Protocol for Recording Enamel Hypoplasia in Modern and Archaeological Caprine Populations

This paper outlines the first methodology for recording dental enamel hypoplasia in the high‐crowned dentition of modern and archaeological caprine teeth. The method has been developed and trialed on five caprine populations from Orkney (UK); two modern populations (Shetland and North Ronaldsay breeds) and three Neolithic assemblages from the archaeological sites of Knap of Howar, Skara Brae and Holm of Papa Westray. Problems associated with differential tooth wear, as well as the presence of coronal cementum, are discussed, and recommendations are given on the identification and recording of hypoplastic dental defects in caprines. Copyright © 2012 John Wiley & Sons, Ltd.     

Ancient DNA evidence for genetic continuity in arctic dogs

The domestic dog fulfills many functions for their human companions, such as hauling, guarding, and protection. Consequently, humans have taken dogs to nearly every corner of the globe. Recent translocations of Western dogs stemming from the Victorian era dog fancy have erased some of the ancient genetic signatures of these earlier migrations. Here we used DNA of ancient and modern dogs from the North American Arctic of Alaska and Greenland to assess their genetic continuity in time and space. We successfully sequenced 23 archaeological (ca. AD 1250–1910) and recent (ca. AD 1930–1990) surface-collected Canis bone and tooth samples and compared them to 51 modern Inuit Sled Dogs and to published sequences of modern Alaskan Malamutes (and additional Inuit Sled Dogs) to test for evidence of lineage replacement or genetic continuity through time. Ancient samples from Alaska and Greenland and modern sequences from Greenland all contained a high frequency of haplotype A31, which was previously described only in modern North American Arctic dogs. Thus, A31 was a common thread tying the entire North American Arctic together prior to European colonization and, in the Eastern Arctic, indicates genetic continuity between past and present dogs as well. However, A31 is rare in modern Alaskan dogs, consistent with post-colonization replacement by Eurasian matrilines.     

Fruits of Their Labour: Urbanisation,Orchard Crops,and Dental Health in Early Bronze Age Jordan

The analysis of dental remains, which outlast most other tissues in the human body, provides insight into past diet, activity patterns and ancestry. The remains from Bab edh‐Dhra' represent the only skeletal sample available to assess the impact of agricultural intensification in the Early Bronze Age of the southern Levant (ca. 3500–2000 bce ). This era ushered in a period of ‘urbanisation’, evidenced by fortified towns, planned roadways, developments in irrigation and growing population density. During this time, the cultivation, trade and consumption of orchard taxa (such as figs, grapes and olives) increased. This paper examines changes in the teeth associated with agricultural intensification involving orchard crops as well as grains. Dental caries, ante mortem tooth loss and dental wear are examined for Early Bronze IA (EBIA; 3500–3300 bce ) and Early Bronze II–III (EBII–III; 3100–2300 bce ) teeth from the site of Bab edh‐Dhra', located in modern‐day Jordan. Due to the commingling, general tooth groups (e.g. molars) and specific tooth types (e.g. lower left canine) were used to compare periods. Although age and sex could not be identified for every tooth, analyses of crania and os coxae showed no significant difference in demographic profiles of EBIA and EBII–III. No statistically significant increase was found over time in dental caries frequency; however, teeth for which the cause of pulp exposure could be determined suggested that caries increasingly led to exfoliation. Indeed, ante mortem tooth loss rose significantly with time, whereas dental wear decreased. In general, changes in oral health were consistent with an archaeological record of greater consumption of softer, stickier foods, such as fruits. Copyright © 2013 John Wiley & Sons, Ltd.     

