Stable isotope evidence of human diet at the Nukdo shell midden site,South Korea

Carbon and nitrogen stable isotope analysis was undertaken on bone collagen extracted from archaeological human (n = 48) and animal (n = 45) skeletons from the Nukdo site, Location I C, South Korea. This shell midden and grave site is dated from the late Mumun (550–300 BC) to early Iron Age (300 BC-1 AD) periods. The herbivorous mammals fell within the range of C₃ consumers, with average values of δ¹³C = −21.0 ± 0.5‰ and δ¹⁵N = 3.6 ± 0.5‰ for deer (n = 16) and δ¹³C = −20.6 ± 0.5‰ and δ¹⁵N = 4.5 ± 2.0‰ for wild boar (n = 17). Humans from this site averaged δ¹³C = −18.3 ± 0.4‰ and δ¹⁵N = 11.2 ± 0.7‰ for adults (n = 15) and δ¹³C = −18.7 ± 0.7‰ and δ¹⁵N = 12.5 ± 1.1‰ for juveniles (n = 33). These δ¹³C values indicate that there was no significant input of C₄ plants in the human diets and this may be associated with the spread of rice agriculture in the Mumun period. Human bone collagen δ¹³C and δ¹⁵N values indicate that there was some consumption of marine foods, although the main protein sources were from terrestrial foods. The isotope data demonstrate that the humans at Nukdo had mixed diets that included marine and terrestrial protein, including C₃ plants such as rice. Finally, the isotope results from the juveniles indicate that weaning occurred before the age of 1.5 years in this period.     

Stable isotopes,diet, and taphonomy: a look at using isotope-based dietary reconstructions to infer differential survivorship in zooarchaeological assemblages

Archaeology has always faced the problem of making informed inferences based on an incomplete record. Zooarchaeological studies of prehistoric hunting and diet offer a clear case in point, where a range of behavioral and taphonomic factors can produce a substantial disconnect between what people actually captured and ate and what archaeologists recover and interpret. We explore this disconnect by presenting stable C and N data for wild faunas, archaeological maize, and three human burials from Fremont-period sites in southeastern Utah, the United States. We use these data to estimate faunal contributions to prehistoric diets and compare the results with previous zooarchaeological analyses of faunas from the same sites. Results for the two approaches differ sharply, with isotopic estimates showing much higher contributions of small and lowland game. We discuss these results in terms of both local prehistory and wider issues of taphonomy and dietary analysis.     

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Fifty-eight dental calculus samples from medieval and post-medieval skeletons from Vitoria, Spain, and a single sample from an Alaskan Inuit were tested for stable carbon and nitrogen isotope compositions. There was sufficient carbon and nitrogen concentrations to obtain δ¹³C and δ¹⁵N values, and the samples from Spain produced results that were replicable and comparable to European isotope values based on bone collagen collected from literature sources. The Alaskan Inuit calculus sample yielded a δ¹⁵N value of +17.5‰, well beyond the range of the Spanish samples, but consistent with literature data for modern Greenlandic Inuit consuming a diet rich in marine food. There are several potential sources for carbon and nitrogen in calculus. The results of this study yield stable isotope values consistent with those obtained from other biomaterials used as isotope proxies for paleodietary research, including bone collagen, hair, and fingernails, although further work is necessary to verify the fidelity of calculus as an isotope proxy. Many studies in bioarchaeology are precluded by curatorial concerns regarding the destructive analysis of primary biomaterials. However, calculus is an “add-on”, or secondary biomaterial, that is not an integral part of the dental or skeletal system. Hence, its consumption during analysis is technically not destructive. Therefore, isotope analysis of dental calculus may provide a potential new avenue for paleodietary analysis where the use of other primary biomaterials is precluded.     

