Stable isotopic analysis of human and faunal remains from the Incipient Chulmun (Neolithic) shell midden site of Ando Island,Korea

We analyzed stable carbon and nitrogen isotope ratios of human and animal remains from the Ando shell midden, South Korea. The Ando site is a rare Incipient Chulmun (Neolithic) site (ca. 6000–5000 BC), which contains well-preserved human and animal bones in shell mounds. The stable isotope results for humans (average δ¹³C = −13.5 ± 0.5‰ and δ¹⁵N = 15.2 ± 0.5‰) indicate that Ando people in the Incipient Chulmun period strongly depended on marine resources. There were no isotopic differences between humans of different sex and age at this site. We compared our data with other previous published isotopic data from the Chulmun sites and found that the Ando people had similar isotope values to the southern Chulmun people (Tongsamdong and Daepo), but different isotopic ratios than the western Chulmun people (Daejukri and Konamri). These results indicate that marine foods were the main food resources in the southern coastal regions, but not in the western coastal regions in Chulmun Korea.     

Oxygen isotope analysis of California mussel shells: seasonality and human sedentism at an 8,200-year-old shell midden on Santa Rosa Island,California

To evaluate coastal settlement and land use strategies among maritime hunter-gatherers, we analyzed oxygen isotope (δ¹⁸O) data from 131 marine carbonate samples from 21 California mussel (Mytilus californianus) shells obtained from a large ～8,200-year-old shell midden (CA-SRI-666) on California’s Santa Rosa Island. Seasonal distributions of the isotopic data were assigned using a paleo-sea surface temperature model created by comparing modern sea surface temperatures (SST) to a fully profiled ～8,200-year-old shell. For 20 additional shells, we used two sampling strategies to compare season-of-harvest inferences and explore whether the Early Holocene site occupants were sedentary. Estimated season-of-harvest differed by 35 % between the two sampling methods, corroborating recent isotope analysis of an 8,800-year-old shell midden on San Miguel Island. Shellfish appear to have been collected year-round at CA-SRI-666 from intertidal or subtidal water temperatures similar to modern SST in the vicinity of eastern Santa Rosa Island. The isotope results are consistent with other evidence from CA-SRI-666 that suggest that the site served as a residential base for relatively sedentary maritime people.     

Subsistence and mobility strategies in the Epipalaeolithic: a stable isotope analysis of human and faunal remains at 'Uyun al-Hammam,northern Jordan

This paper investigates evidence for subsistence and settlement activities in the Levantine Middle Epipalaeolithic through the application of stable isotope analysis to human and non-human remains from the site of 'Uyun al-Hammam, northern Jordan. In general, bone from the site suffers a high degree of diagenesis and collagen could not be extracted for analysis here. Carbon and oxygen isotopic values from human tooth enamel samples were variable, but within expected values for the Middle Epipalaeolithic, whereas animal carbon and oxygen isotopic values varied widely, most likely due to hydrological and climatological factors. Carbon and isotopic values for the human samples indicated a predominantly C₃ plant dietary input, while animal samples appeared to have varying amounts of C₄ inputs into their diet. This is the first isotopic analysis conducted on material from the Middle Epipalaeolithic of the southern Levant and, as such, even results constrained by temporal and climatological variants within the region contribute to the overall knowledge of settlement and subsistence strategies during this cultural period.     

Paleodietary analysis of a San Francisco Bay Area shellmound: stable carbon and nitrogen isotope analysis of late Holocene humans from the Ellis Landing site (CA-CCO-295)

The late Holocene archaeofaunal record of the San Francisco Bay Area demonstrates temporal declines in the abundance of low-cost, high-ranked marine and terrestrial resources. During later periods of occupation, faunal assemblages are often dominated by lower-ranked, higher-cost resources, suggesting an increase in diet breadth through time. Archaeological resource intensification models argue that this marks a late Holocene decline in foraging efficiency in the Bay Area, driven by human-induced harvest pressure. This study examines dietary change in the region using stable carbon and nitrogen isotope data from 65 human burials, spanning two temporal components (cal AD 55–890 and cal AD 762–1550) at the Ellis Landing site (CA-CCO-295). The strong linear relationship between collagen carbon and nitrogen isotope values reflects both marine and terrestrial food consumption, with individuals showing a high level of dietary variability at the site. No temporal trend or meaningful sex differences were found in isotope values. The widening of diet breadth predicted by the archaeofaunal record occurred prior to the occupation of the Ellis Landing shellmound site. The trend in exploiting a greater amount of terrestrial resources during the Middle and Late Period in central California was supported by isotopic evidence.     