Recording dental caries in archaeological human remains

Dental caries is an important condition to record in archaeological collections, but the way in which recording is carried out has a large effect on the way in which the results can be interpreted. In living populations, dental caries is a disease that shows a strong relationship with age. Both the nature of carious lesions and their frequency change with successive age groups from childhood to elderly adulthood. There is also a progression in the particular teeth in the dentition which are most commonly affected and, in general, the molars and premolars are involved much more frequently than the canines and incisors. Lower teeth are usually affected more than upper, although the condition usually involves the right and left sides fairly equally. In the high tooth wear rate populations represented by many archaeological and museum collections, there is a complex relationship between the form of lesions and the state of wear, which adds yet another range of factors to the changing pattern of caries with increasing age. In the same populations, chipping, fracture and anomalous abrasion of teeth are also common, and these contribute similarly to the distribution and forms of carious lesion observed. Amongst the living, the pattern of ante‐mortem tooth loss is important in understanding caries and, in archaeological material, there is also the complicating factor of post‐mortem tooth loss. Finally, there is the question of diagnosis. There are diagnostic problems even in epidemiological studies of living patients and, for archaeological specimens, diagenetic change and the variable preservation of different parts of the dentition add further complications. For all these reasons, it is difficult to define any one general index of dental caries to represent the complete dentition of each individual, which would be universally suitable for studying a full range of collections from archaeological sites or museums. Variation in the nature of collections, their preservation, tooth wear, and ante‐mortem and post‐mortem tooth loss mean that when such a general index appears to differ between sites, there could be many other reasons for this, in addition to any genuine differences in caries incidence and pattern that might have been present. It is suggested here that the best approach is instead to make comparisons separately for each tooth type, age group, sex, lesion type and potential lesion site on the tooth. Copyright © 2001 John Wiley & Sons, Ltd.     

Trauma and pathology of a buried dog from San Nicolas Island,California, U.S.A.

To date more intact dog remains have been found on San Nicolas than on any of the other seven California Channel Islands. However, little is known about them. During the 2007 summer field season we excavated a medium sized young male dog in a flexed burial position from a pit at CA-SNI-25, a large Native American village site. The dog exhibits hypodontia, noticeable tooth attrition, severe scapular trauma, and vertebral, rib, and phalanx pathologies. Most of the injuries appear to have resulted from a severe blunt force trauma of unknown origin and it is likely the dog would not have survived without human care. The results of our analysis contribute to the, somewhat limited, published data on archaeological dogs in California. In this paper we describe the biological characteristics of the dog especially his anomalies and pathologies and compare them with published reports of other North American archaeological dogs with similar conditions.     

Determining Age and Season of Death by Use of Incremental Lines in Norwegian Reindeer Tooth Cementum

Using incremental patterns in tooth cementum is a powerful tool for age assessment. Recent developments have shown that the method has a large potential as an indicator of the season of death of the individual in question. In this paper, the results of a study comparing thin sections of teeth of the modern Hardangervidda reindeer population to those of Iron Age, eleventh, and thirteenth century reindeer hunting stations from the same area are presented. The tooth wear stages were compared to the actual age of the individual (based on the number of incremental lines and the age at eruption). It was established that in the studied reindeer populations wear stages sometimes result in too low an age estimate. Having identified the start of the period of deposition for rest lines in the modern reindeer population as early autumn and winter, analysis of the outermost layer in the tooth cementum at the archaeological sites points to a prolonged hunting season in the thirteenth century, while in the earlier phases hunters were probably present on fewer and shorter visits.     

Problems and pitfalls in the diagnosis of linear enamel hypoplasia in cheek teeth of cattle

Prevalence and intensity of enamel hypoplasia have been used as markers of generalized physiological stress during dental development in a wide range of mammalian taxa. We studied cattle (Bos taurus) cheek teeth exhibiting morphological characteristics that are of relevance to the diagnosis of enamel hypoplasia in this and other bovid species. These characteristics were multiple, more or less horizontally arranged (waveform) lines or grooves in the cementum of the tooth crown and the adjacent root area, leading to an imbricated appearance of the cementum. On macroscopic examination of tooth surfaces, these lines resembled linear enamel hypoplasia (LEH). Microscopic analysis of tooth sections, however, revealed that the lines occurred in the cementum only, and that the underlying enamel did not exhibit morphological irregularities. In cheek teeth of older cattle, a thick cementum layer is regularly found in the cervical crown portion and the adjacent root area. Apposition of this cementum is related to the uplifting of the teeth from their alveoli, a process that compensates for the shortening of the tooth crowns due to occlusal wear. In the studied specimens, a pronounced periodic nature of tooth uplifting and the related deposition of cementum is the likely cause for the observed imbricated appearance of the cementum. While this phenomenon may be misinterpreted as representing a case of LEH, presence of enamel hypoplasia in bovid teeth may be overlooked when the defects become filled with coronal cementum and are therefore not apparent on external inspection. This was the case in one of the cattle teeth analyzed by us, in which the hypoplastic enamel defects were, however, clearly discernible in ground sections. Microscopic analysis of tooth sections is recommended for recording of LEH in bovid teeth in cases where macroscopic examination of tooth surfaces alone does not produce unequivocal results.     

