A case of multiple metastasis in Late Holocene hunter‐gatherers from the Argentine Pampean region

Chenque I site is a prehistoric cemetery located in Lihué Calel National Park (La Pampa province) in the Western Pampean region of Argentina. Hunter‐gatherer societies made use of this site during the Final Late Holocene for at least 700 years (1030–370 BP). Currently 41 burial structures have been excavated, and more than 150 individuals have been recovered. There is great variability in mortuary patterns at the site (simple, multiple, primary, secondary burials, and also a variant not previously observed in the region). The life‐ways of this population have been investigated through the evaluation of several biological and cultural factors. Several pathological conditions have also been identified in this cemetery. Burial no. 12 contains a skeleton of an adult male that shows multiple pathological lesions, compatible with a neoplastic disease. These lesions have been analysed using several methodological strategies: macroscopic, radiological and microscopic. This is the first time that this kind of disease has been identified from a prehistoric burial in Argentina. In this paper the location and characteristics of the lesions are evaluated, and the different neoplastic diseases that could have produced them are discussed. Since the people buried in this cemetery belonged to highly mobile societies, a key issue is to infer the consequences that this disease would have had on the dynamics of the group in which this person lived, because of the gradual deterioration of his health and physical strength. Copyright © 2007 John Wiley & Sons, Ltd.     

Earliest evidence of spinal tuberculosis from the Aneolithic Yayoi period in Japan

Tuberculosis has existed from early prehistoric days to modern times. The main causative agents of tuberculosis worldwide are Mycobacterium tuberculosis (M. tuberculosis) and M. bovis, along with M. africanum, M. cenettii and M. microti; these species make up the ‘M. tuberculosis complex’. This worldwide infection has been of special interest to palaeopathologists due to its characteristic bone lesions as well as its great antiquity. Historically, tuberculosis has been recognised in Japan for more than a thousand years. However, the origin and early prevalence of tuberculosis remain unknown. In the present study, we present the earliest evidence of skeletal tuberculosis found in the Aneolithic Yayoi period in Japan (ca. 300 BC to AD 300). The skeletal remains showing typical pathological changes of spinal tuberculosis were dated to between 454 BC and AD 124 by dendrochronological methods using coburied arrow-shield board and house columns made of Japanese cedar. We discuss the early prevalence of this infectious disease and its influence on the population history of the Japanese from prehistoric to Aneolithic times. Copyright © 2006 John Wiley & Sons, Ltd.     

Possible cases of leprosy and tuberculosis in medieval Sigtuna,Sweden

In Sigtuna, Sweden, a medieval cemetery, including 227 skeletons, was analysed in 2006. On the outskirts of the churchyard, six skeletons with bone changes indicating systemic inflammatory disease were observed. Two out of three individuals with well‐preserved facial bone regions displayed signs of rhinomaxillary remodelling. Four of the afflicted exhibit severe bilateral alterations of the lower legs and phalanges of the feet and concentric atrophy of the metatarsals. In addition, one of the individuals exhibited a kyphosis in the lumbar vertebrae. In a discussion about alternative diagnoses, lepromatous leprosy and tuberculosis were identified as the causes of the destructive lesions in two individuals. Though the skeletal changes of the lower legs and feet in four cases demonstrate a close resemblance to secondary lesions of leprosy, the disease could not be confirmed. The skeletal changes of the last individual were unspecific and the possible causes several, rendering diagnosis difficult. The burial locations imply that the afflicted persons belonged to a lower social stratum. Due to the significantly higher frequency of pathological changes in the cemetery compared to other cemeteries in the town, the individuals could be regarded as fellow sufferers among others with various medical conditions. The bioarchaeological identification of systemic infectious diseases of a group of individuals of this size is unique to north of Scania in Sweden, where only a few cases of leprosy and tuberculosis have previously been diagnosed. The significance of the present study is emphasised by the interconnection between the afflicted, the archaeological context and the knowledge of the medieval society in Sigtuna. Copyright © 2010 John Wiley & Sons, Ltd.     

A Bobcat Burial and Other Reported Intentional Animal Burials from Illinois Hopewell Mounds

Abstract

The Elizabeth site is a bluff-top mortuary mound group constructed and primarily used during Hopewellian (Middle Woodland) times. Recent reanalysis of nonhuman skeletal remains from the site reveals that an intentional burial previously identified as a dog (Canis familiaris) is actually an immature bobcat (Lynx rufus). As a result of this discovery, we reevaluated eight other purported animal burials from Illinois Middle Woodland mounds, including seven dogs and a roseate spoonbill (Platalea ajaja). The dogs all appear to be intrusive or unrelated burial events, but both the bobcat and the roseate spoonbill were definite Hopewellian mortuary interments. The roseate spoonbill was decapitated and placed beside a double human burial. But the bobcat was a separate, human-like interment wearing a necklace of shell beads and effigy bear canine teeth (Figures and ). To our knowledge, this is the only decorated wild cat burial in the archaeological record. It provides compelling evidence for a complex relationship between felids and humans in the prehistoric Americas, including possible taming.     