Stable Isotopic Analysis of Human Skeletons from the Sunhung Mural Tomb,Yeongju, Korea: Implication for Human Diet in the Three Kingdoms Period

This study reports the results of stable isotope analyses (carbon, nitrogen and sulphur) of human bone collagen from the Sunhung mural tomb from the Three Kingdoms Period of Korea (AD 300–668). The stable isotope data indicate that the main source of protein in the diet of the interred seven Sunhung individuals came from C₃‐based terrestrial resources, and there was an isotopic variation between individuals at this site. To investigate dietary patterns in the Three Kingdoms period, we compared our results with reported isotopic data from other southeastern Three Kingdoms sites (Imdang‐dong, Songhyeon‐dong and Yean‐ri). We found that the Sunhung individuals had similar isotope ratios to the people from these other sites. However, there was noticeable isotopic difference among individuals from each study site, although much of the dietary protein in each site was mainly from terrestrial sources. We propose that the most parsimonious explanation for this isotopic pattern is variation in social status during this time period. Copyright © 2012 John Wiley & Sons, Ltd.     

Carbon and nitrogen stable isotope analysis on the diet of Jomon populations from two coastal regions of Japan

We report on a stable isotope paleodietary reconstruction of Jomon populations in Japan during the Middle to Final Jomon period (ca. 5000–2300 years BP), focusing on dietary differences within and among populations and between regions. Carbon and nitrogen isotope analysis was performed on human and faunal bone collagen from six coastal sites along the Inland Sea in the Sanyo (Ota, Funamoto, and Tsukumo) region and along Mikawa Bay and the Pacific Ocean in the Tokai (Kawaji, Yoshigo, and Inariyama) region. We found that carbon and nitrogen isotope ratios were positively correlated, indicating that the Jomon people consumed a mixed diet of marine (shellfish and marine fish) and terrestrial (C₃ plants and terrestrial mammals) protein. In the Ota samples (n = 25, during the Middle Jomon period, 5000–4000 years BP), sex was one of the main reasons for the intra-population dietary variation. Ota males consumed greater amounts of marine food, while Ota females consumed greater amounts of terrestrial food; these dissimilar diets may have been related to the sexual division of labor. Significant inter-population dietary differences were found, which may have been related to differences in age or site location. Notably, the two coastal regions showed clear isotopic differences. Nitrogen isotope ratios of individuals from the Sanyo region were significantly higher than ratios of individuals from the Tokai region. The individuals in the Sanyo region might have consumed a diet high in aquatic foods, particularly high trophic level marine fish, whereas the individuals in the Tokai region might have consumed a lot of marine shellfish. Another possible reason for the regional isotopic difference might have been different baseline of nitrogen isotope ratios of the marine ecosystems.     

Seasonal Patterns of Coastal Exploitation on the Farasan Islands,Saudi Arabia

Here we present the results of the analysis of coastal exploitation patterns in the southern Red Sea during the Middle Holocene. We focus on the shell midden cluster of the Farasan Islands, Saudi Arabia, which comprises over 3,000 shell midden sites. These sites date from 6,500 to 4,500 cal BP and are part of an arid landscape. We focus on one site, JW1727, which provides a snapshot of marine exploitation and will help to understand the use of food resources within the region. Stable isotope values (δ¹⁸O) were collected from the marine gastropod Conomurex fasciatus (Born 1778), which represents 72% of shell weight of JW1727, in order to reconstruct the season of capture. Results demonstrate that 1) every season is represented within the dataset; and 2) there is increased C. fasciatus deposition during the summer and autumn months. This indicates a diet consisting of C. fasciatus throughout the year in combination with other food sources and an increase of the C. fasciatus component during the arid seasons, possibly linked to the unavailability of vegetation. Additionally, size measurements of C. fasciatus were carried out to examine changes in size distribution throughout the occupation of the site that could be related to overexploitation of C. fasciatus. However, no significant trends could be observed. In sum, the results suggest a sustainable and constant habitation of the Farasan Islands despite the highly arid conditions.     