Hypodontia of permanent teeth among Middle Palaeolithic hominids: an early case dated to ca. 92 000±5000 years BP at the Qafzeh site

The analysis of the maxillae and mandible belonging to a Middle Palaeolithic child has provided evidence of the early appearance of a developmental dental anomaly among Homo sapiens. The material came from the Near Eastern site of Qafzeh. In the mandible there was agenesis of the left second premolar and congenital absence of the right could not be totally excluded. Additional analyses including two presumed cases of dental development anomalies within contemporaneous prehistoric populations showed that tooth agenesis also can affect the anterior region of the mandible, and could be clearly separated from ante-mortem tooth loss caused by trauma. © 1998 John Wiley & Sons, Ltd.     

The frequency and distribution of dental caries and tooth wear in a Byzantine population of Sa'ad,Jordan

The aim of this study was to determine the frequency, distribution and extent of dental caries and tooth wear in a Byzantine population in Sa'ad, to compare these with modern Jordanians, and to draw inferences about their lifestyle. Dental caries and tooth wear were examined in 1159 permanent teeth using direct vision and dental explorer. The location and extent of the lesions were recorded and evaluated according to defined criteria. The overall caries prevalence was 16.8% (n = 1159). Most of the carious lesions (13. 9%, n = 1159) were present in the tooth crown, on occlusal or proximal surfaces; 2.9% of teeth displayed root caries alone. The upper and lower central incisors exhibited the lowest coronal caries frequency, while molars showed the highest frequency. Attrition with moderate dentine exposure was evident in most teeth. Some teeth (9.8%) demonstrated oblique abrasion and 9 (0.78%) teeth exhibited cupped occlusal surfaces. The prevalence of caries was within the range reported for other Byzantine populations and lower than that of modern Jordanians. The attrition seen in most teeth and the cupped occlusal surfaces might be due to eating abrasive food, drinking wine or acidic beverages. Oblique abrasion on the facial and palatal surfaces reflects their business or craft, such as basket making and leather processing. Copyright © 2008 John Wiley & Sons, Ltd.     

Using plant microfossils from dental calculus to recover human diet: a case study from Tell al-Raqā'i,Syria

Dietary reconstructions based on plant microfossils, such as starch grains and phytoliths, have been useful in increasing our understanding of past human populations. Microfossils have been recovered from sediments, stone tools, and, more recently, dental calculus. Methods for recovering microfossils from dental calculus have yet to be firmly established and there is some question about potential damage to the teeth. Using a sample of teeth from the middle Holocene site of Tell al-Raqā'i, Syria, we tested using a dental pick to sample the calculus. ESEM images taken before and after sampling show no damage to the enamel surface, and examination of the recovered microfossils show that this method provides ample material for study, even when not all of the calculus is removed from the tooth. Preliminary identification of the plant microfossils suggests that these individuals were consuming a variety of plant foods, but that domesticated cereals such as wheat and barley made up a surprisingly small portion of their diet.     

Palaeopathology in late Pleistocene and early Holocene Central Plains bison: dental enamel hypoplasia,fluoride toxicosis and the archaeological record

Multiple formative agents are cited for dental enamel hypoplasia (DEH) in modern and fossil mammals. Here, four late Pleistocene-early Holocene bison assemblages from the Central Great Plains are analyzed for DEH and other bone pathologies to investigate the possible influence of chronic fluoride toxicosis, a condition that may have presented a long-term source of duress for prehistoric bison and devalued them as nutritional resources to human hunters. Although DEH is present in all the analyzed assemblages, the lack of corroborating dental and post-cranial anomalies consistent with chronic fluoride toxicosis speaks against it as a primary factor of DEH formation or general ill health in these bison. Rather, maternal nutritional stress, weaning, and seasonal forage condition are invoked as probable promoters of DEH formation. Other identified abnormalities are discussed in reference to their contribution to reduced feeding efficiency and lameness, as are their roles in developing palaeoecological models applicable to archaeological questions about ancient human lifeways.     

