Osteometric and molecular sexing of cattle metapodia

Sex identification of skeletal remains based on morphology is a common practice in Zooarchaeology. Knowledge of the sex distribution of slaughtered or hunted animals may help in the interpretation of e.g. hunting or breeding strategies. Here we investigate and evaluate several osteometric criteria used to assess sex of cattle (Bos taurus) metapodia using molecular sex identification as a control of the metric data. The bone assemblage used to assess these new criteria derives from the Eketorp ringfort in the southern parts of Öland Island in Sweden. One hundred metapodia were selected for molecular analysis of sex and we were able to genetically identify the sex of 76 of these elements. The combined results of the molecular and osteometric analyses confirm a significant size difference between females and males for several measurements for both metacarpals (Mc) and metatarsals (Mt). Our results show that some measurements are applicable for metapodials. These measurements include the slenderness indices such as the Mennerich’s index 1 and 3, as well as the distal breadth (Bd), the breadth between the articular crests (Bcr), and the maximum breadth of the lateral trochlea (BFdl). We show that they can be used for sexing of both metacarpals and metatarsals. The latter measurements offer an opportunity to study fragmented elements and thus a higher number of elements may be utilized for morphological sexing of archaeological bones. Size comparisons of Mc and Mt may also aid in the separation of bulls and oxen.     

Accurate sex identification of ancient human remains using DNA shotgun sequencing

Accurate identification of the biological sex of ancient remains is vital for critically testing hypotheses about social structure in prehistoric societies. However, morphological methods are imprecise for juvenile individuals and fragmentary remains, and molecular methods that rely on particular sex-specific marker loci such as the amelogenin gene suffer from allelic dropout and sensitivity to modern contamination. Analyzing shotgun sequencing data from 14 present-day humans of known biological sex and 16 ancient individuals from a time span of 100 to ∼70,000 years ago, we show that even relatively sparse shotgun sequencing (about 100,000 human sequences) can be used to reliably identify chromosomal sex simply by considering the ratio of sequences aligning to the X and Y chromosomes, and highlight two examples where the genetic assignments indicate morphological misassignment. Furthermore, we show that accurate sex identification of highly degraded remains can be performed in the presence of substantial amounts of present-day contamination by utilizing the signature of cytosine deamination, a characteristic feature of ancient DNA.     

A new approach to sexual diagnosis in past populations. Practical adjustments from Van Vark's procedure

Determination of sex is a key aspect in the study of past populations. Bias in skeletal sexing is well known, however, and depends upon the completeness of the skeletal remains and the representativeness of reference samples. In order to correct these difficulties, the authors propose practical adjustments to Van Vark's well‐known procedure. Two fundamental aspects are considered to reduce the bias of sex allocation. First, we propose to increase the effectiveness of sexing during the first step (primary diagnosis only from the pelvic bone). Second, to improve the final step (secondary diagnosis from the extra‐pelvic skeleton), we consider posterior probabilities as essential. These two practical adjustments are successfully tested in a sample of known sex and lead to a classification with 94.5% accuracy. These results significantly improve sex determination in archaeological samples and therefore contribute to a better understanding of past populations. Copyright © 1999 John Wiley & Sons, Ltd.     

Osteology,DNA and Sex Identification: Morphological and Molecular Sex Identifications of Five Neolithic Individuals from Ajvide,Gotland

Sex identifications are crucial for the understanding of the societies we study. To understand an ancient society we need to have some picture of the roles played by males and females in the society. This knowledge has often been produced by morphological sex identifications in connection with grave goods and burial finds. However, if a morphological sex identification is to be fully trusted, the human remains need to be fairly well preserved. There is also a problem in the fact that morphological traits identified in a modern material are applied on an ancient material. There is a growing difference with time and geographical distance. An alternative to morphological identification is molecular identification based on the presence or absence of a Y chromosome, but such identification has its problems too: the risk of contamination and the fact that the absence of proof is not a good female indicator. In this work we have tried to compare morphological and molecular identifications of five Neolithic individuals from the Ajvide site at Gotland in the Baltic sea. © 1997 by John Wiley & Sons, Ltd.     

