Using ancient DNA identification and osteometric measures of archaeological Pacific salmon vertebrae for reconstructing salmon fisheries and site seasonality at Dionisio Point,British Columbia

Determining the diversity of salmon species represented in archaeological contexts is critical to understanding patterns of salmon harvesting and consumption, storage practices and seasonal patterns of resource exploitation by precontact peoples of the Northwest Coast. We present species determinations generated for salmon vertebrae from the Dionisio Point site (DgRv-003) in coastal southwestern British Columbia, a well-preserved village site that dates to between 1500 and 1300 cal BP. Molecular species identification was possible for 70 Pacific salmonid vertebral elements recovered from one excavated plankhouse at the site. Coupled with osteometric measures, we determine that residents of the plankhouse fished primarily for chum, sockeye and pink salmon, with a lesser emphasis on chinook, coho and steelhead. These results indicate a diverse fishery that likely occurred between July and December, suggesting a mid summer through winter season of occupation for the village.     

Can salmonids (Oncorhynchus spp.) be identified to species using vertebral morphometrics?

Remains of anadromous Pacific salmon and trout (genus Oncorhynchus) are common in archaeological sites from California to Alaska; however, morphological similarity generally precludes species identification, limiting the range of questions that salmonid remains can address in relation to past human use and ongoing efforts in conservation biology. We developed a relatively simple, rapid, and non-destructive way to classify salmon and trout vertebrae from archaeological contexts to species using length, height and the ratio of length to height. Modern reference material was obtained from all seven anadromous Oncorhynchus species native to the west coast of North America. A minimum of ten adult Chinook (Oncorhynchus tshawytscha), chum (Oncorhynchus keta), coho (Oncorhynchus kisutch), pink (Oncorhynchus gorbuscha), and sockeye salmon (Oncorhynchus nerka) and cutthroat (Oncorhynchus clarki clarki) and steelhead trout (Oncorhynchus mykiss) were skeletonized and vertebra length and height were measured. Morphometric analyses compared species classification success based on Linear Discriminant Analysis (LDA), Classification and Regression Trees (CART), and randomForest, with CART performing the best. Classification analyses used all seven species individually, but because of considerable overlap among several species we also conducted analyses on four species groupings. We assigned Chinook salmon and cutthroat to their own groups based on their dissimilarities from each other and the other species. The remaining species were divided into two group complexes (a) chum, coho, and steelhead; and (b) pink and sockeye. When we grouped species according to similar morphology, CART overall success rates increased, ranging from 92 to 100%. Individual species with the highest successful classification rates using CART were Chinook salmon and cutthroat, from 92 to 100%, respectively. We applied our classification to an assemblage of ancient (1000–3000 year old) salmonid vertebrae from the Swiftwater Rockshelters excavations on the upper Wenatchee River in Washington State, U.S.A.     

Ancient DNA investigation of prehistoric salmon resource utilization at Keatley Creek,British Columbia,Canada

This study applied ancient DNA techniques to achieve accurate species identifications for the archaeological salmon remains recovered from the prehistoric pithouse village of Keatley Creek in British Columbia, Canada. Previous archaeological studies indicate that economic stratification within the community might have resulted in differential access to some preferred salmon species, such as sockeye and chinook. Unambiguous ancient DNA species identification now makes it possible to more accurately address the issue of early salmon resource utilization in the region. This study analyzed 60 salmon remains from two specialized structures and two residential structures in order to identify any species differences among bony salmon remains found within the structure. Although high success rates (over 90%) were obtained for ancient DNA tests, only three species (chinook, sockeye and coho salmon) were identified from the remains. Pink salmon was not identified among the tested sample, despite the fact that it was originally assumed to be a staple species for the site's native inhabitants. The absence of pink salmon in our sample significantly altered the picture of early salmon fishing activities in the region. As a result, the effects of economic stratification on differential access to the remaining so-called preferred species of sockeye and chinook within the four structures studied were not as dramatic as previously thought, although differences among the structures could still be observed.     

