Stable isotope palaeodietary study of humans and fauna from the multi-period (Iron Age,Viking and Late Medieval) site of Newark Bay,Orkney

We report here on stable carbon and nitrogen isotope measurements of human and faunal bone collagen from the Iron Age, Viking Age, and Late Medieval site of Newark Bay, Orkney, Scotland. We found a wide range of results for humans in both δ¹³C (−15.4‰ to −20.3‰) and δ¹⁵N (8.6‰–15.6‰) values. The enriched carbon and nitrogen values indicate the consumption of significant amounts of marine protein, which is very unusual for post-Mesolithic (e.g. 4000 cal BC) UK and European populations. Also of interest is a statistically significant difference in δ¹³C (t = −2.48, p = 0.011) and δ¹⁵N (t = −2.44, p = 0.011) values, and therefore diets, between adult males and females at this site, with males (δ¹³C = −17.8 ± 1.2‰, δ¹⁵N = 13.2 ± 1.6‰) having, on average, a higher proportion of marine protein than females (δ¹³C = −18.9 ± 1.1‰, δ¹⁵N = 11.8 ± 1.8‰). The weaning age of the sub-adults was difficult to interpret due to the large isotopic variation in the adult females, but nearly all individuals between birth and 1.25 years have elevated δ¹⁵N values indicating that they were breastfed to some extent.     

An investigation of diet in early Anglo-Saxon England using carbon and nitrogen stable isotope analysis of human bone collagen

The principal aim of this work is to investigate whether protein sources in human diets in early Anglo-Saxon (5th-7th century AD) England varied with geographic location, or with respect to age or sex. The methodology used was analysis of δ¹⁵N and δ¹³C in human bone collagen from 76 adult skeletons from a total of 18 different cemeteries located in inland, riverine and coastal environments. In order to control for the possible effects of socio-economic status on diet, only high status individuals, as identified by grave goods, were studied. Data were analysed using statistical manipulation of the raw results and using IsoSource (Phillips and Gregg, 2003), a mathematical treatment which models the composition of the protein part of human diets using bone stable isotope ratios when the isotopic values for potential protein sources are known or can reasonably be inferred. The results suggest that protein sources varied little according to geographic location and that terrestrial foods dominated at all locations. However there were some subtle patterns. Slightly elevated δ¹³C in skeletons from coastal locations, and δ¹⁵N in those from riverine sites may indicate that communities in these locations made greater use of marine and freshwater resources respectively. If this is correct then it demonstrates that 5th-7th century communities made some use of wild resources even within a predominantly agrarian economy. In general the analysis of the raw data and the IsoSource results were mutually supportive, and this work demonstrates the value of this twin-pronged approach to analysis of human bone stable isotope data.     

Stable isotope evidence for palaeodiets in southern Turkmenistan during Historical period and Iron Age

The subsistence patterns of Iron Age and Historical period humans from south-western Turkmenistan have been reconstructed using the carbon and nitrogen isotopic compositions of archaeological faunal and human bones. A qualitative comparison of the isotopic signatures points to a small proportion of ruminant meat and dairy in human diet for both periods. The ranges of proportions of dietary items yielded by a quantitative approach based on concentration dependent mixing models confirm the high proportions of plant food in the average diet, and show little change in the reconstructed diet for both periods. A comparison of results from zooarchaeological and isotopic approaches illustrates their complementarity in subsistence patterns reconstruction.     