A Fisk patent metallic burial case from Western Missouri: an interdisciplinary and comprehensive effort to reconstruct the history of an early settler of Lexington,Missouri

In 2006 a cast-iron coffin was discovered in an unmarked burial plot in Lexington, Missouri. A multifaceted investigation was conducted to provide historical documentation and possible identification of the individual. The coffin is an early Fisk Patent Metallic Burial Case. Osteological analyses indicate that the skeletal remains belong to a 20 to 30 year old white female who consistently ate an omnivorous diet with significant amounts of C4 plants or seafood. Rib morphology and her burial garments suggest she frequently wore restrictive clothing. No gross skeletal pathological lesions or trauma were observed except for a patch of reactive bone and an atypical pattern of bone remodeling on the visceral surface of the sixth rib. Subsequent bacterial DNA analysis of the ribs and sternum indicate the presence of tuberculosis infection. Although not conclusive, multiple lines of evidence are consistent with the skeletal remains representing Elizabeth (Triplett) Stewart who died in 1854 of pulmonary tuberculosis. This multidisciplinary research significantly contributes to the local history of Lexington, Missouri and provides a likely identification of the deceased individual for the Stewart Family.     

The Meul Index: A non‐destructive method for estimating the cortical index in skeletal samples

The study of osteoporosis in past populations provides insight into the history and evolution of this condition. The Cortical Index is a relative measure of cortical bone often used for identifying individuals with age‐related bone loss associated with osteoporosis. Current methods of measuring the Cortical Index involve radiography or cutting actual sections of long bones at the mid‐shaft. We propose a new index, the Meul Index as means for estimating the Cortical Index without the use of radiography. Based on a preliminary study of 13 individuals consisting of 9 males, 3 females and one unknown sex, ranging in age from young to older adult, the Meul Index is highly correlated with the Cortical Index (r = 0.903). The proposed Meul Index provides a non‐destructive and inexpensive means for studying age‐related bone loss in prehistoric and historic populations represented by skeletal samples. Our research builds on the recent publication of Silva, A.M., Crubezy, E. and Cunha, E. 2008. Bone weight: new reference values based on a modern Portuguese identified skeletal collection. Int. J. Osteoarchaeol. DOI: 10.1002/oa.998. Copyright © 2009 John Wiley & Sons, Ltd.     

Stable Isotope Analysis of Diet‐based Social Differentiation at Late Prehistoric Collective Burials in South‐Western Portugal

In the Neolithic and Copper Age collective burials of the Portuguese Estremadura, the majority of material culture and skeletal remains are highly commingled, making it difficult for archaeologists to evaluate social status by linking individuals with specific grave goods. In these circumstances, bio‐anthropological data about individual life histories offer an additional avenue of investigation into social complexity among prehistoric communities practising collective burial. In this study, stable isotope data were gathered from 81 individuals from seven collective burial sites and one settlement, the fortified site of Zambujal, in order to determine if significant dietary differences exist within or between burials that may point to patterns of social differentiation, both at individual sites and across the region. While in general all of the sampled individuals consumed fairly homogeneous diets based on terrestrial animal proteins and C₃ plants, this study found that statistically significant differences in δ¹³C_ap and δ¹⁵N values exist between several sites, which may indicate socially differentiated consumption of meat and plants. Additionally, statistically significant differences were found in δ¹⁵N values between adults and juveniles, which may either be attributable to protein‐restrictive child‐feeding practices or physiological processes related to skeletal growth and development.     

The Living and The Dead,Memory and Transition: Bayesian Modelling of Mesolithic and Neolithic Deposits from Vlasac,the Danube Gorges

The paper applies Bayesian statistical modelling to radiocarbon dates obtained for a stratigraphic sequence comprising occupation features and superimposed burials from the Late Mesolithic (c.7400–6200 cal BC) to the Mesolithic–Neolithic transition (c.6200–5900 cal BC), from Vlasac in the Danube Gorges region of the north‐central Balkans. This sequence, investigated in the course of excavations at the site in 2006–9, yielded stratigraphic evidence of the transformation of local forager populations as a result of contact with Neolithic communities. Our paper provides a reliable chronological framework for changes from Late Mesolithic burial rites to new, Neolithic types of ornamental beads at the top of the sequence. The use of the same burial location and continuities in burial rites over a considerable period of time raise significant questions about the role of tradition and the potential for enduring practices in prehistoric societies.     