Continuity Amidst Change: Village Organization and Fishing Subsistence at the Dionisio Point Locality in Coastal Southern British Columbia

Shifts over time in faunal representation in Northwest Coast archaeological sites have been viewed as reflecting changes in economic organization and social complexity. We use fish faunal data from the Parry Lagoon Midden (site DgRv-006) in the Salish Sea region of coastal southwestern British Columbia, Canada, to evaluate the relationship between change in subsistence strategies and community organization. We describe and compare fish assemblages recovered from a shell midden generated by habitation at two sequentially occupied village sites. The lower midden layers include faunal remains derived from the Marpole Period plankhouse village occupation at site DgRv-003 (1500 to 1300 cal BP), while the upper layers were generated during the occupation of a Late Period plankhouse within site DgRv-006 (1000 to 600 cal BP). We compare the two faunal records to ascertain if subsistence re-organization accompanied the social change associated with abandonment and later re-establishment of a village in the Dionisio Point Locality. Results indicate continuity of a diverse fishing economy for the two occupations, and therefore across the Marpole to Late Period transition. This study adds to the recent trend in Northwest Coast archaeology to reconsider the long-standing view that social and subsistence change necessarily occurred in tandem.     

Chinook Salmon,Late Holocene Climate Change,and the Occupational History of Kettle Falls,a Columbia River Fishing Station

ABSTRACT

Kettle Falls, located 1125?km from the mouth of the Columbia River in present-day Washington State (USA), was the second-most important salmon fishing and trading locus on that river in the early historic era. We encapsulate the late Holocene history of the fishery by deriving a summed probability distribution function (SPDF) from 50 radiocarbon ages from 13 archaeological sites within 2?km of the falls. When compared to an SPDF from 307 sites from elsewhere on the Columbia Plateau, and a null model, the Kettle Falls SPDF exhibits two phases of elevated activity at 1700–1300?cal BP and 800–500?cal BP, and an intervening lull. These phases are not related to the excavation history or differential exposure of sites to taphonomic processes, but they are concordant with episodes of glacial advance in the local mountains, which reflect hemispheric-scale changes in climate. Modern returns of summer-run Chinook salmon to the Columbia River are inversely correlated with sea-surface temperature regimes in the northeast Pacific Ocean, and we propose that the occupational history of the Kettle Falls fishery echoes long-term variations in the returns of salmon to the upper Columbia River linked to climate change.     

Magnetic Gradient Survey of the Marpole Period Dionisio Point (DgRv-003) Plankhouse Village,Northwest Coast of North America

Architectural reconstructions of plankhouses are central to the study of household and community organization on the Northwest Coast of North America. However, the sample of substantially excavated houses is constrained by their size, stratigraphic complexity, and typically limited surface expression. We present the results of a magnetic gradiometry survey of the Dionisio Point site (DgRv-003) village, occupied ca. a.d. 500–700 on the coast of British Columbia, Canada. Survey of four house platforms reveals patterning of magnetic anomalies consistent with the structure of shed-roof houses, a design recorded ethnographically and identified archaeologically at the site. These results suggest a consistent pattern of spatial and, potentially, social organization of the households. The similarity of patterns suggests that magnetometry may be useful for guiding plankhouse excavations elsewhere on the Northwest Coast, providing a means for expanding our knowledge of houses without relying solely on traditional excavation methods.     

Faunal remains from radiocarbon-dated soils within landslip debris from The Undercliff,Isle of Wight,Southern England

An examination of coastal sections of landslip material forming The Undercliff of the Isle of Wight has revealed the presence of buried soils, in places, within the debris. Critical sections at Binnel Point and St. Catherine's Point have been logged and the contained vertebrate and molluscan faunas analysed. The Mollusca from both sites are very similar and indicate a shaded environment, contrasting sharply with the immediate local environments today. The vertebrates, present only at Binnel Point, again suggest woodland. The presence of red squirrel (Sciurus vulgaris) and dormouse (Muscardinus avellanarius) is especially noteworthy constituting their earliest and only secure British fossil records respectively. Some marine shells (Patella, Gibbula) and remains of larger vertebrates (including red deer) possibly represent midden debris. Charcoal from the lower soil at Binnel Point yielded a radiocarbon date of 4480 ± 100 bp (BM-1737) indicating that at least some of the middens at Binnel date from the Neolithic, much earlier than previously demonstrated. The sections at St. Catherine's Point provided no evidence of human occupation but yielded two further radiocarbon dates of 4490 ± 40 bp (SRR-1813) and 3960 ± 50 bp (SRR-1947) from wood incorporated within the landslips. The significance of these dates in relation to the history of landslipping is discussed.     

