. In addition, if Polynesians found it difficult or impossible to return to their homeland, the possibility exists that co-mingling between Polynesians and South American groups resulted in offspring that carried an admixture of genetic traits.     

Ancient DNA,a Neolithic legging from the Swiss Alps and the early history of goat

Ancient DNA from a Neolithic legging (1st half of the 3rd millennium BC) found at Lenk, Schnidejoch (2750 m a.sl.) in the Swiss Alps has demonstrated, that modern distribution of genetic variation does not reflect past spatio-temporal signatures. The legging was made from the skin of a domestic goat (Capra hircus), belonging to the caprine haplogroup B1, which is marginal in Europe today, but represents a third highly diverse goat haplogroup entering Europe already in the Neolithic. Population expansion of lineage B therefore happened more than 4500 years ago, but their members were at some point almost completely replaced by goats of today's common A and C haplogroups.     

Phylogeography of einkorn landraces in the Mediterranean basin and Central Europe: population structure and cultivation history

Einkorn (Triticum monococcum L.) was one of the first cereals to be domesticated in the Old World ca. 10,000 years ago and to spread towards Europe and North Africa. Its cultivation declined before the Iron Age and it remains today only as a relic crop in remote areas. To investigate if the geographic distribution of genetic diversity in modern einkorn landrace accessions could be informative about the movement of this crop during prehistory, we genotyped 50 accessions of einkorn from Europe, North Africa and the Near East. Using nuclear and chloroplast microsatellites and clustering methods, we detected two main gene pools in einkorn. The distribution of these lineages revealed differences between accessions from Morocco and the Iberian Peninsula from the rest of Europe and the Near East and suggests different regional dynamics in the spread of this crop.     

Ancient DNA typing of archaeological pig remains corroborates historical records

The recent increase in both the abundance and taxonomic range of DNA sequence data in public repositories makes it possible to determine the maternal origin of lineages of faunal archaeological material by characterizing its mitochondrial DNA. Among the most commonly represented taxa are domesticated animals, for which extensive genetic characterization has revealed high levels of genetic diversity and (in at least some cases) strong phylogeographic clustering. Such information has significant implications not only for characterizing important aspects of the occupation history of archaeological sites, but also in providing novel insights into colonisation history and the scale and scope of trade and exchange networks. This can be done through studying the origins and dispersal of proxy organisms such as commensal and domesticated animals, as well as economically important wild fauna. To illustrate this approach, we compare historical records of maritime movement of people and pigs from two sites on Lord Howe Island, Australia, to phylogeographic results of DNA extracted from pig bones.     

Y chromosomes of self-identified Syeds from the Indian subcontinent show evidence of elevated Arab ancestry but not of a recent common patrilineal origin

Several cultural or religious groups claim descent from a common ancestor. The extent to which this claimed ancestry is real or socially constructed can be assessed by means of genetic studies. Syed is a common honorific title given to male Muslims belonging to certain families claiming descent from the Prophet Muhammad through his grandsons Hassan and Hussein, who lived 1,400 years ago and were the sons of the Prophet’s daughter Fatima. If all Syeds really are in direct descent from Hassan and Hussein, we would expect the Y chromosomes of Syeds to be less diverse than those of non-Syeds. Outside the Arab world, we would also expect to find that Syeds share Y chromosomes with Arab populations to a greater extent than they do with their non-Syed geographic neighbours. In this study, we found that the Y chromosomes of self-identified Syeds from India and Pakistan are no less diverse than those non-Syeds from the same regions, suggesting that there is no biological basis to the belief that self-identified Syeds in this part of the world share a recent common ancestry. In addition to Syeds, we also considered members of other hereditary Muslim lineages, which either claim descent from the tribe or family of Muhammad or from the residents of Medinah. Here, we found that these lineages showed greater affinity to geographically distant Arab populations, than to their neighbours from the Indian subcontinent, who do not belong to an Islamic honorific lineage.     