Stable hydrogen isotopes of bone collagen in palaeodietary and palaeoenvironmental reconstruction

The stable hydrogen isotope ratios (δD) of bone collagen in archaeological human and animal samples demonstrate a trophic level effect, with increasing δD from herbivores to omnivores to humans, in steps of 10–30‰. In addition the archaeological sites studied (Yarnton, Eton Rowing Lake, Danebury Environs–Suddern Farm, and Windmill Hill in the UK, Balatonszárszó in Hungary, and Huari in Peru) demonstrate geographical variation in δD. The detection of manuring in prehistory by comparison of δ¹⁵N to δD values in humans and a local herbivore (cattle) is also considered. This work is the first to measure δD in a large number and range of archaeological samples, with several animal species and humans. It demonstrates unequivocally that δD is different between species in ancient material, increasing from herbivores to omnivores to carnivores.     

Stable isotopes and diet at Ancient Kerma,Upper Nubia (Sudan)

Carbon and nitrogen stable isotope values were measured from bone collagen extracted from archaeological Nubian human (n = 54) and faunal (n = 61) populations from the site of Kerma, Sudan. Collagen suitable for isotopic analysis was extracted from 22 faunal and 48 human samples from the Eastern cemetery site, dated to the Middle Kerma (c. 2050–1750 BC) and Classic Kerma (c. 1750–1500 BC) periods respectively. The isotopic data indicate that the human dietary regimen included a mix of C₃ and C₄ plant-derived components, with a larger C₄ component than previously reported in archaeological Egyptian Nile Valley populations. Elevated δ¹⁵N values are attributed to consumption of dietary resources from a ¹⁵N-enriched terrestrial ecosystem. The faunal isotope data also indicate the consumption of C₃ and C₄ plants. The large range of δ¹³C values measured in both the human and faunal samples supports previous work suggesting that a significant portion of the populations buried at Kerma may have originated elsewhere, further confirming the Nile Valley as a major corridor for population movement in the region.     

Stable isotopic evidence for diet in a Roman and Late Roman population from Leptiminus,Tunisia

This study investigates the diet of the Roman and Late Roman population of Leptiminus on the Mediterranean coast of Tunisia. Stable carbon and nitrogen isotope analysis of human bone collagen and carbonate samples obtained from individuals buried in four cemeteries at Leptiminus was conducted in order to reconstruct the diet of the population, investigate the relative importance of marine vs. terrestrial resources, explore sex-, age-, and status-based variations in diet, examine temporal changes in the types of foods consumed, and compare the diet at Leptiminus with that of other Roman populations. The results of this study indicate that the residents of Leptiminus consumed a diet that was heavily reliant on terrestrial plant resources with the addition of a significant amount of marine resources. There were no significant sex differences in isotope values. In contrast, distinct dietary differences were seen between the adults and children. Nitrogen isotope values suggest that weaning began before the age of two and was completed by about 3 years of age, a finding consistent with previous isotopic studies of Roman samples. A temporal shift in diet is suggested by the nitrogen isotope values measured in samples from the most recent cemetery. A comparison of the data from Leptiminus with that derived from other Roman sites indicates that regional variability in diet existed within the Empire.     

Stable isotopic evidence of diet in a Greek colonial population from the Black Sea

Archaeological and literary sources indicate that the ancient Greeks relied heavily on terrestrial resources and that their access to certain types of foods varied by sex and status. Human and faunal remains from the Greek colonial site of Apollonia (5th to 2nd century BC) on the Black Sea coast of Bulgaria were analyzed for stable carbon and nitrogen isotopes in order to reconstruct the diet of this population, investigate the relative importance of marine vs. terrestrial resources, and explore variations in diet with respect to age, sex, and burial type. The results of this study indicate that the colonists of Apollonia relied on a mixed diet of terrestrial and marine foods, and that there was little or no variation in diet by age, sex, or burial type. The relationship between δ¹³C and δ¹⁵N data for these samples suggests that while marine foods were an important source of nitrogen, much of the carbon used to construct amino acids came from terrestrial foods.     

