Methods for the Study of Ancient Hair: Radiocarbon Dates and Gene Sequences from Individual Hairs

Genetic data from archaeological specimens provides a potent new approach for addressing questions in prehistory. Hair from archaeological sites is an important but overlooked data source amenable to molecular analysis. To date DNA identification from hair is limited to modern samples. Our study is the first to report DNA recovery from a 9800-year-old specimen. We report methods used to extract mitochondrial DNA and obtain accelerator mass spectrometry radiocarbon dates (AMS-¹⁴C) from hairs plucked from a piece of ancient bighorn sheep (Ovis canadensis nelsoni) skin found at Smith Creek Cave, Nevada. The ability to obtain reliable radiocarbon dates and DNA sequences from single ancient hairs opens new possibilities for addressing biological questions in prehistory.     

Resource depression, climate change, and mountain sheep in the eastern Great Basin of western North America

Strontium, oxygen, and carbon stable isotope analysis may be used in conjunction with archaeofaunal data to identify resource depression by demonstrating that prey were obtained from more distant locations. We use fauna from Five Finger Ridge, a Fremont site in central Utah, to demonstrate that relative abundances of mountain sheep (Ovis canadensis) declined during a period of increased summer precipitation. Strontium ratio values from this period indicate that sheep were acquired from different locations than the preceding period. Specimens from this period also show a moderate increase in carbon ratio values, suggesting that mountain sheep were acquired from higher altitudes. Oxygen isotopes do not vary between temporal periods, possibly the result of the countering effects of higher oxygen isotope values associated with increased summer temperatures and lower oxygen isotope values present at higher elevations. Collectively, these data support that there were localized population declines of mountain sheep that may be related to either climatic changes or hunting pressure.     

Black Cyclope neritea Marine Shell Ornaments in the Upper Palaeolithic and Mesolithic of Franchthi Cave,Greece: Arguments for Intentional Heat Treatment

Abstract

The prehistoric site of Franchthi Cave yielded an exceptionally rich collection of personal ornaments. A reassessment of the Palaeolithic and Mesolithic ornaments from the site led to the hypothesis that a variable fraction of at least one type of personal ornaments, i.e. marine shell beads belonging to the species Cyclope neritea, may have been intentionally heated to change their natural whitish color to black. The limited conditions in which blackening can occur through heating, as well as comparison with the percentage of burnt land snails, animal bones, fish bones, and marine molluscan food remains in the Upper Palaeolithic and Mesolithic sequence, supports a special heat treatment for Cyclope neritea shells at Franchthi Cave.     

Bighorn hunting,resource depression,and rock art in the Coso Range,eastern California: a computer simulation model

The extraordinary record of prehistoric rock art depicting tens of thousands of animal images in the Coso Range of eastern California provides an opportunity to study the relationship between aboriginal hunting, forager ecology, bighorn prey population levels, and the production of rock art. We review archaeofaunal evidence that the Coso desert bighorn sheep population was strongly depleted during the Newberry era after 1500 B.C. We discuss the dating of the rock art and show a correlation between bighorn depletion and increased rock art production. These data are consistent with the arrival of Numic foragers ca. A.D. 600 who competed with the Coso Pre-Numics and eventually terminated the Coso rock art tradition. An ecological predator-prey computer simulation of the human populations (Numic and Pre-Numics), the sheep population, and the rock art “population”, demonstrates these proposed interconnections and gives a reasonable fit to the observed rock art production rate.     

Sorting the Sheep from the Goats: Morphological Distinctions between the Mandibles and Mandibular Teeth of AdultOvis and Capra

Diagnostic criteria are described for the taxonomic discrimination of adult mandibles of sheep (Ovis aries) and goat (Capra hircus). These criteria, based on morphological distinctions in the mandible, permanent premolars (P3–P4) and molars (M1–M3), have been successfully tested on a large and diverse body of modern reference material. In conjunction with published work on deciduous premolars, the new criteria enable complete and fragmentary mandibles of young, adult and elderly individuals to be identified to sheep or goat with acceptable levels of accuracy.     