On the accuracy of estimating living stature from skeletal length in the grave and by linear regression

This study evaluates a method for obtaining stature estimates for populations represented by skeletal material, with individuals buried in a supine position. During the excavation of a Danish mediaeval cemetery, in situ skeletal length in the grave was measured from a point above the cranial point farthest from the body to the most distal point of the talus. The measurement was made with a folding rule placed on the sagittal midline of the skeleton, allowed to follow any curvature of the skeleton in situ. In the laboratory, stature was reconstructed anatomically, and this stature was regarded as an accurate estimate of living stature. Stature was also reconstructed from femur length by two linear regression procedures: 1) by sample and sex specific formulae, employing a leave‐one‐out approach, and 2) by sex wise formulae for Euro‐Americans from Trotter & Gleser (1952, American Journal of Physical Anthropology 10 : 463–514). Skeletal length in the grave and the two stature estimates based on linear regression were compared to anatomically reconstructed stature. Skeletal length in the grave estimated anatomically reconstructed stature with practically no bias (95% CI: −1.3–1.5 cm). Sample specific regression formulae estimated anatomically reconstructed stature also with no bias (95% CI: −1.2–1.1 cm). In contrast, statures calculated from Trotter & Gleser's regression formulae estimated anatomically reconstructed stature with a bias of about 4 cm (95% CI: 3.3–5.0 cm). Estimates of stature variance were biased for all three estimation procedures. However, for samples of adults, an adjusted variance estimate can be obtained by subtracting 8.7 cm² from the variance obtained from skeletal lengths in the grave. It is recommended to measure skeletal length in the grave whenever possible, and use this measurement for estimating statures for prehistoric and early historic populations. Copyright © 2005 John Wiley & Sons, Ltd.     

Tuberculosis on the north coast of Peru: skeletal and molecular paleopathology of late pre-Hispanic and postcontact mycobacterial disease

This paper examines skeletal and ancient DNA evidence in the study of suspected tuberculosis infection in the late pre-Hispanic and Colonial-era Lambayeque Valley Complex, north coast Peru (A.D. 900–1750). We integrate information on macroscopic lesion characteristics and distribution, radiographic and CT scan imagery, and analysis of Mycobacterium tuberculosis complex rpoB and IS6110 ancient DNA (aDNA) sequences. Destructive lesions were observed in the vertebral bodies of three precontact indigenous adult males, one colonial adolescent female, and in the cranium of a Colonial-period subadult. Assessment of lesion morphology and distribution led us to consider multiple diseases, but tuberculosis represents the most likely diagnostic option in all individuals. DNA was poorly preserved in all samples, but an IS6110 sequence was amplified in one precontact individual consistent with macroscopic diagnosis. These findings expand the geographic and temporal extent of tuberculosis to the late pre-Hispanic and Colonial north coast of Peru to highlight potential synergisms between diet, settlement patterns, and the evolution of Andean tuberculosis before and after European conquest. Moreover, this study helps focus several key questions in Andean tuberculosis research, including possible reassessment of the presence of the IS6110 sequence in the pre-Columbian Americas. Methodological considerations include differential diagnosis – especially with incomplete skeletons – and limitations of aDNA studies underscoring an approach integrating macroscopic, radiographic, and molecular lines of evidence in the paleopathological investigation of one of humankind’s most devastating and destabilizing diseases.     

Selenium in skeletal remains

Contents of selenium (Se) were determined in human skeletal remains of prehistoric populations by in situ trapping of Se hydride by ET AAS (atomic absorption spectrometry with electrothermal atomisation). Dr Korunová worked out a method of determination of Se in preparation. The method of determination of Se was verified by means of radioactive indicator ⁷⁵Se incorporated in the tissues of laboratory animals. Detection limit of the method was 23 pg Se. Se is another element suitable for dietary reconstruction in past populations as it relates to the consumption of meat in a similar way to zinc. Through the analysis of Se, we were able to distinguish between Eneolithic archaeological cultures (Corded Ware ceramic, Bell Beaker culture) and Bronze Age cultures (Protounětice, Starounětice, Unětice cultures). Significant differences were found in the levels of Se in the bones of individuals derived from the Bell Beaker and Uneětice Cultures, to the 95% confidence interval. Copyright © 2010 John Wiley & Sons, Ltd.     