The conundrum of castration in the archaeological record: An interpretation of Roman cattle horn-cores from Chelmsford,Essex

Current methods of sexing archaeological cattle bones, in particular the metapodials, are based upon past research into contemporary breeds and this work embraces a limited sample size, spread across a vast geographical area. Although the separation of cows and bulls is not in question, there appears a great deal of subjectivity in the literature concerning the identification of castrated beasts. Living bone is in a dynamic state of equilibrium with the rest of the body and responds to increased loading, that is, an increase in weight of the beast or stress/strain through strenuous movement, by remodelling. If cows and oxen are performing the same work, as for example in ploughing, then loading on the bones will be similar. These bones will thus respond by remodelling in corresponding fashion, and hence any morphological and metrical distinctions will be clouded. Horn-cores are less subject to these constraints, although in some cultures there may be loading on these bones, for example through attaching the harness to the horns for ploughing. The Chelmsford cattle horn-cores constitute waste from some industrial activity and it is argued in the text that horn-working is the most likely candidate, together with possibly tanning. Sexing of the bones strongly indicated male animals, with few females being present. A procedure for detecting castrated cattle (oxen) is presented, the results of which suggest an equivalent ratio of oxen to bulls.     

Severe vertebral collapse in a juvenile from the graveyard (13th/14th–19th centuries) of the São Miguel church (Castelo Branco,Portugal): differential palaeopathological diagnosis

Diseases that culminate into vertebral collapse are of intricate diagnosis both in palaeopathology and modern clinical practice. When analysing human skeletal remains from the archaeological record this difficulty is amplified due to the absence of complementary medical diagnostic information. This is especially evident when the distinction between tuberculous and pyogenic spondylitis is intended. Taking into consideration this challenging task and based on the macroscopic and radiological study of the skeleton number 8, a specimen exhumed from the East necropolis (13th/14th to 19th centuries) of the São Miguel church, at the Portuguese city of Castelo Branco, the aims of the work here presented are to discuss the range of possible aetiological factors, especially infectious ones, ascribable to the striking pathological changes noticed on this 12‐year‐old individual. These included alterations on the axial skeleton, namely extensive vertebral destruction, presenting as a gibbus deformity, and correlated thoracic deformities. Consubstantiated on palaeopathological and clinical research, tuberculous spondylitis seems to be the most probable cause for the reported lesions. However, the scrutiny between this condition and other pyogenic spinal infections is of extreme complexity when analysing ancient human remains and deserves in‐depth future investigations. Within the framework of the Portuguese archaeological record, the specimen here presented is of major relevance since the pattern and severity of the spinal osseous changes observed were not previously reported. Further, if tuberculous spondylitis is assumed as the most probable diagnosis, the case here presented represents one of the earliest skeletal evidence of this condition in Portugal. Copyright © 2009 John Wiley & Sons, Ltd.     

Ancestry Estimation Based on Morphoscopic Traits in a Sample of African Slaves from Lagos,Portugal (15th–17th Centuries)

In 2009, a skeletal collection of 158 individuals was excavated in Valle da Gafaria, Lagos, Portugal. These individuals were buried in an unusual way, having been discarded in an urban dump located outside the medieval city walls, dated from the 15th–17th centuries. Lagos was, at the time, an important slave trade harbour, and during the excavation, the morphological appearance of the skulls and the presence of intentionally modified teeth in some individuals raised suspicion that they were African slaves. Despite the extensive historical information about the Atlantic slave trade, so far, skeletal remains identified as slaves were scarce, especially in Europe. The aim of the present study is to estimate the ancestry of a sample of 33 adult individuals (28 women and 5 men) recovered in the Valle da Gafaria applying eleven morphological characteristics using the naïve Bayes classifier. When comparing the individuals with four groups of classification (European, African, American Indian and Asian), 24 (72.7%) specimens were classified as Africans with a posterior probability greater than 0.90. When only two groups were considered (the African and the European), 31 (93.9%) individuals were classified as Africans with a posterior probability greater than 0.90. These results are in accordance with the historical record and previous genetic studies, suggesting that this sample represents a rare archaeological sample of great interest to the history of the Atlantic slave trade, that is, the Lagos individuals were probably of African ancestry. Although the ancestry is a parameter of the biological profile mainly estimated in forensic Anthropology, this study confirms the importance of its investigation in past populations. Copyright © 2016 John Wiley & Sons, Ltd.     