DNA analysis of archaeological sheep remains from China

Recent research has thrown considerable light on the history of the domestic sheep, but has not extended to ancient sheep specimens. In the present study, ancient DNA analysis was carried out on eight archaeological sheep remains recovered from Erlitou archaeological site in Henan Province (ca. 2100–1800 B.C.) to explore the genetic structure of ancient sheep and the phylogenetic relationship between ancient and modern sheep. We analyzed the control region sequences and coding regions of mitochondrial DNA from the remains by direct sequencing and restriction fragment length polymorphism analysis, respectively. Our results reveal that all ancient sheep belong to lineage A defined by modern sheep sequences. Phylogenetic analysis shows that neither argali (Ovis ammon) nor urial (Ovis vignei) mtDNA is closely related to Erlitou ancient sheep. In addition, our results suggest that ancient DNA analysis can serve as a powerful tool in tracing prehistoric population movement.     

Pine Nut Use in the Early Holocene and Beyond: The Danger Cave Archaeobotanical Record

Nuts of limber pine (Pinus flexilis) from Early Holocene strata in Danger Cave, Utah, are distinguishable by seed-coat sculpturing from pine nuts of single-needled pinyon (Pinus monophylla), which occur in strata dating <7000 years . Owls and other taphonomic agents may deposit pine nuts in archaeological sites, but the morphology of the pine nuts in Danger Cave strongly indicate they were deposited by human foragers who brought small quantities with them for food for at least the last 7500 years. Large-scale transport of pine nuts to Danger Cave from distant hinterlands is unlikely, however. The seamless transition from limber pine to pinyon pine nuts in the Danger Cave record suggests that foragers who had utilized limber pine as a food resource easily switched to using pinyon pine nuts when pinyon pine migrated into the region at the close of the Early Holocene.     

Separating Sheep (Ovis aries L.) and Goats (Capra hircus L.) Using Geometric Morphometric Methods: An Investigation of Astragalus Morphology from Late and Final Bronze Age Central Asian Contexts

Many qualitative and quantitative methods for the separation of sheep and goat bones are based upon Middle Eastern, Mediterranean and European specimens. However, these methods may not be as applicable in other geographical contexts due to regional morphological variation. In order to address this, both traditional and geometric morphometric methods were applied to sheep, (Ovis aries L.) and goat (Capra hircus L.) astragali from archaeological sites from Kazakhstan dating to the Late and Final Bronze Age (1900–900 bce ). This exploratory research confirmed that qualitative features remained useful for distinguishing between species, while traditional morphometric methods were unable to conclusively support the field identification of species. Geometric morphometric methods found significant morphological differences between species and confirmed group membership while exploring specific qualitative features that were effective in distinguishing between sheep and goat in Central Asian contexts. Copyright © 2016 John Wiley & Sons, Ltd.     

Radiographic evidence for changing patterns of animal exploitation in the Southern levant

Radiographs were taken of sheep (Ovis aries) and goat (Capra hircus) metacarpals from archaeological sites in Israel dating from the Pre-Pottery Neolithic B (PPNB) to recent times. From these radiographs the combined cortical thickness (CCT) of the bones was measured, and used to estimate sample variation in bone mass. The samples were compared by species and period, and further compared with CCT values obtained for a recent sample of ibex (Capra ibex nubiana) from the same region. For all periods studied, CCT values in goats were comparable with those of recent ibex. A significant reduction in CCT values of both sheep and goats was found in the Early Bronze to recent samples as compared with the Chalcolithic and PPNB samples. It is proposed that this reduction in CCT values is associated with the presence of older, female animals in the later samples studied, and may be indicative of an emphasis on milk herds.     