Size and shape of the Eurasian wild boar (Sus scrofa), with a view to the reconstruction of its Holocene history

Abstract

A large assortment of skulls and skeletons of recent wild boar (Sus scrofa) from across the world has been used to collect tooth and bone measurements that can be compared to those from archaeological specimens. The data provide useful information for a reconstruction of the Holocene history of the species. The evidence collected so far highlights the great variability of the species and provides a baseline to be used for the interpretation of ancient material. It is shown that not only the size, but also the shape of teeth and mandibles can help in highlighting patterns of variability in wild boar from different areas. A number of geographic trends are identified in the variation of S. scrofa across its range, mainly concerning the differentiation of insular forms, and the existence of South–North and West–East clines. Other factors such as hybridisation with domestic stock, feralisation and human-induced movement of animals may also play an important role. A comparison with ancient material emphasises the existence of similarities as well as differences between modern and ancient populations. Although some of the geographic trends identified on the basis of the analysis of modern material seem to date back to early Holocene times, the morphological history of the species appears to be complex, and in more than one area fluctuations in body size seem to have occurred.     

Absolute age and tooth eruption and wear sequences in sheep and goat: determining age-at-death in zooarchaeology using a modern control sample

This paper seeks to improve our understanding of the relationship between mandibular tooth eruption and wear sequences and absolute age in modern sheep and goat. While the relationship between tooth eruption and wear and absolute age has been investigated for primitive breeds in parts of the Old World, our pilot study extends the applicability of this research to modern breeds and to another geographic region (North America). A modern control sample of two species of domestic caprines – Ovis aries (sheep) and Capra hircus (goats) from Manitoba, Canada – are used to evaluate the two major systems for analyzing mandibular tooth wear and eruption (Payne and Grant) and for assigning an absolute age-at-death. In order to compare the two systems, an absolute age for Grant's Mandibular Wear Stages was established for the first time, previously demonstrated only for Payne's system. The analysis confirmed that there is a very high correlation between the known age and estimated age based on tooth eruption and wear. However, Payne's system generated far coarser age-at-death profiles, which lowers its utility in constructing harvest profiles. Grant's system is recommended for ageing be utilized since it can yield more sensitive age estimations. In conclusion, it is possible to utilize the modern caprine breeds to establish the relationship between tooth eruption and wear and absolute age to reconstruct age and season of death of archaeological samples.     

Measuring Root Dentine Translucency in Human Teeth of Varying Antiquity

Many workers have calibrated human root dentine transparency (RDT) as a linear regression on age. It is now regarded as a well-established means of estimating age at death in modern human material. Similar applications in archaeological material have not yet been developed. The aim of this study was to establish a standard protocol for measuring RDT which was derived from previous methods and which could be applied to teeth of unknown and varying antiquity. An initial study on two archaeological populations determined the choice of tooth to study and a second study, using expendable teeth (of unknown age and origin), evaluated various techniques of specimen preparation and examination. Findings from the pilot study indicated that the lower canine was the tooth of choice. From the second study it was observed that archaeological teeth could only be sectioned if they had first been infiltrated and embedded in methyl methacrylate. The optimal section thickness was found to be 150 μm and no benefit was gained by staining. Inter-observer reliability tests showed significant differences in repeated measures of RDT in intact teeth, which were not borne out when sectioned teeth were used. Intra-observer reliability was maintained for measurements in both intact and sectioned teeth. These findings have been used to establish a standard protocol for application to human teeth of any depositional phase to estimate the dental age at death of that individual.     