Dogs,divers, deer and diet. Stable isotope results from Star Carr and a response to Dark

We here respond to Dark's [Dark, P., 2003. Dogs, a crane (not duck) and diet at Star Carr: a response to Schulting and Richards. Journal of Archaeological Science 30, 1353–1356] criticisms of our previous paper [Schulting, R.J., Richards, M.P., 2002. Dogs, ducks, deer and diet: a reappraisal of the stable isotope evidence on early domestic dogs from the Vale of Pickering, north-east England. Journal of Archaeological Science 29, 327–333] in which we supported the previous interpretation of stable isotope data from the Seamer Carr dog [Clutton-Brock, J., Noe-Nygaard, N., 1990. New osteological and C-isotope evidence on Mesolithic dogs: companions to hunters and fishers at Star Carr, Seamer Carr and Kongemose. Journal of Archaeological Science 17, 643–653] as indicating a marine-influenced diet. Additional isotopic data are presented on fauna from Star Carr, most importantly from two piscivorous diving birds. These new data further support the original contention that marine protein is the most likely source of the elevated carbon and nitrogen isotope values seen in the Seamer Carr dog. In addition, cutmarks on the humerus of a Brent goose, and the presence of an additional element (right femur) attributable to the previously known adult dog from Star Carr, are noted for the first time. Two new AMS determinations are presented for Star Carr, placing an adult dog in the period 9680 ± 55 BP (9270–8840 cal BC) and a subadult dog somewhat later at 9342±41 BP (8735–8475 cal BC). Unlike Seamer Carr, both dogs show entirely terrestrial (non-marine) diets.     

Stable isotope analysis of Pacific rat (Rattus exulans) from archaeological sites in Mangareva (French Polynesia): The use of commensal species for understanding human activity and ecosystem change

Stable carbon and nitrogen isotope analysis was applied to archaeological specimens of the commensal Pacific rat (Rattus exulans) to investigate nutrient fluxes in prehistoric socio-ecosystems on Mangareva (Gambier Islands) and their implications for anthropogenic environmental change. The Pacific rat – ubiquitous in Polynesian archaeological sites – is characterised by low dietary selectivity and a limited home range, making it an ideal candidate for assessing changes in island food webs. Temporal trends in diet-derived bone collagen δ¹³C and δ¹⁵N values are assessed from three sites: The Onemea Site, Taravai Island (TAR-6), Nenega-iti Rockshelter, Agakauitai Island (AGA-3) and Kitchen Cave Rockshelter, Kamaka Island (KAM-1). An overall trend of decreasing δ¹⁵N values in rat bone collagen over time reflects archipelago-wide changes to island socio-ecosystems most likely resulting from seabird population declines. Differences in site function and human activity may have also influenced local rat dietary patterns. Stable isotope analysis of the Pacific rat provides a low-impact line of evidence towards the reconstruction of human-centred food webs and the flow of nutrients within island socio-ecosystems.     

Nitrogen isotopes and the trophic level of humans in archaeology

Human and domesticate animal bone collagen δ¹⁵N values in prehistory differ generally by 3‰ or more from Neolithic to post- Roman times in Northwest Europe, leading to an assumed dietary animal protein fraction of 60–80% using a standard interpretation of δ¹⁵N values. We examine the assumptions on which this model rests and the limitations of our knowledge in the analysis of δ¹⁵N values in archaeology. We have developed a set of models which, with small changes made in assumptions (on the order of 1‰), can produce substantially lower estimates of the dietary animal protein fraction for given δ¹⁵N values. We consider the implications of various dietary animal protein fractions on agricultural carrying capacities and human population densities in prehistory.     