“After 20 Years”: A Taphonomic Re-evaluation of Nahal Hadera V,an Epipalaeolithic Site on the Israeli Coastal Plain

We carried out a detailed taphonomic study of the faunal remains from Nahal Hadera V (NHV), a large Kebaran open-air site on the northern coastal plain, Israel. Faunal remains were previously identified to bone element and species (Saxon, Martin & Bar-Yosef, 1978). However, NISP and MNI values were calculated based on teeth and epiphyses only. In this study we identified the maximum number of skeletal elements, including head fragments, vertebrae, ribs, carpals/tarsals, and shaft fragments. Further analyses included search for various bone surface modifications and the study of mode of bone fragmentation. We found minor loss of bones caused by various diagenetic processes, and no signs of carnivore activity. These results suggest that most of the bone destruction was caused during time of occupation, probably for marrow consumption, as indicated by mode of bone fragmentation. Absence of selective transport for gazelle (Gazella gazella) and fallow deer (Dama mesopotamica), the major prey species (with 70% and 20%, respectively), is supported by homogeneous representation of head-limb-toe elements. However, the carcasses may have been processed before transport to the site. Thus, a taphonomic analysis from NHV helps to illuminate the economy of NHV inhabitants and fine-tunes our understanding of the depositional history of the site. However, it does not overthrow results of earlier research using classical methods.     

Palaeoenvironmental reconstruction of La Olla,a Holocene archaeological site in the Pampean coast (Argentina)

The evolution of littoral palaeoenvironments in the southern Pampas region of Argentina towards the end of the early Holocene and the beginning of the mid Holocene is discussed, and the formation processes of the archaeological site of La Olla are analysed. This site is located in the intertidal zone of the present beach and is remarkably well-preserved. The human occupation at the site has been dated at between 7400 and 6480 yrs BP, and is interpreted as a place for the processing and consumption of the southern fur seal (Arctocephalus australis) and southern sea-lion (Otaria flavescens). It is concluded that biostabilization by microbial mats of the sedimentary structures and archaeological remains that developed and are present on the tidal flat prevented their erosion by wind, turbulence currents and waves. This is what has permitted the exceptional preservation of the site. On the basis of the analyses of one of the sectors of the site it can be concluded that the sedimentary succession of the La Olla site is the result of the coastal environmental changes during the early–mid Holocene in the pre-maximum transgressive episode. During this interval a tidal flat developed on the Pleistocene abrasion platform. Human occupation occurred during the formation of mixohaline marsh, an upper intertidal–lower supratidal zone ecosystem.     

Global human impacts or climate change?: explaining the Sooty Shearwater decline at the Minard site,Washington State,USA

Sooty Shearwater (Puffinus griseus) remains decrease throughout the occupation (AD 1000–1600) of the Minard site (45-GH-15) on the southern Washington coast, USA. Given that New Zealand is one of the major breeding locations for this long-distant migrant, the decline at the Minard site may be due to human impacts on a global scale. In particular, the Maori practice of muttonbirding, the killing and preparation of fledgling shearwaters for storage, may have affected the number of summer migrants reaching the Pacific Northwest Coast. A second hypothesis attributes the decline in Sooty Shearwaters at the site to increasing sea surface temperatures and/or increased frequency of ENSO events during this period. Understanding how Sooty Shearwaters responded to human hunting and/or climate events in the past may be useful for the current management of this species, which has faced dramatic declines in recent decades.     