Cleft Lip Case in a Middle Bronze Age Young Man from Altai,Russia

Oral cleft (OC) is a common congenital anomaly in humans, which occurs in approximately one per 700 live births. However, cleft skulls are found relatively seldom in archaeological specimens, especially for the bc period. Mortality of infants with OC was apparently high because of breastfeeding difficulties and culturally sanctioned rejection of them in the past. This paper presents one of the most ancient cases of OC in Eurasia. The finding comes from the Middle Bronze Age pastoralists' cemetery in Altai, Russia. The skeletal remains have been radiocarbon dated to 1883–1665 cal bc . Cranial and dental measurements, computed tomography and radiography have been used to examine this malformation. The facial sculpting reconstruction has been performed to visualise the ante mortem appearance of the cleft face. Besides, δ¹³C and δ¹⁵N isotope analysis was used for a comparative study of the diet of the cleft individual. It has been established that a young man from Altai was affected by complete bilateral cleft lip and alveolus, which is a very rare form of typical OC. This defect is associated with dental anomalies such as hypodontia, microdontia, and ectopia as well as with dysmorphology of the facial complex outside the cleft area, including mild hypertelorism, hypoplasia of nasal bones and antheroposterior maxillary deficiency. Furthermore, aplasia of the frontal sinuses and the sacral spina bifida occulta have been observed. These findings are consistent with clinical data indicating strong correlation between development of a major defect and multiple minor anomalies. The results of the study also suggest that there was tolerant attitude towards the facial deformities among the ancient Altai pastoralists. Copyright © 2016 John Wiley & Sons, Ltd.     

A Bayesian C chronology of Early and Middle Bronze Age in Sicily. Towards an independent absolute dating

This paper proposes a Bayesian model for the ¹⁴C chronology of Sicilian Early and Middle Bronze Age, with a specific focus on the northeastern sector of the island. Building on the available ¹⁴C determinations, the model allows addressing a number of chronological questions left open in literature, making a first step towards an independent absolute chronology. The analysis put the start of the earlier part of Early Bronze Age (Capo Graziano 1-Casa Lopez phase) between 2400 and 2175 cal BC, and the end at about 1960 cal BC. The advanced stage of the same period (Capo Graziano 1-Filo Braccio phase) is likely to have started and ended around 1960 and 1730 cal BC respectively. The model indicates that the time slot with the highest posterior probability for the start of the Middle Bronze Age–Milazzese (Portella phase) is between 1490 and 1460 cal BC. This turns up to be earlier than held to date. Notably, the model enables for the first time to bracket the development of the later stage of Early Bronze (Capo Graziano 2-M. di Capo Graziano phase) between 1730 (end of Filo Braccio phase) and 1490/60 cal BC (start of Middle Bronze Age–Portella phase). The latter date is earlier than usually held for the end of Capo Graziano 2. The existence of a narrow gap between the end of the latter and the start of Middle Bronze Age-Portella phase is tentatively proposed only on stratigraphic grounds. Further, the analysis enables for the first time to pinpoint and quantify the lag that is likely to have existed between the start of those Sicilian prehistoric phases and the appearance of Late Helladic imports. The comparison with the Aegean ¹⁴C Bayesian chronology indicates that a time lag (about 45 yr) is likely to have occurred between the start of Capo Graziano 2 and of the Late Helladic period. A time lag between 20 and 70 yr is likely to have existed between the start of the Sicilian Middle Bronze Age and of the Late Helladic III. Arguments tentatively supporting the narrower interval are also discussed.     

Patterns of Resource Use and Isotopic Niche Overlap Among Guanaco (Lama guanicoe), Pampas Deer (Ozotoceros bezoarticus) and Marsh Deer (Blastocerus dichotomus) in the Pampas. Ecological,Paleoenvironmental and Archaeological Implications