Comparative Study of Perimortem Weapon Trauma in Two Early Medieval Skeletal Populations (AD 400–1200) from Ireland

Violence was a reality of life in early medieval Ireland (AD 400–1200). Its omnipresence is indicated from numerous narratives of regicide, mortal conflicts, battles and warfare that survive in ancient myths, legends and annalistic accounts. The archaeological evidence of violence and conflict is mainly identified in the osteoarchaeological record, and approximately 13% of all skeletal populations from excavated early medieval cemeteries in Ireland have shown evidence of weapon trauma. This study considers the osteological representation of violent deaths in two contemporaneous Irish skeletal populations dating to this period: Mount Gamble in County Dublin and Owenbristy in County Galway. This analysis involves assessing the different anatomical regions of the body for evidence of lesions that can be attributed to weapon trauma. The results indicate that these populations are likely to have been exposed to violence under differing circumstances; the evidence suggests that the individuals from Mount Gamble may have been well equipped or skilled at interpersonal battle, in contrast to the majority of individuals from Owenbristy who may have been unprotected and unprepared. The presence of two adolescents and two adult females amongst the victims from the latter population gives insight into a wider social dimension of weapon trauma in early medieval Ireland. There is also evidence of postmortem mutilations and decapitations, which reflect ritualistic aspects of violence. Copyright © 2012 John Wiley & Sons, Ltd.     

Eneolithic Cattle Burials from Aljmaš‐Podunavlje and Osijek‐Retfala,Croatia

Two single bovine burials and one mixed animal burial (containing bovine and canid skeletal remains) have been unearthed at two Baden culture sites, Aljmaš‐Podunavlje and Osijek‐Retfala located in Eastern Croatia. Zooarchaeological analysis attributed the faunal remains to domesticated cattle (Bos taurus) and in one case a dog (Canis familiaris). Almost complete and articulated skeletons of subadult or adult cows were found in all three examined features, while pit 59/60 from Aljmaš also contained a skull belonging to an adult‐domesticated cattle and a skeleton of a very young dog, 2–3 months old. All of the skeletons are well preserved and display no evidence of carcass processing indicating that the animals were buried intentionally. Consideration of the positions in which these animals were interred and their relationship with nearby deposits enables discussion of potential animal burial strategies and possible connections with certain ritual practices in which cows and in one case a dog played important roles. The described burials represent the southernmost known distribution of the rite discussed. Copyright © 2015 John Wiley & Sons, Ltd.     

Palaeoepidemiological Patterns of Trauma in a Medieval Nubian Skeletal Population

Evidence of trauma was investigated in a well-preserved skeletal sample from the Medieval Sudanese Nubian site of Kulubnarti. The skeletal materials derive from two temporally over-lapping Christian cemeteries, dating from the sixth to circa the sixteenth century. The available sample consisted of the skeletons of 146 adults which were investigated for fractures of the long bones, crania and the hands and feet, as well as for dislocations and muscle pulls. Results showed a high incidence of long bone fractures, seen especially in the forearm, and involving 33.5 per cent of individuals. Many of these lesions indicated quite severe injury, and an unusually high number of affected individuals (27 per cent) showed multiple long bone involvement. Conversely, only one possible cranial fracture was apparent. Fractures were also found in 13 hand and seven foot elements. In addition, one hip dislocation and evidence of pulled tendons in 11 individuals were also observed. Compared to other similarly controlled samples, the Kulubnarti population stands out for its high prevalence of healed fractures, the high proportion of multiple involvement and the severity of numerous lesions. The forbiddingly harsh and uneven terrain of this region of Nubia was most likely a major influence on the unusually high prevalence and pattern of traumatic lesions in this group. © 1997 by John Wiley & Sons, Ltd. Int. J. Osteoarchaeol., 7 : 103–114 (1997) No. of Figures: 5. No. of Tables: 5. No. of References: 25.     

Weapon‐related Cranial Lesions from Medieval and Renaissance Turin,Italy

Archaeological excavations carried out in the square around the Cathedral of S. Giovanni in Turin brought to light burials referable to the medieval and Renaissance periods. The anthropological examination of the skeletal remains allowed to identify two skeletons from the medieval period (10th–11th centuries) and four skeletons from the Renaissance age (15th century) showing weapon‐related cranial injuries. These peri mortem lesions are indicators of interpersonal aggression and in particular of armed conflicts. The two individuals from the early medieval period presented three traumas consisting in sharp force lesions caused by bladed weapons. As regards the Renaissance sample, the majority of the nine peri mortem injuries were sharp force wounds, followed by a blunt force trauma. These distribution patterns might reflect different fighting techniques, whereas the side distribution and location of the skull trauma provide further indications on the fighting modalities. Identification of the weapons that caused these traumas is suggested. The lack of post‐cranial wounds at Piazza S. Giovanni might be explained by the greater attention paid to the head, which was the main target of attack, or by adequate protection of the body through medieval and Renaissance armours. Otherwise, the wounds in the body would have been found only in the soft tissues, with no involvement of the bones. Despite the presence of weapon injuries, the results obtained from the study of the Renaissance sample are different from the findings of other contemporary battlefields. It is highly likely that the individuals of the Renaissance age were not young soldiers employed in war episodes and brought back for burial in Turin after battles that had taken place elsewhere. Instead, they were probably individuals who had died in riots or in other violent city episodes, as the historical records for the Renaissance age seem to confirm. Copyright © 2013 John Wiley & Sons, Ltd.