Neolithic fish remains from the freshwater shell midden Riņņukalns in northern Latvia

The Neolithic site Ri??ukalns in the Lake Burtnieks/River Salaca area in northern Latvia is the only freshwater shell midden in the eastern Baltic Sea area. An excavation carried out in 2011 revealed an intact stratigraphy with alternating layers of unburnt and burnt mussel shells and yielded various kinds of archaeological finds, among them several thousand fish remains. To gain an understanding of the fish species and specimens caught by the Neolithic settlers, and to discern any temporal development in the fish species composition, we analysed fish remains from different sections and layers. Results from both the archaeozoological and stable isotope data, give evidence for a change in the relevance of fish species during the period of use, and they also provide information for reconstructing the former river and lake hydrology in the vicinity of the midden. The Stone Age landscape seems to have been very similar to the present situation, so that the study area has been an extraordinarily stable ecosystem for more than 5000 years. Comparisons with the results of recent monitoring programmes, long-term changes since Medieval times, and written sources from the 18th century, show that the fish species community is almost unchanged since the Stone Age. This underlines the importance of the region in nature conservation.     

Radiocarbon Marine Reservoir Effect in Human Remains from the Kitakogane Site,Hokkaido, Japan

Accelerator mass spectrometry (AMS) was used to measure radiocarbon (¹⁴C) in bones excavated from the Late Jomon shell midden at the Kitakogane site, Hokkaido, Japan. Comparison between ¹⁴C ages of terrestrial and marine mammals from the same site showed systematic age differences which could be attributed to the ¹⁴C marine reservoir effect in the western North Pacific Ocean. Furthermore, this effect was clearly observed in human remains from this site, indicating a heavy dependence on seafood. Dietary habit was estimated from δ¹³C and δ¹⁵N measured in the same human remains. Apparent ¹⁴C ages indicated 79% of protein originated from marine sources.     

Strontium isotope evidence of migration and diet in relation to ritual tooth ablation: a case study from the Inariyama Jomon site,Japan

Ritual tooth ablation was extensively practiced among Jomon (Japanese Neolithic) societies in their final phase (ca. 3000-2300 BP). This tradition includes two different tooth ablation patterns, type 4I and type 2C, referring to extraction of the mandibular incisors and canines, respectively. However, the reason for this difference is unclear. Previous carbon and nitrogen stable isotope analysis of human remains from the Inariyama shell mound revealed that type 4I individuals were more dependent on terrestrial resources and type 2C individuals on marine resources. To test this hypothesis, we performed strontium (Sr) isotope (⁸⁷Sr/⁸⁶Sr) analyses on the same skeletal remains and on modern plants around the site. Because Sr isotope ratios of plants differ according to the local geology and seawater has a consistent Sr isotope ratio, the Sr isotope ratios of tooth enamel can reveal both migration and diet. Comparing Sr isotope ratios in plants and seawater with those of tooth enamel, we identified four possible immigrants. Type 4I locals had significantly higher Sr isotope ratios than type 2C locals. The ratios of the type 4I and type 2C locals were close to those of terrestrial plants and seawater, respectively, suggesting that type 4I locals had incorporated much Sr from terrestrial resources and type 2C locals from marine resources. These results support the hypothesis that ritual tooth ablation reflects dietary differences throughout an individual’s life, and they suggest possible occupational differentiation among the Jomon people.     

Possible Wild Boar Management during the Ertebølle Period. A Carbon and Nitrogen Isotope Analysis of Mesolithic Wild Boar from Fannerup F,Denmark

This paper presents a stable isotope and radiocarbon study on a total of 85 samples of wild boar (Sus scrofa), humans (Homo sapiens), dogs (Canis familiaris), red deer (Cervus elaphus) and roe deer (Capreolus capreolus) from four Late Mesolithic sites in Jutland, Denmark. Four of the eight Sus scrofa samples from one site, the shell midden of Fannerup F, show markedly enriched carbon and nitrogen isotope values, indicating a dietary intake of a substantial amount of marine food. In contrast to standard interpretations of Late Mesolithic animal economy, we suggest that the enriched values of Sus scrofa may be an indication of management by Ertebølle groups in the area that facilitated access to substantial amounts of marine foods for these wild boars compared to contemporaneous conspecifics. The ¹⁴C dates of the Sus scrofa range from 5290 to 4335 cal BC, suggesting that the management of Sus scrofa developed independently of contact with Neolithic societies. Although the sample size remains small, the interpretation of Late Mesolithic animal management adds to the growing evidence for political and economic complexity in the Ertebølle culture.     