Mitochondrial DNA studies of the Pazyryk people (4th to 3rd centuries BC) from northwestern Mongolia

The discovery and excavations in 2006 by joint Russian–German–Mongolian expeditions of the Pazyryk culture burial sites (4th to 3rd centuries BC, Early Iron Age, the Scythian period) in the Altai mountains of northwestern Mongolia near the Russia border provided new material for studying various aspects of these ancient peoples lives, including human, animal and plant remains. Ice accumulation in the graves preserved the human remains, allowing biological analysis of the samples. We conducted a genetic study based on mitochondrial DNA from remains of three Pazyryk culture representatives to investigate the possible genetic relationships of this Siberian Scythian group with populations of adjacent territories. These data support possible genetic contacts between populations of Altai and other Eurasia regions in the Early Iron Age, and are in good agreement with corresponding archaeological and anthropological data. However, a large-scale study of the Pazyryk population gene pool structure must be performed to further confirm these findings.     

COMPUTED TOMOGRAPHY AND COMPUTED RADIOGRAPHY OF LATE BRONZE AGE CREMATION URNS FROM DENMARK: AN INTERDISCIPLINARY ATTEMPT TO DEVELOP METHODS APPLIED IN BIOARCHAEOLOGICAL CREMATION RESEARCH

To improve the methods used to study prehistoric cremation rituals, cremation urns from the Danish Late Bronze Age were examined using computed tomography and computed radiography (digital X‐ray). During micro‐excavation, the digital images were used as a registration tool. Our results suggest that osteological ageing and sexing are more accurate when combining CT images with excavated remains. Digital volume rendering further enables a compromised estimation of the original cremation weight. Micro‐excavation is clearly a primary cause of bone fragmentation. Cremated remains affected by lower cremation intensity show markedly poorer preservation and recovery than do white calcined fragments. Thus post‐excavation estimations of cremation intensity are systematically biased.     

Loading and stability of a late 16th century Portuguese Indiaman

The stability characteristics of 16th century ships are not known with certainty, but safety issues related to floatability, stability and overloading were a cause of concern at the time. The aim of the paper is to advance knowledge in this field by developing a set of loading conditions for a typical Portuguese ship of this epoch, for both the voyage from Lisbon to India and the return voyage. This allows testing the reconstruction of the presumable Nossa Senhora dos Mártires as well as to use this reconstruction to bring a better understanding of safety and loading issues on the Portuguese East India route. Given the uncertainties about the loading conditions, several hypotheses are tested, varying the amount of ballast, the degree of overloading and the distribution of weights on board, and allowing the development of a range of plausible loading arrangements. The stability of the ship is then assessed using modern tools to develop the limit KG curve for compliance with a modern stability criterion applicable to large sailing vessels. The case study ship is a plausible reconstruction based on the analysis of nautical archaeological remains, contemporary documents and the use of modern naval architecture methods.     

Radio – Ulnar Weight Index — is it a Useful Criterion for Sex Identification of the Human Skeleton?

Sex identification of human long bones has been studied by several investigators. Measurements of the radius and the ulna have also been reported as an indicator of sex by reference to separate parameters and/or to combinations of the parameters of a single bone. This study was designed to determine whether the radius and the ulna can provide reliable information for the sexing of human bones, as can the lower leg bones, if the forearm measurements include the weight index of the two bones as a parameter. The materials consisted of the dried radii and ulnae of 20 recently deceased Japanese males and 20 similar females. Almost all the dimensions exhibited statistically significant sex differences. The radio–ulnar weight index did not, however, differ between the sexes. In discriminant analysis, the rate of correct sex discrimination based on distances between various pairs of points was not improved by the inclusion of the radio–ulnar weight index. Therefore, the radio–ulnar weight index cannot be recommended as a parameter for the sexing of human bones.     

Limited yet informative: pathological alterations observed on human skeletal remains from third and second millennia bc collective burials in the United Arab Emirates

While syntheses of palaeopathological studies have been undertaken in many parts of the world, until recently little was known about human health in the past in the United Arab Emirates (UAE). Focusing on skeletal pathologies, this paper documents the results of the first synthetic palaeopathologlcal study for this country. The collections of archaeological human skeletal remains come from three sites dating to the third and two sites dating to the second millennia bc and consist predominantly of disarticulated, partially fragmented and often cremated remains. The paper initially discusses potential explanations for the neglected study of human skeletal remains from the UAE. This discussion is followed by an outline of the types of burial practices employed in the past in this region and their effects on preservation. The nature of the collections and the methodology employed in their study are described. Using specific headings, the pathological findings are then documented, followed by a discussion of the results of the skeletal analysis in light of the available archaeological evidence. Finally, it is shown that although limited by preservation, analyses of human skeletal remains provide valuable details about the types of diseases that affected people in the past in the UAE. While less detailed than some palaeopathological syntheses, the results presented in this paper lay the foundations for further work on humans in the past in eastern Arabia. Copyright © 2001 John Wiley & Sons, Ltd.     