The application of ancient DNA analysis to identify neolithic caprinae: a case study from the site of Hatoula,Israel

The evidence for wild sheep (Ovis orientalis) in archaeological sites from the southern Levant is examined through the application of ancient DNA analysis to specimens from the Pre‐Pottery Neolithic A (8300–7500 uncal. BC) site of Hatoula, Israel. The results indicate that at least one of the bones from this site, previously identified as sheep, is in fact goat. To date this is the earliest faunal sample to have yielded DNA in the region. This study highlights the problems in applying morphological criteria to distinguish between caprine species, and illustrates how ancient DNA analysis can serve as a powerful tool in resolving questions of species attribution. Copyright © 2003 John Wiley & Sons, Ltd.     

Analysis of enamel ultrastructure in archaeology: The identification of Ovis aries and Capra hircus dental remains

Ultrastructural features of deep, intermediate and superficial enamel were quantitatively assessed for the mandibular first permanent molars of 10 specimens each of the domestic sheep (Ovis aries) and goat (Capra hircus). The means of a number of variables differed interspecifically at high levels of statistical significance, especially with regard to the enamel of intermediate depth. A canonical discriminant function analysis employing nine variables at all three enamel depths resulted in the correct taxonomic assignment of 95% of the specimens comprising the present sample. These results, coupled with the simple preparative and analytical methods presented here, may enable archaeozoologists to utilize enamel ultrastructure in order to discriminate between fragmentary dental remains of these caprine species.     

Late Holocene Historical Ecology: The Timing of Vertebrate Extirpation on Crooked Island,Commonwealth of The Bahamas

We report eight new accelerator-mass spectrometer (AMS) radiocarbon (¹⁴C) dates performed directly on individual bones of extirpated species from Crooked Island, The Bahamas. Three dates from the hutia (Geocapromys ingrahami), recovered from a culturally derived bone assemblage in McKay's Bluff Cave (site CR-5), all broadly overlap from AD 1450 to 1620, which encompasses the time of first European contact with the Lucayan on Crooked Island (AD 1492). Marine fish and hutia dominate the bone assemblage at McKay's Bluff Cave, shedding light on vertebrate consumption by the Lucayans just before their demise. A fourth AMS ¹⁴C date on a hutia bone, from a non-cultural surface context in Crossbed Cave (site CR-25), is similar (AD 1465 to 1645) to those from McKay's Bluff Cave. From Pittstown Landing (site CR-14), an open coastal archaeological site, a femur of the Cuban crocodile (Crocodylus rhombifer) yielded an AMS ¹⁴C date of AD ～1050–1250, which is early in the Lucayan cultural sequence. From a humerus in a non-cultural surface context in 1702 Cave (site CR-26), we document survival of the Cuban crocodile on Crooked Island until AD ～1300–1400, which is several hundred years later than the well-documented extinction of Cuban crocodiles on Abaco in the northern Bahamas. We lack a clear explanation of why Cuban crocodiles likely survived longer on Crooked Island than on a larger Bahamian island such as Abaco. One AMS ¹⁴C date on Crooked Island's extinct, undescribed species of tortoise (Chelonoidis sp.) from 1702 Cave is BC 790 to 540 (2740 to 2490 cal BP), which is ～1500–1700 years prior to human arrival. A second AMS ¹⁴C date, on a fibula of this tortoise from McKay's Bluff Cave, is AD 1025 to 1165, thereby demonstrating survival of this extinct species into the period of human occupation.     

Camp Composition and the Rocky Mountain Fur Trade: Estimating the Native Presence at the Early Rendezvous and Winter Camps, 1825–1830

Rendezvous and winter camps were central to the early Rocky Mountain fur trade. However, available research provides no estimate of the number of people in attendance. Knowledge of the size and demographic makeup of the mountain gatherings would facilitate research on camp socio-cultural dynamics, interpretation of the historic events, and identification of modern archeological sites. The present study estimates the number of people present at the early rendezvous and winter camps (1825–1830). The estimations support existing research that emphasizes Native people's involvement in the fur trade. For instance, of the 1550 estimated attendees at the 1827 rendezvous, the Native contingent comprised approximately 90% of the total. While it is commonly known that Native peoples attended and participated in the Rocky Mountain fur trade rendezvous, this research presents evidence that the Native presence often far outnumbered the traders and trappers in attendance.     