Dental trauma and antemortem tooth loss in prehistoric Canary Islanders: prevalence and contributing factors

Differential diagnosis of the aetiology of antemortem tooth loss (AMTL) may yield important insights regarding patterns of behaviour in prehistoric peoples. Variation in the consistency of food due to its toughness and to food preparation methods is a primary factor in AMTL, with dental wear or caries a significant precipitating factor. Nutritional deficiency diseases, dental ablation for aesthetic or ritual reasons, and traumatic injury may also contribute to the frequency of AMTL. Systematic observations of dental pathology were conducted on crania and mandibles at the Museo Arqueologico de Tenerife. Observations of AMTL revealed elevated frequencies and remarkable aspects of tooth crown evulsion. This report documents a 9.0% overall rate of AMTL among the ancient inhabitants of the island of Tenerife in the Canary Archipelago. Sex‐specific tooth count rates of AMTL are 9.8% for males and 8.1% for females, and maxillary AMTL rates (10.2%) are higher than mandibular tooth loss rates (7.8%) Dental trauma makes a small but noticeable contribution to tooth loss among the Guanches, especially among males. In several cases of tooth crown evulsion, the dental root was retained in the alveolus, without periapical infection, and alveolar bone was in the initial stages of sequestering the dental root. In Tenerife, antemortem loss of maxillary anterior teeth is consistent with two potential causal factors: (a) accidental falls while traversing volcanic terrain; and (b) interpersonal combat, including traditional wrestling, stick‐fighting and ritual combat. Steep‐walled valleys (barrancos) and lava fields (malpaís) required agile locomotion and occasional vaulting with the aid of a wooden staff. Accidental falls involving facial injury may have contributed to AMTL. Traditional conflict resolution involved competitive wrestling (lucha canaria), stick‐fighting (juego del palo), and ritualised contests involving manual combat. These activities made a small but recognisable impact on anterior dental trauma and tooth loss. Inter‐personal behaviours of such intensity leave their mark on skeletal and dental remains, thereby providing insight into the lives and cultural traditions of the ancient Guanches. Copyright © 2006 John Wiley & Sons, Ltd.     

Assessing the reliability of criteria used to identify mandibles and mandibular teeth in sheep, Ovis, and goats, Capra

Criteria developed to distinguish between the mandibles and mandibular teeth of sheep and goats are evaluated using modern specimens from the collections of the National Museum of Natural History and the Field Museum of Natural History. Certain teeth prove to be quite reliable in this regard (dP3, P3, P4). Other dental elements, however, are not reliable and should not be used in dental based identifications of these closely related species (dP4, M1, and mandibular bones). Overall, the identification of sheep dentition using these criteria is more reliable than it is for goats. However, the generally greater likelihood of incorrect identification of goat teeth and mandibles means that specimens identified as sheep are likely to contain high proportion of misidentified goats. Both the proportion of remains that can only be classified as ‘Ovis-Capra’ and the proportion of incorrectly identified teeth and mandibular bones varies with age, with identifications of younger and older animals less reliable than those of animals in the 1.5–6 year age range. Reliability of identifications increases when more criteria and more elements are used. Identifications based on whole mandibles with complete tooth rows are quite reliable. However, the proportion of specimens likely to be classified as ‘Ovis-Capra’ also increases in whole mandible identifications, especially in goats. Systematic differences in the proportions of indeterminate and incorrect identifications have a significant impact on species-level dentition-based harvest profiles. Recommendations are made for the use of dentition and long bone based harvest profiles in the study of sheep and goat exploitation strategies.     

Cut and Tooth Mark Distributions on Large Animal Bones: Ethnoarchaeological Data from the Hadza and Their Implications For Current Ideas About Early Human Carnivory

Distributions of cut and tooth marks on the bones of large animals found in archaeological sites are increasingly used as sources of inference about the relative importance of hunting and scavenging in early human diets, and (by extension) about the role of meat-eating in human evolution. Here we review the empirical basis for these inferences in light of ethnoarchaeological data from the Tanzanian Hadza, a modern East African foraging population. Comparison of the Hadza data with those produced by other actualistic work indicates that while there may be a relationship between cut and tooth mark distributions and order of consumer access (human- versus carnivore-first), it is less clear-cut than many have suggested. Application of these results to the analysis of Plio-Pleistocene archaeological collections is further complicated by inconsistencies in the ways cut and tooth marks have been defined and counted, and by significant differences between patterns observed in modern control samples and those reported at ancient sites. These observations indicate that cut and tooth mark analyses are unlikely to speak effectively to questions about early human carnivory in the absence of: (1) better-warranted, more comprehensive expectations about the potential range of variation in past human carcass acquisition strategies, (2) a larger, more rigorously designed set of control experiments that model the archaeological consequences of these strategies, and (3) a larger, more consistently analysed archaeological data base. Even if these requirements are met, the idea of meat-eating as an important catalyst in the evolution of early humans will remain highly problematic, mainly due to problems involving the frequency and short-term reliability of carcass access.     