Using microscopy to improve a diagnosis: an isolated case of tuberculosis‐induced hypertrophic osteopathy in archaeological dog remains

The use of histology to estimate age, understand taphonomic history and diagnose disease in human skeletal and mummified remains is a well‐known method. Even though non‐human animals experience stress and disease similar to humans, microscopy is rarely applied to faunal remains to obtain a diagnosis. Histological results from a 16^th‐century Iroquoian dog which had previously been diagnosed by macroscopic and molecular methods with tuberculosis‐induced hypertrophic osteopathy (HPO) are presented here. Comparisons indicate that canid microscopic skeletal manifestations of HPO are reminiscent of human alterations caused by this condition. In addition, the microscopy reveals an aggressive form of HPO that was chronic in nature which could not be identified by either macroscopic or molecular methods. By providing an extension to the original diagnosis, the impact of a chronic case of tuberculosis is discussed. This study helps to emphasise the utility of palaeohistopathology in both biological anthropology and zooarchaeology, as it allows for a deeper discussion of the manifestation of HPO and the impact of tuberculosis on both dogs and humans. Copyright © 2008 John Wiley & Sons, Ltd.     

Prevalence rates of spondylolysis in British skeletal populations

As an activity‐related pathological lesion, spondylolysis and its prevalence rates are indicative of relative diachronic activity levels in different populations. In this paper we document the prevalence of spondylolytic defects in a series of time‐successive populations with special reference to the recording methods employed, and compare the findings with modern clinical studies. We identify epidemiological trends in expression of the condition through 1500 years in a series of skeletonised human remains from England. This includes a 5th–6th‐century settlement, a 15th‐century mass grave, a 14th to 17th‐century rural parish, a medieval Dominican friary, a medieval leper hospital and an 18th to 19th‐century crypt collection. These skeletal populations sample human groups experiencing considerable social change from an agrarian, non‐centralised early medieval period through the development of the medieval state to the earliest phases of industrialisation in England. A detailed study of all lumbar vertebrae in one of the assemblages highlights discrepancies between clinical prevalence rates for spondylolysis established through radiography, and those resulting from direct osteological analysis of the lumbar region of the vertebral column. Current prevalence rates cited in the osteological as well as the clinical literature are greatly dependent upon the recording methods employed, and the effects of several methods for osteological remains are considered in this treatment. For the populations reported on here, prevalence rates vary from considerably less than 1% to as much as 12%, depending on the method selected. A standardised recording method for spondylolytic lesions is suggested to facilitate accurate prevalence reporting and comparison of activity levels between different populations. Copyright © 2005 John Wiley & Sons, Ltd.     

Sexual dimorphism in limb bones of ibex (Capra ibex L.): mixture analysis applied to modern and fossil data

Estimating sex ratios of fossil bone assemblages is an important step in the determination of demographic profiles, which are essential for understanding the palaeobiology and palaeoethology of any particular species, as well as its exploitation patterns by humans. This is especially true for ibex (Capra ibex), which was a main source of food for hominids during Pleistocene times. Classical methods for determining sexual dimorphism and sex ratio, such as analyses using uni‐ and bivariate plots, are based on an arbitrary fixing of limits between sexes. Here we use a more robust statistical method termed mixture analysis (MA) to determine the sex of postcranial remains (long bones, metapodials and tarsals) from ibex. For the first time, we apply MA to both a modern and a fossil sample of one species, by using metric data taken from (i) a collection of present‐day ibex skeletons and (ii) a Palaeolithic sample of the same species. Our results clearly show that the forelimb (humerus and radius) is more dimorphic than the hindlimb (femur and tibia) and is therefore better suited for sexing ibex. It also appears that metapodials should be used carefully for estimating sex ratios. On the basis of these results, we propose a classification of bone measurements that are more or less reliable for sexing ibex. The results of MA applied to the ibex fossil bones from the Upper Palaeolithic site of the Observatoire (Monaco) lead us to the conclusion that this assemblage consists of a majority of males. The quantitative estimations calculated by the MA make it possible to compare the size of Pleistocene and modern ibex for the whole set of variables used in this study. Copyright © 2006 John Wiley & Sons, Ltd.     