Human and animal diet at Conchopata, Peru: stable isotope evidence for maize agriculture and animal management practices during the Middle Horizon

This paper reports δ¹³C and δ¹⁵N values for human and animal skeletal remains from the Middle Horizon (AD 550–1000) site of Conchopata in the Peruvian highlands. The data indicate that maize was the dietary staple for both humans and the majority of animals at this urban site. Camelids at the site segregated into two groups according to δ¹³C values, reflecting two distinct animal management strategies. Human infants were found to have elevated δ¹⁵N values, reflecting the trophic level effect of breastfeeding. No sex based differences in diet were observed.     

Stable isotope analysis of human and animal remains from the Late Upper Palaeolithic site of Balma Guilanyà, southeastern Pre-Pyrenees,Spain

Stable isotope analysis of carbon (¹³C/¹²C) and nitrogen (¹⁵N/¹⁴N) was performed on collagen extracted from three human and five herbivore bone and tooth samples from the Late Upper Palaeolithic site of Balma Guilanyà (Catalonian Pre-Pyrenees, Spain). Contextual and palaeoecological data as well as radiocarbon dates indicate that the studied occupation phase took placed during the Bolling/Allerod interstadial (GI-1a event). The human remains were co-mingled without any anatomical association, corresponding to a minimum number of three individuals, and it was not possible to determine if the three analyzed samples are from one or more individuals. The mean isotope values obtained from the human remains are δ¹³C = −19.8‰ and δ¹⁵N = 6.7‰, while those of the large herbivores (red deer and wild goat) were −19.8‰ and 1.7‰ for δ¹³C and δ¹⁵N respectively. This indicates that the main source of protein in the diet of the Balma Guilanyà human(s) came from terrestrial herbivores. There is no zooarchaeological or isotopic evidence for the consumption of freshwater or marine resources at the site, which lies 80 km from the present Mediterranean coast. The low δ¹⁵N values observed in both human and animal samples correspond to a trend reported by other researchers working in northwestern Europe: a significant δ¹⁵N reduction in collagen from bones datable within 20,000–10,000 BP, followed by a rise to present values in the Early Holocene. This phenomenon, generally attributed to climatic and/or pedological processes, had not been previously observed in the Mediterranean region and, until now, was thought to be restricted to northern Europe.     

Stable isotope chemistry,population histories and Late Prehistoric subsistence change in the Aleutian Islands

Aleut population history has been a topic of debate since the earliest archaeological investigations in the region. In this paper, we use stable isotope chemistry to evaluate the hypothesis that two distinct groups of people, Paleo- and Neo-Aleut, occupied the eastern Aleutians after 1000 BP. This study focuses on 80 sets of directly dated eastern Aleutian burial assemblages from Chaluka midden, Shiprock Island and Kagamil Island. We use a linear mixing model informed by isotopic analysis of two large Aleut faunal assemblages to address temporal and spatial variation in human carbon and nitrogen stable isotope data from these sites. The patterning we report addresses both Aleut demographic and economic prehistory, illustrating a transition in both at ca. 1000 BP. Our results suggests that the Chaluka diet, dominated by Paleo-Aleut inhumations, differed in both trophic level and foraging location from the other two sites for much of the past 4000 years. Trends in our data also suggest that individuals from Shiprock and Kagamil burial caves, primarily Neo-Aleuts, had enough access to higher trophic level foods to differentiate their bone chemistries from those buried in Chaluka midden. These trends in diet, recently reported genetic differences, as well as the introduction of novel mortuary practices at ca. 1000 BP, suggest that Neo-Aleuts do represent a population new to the eastern Aleutians.     