Harvesting the Seashores in the Late Mesolithic of Northwestern Europe: A View From Brittany

The shell middens of Brittany provide the last evidence of a Mesolithic way of life along the French Atlantic façade. This is partly a result of Holocene marine transgressions that prevent easy access to earlier coastal settlements. Nevertheless, the dependence on the sea seen in the Late Mesolithic seems to be a consequence of a long-established exploitation system. Stable carbon and nitrogen isotope signatures in human bone reflect a dominance of marine protein, while the zooarchaeological components of shell middens show a high species richness of exploited marine resources. Paleoenvironmental reconstruction suggests that more or less the whole range of resources exploited was accessible in the immediate vicinity of the sites. Seasonal aspects of the utilised and potentially available subsistence resources, along with stable isotope and lithic data, raise the possibility of restricted mobility for these populations, within relatively limited territories. The impression of extreme dependence of these coastal populations on the seashore might have been a key factor in their final disappearance, whether this is viewed as replacement or acculturation. Indeed, the Mesolithic communities of Brittany could have been caught between rising sea-levels and the arrival of Neolithic communities from the east and the south.     

Fast or feast: reconstructing diet in later medieval England by stable isotope analysis

In this pilot-study, which was designed to assess the range of isotopic variation in English medieval populations, we present the results of stable isotope analysis of carbon and nitrogen of human and animal bone collagen from three later medieval sites in Northern England.The isotopic values observed for the rural hospital of St. Giles by Brompton Bridge (N. Yorks.), the Augustinian Friary at Warrington and a mass-grave with casualties from the Battle of Towton (N. Yorks.) are significantly different from those reported for other archaeological populations in Britain, namely by their very enriched δ¹⁵N ratios which are combined with almost entirely terrestrial carbon signals. We discuss possible explanations for the unusual human data and argue on grounds of the available faunal data, that a mixed diet of terrestrial, marine and freshwater resources is most likely. This may indicate the significant impact of the medieval fasting regulations on everyday subsistence. We conclude that stable isotope analysis can complement the available historical information on diet in the Middle Ages.     

ON THE ROAD TO PARADIS: NEW INSIGHTS FROM AMS DATES AND STABLE ISOTOPES AT LE DÉHUS,GUERNSEY, AND THE CHANNEL ISLANDS MIDDLE NEOLITHIC

We here report the results of a programme of AMS dating and stable isotope analysis on human remains from the chambered tomb of Le Déhus, Guernsey. An early use‐phase in the range 4100–3900 BC is indicated, confirming the monument's attribution to the Middle Neolithic II as defined in western France. Late Neolithic burial activity is also identified. Stable carbon isotope measurements provide little or no evidence for the consumption of marine foods, although stable nitrogen isotope values are unusually high. These results are situated in the wider context of Neolithic mortuary monuments of the Channel Islands and Normandy.     

Opportunistic versus target mode: Prey choice changes in central-western Korean prehistory

Human decision-making processes are usually hierarchical in that higher-level decisions impose constraints on lower-level decisions. As a result, prey choices during individual foraging trips are governed to a large degree by higher-level decisions regarding how to supply resources to satisfy demands, with higher-level decisions typically made prior to foraging trips. Resource selectivity and search bias sometimes take place in this context. By dividing resource procurement modes into opportunistic and target, I discuss how choice of mode on the basis of an overall economic plan affects prey choice during foraging trips and faunal assemblage composition resulting from those trips. An analysis of taxonomic diversity in shellmidden assemblages from the central-western Korean Late Chulmun Period (3500–1300 BC) and Middle and Late Mumun Period (700–100 BC) shows that Late Chulmun people adopted a target mode, while Middle/Late Mumun people adopted an opportunistic mode in their exploitation of marine resources. A decrease in the importance of marine resource in Middle/Late Mumun produced a change in taxonomic diversity by increasing the opportunistic cost of marine resource exploitation.     

Stable carbon and nitrogen isotope analysis on human remains from the Early Mesolithic site of La Vergne (Charente-Maritime,France)

We report here the results of stable carbon and nitrogen isotope analysis of human and faunal remains from La Vergne (Charente-Maritime, western France), a rare Early Mesolithic burial site (ca. 8500–8000 cal BC). The results for nine humans (average δ¹³C = −19.3‰; δ¹⁵N = 9.4‰) indicate a strongly terrestrial diet, dominated by animal protein, with the possibility of, at best, a slight contribution of marine-derived protein. Given lower sea-levels in the early Holocene, the site would have been some 60–80 km from the sea at the time of its use; nevertheless, contacts with the coast are shown by the presence of numerous marine shell beads in the graves. In the light of the stable isotope results, it is suggested here that such contacts most likely took the form of exchange with coastal communities whose remains now lie underwater.     