Stratigraphy and geochronology of La Playa archaeological site,Sonora, Mexico

La Playa archaeological site in northern Sonora, Mexico contains a long record of human activity that includes the Paleoindian period (terminal Pleistocene) and much of the Holocene. The size and complexity of La Playa has discouraged a systematic characterization of its stratigraphy and geochronology, a deficiency we redress in the study. We distinguished seven stratigraphic units ranging in age from >44,570 to 400 cal yr B.P. using ¹⁴C dates from charcoal and terrestrial gastropods found mostly in archaeological features. All of the buried (in situ) cultural remains are contained in Units B (4690–1580 cal yr B.P.) and C (1010–400 cal yr B.P.) and represent overbank deposition from the nearby Rio Boquillas. Occupation at the site peaks in Units B₄ and B₅, corresponding to the Cienega phase (2800–1800 cal yr B.P.) of the Early Agricultural period. This period coincides with the growth of early agricultural villages in the region and is marked at La Playa by thousands of archaeological features including roasting pits, human burials, and extensive canal irrigation systems. The presence of semi-aquatic and aquatic snails demonstrates that water was present year round in the canal systems constructed during this period. Stable and radiometric isotopic evidence suggests that early agriculturalists diverted ground water over several kilometers from the nearby Rio Boquillas. The extensive Cienega phase occupation ended after about 1700 cal yr B.P. with deep erosion of the site, an event also visible in alluvial records in southern Arizona that marked the end of the Early Agricultural period and significant changes in settlement organization in the region.     

Assessing Protocols for Identifying Pacific Island Archaeological Fish Remains: The Contribution of Vertebrae

Three fish bone identification protocols used for determining taxa composition for Pacific island archaeofaunal assemblages are evaluated. The protocols include using the following: (1) the most commonly identified five paired cranial bones and ‘specials’ or unique elements; (2) an expanded number of cranial bones; and (3) the less common inclusion of all vertebrae. Explicit identification and quantification protocols are outlined for systematically incorporating all vertebrae which, predictably, increases the number of identified specimens for an assemblage, thus providing more bones useful for reconstructing live fish biomass (weight and length). Significantly, a range of unique archaeological vertebrae are useful for calculating minimum number of individuals. Using a well‐preserved assemblage from Henderson Island, Pitcairn Group, southeast Polynesia, numbering 6480 fish bones (concentration index = 21 580 m³), we demonstrate differences in rank‐order abundance from three taxon identification protocols. For example, when using all vertebrae grouper (Serranidae) and surgeonfishes (Acanthuridae) are more numerically equivalent than when relying mostly on cranial bones for identification for minimum number of individuals and number of identified specimens. This has important implications for making comparisons between sites or across regions where different identification protocols were used. This pilot study demonstrates that using all vertebrae for taxon identification and quantification, not just unique hypurals (terminal vertebrae) or those from sharks and rays (Elasmobranchii), should be standard practice for identifying a greater number of bones to taxon and thereby providing better reconstructions of prehistoric fishing and subsistence practices in the Pacific. Copyright © 2013 John Wiley & Sons, Ltd.     

Environment and Subsistence of the Early Inhabitants of Coastal Southwestern Nigeria

From where did the early inhabitants of the Badagry coastal area of southwestern Nigeria originate? Has the area been occupied from “ancient times,” as claimed by oral traditions? What was the nature of the environment and subsistence of these early inhabitants? Excavations at Apa, west of Badagry, provided answers to these questions. A radiocarbon date of 2670 ± 90 bp showed that human occupation there is at least 3000 years old, and implied occupation during the Late Stone Age. The occupation was in two phases: a prehistoric phase, during which Apa site 1 (Ap1) was occupied, and an historic phase, with occupation of Apa site 2 (Ap2). Sedimentological and archaeological data also showed that the environment at the beginning of occupation was similar to today. The presence of a groundstone axe, charred palm kernels, and charcoal at the Ap1 site is interpreted to imply the clearing and burning of vegetation, probably preparatory to planting and the exploitation of oil palm (Elaeis guineensis) from about 2670 ± 90 bp. D'où sont venus les premiers habitants de la région côtiere Badagry du sud-ouest du Nigeria? Est-il vrai que la région a été occupé depuis les temps anciennes, comme confirme les traditions orales? Quelle étaient la nature de l'environnement et du subsistence de ces habitants préhistoriques? Des fouilles archéologiques à Apa, a l'ouest de Badagry, ont fourni des réponses nécessaires à ces questions. Un date radiocarbone de 2670 ± 90 bp a montré que les activités humaines de ce site date au moins de 3000 années, qui indique que l'occupation des habitants à c'endroit étais durant la periode de l'Age de la Pierre Recent. L'occupation de cette region était en deux phases: un phase prehistorique, la période pendant lequelle le site d'Apa 1 (Ap1) était occupé, et la phase historique, qui a marqué l'occupation du deuxième site d'Apa 2 (Ap2). Les données de la sedimentologie et de l'archéologie ont aussi montrent que l'environnement au commencement d'occupation était similaire de nos jours. On pense que la presence d'un hache “groundstone,” de l'amande de palmier et du charbon à la site d'Ap1 indiques la déstruction de la vegetation, preparant probablement la terre pour la semence et l'exploitation du l'huile de palme (Elaeis guineensis) d'environ de 2670 ± 90 bp.     