ABSTRACT

In this paper, we analyze the isotopic niche and resource use of the guanaco (Lama guanicoe), pampas deer (Ozotoceros bezoarticus) and marsh deer (Blastocerus dichotomus) on the temperate plain of the northern Pampa region in the southeast of South America. The measured stable isotope compositions in bone tissue are δ¹³C, δ¹⁵N and δ¹⁸O. All the analyzed samples were recovered from different archaeological sites from the late Holocene. According to the results, the guanaco was strictly confined to the Pampa plain environment, developing a broad niche based on C₃ plants and a variable contribution of C₄ grasses within a grazing trend. The pampas deer preferentially used the Pampa plain, but also the prairies on the borders of the wetland, showing eurioic characteristics and a narrower niche based on C₃ plants. In turn, the marsh deer was strictly confined to wetland environments, developing a C₃ diet–base, within a narrow isotopic niche and stenoic characteristics. The three mammals showed a wide range of intraspecific variability, which was a key factor in their adaptability to spatial and temporal changes in the vegetation coverage. In fact, the temporal trends of their isotopic values were concurrent with the major climatic variations of the Holocene. Differences in the correlations between the values of both carbon sources in both deer species compared with the guanaco suggest a distinct chemical composition of their diet and/or differences in the allocation of nutrients. The isotopic values of nitrogen and spacing of the carbon sources in guanaco (pseudoruminant) and both deer species (ruminants) show no significant differences between them, thus establishing the values for local large herbivores. Significant correlations between δ¹³C and δ¹⁵N were found in the guanaco and marsh deer. The regional and extra–regional variability in the guanaco's δ¹³C_collagen and δ¹⁵N probably reflect the clinal variations in the vegetation coverage and the amount of rainfall. The collagen isotope values in the guanaco throughout the entire Holocene show that the humid Pampa would have shifted between being a more recurrent mesic and temperate plain with minor phases of dry–mesic conditions like during the Little Ice Age, and a humid and warm one at the peaks of the Holocene Thermal Maximum and the Medieval Climatic Anomaly. The guanaco and the pampas deer adapted to all the climatic changes that happened on the humid Pampa until the biological invasions of large European mammals changed the herbivores’ guild composition of this vast plain, pushing them into peripheral habitats due to competition. The within–species variability in isotopic signals through time and space make it necessary to carry out adequate sampling before reconstructing the diets of local past populations.     

Chemical,elemental, and isotopic effects of acid concentration and treatment duration on ancient bone collagen: an exploratory study

Demineralization of bone for collagen extraction is most commonly performed using dilute hydrochloric acid (HCl) despite the fact that little data exists on the effects of differing acid concentration or treatment time on the quantity and quality of collagen extracts and that hydrochloric acid is extremely effective at hydrolyzing peptide bonds. Data on yield, elemental composition, and isotopic signature from bone collagen obtained from multiple extractions performed on two large ancient human bone samples from prehistoric Puerto Rico indicate that there is no meaningful difference between acid treatments ranging from 0.05 to 0.2 M concentration and 24–120 h of treatment. As such, a shorter course of demineralization using more concentrated acid would appear to produce quality results in a shorter time.     

Stable isotope analysis of prehistoric populations from the cemeteries of the Middle and Lower Dnieper Basin,Ukraine

This paper presents the results of new stable isotopes (carbon and nitrogen) analysis of human, faunal and fish remains from thirteen cemeteries from the Middle and Lower Dnieper Basin, Ukraine. The results are integrated with earlier analyses, undertaken solely on human samples, in order to provide a comprehensive overview of subsistence across the Upper Palaeolithic through to Eneolithic periods in this region. This is the first time that a combined sample of human, faunal and fish remains has been studied in order to interpret subsistence strategies across these periods in Ukraine. The total dataset comprises 113 samples of human, faunal and fish remains, 59 of which include new analyses of faunal and fish remains that have not previously been made available for study (Table 1). The analysis of the faunal and fish remains allows for a consideration of trophic levels which indicates that the consumption of freshwater fish occurs from the Epipalaeolithic period onwards. Whilst the majority of the cemeteries and the individuals therein cluster in relatively tight groups, there is a significant offset between the human and faunal samples due to the consumption of freshwater resources. The fish samples analysed in the study appear to exhibit a relatively random distribution when compared to the other samples analysed. In general, freshwater resources remain significant through until the end of the Neolithic period and into the Eneolithic period at the sites investigated.