Stable Isotopic Evidence of Ancient Maya Diet at Caledonia,Cayo District,Belize

The skeletal remains of 18 individuals interred at the ancient Maya site of Caledonia (100 to 1000 C.E.), located in the Cayo District of Belize, w ere sampled for stable carbon and nitrogen isotope analysis in order to reconstruct their diet. Stable carbon and nitrogen isotope ratios in 18 bone collagen samples and stable carbon isotope ratios in bioapatite from 15 bone and 6 tooth enamel samples were assessed. Small sample sizes precluded the assessment of dietary variation with respect to age, sex, social status or time period among the Caledonia Maya. However, the sampled individuals consumed a varied diet consisting of maize, supplemented with some C₃ plants, terrestrial herbivores and/or lower order freshwater resources such as snails and molluscs and possibly maize‐fed animals. This dietary variability with an emphasis on maize is unsurprising given the biological diversity surrounding the site and the known importance of this crop to the ancient Maya. As expected, the isotopic values from Caledonia are similar to those from nearby sites from similar time periods. However, four individuals exhibit a marine dietary signature, possibly indicating inland trade of marine resources from coastal sites, or the migration of coastal people to Caledonia. This study demonstrates the validity of sampling small, fragmented collections from minor Maya centres in order to gain valuable insight into ancient Maya dietary practices. Copyright © 2013 John Wiley & Sons, Ltd.     

Late Mesolithic burials at Casa Corona (Villena,Spain): direct radiocarbon and palaeodietary evidence of the last forager populations in Eastern Iberia

Current knowledge about the Mesolithic–Neolithic transition in the Central and Western Mediterranean European regions is deeply limited by the paucity of Late Mesolithic human osteological data and the presence of chronological gaps covering several centuries between the last foragers and the first archaeological evidence of farming peoples. In this work, we present new data to fill these gaps. We provide direct AMS radiocarbon dating and carbon (δ¹³C) and nitrogen (δ¹⁵N) stable isotope analysis were carried out on bone collagen samples of two single burials from the recently discovered open-air Late Mesolithic site of Casa Corona (Villena, Spain). The results shed new light on the chronology and subsistence patterns of the last Mesolithic communities in the Central Mediterranean region of the Iberian Peninsula. Radiocarbon results date the human remains and funerary activity of the site to 6059–5849 cal BC, statistically different from other Late Mesolithic sites and the earliest Neolithic contexts, and bridging the 500 yrs chronological gap of the Mesolithic–Neolithic transition from the area. Isotopic evidence shows that diet was based on terrestrial resources despite the proximity to the site of lagoon and marine ecosystems. This and previous isotope studies from the region suggest a lower reliance upon marine resources than for Atlantic and Cantabrian sites, although intra-regional patterns of neighbouring Mesolithic populations exhibit both fully terrestrial diets and diets with significant amounts of aquatic resources in them. We hypothesize that in the Central Mediterranean region of Spain the Late Mesolithic dietary adaptations imposed structural limits on demographic growth of the last foragers and favoured rapid assimilation by the earliest Neolithic populations.     

The earliest evidence of domesticated wheat in the Crimea at Chalcolithic Ardych-Burun

Abstract

This paper reports the discovery of the earliest evidence of domesticated wheat in the Crimean peninsula from the Ardych-Burun shell midden site, Ukraine. The Ardych-Burun site dates to middle of the 4th millennium cal b.c. For the first time, the chronology of a Ukrainian Chalcolithic period site has been established through direct radiocarbon dating of cereal grains retrieved from it. This discovery allows for a wider discussion of the chronology and geographical origins of domesticated plant species in Ukraine and the role the Caucasian corridor may have played in the spread of agriculture into eastern Europe. The presence of cereal crops in the southern Crimea enriches our understanding of the subsistence strategies of the coastal population, which was previously linked only with pastoralism, hunting, and the exploitation of marine resources.     

Inter-site variability in the season of shellfish collection on the central coast of British Columbia

High-resolution stable oxygen isotope analysis of the bivalve Saxidomus gigantea from shell midden sites was applied to identify seasonal patterns of resource procurement on the central coast of British Columbia, Canada. A total of 90 archaeological shells were examined from eight distinct sites spanning a 4500-year period. Combining micro-growth pattern analysis with high-resolution stable oxygen isotope sampling allows for a precise season of collection to be determined in estuarine bivalves recovered from archaeological sites. The results of the stable oxygen isotope analysis provide insights into seasonally structured harvest of S. gigantea (butter clam), which is associated with different types of sites. The results show a variety of patterns, including multi-seasonal collection, intensive seasonal harvesting and casual, supplemental use of butter clams at different locations.