Mummies, maize, and manure: multi-tissue stable isotope analysis of late prehistoric human remains from the Ayacucho Valley, Perú

This paper reports the δ¹³C and δ¹⁵N values of bone collagen, muscle and skin from several late prehistoric–early colonial (AD 1490–1640) mummies from Perú's Ayacucho Valley. The mean of the δ¹³C and δ¹⁵N values of bone collagen are −11.5 ± 1.4 and 11.1 ± 0.7‰, respectively. The mean of the δ¹³C values for Vinchos skin is −11.8 ± 1.2‰ and the mean of δ¹⁵N values is 13.2 ± 0.5‰. The samples of muscle tissue have a δ¹³C mean of −11.9 ± 0.9‰ and a δ¹⁵N mean of 12.7 ± 0.3‰. The data from bone collagen indicate maize was the basis of the region's subsistence economy. A significant correlation between δ¹³C and δ¹⁵N values of bone collagen (R² = 0.75) is consistent with the preferential fertilization of maize with composted manure. Both skin and muscle samples are consistently enriched in δ¹⁵N relative to paired samples of bone (2.1 ± 0.5 and 1.6 ± 0.7‰, respectively), possibly as a result of short term physiological stress or differential decomposition.     

Stable carbon and nitrogen isotope analysis on human remains from the Early Mesolithic site of La Vergne (Charente-Maritime,France)

We report here the results of stable carbon and nitrogen isotope analysis of human and faunal remains from La Vergne (Charente-Maritime, western France), a rare Early Mesolithic burial site (ca. 8500–8000 cal BC). The results for nine humans (average δ¹³C = −19.3‰; δ¹⁵N = 9.4‰) indicate a strongly terrestrial diet, dominated by animal protein, with the possibility of, at best, a slight contribution of marine-derived protein. Given lower sea-levels in the early Holocene, the site would have been some 60–80 km from the sea at the time of its use; nevertheless, contacts with the coast are shown by the presence of numerous marine shell beads in the graves. In the light of the stable isotope results, it is suggested here that such contacts most likely took the form of exchange with coastal communities whose remains now lie underwater.     

Oxygen Isotopes and Human Residential Mobility in Central Western Argentina

Stable oxygen isotopes (δ¹⁸O) from human bone apatite from central western Argentina (30º–37ºS latitude) were analysed to understand changes in human residential mobility during the Late Holocene. This region contains evidence for the use of domesticated plants over the last 2000 years (Zea mays, Cucurbita sp. and Phaseolus sp., among others), and previous models of prehistoric occupation have suggested a distinct change in mobility and population movement associated with their incorporation. The importance of these domesticates also seems to have varied geographically, being greater in the northern part of the region and declining as one moves south toward the limit with Patagonia. Expectations about patterns of residential mobility have varied accordingly. To better evaluate these models, we analysed carbonate δ¹⁸O from the bone apatite of 71 individuals with radiocarbon dates spanning the last 6000 years. Given the existing evidence, we expected to see temporal and geographic differences in their oxygen isotope values associated with changes in residential mobility and the incorporation of different sources of drinking water available within the region. These expectations were not met. Significant variations were seen across all samples compared, both temporal and geographic, with no discernible differences among them. The data suggest that populations throughout the area were all highly mobile, and that this did not change with the incorporation of domesticates. Copyright © 2011 John Wiley & Sons, Ltd.     

Stable carbon and nitrogen isotope variations in tooth dentine serial sections from Wharram Percy

Here we report δ¹³C and δ¹⁵N measurements of serial sections of human deciduous and permanent tooth dentine from archaeological samples taken from the medieval village site of Wharram Percy, Yorkshire, UK. We found a pattern of enrichment, for both δ¹³C and δ¹⁵N, where the tooth crown was greater than the cervical part of the root, which in turn was greater than the apical portion of the root and the associated rib collagen values. This pattern reflects a decrease in the consumption of isotopically enriched breast milk and the introduction of less enriched weaning foods in the diet. The (mean±SD) difference between the deciduous second molar crowns and corresponding rib samples from the same individuals after 2 years of age was 1.2±0.4‰ for δ¹³C and 3.2±0.8‰ for δ¹⁵N. The δ¹⁵N values are as predicted, but as there were no C₄plants at Wharram Percy, this 1.2‰ enrichment in δ¹³C represents clear evidence of a carbon trophic level effect in collagen from breastfeeding infants. Carbon and nitrogen results also show that the infant diet among those who died in infancy did not differ from those who survived into childhood. This study demonstrates the promise of using dentine serial sections to study the temporal relationships of breastfeeding, weaning, and dietary patterns of single individuals.     