Pre-Columbian Fishing on the Coast of the Atacama Desert,Northern Chile: An Investigation of Fish Size and Species Distribution Using Otoliths From Camarones Punta Norte and Caleta Vitor

The bountiful marine resources of the northern Chilean coast offset the extreme aridity of the Atacama Desert in pre-Columbian times, underwriting permanent human occupation, and providing the basis for a long tradition of marine subsistence. We analyzed fish otoliths (n = 549) recovered from the sites of Camarones Punta Norte (occupied ca. 7,000–5,000 years ago) and Caleta Vitor (occupied ca. 9,500–300 years ago) to investigate species distribution and changes over time. We also estimated the size of the fish based on relationships between otolith weight and fish total length (TL) obtained from modern samples of the predominant species, Sciaena deliciosa. The estimated size range of S. deliciosa from Caleta Vitor included fish that were significantly larger than those from Camarones Punta Norte, with the maximum TL (970 mm) almost double the modern maximum length documented. The fluctuating abundance of fish species and other marine taxa from Camarones Punta Norte indicates intense but sporadic use of the site over the span of occupation. In contrast, human occupation of the Caleta Vitor estuary is more continuous. Comparisons of the fish assemblages with a nearby contemporaneous site, Quebrada de los Burros in southern Peru, suggest that fishing technologies were similar along this section of the Pacific coast.     

Determining the habitats of mollusk collection at the Sapelo Island shell ring complex, Georgia, USA using oxygen isotope sclerochronology

Sequential oxygen isotope analysis was performed on Mercenaria spp. (n = 41) and Crassostrea virginica (n = 17) valves excavated from the Sapelo Island Shell Ring complex, coastal Georgia, USA. The data were analyzed to determine the habitats in which the mollusks were harvested in order to gain insight into the subsistence practices of the site occupants. The rationale for this study is based on the observation that temperatures are similar in all of the habitats surrounding the island, but the δ¹⁸O_water trends across the habitats co-vary with salinity. Accordingly, the shells grown in these habitats contain sinusoidal oscillations in δ¹⁸O through ontogeny due primarily to seasonal temperature variation, but the absolute values reflect the salinity ranges of each habitat. Assuming a relatively constant oxygen isotope/salinity gradient since the time of site occupation, it appears that both of these mollusks were exploited over nearly the full range of their salinity tolerances. While a precise estimate of travel distance during collection cannot be made, these data suggest that mollusks were harvested at distances from the site greater than would be predicted using terrestrial foraging models. In addition to providing insight into past subsistence strategies in this region, this research establishes a new method of assessing catchment area and mobility practices.     

Tracking Fish and Human Response to Earthquakes on the Northwest Coast of Washington State,USA: A Preliminary Study at Tse-whit-zen

Evidence of large earthquakes occurring along the Pacific Northwest Coast is reflected in coastal stratigraphy from Oregon to British Columbia, where there also exists an extensive archaeological record of Native American occupation. Tse-whit-zen, a large Native American village dating between ～2800 yrs BP and the historic era, located on the Olympic Peninsula of Washington State, was excavated with exceptionally fine stratigraphic control allowing for precise comparison of these natural and cultural records. Here we report on the ～10,000 fish remains from one 2 × 2 m excavation block; this assemblage spans the timing of one seismic event, allowing study of changes in relative taxonomic abundance through time that may coincide with earthquakes or other environmental changes. Results indicate a wide variety of fish taxa were used throughout the dated occupation. Comparisons of fish use before and after one earthquake event shows a decline in salmon (Oncorhynchus sp.) and an increase in herring (Clupea pallasii), shifts consistent with earthquake-related habitat loss. This serves as a pilot study for a large-scale collaborative project that is drawing on the range in animal types (invertebrates, mammals, birds, and fishes) to assess human response to gradual and abrupt environmental change at Tse-whit-zen.     