The relationship of the Mimbres to other southwestern and Mexican populations

Previous aDNA research in the Southwest provides a framework to understand the Mimbres population which maintains a unique identity in the archeology of the desert Southwest of North America. Both the mitochondrial DNA (mtDNA) haplogroups and haplotypes of a sample of 46 Mimbres individuals were identified from skeletal remains and compared to those of other ancient and modern populations in the American Southwest and Mexico. The haplogroup frequency distribution of the Mimbres resembles that of other Southwestern populations, suggesting a close relationship between the Mimbres and the neighboring populations and population continuity for a long period of time. However, their relatively low frequency of haplogroup A (11% n = 5) is consistent with a clinal distribution of haplogroup A from Mesoamerica to the Southwest. Moreover, unlike other Southwestern populations, the Mimbres share haplotypes with populations to the south, such as the Cora, Huichol, and Nahua, suggesting gene-flow from Mesoamerica. Here we discuss this data in light of the origin of the Mimbres population and their relationship to other Southwestern and Mexican populations.     

Leprosy at the Lazaretto on St Eustatius,Netherlands Antilles

Leprosaria established in the Americas during the Colonial period bear many similarities with those found in medieval Europe. They are comparable in terms of isolation, the objectification of leprosy sufferers and their association with religious charities. The Lazaretto on St Eustatius was operated from 1866 to 1923. The site was investigated to recover palaeopathological evidence of leprosy at a leprosarium in the Americas. Five burials were excavated; three individuals showed evidence of bone modifications consistent with those caused by leprosy, including aspects of ‘rhinomaxillary syndrome’ and the bilaterally symmetrical post‐cranial changes that have been described in leprosy examples from medieval Europe. An exceptional find was the presence of potentially leprous bone changes to the hyoid, thyroid and 3^rd–6^th cervical vertebrae. Copyright © 2007 John Wiley & Sons, Ltd.     

Ancient DNA provides new insights into the origin of the Chinese domestic horse

Domestic horses played a pivotal role in ancient China, but their exact origin remains controversial. To investigate the origin of Chinese domestic horses, we analyzed mitochondrial DNA (mtDNA) from 35 horse remains, aged between 4000 and 2000 years, excavated from nine archaeological sites in northern China. The Chinese ancient horses exhibited high matrilineal diversity, falling into all the seven haplogroups (A–G) observed in modern horses. These results suggest that several maternal lines were introduced into the gene pool of Chinese horses in the past. Haplogroups A and F were more prevalent in ancient horses than the other haplogroups. Interestingly, only haplogroups A and F were present in the samples older than 4000 years, while the more recent horses (between 2000 and 3000 years BP) fell into all seven haplogroups. Comparison with DNA data of present-day horses suggests that haplogroup F is like to be an ancient haplogroup of East Asian origin. These analyses also suggest that the origin of Chinese domestic horses is complex, and external mtDNA input occurred after initial domestication. Our results indicate that the Chinese ancient horses are more related to the modern Mongolian horses. Lastly, our results cannot support the previous hypothesis that early Chinese domestic horses were derived from the Przewalski horse.     

Fossil dogs and wolves from Palaeolithic sites in Belgium,the Ukraine and Russia: osteometry,ancient DNA and stable isotopes

Using multivariate techniques, several skulls of fossil large canids from sites in Belgium, Ukraine and Russia were examined to look for possible evidence of the presence of Palaeolithic dogs. Reference groups constituted of prehistoric dogs, and recent wolves and dogs. The fossil large canid from Goyet (Belgium), dated at c. 31,700 BP is clearly different from the recent wolves, resembling most closely the prehistoric dogs. Thus it is identified as a Palaeolithic dog, suggesting that dog domestication had already started during the Aurignacian. The Epigravettian Mezin 5490 (Ukraine) and Mezhirich (Ukraine) skulls are also identified as being Palaeolithic dogs. Selected Belgian specimens were analyzed for mtDNA and stable isotopes. All fossil samples yielded unique DNA sequences, indicating that the ancient Belgian large canids carried a substantial amount of genetic diversity. Furthermore, there is little evidence for phylogeographic structure in the Pleistocene large canids, as they do not form a homogenous genetic group. Although considerable variation occurs in the fossil canid isotope signatures between sites, the Belgian fossil large canids preyed in general on horse and large bovids.     