A radiographic study of the extent of variation in cortical bone thickness in soay sheep

In order to assess the effect of nutrition on bone mass, radiographs of metopodia from skeletal collections of Soay sheep (Ovis aries) were taken of animals subject to seasonal stress (from the island of Hirta, Hebrides) and those living in good nutritional conditions (from the Institute of Animal Physiology, Babraham, Cambridge). A third set of radiographs was taken at 3 month intervals over a single year on living Soay sheep kept under good conditions at the Rowett Research Institute, Aberdeen.Measurements were taken on the radiographs of length, minimum shaft width (SW) and medullary cavity width (MCW), while combined cortical thickness (CCT) was calculated. The ewes from Hirta had significantly lower values for CCT than those from Babraham, whereas the rams showed no significant reduction in CCT. Since no seasonal variation in CCT was detected in the well nourished Rowett ewes, this indicates a differential effect of poor environmental conditions on bone metabolism in ewes. It is proposed here that this may be related to the additional stress imposed by gestation and lactation on female animals living under conditions of seasonally restricted food supply.     

A dental microwear study of seaweed‐eating and grazing sheep from Orkney

The potential of dental microwear for recognizing the use of seaweed as fodder in the past is explored through the analysis of microwear patterning in modern seaweed‐eating and grazing sheep from Orkney. Seaweed‐eating and grazing sheep are clearly distinguished on the basis of microscopic dental wear patterns. This reflects an emphasis on anterior‐posterior jaw movements and large pitted features in the seaweed‐eaters and can be attributed to the differing forces and/or masticatory movements required in the comminution of grasses and seaweed. Differences between seaweed‐eating and grazing individuals are maintained when the grazing group is expanded to include grazing sheep from Greenland and the Scottish borders. It is concluded that the microwear of seaweed‐eating sheep is highly distinctive and that dental microwear analysis potentially has a widespread application for identifying seaweed grazing within Scotland and the North Atlantic islands. Copyright © 2000 John Wiley & Sons, Ltd.     

Diet and death in times of war: isotopic and osteological analysis of mummified human remains from southern Mongolia

This study presents the results of an isotopic analysis of nine naturally mummified individuals—three adults, two adolescents, one juvenile, and three infants—recovered from the Hets Mountain Cave site in southern Mongolia, where they had been secondarily deposited. All of the individuals show evidence of violent perimortem trauma, but no skeletal indicators of nutritional or disease-related stress. Multi-isotopic data (δ¹³C, δ¹⁵N, δ¹⁸O, ⁸⁷Sr/⁸⁶Sr, and ²⁰ⁿPb/²⁰⁴Pb) were characterized in multiple tissues from each individual when possible, in order to reconstruct diet composition and residential origin at different points in life. Specifically, δ¹³C and δ¹⁵N in bone carbonate and collagen (N = 8) and hair keratin (N = 4) were coupled with enamel carbonate δ¹⁸O and δ¹³C (N = 3) and enamel ⁸⁷Sr/⁸⁶Sr, and ²⁰ⁿPb/²⁰⁴Pb (N = 3) to assess diet and residential mobility in relation to skeletal indicators of health and trauma. Results are consistent with a persistence of mixed C₃/C₄ pastoral subsistence and general stability of diet composition over the life course, in contrast to contemporary accounts of widespread famine and a dependence on grains imported from China throughout the region. However, results also suggest that at least some individuals may have migrated to this region of southern Mongolia from elsewhere during life, meaning that their dietary isotopic profiles may not represent local subsistence patterns near the Hets Mountain Cave site. Overall, these results speak to the utility of life course oriented multi-isotopic analysis in complementing more top-down historical analyses in understanding variation in subsistence, nutrition, and migration in regions undergoing significant political and economic turmoil.     