Isotopes as palaeoeconomic indicators: new applications in archaeoentomology

Previous studies have demonstrated the potential of archaeologically-recovered insect remains to stand as evidence of past human activities such as trade and industry. The present investigation reports the recovery of stable-isotopes from modern and Neolithic insect fauna and explores their utility in addressing issues of palaeoeconomic significance. While in its infancy, the application of stable-isotope methods towards archaeological insects may provide insight into exchange patterns and land-usage in the past. In the present analysis, stable-isotopes from modern and archaeological insects showed potential as a means of assessing local versus non-local signatures. Further study is recommended.     

Cribra orbitalia and porotic hyperostosis in a Greek colonial population (5th to 3rd centuries BC) from the Black Sea

Cribra orbitalia and porotic hyperostosis in Greek skeletal remains have typically been attributed to a genetic anaemia, most notably thalassemia due to the presence of endemic malaria in the Mediterranean region. This paper reports the results of an analysis of cribra orbitalia and porotic hyperostosis in a Greek colonial population (5th to 3rd centuries BC) from the Black Sea. Archaeological evidence, stable isotopic data, and other skeletal indicators of physiological stress are examined to determine the most likely cause(s) of these lesions. While the possibility that some of the colonists of Apollonia suffered from thalassemia cannot be entirely discounted, the skeletal evidence examined in this study is not consistent with a diagnosis of thalassemia. We must therefore be cautious in assuming that cribra orbitalia and porotic hyperostosis in Greek skeletal remains, and indeed skeletal remains from any region where malaria was endemic in the past, is always indicative of this condition. Nutritional deficiencies and exposure to infectious diseases probably played an equally, if not more, important role in the development of these lesions. Copyright © 2006 John Wiley & Sons, Ltd.     

A Palaeopathological Assessment of the Late 19th and Early 20th Century Khoesan

Since the arrival of Dutch colonists in the Cape of Good Hope, South Africa, Khoesan populations were subjected to severe political and economic marginalisation and often fell prey to racial conflict. These circumstances persisted until the early 20th century, during which an astonishing number of Khoesan skeletons were transported from South Africa to various locations in Europe, as at the time, different institutions competed to obtain these remains. The purpose of this study was to assess the health status of the late 19th and early 20th century Khoesan. Skeletal remains housed in two different European institutions were studied. The sample comprised 140 specimens from the Rudolf Pöch Skeletal Collection in Vienna, Austria, and 15 specimens from the Musée de l'Homme in Paris, France. These individuals represent both sexes and were aged between newborn and 75 years, with 54 being younger than 20 years of age. Results indicated high levels of typical disease conditions associated with groups under stress, such as periostitis, cribra orbitalia and porotic hyperostosis. Treponemal disease, rickets, osteoarthritis and trauma were also encountered amongst other more specific indicators of health and disease. This study provided additional knowledge on the health status and lives of the Khoesan people during this turbulent period and created new awareness regarding a group of severely mistreated individuals. Copyright © 2014 John Wiley & Sons, Ltd.     

The Relationship Between Linear Enamel Hypoplasia and Social Status in 18th to 19th Century Edo,Japan

Linear enamel hypoplasia (LEH) has been utilised in human bioarchaeology as an indicator of growth disturbance during childhood. However, only a few studies have compared populations of different socio‐economic status in the same time period. This study examines the association between the LEH occurrence pattern and social status in the 18th to 19th century populations in Japan. Detailed archaeological studies on burials from the Edo period (1603–1867) excavated in Tokyo have facilitated estimating an individual's social status by its burial type. In this study, 112 individuals from the Sugenji‐Shokenji site were divided into three burial structure groups (ceramic jar coffins for middle‐class warriors, wooden square coffins for low‐class warriors and townspeople and wooden circular coffins for townspeople) and examined for the general prevalence of LEH, number of LEH and the chronological distribution of LEH. A high general prevalence of LEH was observed in every group, especially in lower canine (79.3–100.0%). However, individuals in the jar coffin group showed a lower prevalence and smaller number of LEH per tooth, suggesting that individuals of higher social status experienced better living environments in their childhood. There was no significant difference in the chronological distribution of LEH formation between coffin groups. Such data are essential for understanding the association between socio‐economic status and living conditions in specific societies in the past. Copyright © 2015 John Wiley & Sons, Ltd.