Fossil dogs and wolves from Palaeolithic sites in Belgium,the Ukraine and Russia: osteometry,ancient DNA and stable isotopes

Using multivariate techniques, several skulls of fossil large canids from sites in Belgium, Ukraine and Russia were examined to look for possible evidence of the presence of Palaeolithic dogs. Reference groups constituted of prehistoric dogs, and recent wolves and dogs. The fossil large canid from Goyet (Belgium), dated at c. 31,700 BP is clearly different from the recent wolves, resembling most closely the prehistoric dogs. Thus it is identified as a Palaeolithic dog, suggesting that dog domestication had already started during the Aurignacian. The Epigravettian Mezin 5490 (Ukraine) and Mezhirich (Ukraine) skulls are also identified as being Palaeolithic dogs. Selected Belgian specimens were analyzed for mtDNA and stable isotopes. All fossil samples yielded unique DNA sequences, indicating that the ancient Belgian large canids carried a substantial amount of genetic diversity. Furthermore, there is little evidence for phylogeographic structure in the Pleistocene large canids, as they do not form a homogenous genetic group. Although considerable variation occurs in the fossil canid isotope signatures between sites, the Belgian fossil large canids preyed in general on horse and large bovids.     

The fish of Lake Titicaca: implications for archaeology and changing ecology through stable isotope analysis

Research on past human diets in the southern Lake Titicaca Basin has directed us to investigate the carbon and nitrogen stable isotopes of an important dietary element, fish. By completing a range of analyses on modern and archaeological fish remains, we contribute to two related issues regarding the application of stable isotope analysis of archaeological fish remains and in turn their place within human diet. The first issue is the potential carbon and nitrogen isotope values of prehistoric fish (and how these would impact human dietary isotopic data), and the second is the observed changes in the fish isotopes through time. Out of this work we provide quantitative isotope relationships between fish tissues with and without lipid extraction, and a qualitative analysis of the isotopic relationships between fish tissues, allowing archaeologists to understand these relationships and how these values can be applied in future research. We test a mathematical lipid normalization equation to examine whether future researchers will need to perform lipid extraction procedures for Lake Titicaca fish. We also analyze a number of aquatic plants to better understand the range of isotopic signatures of the Lake Titicaca ecosystem. We use these data to better understand prehistoric human diet and the role that fish may have played in the past as well as potential changes in local lake ecology through time.     

The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices

Recent archaeological studies of human diet have used stable nitrogen isotope ratios (δ¹⁵N) from human bone collagen to infer the relative importance of terrestrial plant and animal foods. This approach is based on widely observed enrichment of δ¹⁵N up the food chain, plants having distinctly lower values than the herbivores that consume them. Studies of early farming diets in Britain, Denmark and Germany have tended to detect relatively high δ¹⁵N values (e.g. c. +9‰), interpreted as evidence of a diet largely based on animal products, though archaeobotanical evidence for crop cultivation (e.g. carbonised cereal grain and chaff) is widespread. This paper investigates the impact of manuring on δ¹⁵N values in modern cereals, and of charring on these cereal values. The results from two long-term experiments demonstrate that manuring significantly raises δ¹⁵N in cereal grain and chaff. Depending on manuring levels and frequency, it appears that human diets with a major component of such grain would conventionally be interpreted as indicating a largely animal-based diet or a mixed plant/animal diet. Moreover, preliminary analyses of experimentally charred grain and chaff from manured and unmanured conditions are promising for the extraction of reliable ancient δ¹⁵N values from archaeobotanical cereal remains. The wider implications of these results, and the need for further work, are discussed.