Molecular and morphological analyses of avian eggshell excavated from a late thirteenth century earth oven

Using ancient DNA (aDNA) extracted from eggshell of the extinct moa (Aves: Dinornithiformes) we determined the species composition and number of eggs found in a late thirteenth century earth oven feature at Wairau Bar (South Island, New Zealand) – one of New Zealand’s most significant archaeological sites. Mitochondrial and nuclear DNA signatures confirmed this oven feature contained fragments of at least 31 moa eggs, representing three moa genera: Emeus; Euryapteryx; Dinornis. We demonstrate through the genetic identification of 127 moa eggshell fragments that thickness is an unreliable character for species assignment. We also present a protocol for assessing the preservation likelihood of DNA in burnt eggshell. This is useful because eggshell fragments found in archaeological contexts have often been thermally modified, and heat significantly increases DNA fragmentation. Eggshell is widely used in radiocarbon dating and stable isotope research, this study showcases how aDNA can also add to our knowledge of eggshell in both archaeological and palaeoecological contexts.     

Ancestral Puebloan mtDNA in context of the greater southwest

Ancient DNA (aDNA) was extracted from the human remains of seventy-three individuals from the Tommy and Mine Canyon sites (dated to PI-II and PIII, respectively), located on the B-Square Ranch in the Middle San Juan region of New Mexico. The mitochondrial DNA (mtDNA) haplogroups of 48 (65.7%) of these samples were identified, and their frequency distributions were compared with those of other prehistoric and modern populations from the Greater Southwest and Mexico. The haplogroup frequency distributions for the two sites were statistically significantly different from each other, with the Mine Canyon site exhibiting an unusually high frequency of haplogroup A for a Southwestern population, indicating the possible influence of migration or other evolutionary forces. However, both sites exhibited a relatively high frequency of haplogroup B, typical of Southwestern populations, suggesting continuity in the Southwest, as has been hypothesized by others (8, 9, 29, 45 and 57). The first hypervariable region of twenty-three individuals (31.5%) was also sequenced to confirm haplogroup assignments and compared with other sequences from the region. This comparison further strengthens the argument for population continuity in the Southwest without a detectable influence from Mesoamerica.     

Anthropological analysis of the osteological material from an ancient tomb (Early Bronze Age) from the middle Euphrates valley,Terqa (Syria)

Terqa, situated on the right bank of the Middle Euphrates, is known to have been a site already in the third and second millennium BC. Excavations which take place in this region aim to provide answers for numerous significant issues connected with the origins of human civilisation. In 2008 season we found a tomb dated 2650–2450 BC, consisting of two chambers with stone domes. The smaller chamber contained many luxury grave goods. The other one was bigger and contained human skeletons. The first skeleton belonged to a man, 45/50 years old. It is extremely heavy and large. On the right humerus, near the proximal edge, we found two cuts. The healed edges of the wound suggest that the man from Terqa survived after the wound was inflicted. Many muscular attachments were clearly marked on the bones and bone robustness was far above the average, which may suggest that the skeleton belonged to a warrior. These observations correspond to the fact that the bronze part of a belt together with bronze weapon‐blades was found on the right side of the hip. The second skeleton, which belonged to a female who was about 40/44 years old, was found in an anatomical position. The chamber also contained an almost complete skeleton of a sheep. The morphology of the forearm of the female suggested strenuous activity. From this skeleton was successfully isolated HVR1 fragment. The main mutation indicated that the analysed mtDNA belonged to haplogroup K. Copyright © 2010 John Wiley & Sons, Ltd.     