An ongoing Austronesian expansion in Island Southeast Asia

The Austronesian expansion into Island Southeast Asia and the Pacific was the last and most far-reaching prehistoric human migration. Austronesian languages replaced indigenous languages over nearly half the globe, yet the absolute number of Austronesian colonists was small. Recently, geneticists have identified large geographic disparities in the relative proportions of Asian ancestry across different genetic systems (NRY, mitochondrial DNA, autosomes and X chromosomes) in Austronesian-speaking societies of Island Southeast Asia and the Pacific. Surprisingly, a substantial genetic discontinuity occurs in the middle of a continuous chain of islands that form the southern arc of the Indonesian archipelago, near the geographic center of the Austronesian world. In the absence of geographic barriers to migration, this genetic boundary and swathe of Austronesian language replacement must have emerged from social behavior. Drawing on decades of comparative ethnological research inspired by F.A.E. van Wouden’s structural model of Austronesian social organization, later codified by Claude Lévi-Strauss as “House societies” (“sociétés à maison”), we propose a two-stage ethnographic model in which the appearance of matrilocal “House societies” during the initial phase of the Austronesian expansion, and the subsequent disappearance of “House societies” in lowland rice-growing regions, accounts for the observed linguistic, genetic and cultural patterns.     

The Varying Role of Vertebrae in Pacific Fishbone Analysis: Comparing Tropical Versus Temperate Midden Assemblages

Archaeologists working in the tropical Pacific have demonstrated the feasibility and value of including fish vertebrae in midden analyses, and recent New Zealand studies draw similar conclusions. This work provides an illustrated guide to the identification of vertebrae from key New Zealand fish taxa and shows the effects of including vertebrae on a large fishbone assemblage from southern New Zealand. We note major differences between New Zealand and tropical Pacific assemblages resulting from the inclusion of vertebrae. Unlike the Indo‐Pacific taxa of the tropical Pacific, no New Zealand species have been shown to be sensitive to the inclusion of vertebrae. In both places, including vertebrae results in changes in relative abundance and rank order; but in New Zealand, this is a function of processing practices, not fishing behaviours. This work serves to highlight changes in the Polynesian fishing adaption following the colonisation of New Zealand. Copyright © 2017 John Wiley & Sons, Ltd.     

Independent confirmation of a diagnostic sheep/goat peptide sequence through DNA analysis and further exploration of its taxonomic utility within the Bovidae

Buckley et al. (2010) recently identified a Type I collagen (COL1A2) peptide sequence that discriminates between sheep and goat species. The exact location of this peptide sequence on the genome was not reported. We identified the sequence's location and developed a PCR based approach that amplifies the diagnostic sequence from exon 41 of the COL1A2 gene. Using DNA analysis, we confirmed that this COL1A2 peptide discriminates between goat and sheep species reliably, but is limited as a more general phylogenetic marker.     

Preliminary U-series and Thermoluminescence dating of excavated deposits in Liang Bua sub-chamber,Flores, Indonesia

We report the results of a test excavation of deposits in a limestone cave sub-chamber located beneath the main chamber of Liang Bua, Flores, Indonesia; the discovery site of the small hominin species, Homo floresiensis. Well-preserved remains of extinct Pleistocene fauna and stone artefacts have previously been identified on the surface of a sediment cone within the sub-chamber. Our excavation of the deposits, at the base of the sediment cone in the sub-chamber (to 130 cm depth) yielded only a few fragmentary bones of extant fauna. Uranium/Thorium (U-series or U/Th) dating of soda straw stalactites excavated from 20 to 130 cm in depth demonstrates that the excavated sediments were deposited during the Holocene. Red Thermoluminescence (TL) dating of the sediments at the base of the excavation (130 cm depth) indicates these sediments were last exposed to sunlight at 84 ± 15 ka (thousand years), similar to red TL ages of cave sediments from the main chamber. Together, these results indicate that the surface faunal remains, which are morphologically analogous to Pleistocene finds from the main chamber excavations, were transported to the sub-chamber relatively recently from the main chamber of Liang Bua and probably originated from conglomerate deposits at the rear of the cave and from deposits around the front entrance. There is no evidence for hominin occupation of the sub-chamber, instead it seems to have acted as a sink for cultural materials and fossil remains transported from the surface via sinkholes. Despite the small number of finds from the test excavation, it is possible that more extensive excavations may yield additional transported cultural and faunal evidence at greater depths.