Skeletal markers of occupational stress in the Fur Trade: A case study from a Hudson's Bay Company Fur Trade post

The Seafort Burial Site (FcPr100) is a Fur Trade Period cemetery located near the town of Rocky Mountain House, Alberta. The results of recent analysis of skeletons recovered during salvage excavations between 1969 and 1971 suggest that three males in this sample were voyageurs in the employ of the Hudson's Bay Company. Vertebral osteophytosis and osteoarthritis, Schmorl's nodes, muscle origin robusticity and new bone formation at entheses and syndesmoses, and osteoarthritis at the elbow and shoulder joints provide evidence for carrying, lifting, and paddling or rowing. The presence of accessory sacral facets also may be stress-related. In addition, the development of accessory articular facets on the metatarsals and proximal pedal phalanges may indicate activity-induced stress from hyperdorsiflexion of the toes in habitual kneeling, such as when river canoeing.     

A re-assessment of Basketmaker II cave 7: massacre site or cemetery context

Our ongoing investigation of early maize farming in the American Southwest has entailed stable isotope analysis and accelerator radiocarbon dating of Basketmaker II remains from sites in the Four Corners region. Here we report radiocarbon dates on a large mortuary assemblage excavated by Richard Wetherill in 1893 from a burial cave in southeastern Utah. It has long been thought that all individuals interred in Cave 7 were massacred in a single violent attack given embedded projectiles and evidence for blunt force trauma. However, accelerator radiocarbon dates on purified bone collagen (n = 96) do not lend strong support to this argument, even among the subset of individuals with clear evidence for violent injuries. Moreover, nearly 80% of Cave 7 burials examined in the study show no evidence of perimortem trauma and no adult females or subadults under the age of 12 appear to have suffered violent deaths. Rather than an anomalous single-event massacre, the Cave 7 radiocarbon dataset suggests that raiding and intra-group, male/male violence was episodic among Basketmaker groups in southeastern Utah. Population densities were relatively high and individuals interred in Cave 7 and elsewhere in the region were heavily dependent on maize agriculture, a prehistoric economic strategy typically characterized by high amplitude fluctuations in productivity. Variability in the array of grave goods accompanying Cave 7 male burials and elsewhere suggests competitive social differentiation likely heightened during periods of resource shortfall leading to intra-group conflict, raiding and perhaps ritualized acts of violence.     

An archaeologically validated protocol for computing obsidian hydration rates from laboratory data

This paper reports the computation of hydration rate for Topaz Mountain obsidian from laboratory data, and a comparison with archaeological data from a well-dated site, Camels Back Cave, in western Utah. Topaz Mountain obsidian is found to be slow-hydrating, with a rate of 0.071 ± 0.021 μ/yr^½ at an effective hydration temperature of 16.01 °C. This rate agrees with a rate developed from archaeological data from Camels Back Cave within ∼6%. Activation energy of Topaz Mountain obsidian is 10370 ± 544 K, and its diffusion constant is (1.87 ± 9.13) × 10¹³ μ²/yr, both of which are independent of temperature. Its intra-source variability in hydration rate is very low (CV < 0.01), implying a low variability in intrinsic water. We present a model of chemical erosion which shows why earlier laboratory-determined rates were incorrect, and discuss the implications of our findings on the determination of experimentally derived rates in obsidian hydration dating.     

Late Neolithic household activities in marginal areas: the micromorphological evidence from the Kouveleiki caves,Peloponnese, Greece

The micromorphological study of the Neolithic archaeological sequence of the Kouveleiki Caves A and B and the reassessment of the archaeological record under the new data revealed that the site constituted a small-scale self-contained mixed farming household in an upland area of Greece. The study of the microstructure and the microstratigraphy of the sediment revealed that the front chamber of Cave A is dominated by dry, almost totally burnt coprolites resulting from stabling, most likely of sheep and/or goats. There are also indications of frequent trampling and disturbance of the stabling deposits by human activities. Hence, Cave A was used as a small seasonal pen and, probably, as a place for rudimentary activities. The dark back chamber of Cave A was the main habitation area, where plastered floors were constructed by a mixture of burnt dung (derived from the seasonal pen) and red clay (to be found in the plain in front of the cave). The nearby Kouveleiki Cave B was probably used as a complementary activity area.