Rapid loss of endogenous DNA in pig bone buried in five different environments

The rate at which endogenous DNA from differently prepared (butchered, boiled and baked) compact pig bones degrades in five different Danish terrestrial and marine environments over 12 months was investigated. Although > 70% of the estimated endogenous mtDNA is lost after just four weeks of exposure, no cytosine deamination of DNA was recognised. A correlation between the presence of oxygen and the amount of preserved DNA was observed. The results provide valuable information on the interaction between the endogenous DNA and the depositional environment in the early stages of bone diagenesis, which can be a support in the interpretation of the initial diagenetic pathways of archaeological bone.     

A Probable Case of Thalassemia Intermedia from Tell Masaikh,Syria

In this report, we describe the first potential case of hereditary anaemia from the Middle Euphrates valley (Syria). The skeletal changes identified in the studied specimen were consistent with marrow hyperplasia, and the distribution, especially the involvement of the facial bones, the sternum and ribs, was suggestive of thalassemia. The identified haplotype indicates M4b1 haplogroup suggesting that the analysed specimen was either a newcomer from Asia or a descendent of Asian origin in the maternal line, most likely from the Indian Peninsula where characteristic mutations of mtDNA can be identified today. The genetic data might explain a case of thalassemia in the Syrian lower Euphrates valley. Copyright © 2015 John Wiley & Sons, Ltd.     

Determination of a kinship system using ancient DNA,mortuary practice,and historic records in an upper Canadian pioneer cemetery

Recovered and amplified ancient DNA (aDNA), from a historically documented 19th century Upper Canadian pioneer cemetery produced genotypes that were used to infer a past societal kinship system. While the results from multiplex short tandem repeat (STR) amplifications showed an unreliable polymerase chain reaction (PCR) product, a single locus HUMTH01 analysis yielded reproducible data and an allelic frequency pattern not statistically different from modern populations. Mitochondrial (mt) DNA HVR II data showed that a combined cemetery database exhibited reduced haplotype diversity indicators, as well as clusters of probable maternally related burials. The chronological persistence and replacement of mtDNA clusters approximately every two generations suggests a patrilineal/patrilocal kinship structure from a virilocal burial program for the Harmony Road cemetery. Through the integration of the aDNA analysis with archaeological material culture, historic records, and other ethnohistoric sources of information, this conclusion is supported. In this study persisting patrilineally inherited surnames act as a surrogate for aDNA Y‐chromosome haplotype analysis. These results suggest that aDNA applications on aggregate skeletal collections where sparse, or no ethnological or historical documentation exists, may result in incorrect population history inferences if the presence of a kinship interment bias is not considered. Copyright © 2003 John Wiley & Sons, Ltd.     

Ancient DNA insights from the Middle Neolithic in Germany

Genetic studies of Neolithic groups in central Europe have provided insights into the demographic processes that have occurred during the initial transition to agriculture as well as in later Neolithic contexts. While distinct genetic patterns between indigenous hunter-gatherers and Neolithic farmers in Europe have been observed, it is still under discussion how the genetic diversity changed during the 5,000-year span of the Neolithic period. In order to investigate genetic patterns after the earliest farming communities, we carried out an ancient mitochondrial DNA (mtDNA) analysis of 34 individuals from Wittmar, Germany representing three different Neolithic farming groups (ca. 5,200–4,300 cal bc) including Rössen societies. Ancient DNA analysis was successful for six individuals associated with the Middle Neolithic Rössen and observed haplotypes were assigned to mtDNA haplogroups H5, HV0, U5, and K. Our results offer perspectives on the genetic composition of individuals associated with the Rössen culture at Wittmar and permit insights into genetic landscapes in central Europe at a time when regional groups first emerged during the Middle Neolithic.