Dental Wear and Pathology in the Precolonial Caribbean: Evidence for Dietary Change in the Ceramic Age

This study investigates temporal changes in dietary practices in the Caribbean archipelago during the Ceramic Age (400 BC–AD 1500), through analyses of dental wear and pathology. Some previous studies in the region have suggested that diet and subsistence practices changed over time due to increasing sociopolitical complexity, climate change, or adaptation to island environments rich in marine resources. Both horticultural/agricultural intensification and increased marine focus of the diet over time have been posited, based among other things on faunal and botanical remains, and early ethnohistorical accounts. Local and micro‐regional stable isotope studies of temporal dietary variation have found few indications for change over time, and large regional isotope studies are still lacking. Dentitions from sites throughout the region dating to the Early Ceramic Age (400 BC–AD 600/800) and the Late Ceramic Age (AD 600/800–1500) were analysed in order to assess temporal differences. Intra‐individual rates of wear were calculated using the difference in degree of wear between the adjacent molars and the two groups were compared with principal axis analysis. Caries, antemortem tooth loss, abscesses and dental calculus were recorded per individual and per tooth/socket, and population caries and antemortem tooth loss rates were assessed and compared by age group, tooth class and sex. Comparisons between the two occupation periods revealed significant differences in the rate of dental wear and pathology, indicating a shift in dietary practices over time, coinciding with known social changes. The increase in pathology rates suggests a rise in the consumption of cariogenic foods or preparation techniques that increase cariogenicity. The decrease in rate of wear over time indicates a reduction in abrasivity of the diet. Together these data suggest that there was a growing focus on refined, cariogenic foods, likely horticultural/agricultural produce. Copyright © 2014 John Wiley & Sons, Ltd.     

The Miles Method of Assessing Age from Tooth Wear Revisited

Age-estimation methods tend to under-estimate advanced age and consequently life expectancy of populations. Many methods use age-scales ending in categories such as “50 years and over” without explaining how the upper limits are decided; the general belief that in early times life was short may play a part. Instances now exist of contemporary written records showing that, even when expectation of life is low, some people become septuagenarians or even older. Most methods of age-estimation, commencing with Gustafson's using dental criteria, predominantly root translucency, have recently been scrutinized by several workers but tooth wear as a method has escaped such scrutiny. A wide range of published findings based on tooth wear is therefore reviewed here. Most age-estimating criteria can only be assessed in terms of ordinal stages, unlike root translucency which can be measured on a continuous scale. Tooth wear ultimately ends in total loss of the dentition but, until that stage, has the potential, not yet fully developed, of also being so measured. The revision is reported of the 1989 estimated age-at-death distribution of a skeletal population. Based on tooth wear and tooth loss, the ages of most over 50 years of age were advanced by about 15 years and reasons are given for regarding four as likely to have been over 75 years of age. Palaeodemography today needs such survivors to be identified, however tentatively.     

Bison breeding characteristics and interpretation of archaeological seasonality revisited

Bison breeding behaviour has been used for the last three decades as the basis for developing methods for assigning season‐of‐occupation estimates to archaeological sites on the North American Plains. These methods are based upon the supposition that the North American bison breeding season is extremely short and that genetically controlled ontological characteristics such as foetal growth and tooth eruption sequences can therefore be used to infer site seasonality in a reasonably straightforward and precise manner. This paper reviews bison population studies conducted during the past 30 years to reassess present understandings of the length of North American bison breeding seasons. It is concluded that the bison breeding season regularly extends over a period of three to four months, and that initiation of ontological development will therefore also vary over the same period of time. Bison development characteristics cannot provide a reliable or precise means of assigning seasonality to archaeological sites on the North American Plains. It is suggested that methods using physical characteristics such as dental cementum incrementation which are directly affected by seasonal changes could be more productive. Copyright © 2006 John Wiley & Sons, Ltd.